Humberto de Mello Brandão

Transcrição

1 Humberto de Mello Brandão I JORNADAS INTERNACIONALES DE NANOTECNOLOGÍA en AGROINDUSTRIAS y AGROALIMENTOS humberto.brandao@embrapa.br +55 (32) San Martin, 4 de Noviembre 2014

2 Mission Provide feasible solutions for the sustainable development of the Brazilian agribusiness through knowledge and technology generation and transfer. 2

3 AGRONANO NETWORK Coordination: Embrapa Instrumentação 158 researchers / 53 Institutions

4 National Nanotechnology Laboratory for Agribusiness

5 Management Component Project 1 (CP1) CP2:Sensors CP3:Films CP4:Composites CP5:Materials CP6:Security CP7:T.T. Participants (16) CNPDIA CNPMA CENARGEN CNPAT CNPSo CNPA CNPGL CPPSE CNPGC UNB UNIFESP UFMS CEETPS UNESP UFSCar USP Participants (8) CNPDIA CENARGEN CNPAT CPATU CTAA UFRJ UNAERP USP Participants (12) CNPDIA CNPAT CPAP CTAA CNPF CNPA UFRJ UECE UFSCar UFPB UNESP UFRN Participants (24) CNPDIA / CNPGL CPPSE / CENARGEN CPATU / CNPA CNPGC UFPr / UFMG USP / SENAI UFPB / FURB UNICAMP UFSCar UEPG / UFVJM UFPe / UNESP UFG IAC AOSA UFPA UFOP Participants (17) CNPDIA CNPGL CPPSE CNPMA CNPAT CNPA CPATU CENARGEN UFJF UFMG UFSCar EPAMIG CEFET-Pr UNESP USP UFRA UFPa Participants (9) CNPDIA CENARGEN CNPAT CNPSo CNPGL CTAA DTT AIT USP

6 Management Committee Component Project 1: Management Leader: Cauê de Oliveira (CNPDIA) co-leader: Odilio Assis (CNPDIA) Component Project 2: Sensors and Biosensors Leader: Fernando Fonseca (Poli-USP) co-leader: João Naime (CNPDIA) Component Project 3: Ultra-thin edible film and active packaging Leader: Rubens Bernardes Filho (CNPDIA) co-leader: Lucimara Forato (CNPDIA) Component Project 4: Bionanocomposites Leader: Morsyleide Rosa (CNPAT) co-leader: José Marconcini (CNPDIA)

7 Management Committee Component Project 5: New uses of nanomaterials in agriculture Leader: Cauê de Oliveira (CNPDIA) co-leader: Humberto de Mello Brandão (CNPGL) Component Project 6: Impact Assessment in Nanotechnology (Security) Leader: Humberto de Mello Brandão (CNPGL) co-leader: Luíz Mattoso (CNPDIA)/Leonardo Fraceto (UNESP) Component Project 7: Technology Transfer Leader: Rubens Bernardes Filho (CNPDIA) co-leader: Lucimara Forato (CNPDIA)

8 WORKSHOPS: AGRONANO NETWORK 49 expanded abstracts 87 expanded abstracts 206 expanded abstracts * 166 expanded abstracts 192 expanded abstracts * AGRONANO NETWORK & FAO cnpdia.redeagronano@embrapa.br

9 Get to know Embrapa Dairy Cattle The National Dairy Cattle Research Center

10 Our Team Category Embrapa 2012 Embrapa Dairy Cattle 2013 Researcher 2, (16) Research Analyst 2, (02) Support Assistants 4, TOTAL 9,

11 Arrangement: Optimization of Dairy Production PROJECTS Genetic evaluation and selection in dairy cattle herds. Genomics applied to dairy Precision animal husbandry and metrics applied to bioefficiency Nanotechnology applied to animal health. Tick control strategies Methods for generating transgenic ruminants. In vivo and in vitro systems for embryo production and cryopreservation Dairy farm waste management and utilization

12 Arrangement: Milk Quality PROJECTS Microorganisms of interest for the agroindustry and livestock production Temporal and spatial monitoring of indicators of milk quality Thermal stability of milk. Fraud detection Influence of animal nutrition on the production and composition of milk Isolation, identification and control of pathogens in milk and dairy products. Monitoring of antimicrobial resistance in bacteria pathogenic to dairy cattle Nanotechnology

13 Laboratories 1.Animal Health Protection 2.Animal Reproduction 3.Bioinformatics and Animal Genomics 4.Chromatography 5.Entomology 6.Feed Analysis 7.Milk Microbiology 8.Milk Quality 9.Molecular Genetics 10.Nanotechnology 11.Parasitology 12.Plant Biotechnology and Physiology 13.Plant Genetics 14.Rumen Microbiology 15.Soil, Water and Plant

14 Infrastructure: lab. Nano

15 Infrastructure: lab. Nano

16 Experimental Stations JoséHenrique Bruschi (CE-JHB): ha, ruminants, with pastures, forage and milk production, laboratories and training facilities for farmers and technicians. Santa Mônica(CESM): 1,678 ha, with availability of areas for pasture and forage production, bovines (1200)and laboratories, and infrastructure for field research work.

17 Multiuser Complex for Bioefficiency and Sustainability of Livestock Buildings: 22 units Total area: 13,700 m2 Lodging capacity: 400 animals between large and small ruminants

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19 RFID Data control TAG - Identification Load cell- weighing of food

20 Consumo de água e pesagem dos animais a pasto

21 Detection of mastitis

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25 Credits: Embrapa Instrumentação Agropecuária (Dr. Odílio B. G. Assis) Nano s em alimentos

26 Nano/Foods Chitosan CMC Zeins Credits: Embrapa Instrumentação Agropecuária (Dr. Odílio B. G. Assis)

27 Credits: Embrapa Instrumentação Agropecuária Electronic Tongue

28 Figura: A) sensor (microeletrodo interdigitado); B) suporte com sensores. Credits: Embrapa Instrumentação Agropecuária

29 Deposition of Films Credits: Embrapa Instrumentação Agropecuária Adaptado de Ribeiro (2007)

30 Credits: Embrapa Instrumentação Agropecuária Identification of orange juice

31 DE PAULA JUNIOR, A. 2009

32 3 2 1 Escore no PC Escore no PC1 Adulterated milk: whey protein

33 Controlled release of P4

34 DNA Delivery: transgenic embryo Bioreactor: therapeutic proteins Brandão et al. in press Vanroose et al. 2000

35 Transgenic mammalian embryo Brandão et al. in press

36 DNA Delivery Pereira et al. submitted paper

37 Mastitis Control

38 Dry cow period PMN Apoptosis Increased intraluminal pressure h

39 Oil based Nano

40 S. aureus Capilar sanguíneo Tecido glandular Célula muscular Cisterna glândula Células epiteliais Alvéolo Ducto do teto Nanoparticle Araujo et al,

41 Castanheira et al

42 Phase II clinical trial: 7157 Araujo et al

43 Cabra com A. pyogenes

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45 Keratoconjunctivitis treatment Cationic nanoparticles Mucins N= 58 cows

46 Keratoconjunctivitis: Phase II clinical trial Control Oil based x 4,28 Nano x 9,64 * (P<0,05) N= 46 cows

47 Organic Dairy Production: Nanopropolis lantimicrobial activity lanti-inflammatory activity Silva et al.;2014

48 Organic Dairy Production: Nanopropolis

49 Phase I clinical trial 2014

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52 Ruminal controlled-release

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58 Cooperation with INTA Rafaela Approved project Characterization of virulence factors and nanostructuring of immunogens of Staphylococcus aureus for bovine mastitis control ~US$ 150, (CNPq) and ~US$ 120, (Embrapa) Humberto de Mello Brandão & Luis Fernando Calvinho

59 Collaborators CNPGL- Humberto M. Brandão, Juliana Gern, Carla C. Lage, Maria Aparecida V. Paiva Brito, José R. Brito, Édna F. Arcuri, Juliana A. Leite, Marta F.M. Guimarães, Wanderlei F. Sá, Cládio Nápolis, Guilherme N. Souza, Eliane Vargas, Saulo R. da Silva, Juliana Gern; Nívea; Patrícia Vilhena; Luiz Sergio; Alessandro de Sá CNPDIA- Luiz H. C. Mattoso, Cauê Ribeiro, José Manoel Marconcini UFOP Vanessa C. F. Mosqueira, Raquel UFJF - Nádia R. Barbosa; Luiz Fernando Cappa UFSC Bianca Zen; Elenara Lemos UFMG Valbert Cardoso Nascimento CPPSE- Ana Carolina de Souza Chagas USP/POLI- Fernando J. Fonseca, Leonardo G. Paterno, Guilherme Braga, Nadja Wiziack, Camila Gregorut USP/VRA- Ed H. Madureira, Annelise S. Traldi Unesp- Helio Langoni, Marcella INTA- Luis Fernando Calvinho Paris 7 Roberta Brayner