large scale gene expression profiling focus on mirnas, ChIP-on-chip and whole genome sequencing", May, Turku, Finland

Transcrição

1 CURRICULUM VITAE LUIS F.S. GOULÃO PERSONAL DATA Full Name: Luís Filipe Sanches Goulão Nationality: Portuguese Birth Place: Carnaxide, Oeiras Date of Birth: July, 1973 Institutional Address: Instituto de Investigação Científica Tropical (IICT) Centro de Ecofisiologia, Bioquímica e Biotecnologia Vegetal Av. da República Oeiras - PORTUGAL Phone: goulao@iict.pt EDUCATION PhD IN AGRONOMIC ENGINEERING Plant Physiology and Molecular Biology INSTITUTO SUPERIOR DE AGRONOMIA, LISBON - PORTUGAL MSci IN SUSTAINABLE AGRICULTURE AND HORTICULTURE (Scholar Part) INSTITUTO SUPERIOR DE AGRONOMIA, LISBON PORTUGAL DEGREE IN AGRONOMIC ENGINEERING Plant Breeding INSTITUTO SUPERIOR DE AGRONOMIA, LISBON PORTUGAL ADVANCED TRAINING 2009 The Gulbenkian Training Programme in Bioinformatics Course: Workflows and Programatically Accessible Tools, November, Oeiras, Portugal 2009 The Gulbenkian Training Programme in Bioinformatics Course: Microarray Data Analysis using R and Bioconductor, September, Oeiras, Portugal European Science Foundation for Functional Genomics (ESFFG) Workshop: "Advanced large scale gene expression profiling focus on mirnas, ChIP-on-chip and whole genome sequencing", May, Turku, Finland Workshop "Coconut genetic resources management using microsatellite kit and dedicated statistical software", April, Montpellier, France TRAINING IN INTERNATIONAL LABORATORIES Visiting scholar at the Department of Genetics - University of Georgia, Athens, GA - USA (supervision of Prof. Chung-Jui Tsai and Dr. Scott Harding), September-November 2004 Visiting scholar at the Department of Biology - Pennsylvania State University, PA USA (supervision of Prof. Daniel Cosgrove), March-June 1

2 RESEARCH INTERESTS BIOTECHNOLOGY APPLIED TO ECONOMICALLY IMPORTANT CULTIVATED SPECIES - Identification, expression and functional characterization of genes with agronomic and industrial relevance in economically important plant species - Molecular, biochemical and enzymatic modifications of the plant cell wall - Postharvest biology and technology: fruit ripening, quality and storage - Plant tolerance mechanisms to multiple abiotic stresses - Applications of molecular markers in plant management, breeding and agriculture SCIENTIFIC ACTIVITIES SINCE JUL 2009: Researcher ( Investigador Auxiliar ) at Centro de Ecofisiologia, Bioquímica e Biotecnologia Vegetal - Instituto de Investigação Científica Tropical (IICT), Oeiras NOV JUN 2009: Post-Doctoral Fellow at Plant Cell Wall Group - Instituto de Tecnologia Química e Biológica (ITQB), Oeiras JAN OCT 2006: Graduate Student at Plant Cell Wall Group - Instituto de Tecnologia Química e Biológica (ITQB), Oeiras JUL MAR 2005: Research Grant for Program AGRO 8.1 at Instituto Superior de Agronomia (ISA), Lisbon / Central de Frutas do Paínho, Cadaval JUL JUL 2004: PhD Student at Secção de Horticultura - Instituto Superior de Agronomia (ISA), Lisbon JAN DEC 1999: Young Graduate Researcher Fellow at Instituto Superior de Agronomia (ISA), Lisbon / Faculdade de Engenharia e Recursos Naturais - Universidade do Algarve (FERN/UALg), Faro / Estação Nacional de Fruticultura Vieira Natividade (ENFVN), Alcobaça MAR DEC 1996: Estagiário at Departamento de Forragens e Proteaginosas - Estação Nacional de Melhoramento de Plantas (ENMP), Elvas PARTICIPATION IN RESEARCH PROJECTS NOV : PTDC/AGR-AAM/64078/2006: Molecular evaluation and characterization of tolerance mechanisms to adverse environmental conditions in Coffea sp. - central role of the oxidative stress control at leaf level. JAN 2005 JUN 2009: POCTI/AGR/46671/02: Cell wall proteins with roles in xylogenic programmes in eucalyptus. JAN MAR 2005: AGRO 518: Improving postharvest quality and safety of pear, apple and kiwi. JUL JUN 2004: POCTI/33733/99: Genetic expression and activity of non-pectolytic enzymes and involvement in apple physical properties. 2002: CIRAD/COGENT (France): Improving coconut production in Mozambique through germplasm characterization and evaluation of promising hybrids. Project canceled in 2003 due to lack of funding JAN DEC 1999: PRAXIS/3./3.2/HORT/2163/95: Biomolecular characterisation of Portuguese cultivars of apple and pear. 2

3 JAN DEC 1999: PRAXIS/3/3.2/HORT/2143/95: Sanitation and breeding of Portuguese fruit tree cultivars using a biotechnological approach. JAN DEC 1999: PRAXIS/3/3.2/HORT/2136/95: Study of the biology and sexual reproduction in the chestnut and almond trees: Genetic and fruit breeding. 1996: PAMAF 3088: Study of annual pastures under different soil conditions: annual alfalfas and subclovers. SUPERVISING EXPERIENCE PhD DISSERTATIONS: SINCE 09/2009 João Carlos Martins Fernandes. Thesis: Mineral stress response of Vitis cell wall transcriptome: Identification, characterization and expression of genes from key families involved in wall biosynthesis and modification (Co-orientation with Prof. Sara Amâncio (ISA/UTL)) TEACHING SINCE 2007 Two lab classes / year of Botany II of the Degree in Biology; 3 rd semester (Instituto Superior de Agronomia, Lisbon) 2004 One lecture in Plant Development classes of the Degree in Agronomic Engineering; 9 th semester (Instituto Superior de Agronomia, Lisbon) 2001/2004 Assistant in lab classes of Plant Biotechnology of the Degree in Agronomic Engineering; 6 th semester (Instituto Superior de Agronomia, Lisbon) ORGANIZATION OF SCIENTIFIC MEETINGS Member of the Local Organizing Committee of the "PEROXIDASE 2006 CONGRESS", 6-9 July, Aveiro, Portugal. PEER REVIEWER IN SCIENTIFIC JOURNALS Scientia Horticulturae - 41 manuscripts HortScience - 2 manuscripts Functional Plant Biology 1 manuscript Phytochemistry - 1 manuscript Journal of Food Engineering 1 manuscript J. American Society of Horticultural Sciences - 1 manuscript J. Tropical Forest Science - 1 manuscript PRIZES AND AWARDS 1998 Prize D. Dinis in Agronomic Sciences. Granted by the Prof. Agostinho da Silva Fundation / Montepio Geral Bank 3

4 GRANTS AND FELLOWSHIPS NOV 2006 JUN 2009: Post-Doctoral Grant SFRH/BPD/17565/ FCT JUL 2004 MAR 2005: Researcher AGRO 8.1 (AGRO 518) JUL 2000 JUN 2004: PhD Student SFRH/BD/1133/2000 FCT JAN 1997 DEC 1999: Young Graduate Researcher SFRH/BIC/ FCT PUBLICATIONS: THESIS: Goulão LF (2005). Identification, cdna cloning and expression, and enzymatic activity of proteins involved in ripening and softening of apple (Malus x domestica Borkh. cv Mondial Gala ) fruits. PhD Thesis. Instituto Superior de Agronomia, Lisbon, 269 pp. Goulão LF (1996). Caracterização de germoplasma de Trifolium subterraneum L. Degree in Agronomic Engineering Thesis. Instituto Superior de Agronomia, Lisbon, 87 pp. BOOK CHAPTERS: Goulao LF, Almeida DP, Oliveira CM. "Effect of enzymatic reactions on texture of fruits and vegetables". In: Enzymes in Fruit and Vegetable Processing: Chemistry and Engineering Applications (Ed.: A. Bayindirli). CRC Press, USA. ISBN: (Publication Date: January 15, 2010) PAPERS IN INTERNATIONAL SCIENTIFIC PERIODICALS WITH REFEREE: Goulao LF, Cosgrove DJ and Oliveira CM (2008). Cloning, characterisation and expression analyses of cdna clones encoding cell wall-modifying enzymes isolated from ripe apples. Postharvest Biology and Technology 48: Goulao LF and Oliveira CM (2008). Cell wall modifications during fruit ripening: when a fruit is not the fruit. Trends in Food Science & Technology 19: review article. Goulao LF, Santos J, Sousa I and Oliveira CM (2007). Patterns of enzymatic activity of cell wall-modifying enzymes during growth and ripening of apples. Postharvest Biology and Technology 43: Goulao LF and Oliveira CM (2007). Molecular identification of novel differentially expressed mrnas up-regulated during ripening of apples. Plant Science 172: Carvalho L, Goulão L, Oliveira C, Gonçalves J and Amâncio S (2004). RAPD assessment for identification of clonal identity and genetic stability of in vitro propagated chestnut hybrids. Plant Cell Tissue and Organ Culture 77: Goulão L and Oliveira C (2001). Molecular characterisation of cultivars of apple (Malus x domestica Borkh.) using microsatellite (SSR and ISSR) markers. Euphytica 122: Goulão L, Cabrita L, Oliveira C and Leitão J (2001). Comparing RAPD and AFLP markers in discrimination and estimation of genetic similarities in apple (Malus x domestica Borkh.) cultivars. Euphytica 119: Monte-Corvo L, Goulão L and Oliveira C (2001). ISSR analysis of cultivars of pear and suitability of molecular markers for clone discrimination. Journal of the American Society for Horticultural Sciences 126:

5 Goulão L, Valdiviesso T, Santana C and Oliveira C (2001). Comparison between phenetic characterisation using RAPD and ISSR markers, and phenotypic data of cultivated chestnut (Castanea sativa Mill.). Genetic Resources and Crop Evolution 48: Goulão L, Monte-Corvo L and Oliveira C (2001). Phenetic characterization of plum cultivars by high multiplex ratio markers: Amplified Fragment Length Polymorphisms and Inter- Simple Sequence Repeats. Journal of the American Society for Horticultural Sciences 126: Oliveira C, Mota M, Monte-Corvo L, Goulão L and Silva D (1999). Molecular typing of Pyrus based on RAPD markers. Scientia Horticulturae 79: PAPERS IN NATIONAL SCIENTIFIC PERIODICALS WITH REFEREE: Goulão L, Monte-Corvo L, Mota M, Silva D and Oliveira C (1999). Caracterização de cultivares de macieira (Malus pumila Mill.) e de pereiras (Pyrus spp.) por amplificação aleatória de DNA (RAPD). Revista de Ciências Agrárias XXII(2): Farinha N, Brito A, Goulão L and Carneiro J (1999). Avaliação de linhas avançadas de Trifolium subterraneum L. em selecção nas condições de Elvas. Pastagens e Forragens 20: Goulão L, Farinha N, Tavares-de-Sousa M and Martins J (1999). Caracterização morfológica de linhas de Trifolium subterraneum L. com base em análise multivariada. Pastagens e Forragens 20: PAPERS IN CONFERENCE PROCEEDINGS: Goulão LF and Oliveira CM (2005). Patterns of temporal mrna expression and enzyme activity of pectolytic and non-pectolytic cell-wall degrading enzymes in growth and ripening of apple fruits. Acta Horticulturae 682: Neto C, Goulão L, Clemente J, Gomes MH, Duarte P, Almeida D and Oliveira C (2005). Efeito do 1-metilciclopropeno em maçãs e pêras armazenadas em atmosfera normal. Actas Portuguesas de Horticultura 8(4): Goulão L and Oliveira CM (2004). Identification, genetic expression and enzymatic activity of ripening and softening related proteins in apple fruits. Maturação e Pós-Colheita: Frutos e Hortícolas (Ed.: MG Barreiro), INIAP/EAN. pp Monte-Corvo L, Goulão L and Oliveira C (2002). Discrimination of pear cultivars with RAPD, AFLP, and ISSR. Acta Horticulturae 596: COMMUNICATIONS: ORAL COMMUNICATIONS: Dionísio L, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I, Oliveira CM (2006). Efeito do 1-metilciclopropeno em maçãs Royal Gala armazenadas em atmosfera normal em dois anos consecutivos de ensaio. I Simpósio Nacional de Fruticultura: Programa e Resumos das Comunicações, October 2006, Alcobaça, Portugal. pp.49 [Presented by C. Neto] Goulão L and Oliveira CM (2004). Identification, genetic expression and enzymatic activity of ripening and softening related proteins in apple fruits. IV Iberian Symposium on Maturation and Postharvest 2004, 6-9 October 2004, Oeiras, Portugal. pp [Presented by L. Goulão] 5

6 Goulão L and Oliveira CM (2004). Patterns of temporal mrna expression and enzyme activity of pectolytic and non-pectolytic cell-wall degrading enzymes in growth and ripening of apple fruits. 5th International Postharvest Symposium, 6-11 June 2004, Verona, Italy. pp. 6 [Presented by L. Goulão] Goulão L, Monte-Corvo L and Oliveira C (1999). Identification of European and Japanese plum cultivars based on ISSR (Inter-Simple Sequence Repeats) markers. Resumos do 2º Workshop de Biotecnologia de Plantas, 6-7 May 1999, Faro, Portugal. pp.21 [Presented by L. Goulão] Farinha N, Carneiro J, Brito A and Goulão L (1998). Avaliação de linhas avançadas de Trifolium subterraneum L. em selecção nas condições de Elvas. Programa e Resumos da XIX Reunião de Primavera da Sociedade Portuguesa de Pastagens e Forragens, May, Castelo Branco, Portugal. pp.12. [Presented by N. Farinha] POSTERS IN CONFERENCES: Goulao LF, Vatulescu AD, Ribeiro A, Ramalho JC, Jackson PA (2008). Developing a high troughput method to identify class III and ascorbate peroxidases of coffee differentially expressed under conditions of stress. 8th International Peroxidase Symposium, August 2008, Tampere, Finland. Goulao LF, Vieira-da-Silva S, Jackson PA (2007). Identification of hemicellulose- and pectinmodifying genes expressed during Eucalyptus wood formation. XIth Cell Wall Meeting, August, Copenhagen, Denmark. Physiologia Plantarum 130(4) Abstract 230. Goulao LF, Vieira-da-Silva S, Jackson PA (2007). Cell wall modifications during xylogenesis in Eucalyptus globulus involves divergent transcription of several members from candidate families. IUFRO Tree Biotechnology 2007, 3-8 June, Ponta Delgada - Azores, Portugal. pp.svi.33 Alpalhão A, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I, Oliveira CM (2006). Efeito do 1-metilciclopropeno em pêra Rocha com diferentes estados de maturação armazenadas em atmosfera normal. I Simpósio Nacional de Fruticultura: Programa e Resumos das Comunicações, October 2006, Alcobaça, Portugal. pp.61 Goulao LF, Vatulescu A, Jackson PA (2006). Two peroxidases from eucalyptus wood with role in cellular growth. Peroxidase 2006: Abstracts & Programme, 6-9 July 2006, Aveiro, Portugal. pp.46 Neto C, Goulão L, Clemente J, Gomes MH, Duarte P, Almeida D and Oliveira C (2005). Efeito do 1-metilciclopropeno em maçãs e pêras armazenadas em atmosfera normal. V Congresso Ibérico de Ciências Hortícolas/IV Congresso Iberoamericano de Ciências Hortícolas, May 2005, Porto, Portugal. pp Goulão L and Oliveira CM (2002). Cloning and characterization of a cdna clone encoding an expansin isoform with expression in ripening apples. Programa e Resumos do XIII Congresso Nacional de Bioquímica, 5-7 December 2002, Lisboa, Portugal. pp.57 Goulão L and Oliveira, CM (2002). Evidence of the presence of several isoforms of endo-beta- 1,4-glucanases, xyloglucan endotransglycosylase and expansins in over-ripe apples. 13th Congress of the Federation of European Societies of Plant Physiology: Book of Abstracts, 2-6 September 2002, Hersonissos - Crete, Greece. pp.338 Monte-Corvo L, Goulão L and Oliveira C (2000). Comparison of molecular markers (RAPD, AFLP, ISSR and SSR) on pear (Pyrus communis L.). VIII International Symposium on Pear Growing (ISHS), 4-9 September 2000, Ferrara-Bologna, Italy. pp

7 Goulão L, Farinha N, Sousa M and Martins J (1999). Caracterização morfológica de linhas de Trifolium subterraneum L. com base em análise multivariada. Programa e Resumos da XX Reunião de Primavera da Sociedade Portuguesa de Pastagens e Forragens - Pastagens e Forragens em Pecuária Extensiva, April 1999, Elvas, Portugal. pp.28 Goulão L, Cabrita L, Oliveira C and Leitão J (1998). Characterisation of apple (Malus x domestica Borkh.) cultivars by AFLP markers. Programa e Resumos do XI Congresso Nacional de Bioquímica, November, Tomar, Portugal. pp.138 Monte-Corvo L, Mota M, Goulão L, Silva D and Oliveira C (1998). Taxonomic use of RAPD markers in Pyrus. Abstracts of the XXV International Horticultural Congress (IHC), 2-7 August 1998, Brussels, Belgium. pp.445 OTHER PUBLICATIONS: DIVULGATION PAPERS: Oliveira C, Goulão L, Monte-Corvo L, Mota M, Nolasco G, Leitão J, Silva D and Sousa R (2000). Caracterização biomolecular de cultivares portuguesas de macieira e pereira: Defender o património genético. Investigação Agrária 1: Goulão L, Hilário L, Fortunato M and Nunes P (1998). A importância dos bancos de germoplasma na manutenção da biodiversidade. Agros 81: GENBANK (NCBI) SUBMISSIONS: 59 SEQUENCES (Corenucleotide) EUCALYPTUS CELL WALL-MODIFYING ENZYMES INVOLVED IN WOOD FORMATION: DQ DQ100342, DQ DQ376138, DQ DQ APPLE RIPENING-RELATED CELL WALL-MODIFYING ENZYMES: AF527800, AY144593, AY144594, AY309436, AY350734, AY376878, AY TRANSCRIPTS DIFFERENTIALLY EXPRESSED DURING APPLE RIPENING: AY AY SCIENTIFIC ASSOCIATION MEMBERSHIP: International Society for Horticultural Science Portuguese Biochemical Society / Portuguese Society of Plant Physiology SCIENCE AND SOCIETY ACTIVITIES: 2009 Participation in the Eco-Bio/IICT Semana da Ciência - Ciência Viva program (Short conference: Molecular Detectives) 2009 Participation in the Café Científico initiative of the European Researchers Night (Informal talk: Molecular Agriculture: look at the genes to improve the production) 2005 and 2008 Participation in the ITQB s Open Day Plant Division Stands updated: November