Validation of the Paratest as efficient method for parasitological diagnosis TEODORO B. K.; ROBERTO T. N.; BRASIL D. M. E SOUZA L. B.; SOUZA M. C.; PAULETTO M. C. A. C.; MAMED J. A.; SBRAVATE-MARTINS C. A. Keywords: Diagnosis, Parasitosis, Paratest INTRODUCTION With the phrase Jeca [yokel] is not this: he is like this, the writer Monteiro Lobato, already in the 30s, gave us the privilege of his understanding of the problem of our rural endemics, describing our man of the country not as a individual who is lazy by nature but rather, in reality, a chronically parasitized person. A new century reemerges and the problem of parasitosis persists in our country as an important issue of Public Health. Intestinal parasitosis affect mainly the physical and mental development of children, who are the most vulnerable and transmission and maintenance in the population is the result of an interactive processes between the agent, environment and human host. It is possible to identify the agent through parasitological test of the feces, researching different parasitic forms eliminated in the feces, through various, already standardized methodologies. Today, there are countless applicable methods, aiming at facilitating the processing and identification of the sample. PURPOSE Since the Methodology is of fundamental importance, the purpose of this work was to evaluate the Paratest method before two other methods very known and conducted in our circles: the Hoffman and Ritchie methods.
METHODOLOGY A total of 165 samples were evaluated, obtained from a population made up mainly of children from Capivari Region in State of São Paulo, Brazil. These samples were subjected to three methods: Hoffman, Ritchie and Paratest. RESULTS According to Graph 1, the results determined to date show a percentage of approximately 68.5% of positive, where prevalence was observed of 20.5% of Ascaris lumbricoides, 15% of Trichuris trichiura, 5.3% of Strongyloides stercoralis, 5.3% of Enterobius vermicularis, 1.7% of Hymenolepis nana and 0.9% of Ancylostoma sp. Among protozoa, the highest prevalence was of Endolimax nana (77%), followed by Entamoeba coli (32.7%), Giardia lamblia (29.2%) and Blastocystis hominis (5.3%). The preliminary results reveal greater efficiency of the Paratest method since, in relation to the other methodologies, it has 91.1% of positivity, while the Hoffman method had 88.3% and the Ritchie method had 85.5% (Figure 2).
Graph 1 - Parasitic Incidence in the Total Positive Samples Parasite Species
Figure 2 Percentage of positive samples by the Paratest compared to other Methods CONCLUSION Other studies are being evaluated to validate the method, but our results show a greater efficiency of PARATEST in relation to other classical methods in parasitological diagnosis.
Data of 1) Scientific Investigation 2) Poster 3) Biological Sciences 4) UMESP [Universidade Metodista de São Paulo] 5) Validation of the Paratest as efficient method for parasitological diagnosis 6) Professor Doctor MARTA CRISTINA SOUZA; Professor Masters MEIRE CASTRO ALVES PAULETTO; Professor JONATAN ALE MAMED; Doctor CLIZETE APARECIDA SBRAVATE MARTINS. 7) Marta.souza@metodista.br 8) BRUNO KASHIBA TEODORO; CLIZETE APARECIDA SBRAVATE MARTINS; DAYANE MARTINS BRASIL; JONATAN ALE MAMED; LETÍCIA BONATO; MARTA CRISTINA SOUZA; MEIRE CASTRO ALVES PAULETTO; THIAGO ROBERTO NUNES; 9) 138625, 92, 10) Dayane Martins Brasil 11) Rua Veja, 357 Jardim Inamar, Diadema SP, Brazil, Cep [zip code]:09973-020 12)bruno.kashiba@bol.com.br, iaprobio@.com.br, day.brasil@yahoo.com.br, jonatan_mamed@uol.com.br, leticiabonato@hotmail.com, marta.souza@metodista.br, meire.pauleto@metodista.br, tnroberto@hotmail.com, 13) 11 8998-5358 /4059-2453 14) Ok 15) NEVES, David Pereira; MELO A L.; GENARO O. E LINARDI P. M. Parasitologia Humana. : Atheneu, 2000. 428 pgs.