Full Name: Luís Filipe Sanches Goulão

Transcrição

1 CURRICULUM VITAE LUIS F.S. GOULÃO PERSONAL DATA Full Name: Luís Filipe Sanches Goulão Nationality: Portuguese Birth Place: Carnaxide, Oeiras Date of Birth: July, 1973 Institutional Address: Tropical Research Institute [Instituto de Investigação Científica Tropical IICT, IP] BioTrop-Agri4Safe, Pólo Mendes Ferrão Pavilhão de Agro-Indústrias e Agronomia Tropical (3º piso) Tapada da Ajuda, Lisboa PORTUGAL Phone: goulao@iict.pt EDUCATION PhD IN AGRONOMIC ENGINEERING Postharvest Physiology and Molecular Biology of Fruits TECHNICAL UNIVERSITY OF LISBON (SCHOOL OF AGRONOMY; ISA/UTL), LISBON - PORTUGAL MSci IN SUSTAINABLE AGRICULTURE AND HORTICULTURE (Scholar Part) TECHNICAL UNIVERSITY OF LISBON (SCHOOL OF AGRONOMY; ISA/UTL), LISBON PORTUGAL DEGREE IN AGRONOMIC ENGINEERING Plant Breeding TECHNICAL UNIVERSITY OF LISBON (SCHOOL OF AGRONOMY; ISA/UTL), LISBON PORTUGAL RESEARCH INTERESTS - Agricultural RESEARCH FOR DEVELOPMENT (AR4D) and innovation in AGRIbusiness - FOOD AND NUTRITIONAL SECURITY AND SAFETY (hunger and "hidden hunger") - COLLABORATIVE RESEARCH with CPLP countries EXPERTISE: PLANT SCIENCES APPLIED TO ECONOMICALLY IMPORTANT CULTIVATED SPECIES - Postharvest biology and technology: fruit ripening, quality and storage - Domestication and exploitation of native tropical fruits and vegetables towards food and nutritional security - Molecular, biochemical and enzymatic modifications of the plant cell wall with agronomic/industrial relevance - Applications of molecular markers in plant management, breeding and agriculture - Identification, expression and functional characterization of genes and metabolites (-OMICs) with agronomic and industrial relevance in economically important plant species TRAINING IN INTERNATIONAL LABORATORIES Visiting scholar at the Department of Genetics - University of Georgia, Athens, GA - USA (supervision by Prof. Chung-Jui Tsai and Dr. Scott Harding), September-November 2004 Visiting scholar at the Department of Biology - Pennsylvania State University, PA USA (supervision by Prof. Daniel Cosgrove), March-June 1

2 ADVANCED TRAINING 2012 European Cooperation in Science and Technology (COST) Course: "Next Generation Sequencing Data Analysis with Chipster, June, Helsinki, Finland 2010 The Gulbenkian Training Programme in Bioinformatics Course: Automatic Annotation and Data Mining, May, Oeiras, Portugal 2009 The Gulbenkian Training Programme in Bioinformatics Course: Workflows and Programatically Accessible Tools, November, Oeiras, Portugal 2009 The Gulbenkian Training Programme in Bioinformatics Course: Microarray Data Analysis using R and Bioconductor, September, Oeiras, Portugal 2008 European Science Foundation for Functional Genomics (ESFFG) Workshop: "Advanced large scale gene expression profiling focus on mirnas, ChIP-on-chip and whole genome sequencing", May, Turku, Finland 2002 Workshop "Coconut genetic resources management using microsatellite kit and dedicated statistical software", April, Montpellier, France SCIENTIFIC APPOINTMENTS SINCE JUL 2009: Associate Researcher ( Investigador Auxiliar ) at the Tropical Research Institute (IICT), Oeiras. Head of the Agri4Safe Group, Lisbon, since Oct 2012 NOV JUN 2009: Post-Doctoral Fellow at the Plant Cell Wall Group Instituto de Tecnologia Química e Biológica (ITQB/UNL), Oeiras JAN OCT 2006: Graduate Student at the Plant Cell Wall Group - Instituto de Tecnologia Química e Biológica (ITQB), Oeiras JUL MAR 2005: Research Grantee for Program AGRO 8.1 at the Technical University of Lisbon (School of Agronomy; ISA/UTL), Lisbon / Painho Fruit Storage Central, Cadaval JUL JUL 2004: PhD Student at the Horticulture Department - Technical University of Lisbon (School of Agronomy; ISA/UTL), Lisbon JAN DEC 1999: Young Graduate Researcher Fellow at the Technical University of Lisbon (School of Agronomy; ISA/UTL), Lisbon / University of Algarve (Faculty of Engineering and Natural Resources), Faro / National Institute for Fruit Research Vieira Natividade (ENFVN, IP), Alcobaça MAR DEC 1996: Trainee at the Department of Forage Crops - National Institute for Plant Breeding (ENMP, IP), Elvas MEMBER OF EXPERT GROUPS AND TASK FORCES SINCE APR 2014 High Level Policy Dialogue Expert Working Group on Science, Technology and Innovation for Food and Nutrition Security and Sustainable Agriculture in EU-Africa cooperation, European Commission SINCE JAN 2013 IICT Representation in the Secretariado do Grupo de Trabalho do Ensino Superior e de Instituições de Investigação (GT-ESII) to support the implementation of the CPLP s Food and Nutritional Security Strategies PARTICIPATION IN FINANCED RESEARCH PROJECTS SINCE JUN 2014: Fundo Nacional de Investigação (FNI) de Moçambique: Avaliação frutícola e nutricional e potencial de conservação de frutos nativos para valorização e segurança nutricional. [Project Coordinator at IICT] SINCE JUN 2014: Fundo Nacional de Investigação (FNI) de Moçambique: Promoção da valorização de espécies da floresta de miombo moçambicano no contexto do bio-empreendedorismo. SINCE FEB 2014: German Federal Enterprise for International Cooperation (GIZ): Comparing two fire management strategies in the Limpopo Tourism and Parks: creating the foundations for a fire management system in the Limpopo National Park. SINCE JUL 2013: PTDC/AGR-PRO/3386/2012: Implications of environmental changes of CO2 and temperature at the plant and bean quality levels in the coffee tree (Coffea spp.) - multidisciplinary characterization of the impact and acclimation ability of the producing species. SINCE JUL 2013: IFAP - ProDeR PA nº 46511: RTDT Competitividade Internacional Rede de Tratamento e Difusão de Tecnologia com vista à Competitividade Internacional do Tecido Empresarial Nacional. SINCE FEB 2013: International Rice Research Institute (IRRI): FrutosNativos4SAN Mozambique [Program Coordinator] 2

3 SINCE JAN 2013: Camões, Instituto da Cooperação e da Língua (CICL): Formação Avançada de Recursos Humanos na Área de Biotecnologia aplicada à gestão e monitorização de recursos naturais Fase II. SINCE JAN 2013: Fundo Aberto Universidade Eduardo Mondlane (Fundo de Investigação Científica; FIC): Análise ecológica e molecular para avaliação do estado de conservação da população de chanfuta (Afzelia quanzensis Welw.) da floresta de Michafutene (Marracuene, Maputo). SINCE JAN 2013: Fundo Aberto Universidade Eduardo Mondlane (Fundo de Investigação Científica; FIC): Mapeamento e diversidade genética de Hypoxis spp. (batata africana) e Warburgia salutaris (chibaha) na província de Maputo. SINCE MAR 2012: PTDC/AGR-GPL/116923/2010: VitiShade - Abscission of seedless grapes: shedding the genes from the shade. [Project Coordinator] SINCE JAN 2012: PTDC/AGR-ALI/118477/2010: SafeFruit - Aureobasidium pullulans, a biofungicide for the control of postharvest decay in pears: insights into its action mechanisms. SINCE JAN 2012: IFAP - ProDeR PA nº 45261, partnership #449: AROMA4SAFE [Project Coordinator at IICT] SINCE JAN 2012: IFAP - ProDeR PA nº 23923, partnership #373: PROdUVA [Project Coordinator at IICT] JAN 2011 DEC 2012: PUBLIC-PRIVATE PARTNERSHIP IICT/GEOCAPITAL HOLDINGS (Macau): Sustainable biodiesel production from Jatropha curcas in countries of the CPLP (Portuguese-speaking countries). DEC DEC 2011: BIOVERSITY INTERNATIONAL F06: Bridging the divide between natural resource conservation strategies and the role of key forest genetic resources in Niassa National Reserve, Mozambique. NOV 2007 DEC 2010: PTDC/AGR-AAM/64078/2006: Molecular evaluation and characterization of tolerance mechanisms to adverse environmental conditions in Coffea sp. - central role of the oxidative stress control at leaf level. JAN 2005 JUN 2009: POCTI/AGR/46671/02: Cell wall proteins with roles in xylogenic programmes in eucalyptus. JAN MAR 2005: AGRO 518: Improving postharvest quality and safety of pear, apple and kiwi. JUL JUN 2004: POCTI/33733/99: Genetic expression and activity of non-pectolytic enzymes and involvement in apple physical properties. 2002: CIRAD/COGENT (France): Improving coconut production in Mozambique through germplasm characterization and evaluation of promising hybrids. (Project canceled in 2003 due to cancelling of funding) JAN DEC 1999: PRAXIS/3./3.2/HORT/2163/95: Bio-molecular characterization of Portuguese cultivars of apple and pear. JAN DEC 1999: PRAXIS/3/3.2/HORT/2143/95: Sanitation and breeding of Portuguese fruit tree cultivars using a biotechnological approach. JAN DEC 1999: PRAXIS/3/3.2/HORT/2136/95: Study of the biology and sexual reproduction in the chestnut and almond trees: Genetic and fruit breeding. 1996: PAMAF 3088: Study of annual pastures under different soil conditions: annual alfalfas and sub-clovers. SUPERVISING EXPERIENCE (at graduate level only) PhD THESIS: SINCE JAN 2011 Sara Nobre Gonçalves Domingos. Thesis: Thinning via shading in seedless table grapes: Unravelling the physiological and molecular basis of berries abscission (Co-orientation with Prof. Cristina Moniz Oliveira (ISA/UTL); grant: SFRH/BD/69076/2010) SINCE JAN 2010 João Carlos Martins Fernandes. Thesis: Mineral stress response of Vitis cell wall transcriptome: Identification, characterization and expression of genes from key families involved in wall biosynthesis and modification (Co-orientation with Prof. Sara Amâncio (ISA/UTL); grant: SFRH/BD/64047/2009) MSci THESIS: SINCE DEC 2013 Filipa Neto. Thesis: Fighting food insecurity in Mozambique: nutritional evaluation of a blend of dehydrated native vegetables used as a food supplement (Co-orientation with Prof. Margarida Moldão (ISA/UL - MSci in Food Engineering)) SINCE OCT 2013 Déjan Bogosavljevic. Thesis: Evaluation of different production and drying methodologies in the aromatic quality of Stevia rebaudiana (Co-orientation with Prof. Margarida Moldão (ISA/UL - MSci in Food Engineering)) SINCE SEP 2013 César Castelo Branco Esteves. Thesis: Insights on the mechanisms of anthracnose tolerance in physic nut (Jatropha curcas) (Co-orientation with Prof. Ana Lúcia Leitão (FCT/UNL - MSci in Food Technology and Safety)) SINCE SEP 2013 Maria João Prata. Thesis: Assessment of Rocha pear cell wall modifications as a defense mechanism against postharvest molds, under biocontrol (Co-orientation with Prof. Maria Teresa Rebelo (FC/UL MSci in Human Biology and Environment)) SINCE JUL 2013 Sara Martins Paiva. Thesis: Evaluation of oligosaccharides in fruit postharvest storage potential (Co-orientation with Prof. Ana Lúcia Leitão (FCT/UNL - MSci in Food Technology and Safety)) 3

4 JAN 2012 JUL 2013 Ivete Sandra Maquia. Thesis: Molecular characterization of forest genetic resources from the Miombo woodlands at the Niassa National Reserve: development of barcode- and ISSR- markers, Eduardo Mondlane University, Mozambique (Co-orientation with Dr. Ana Ribeiro (IICT) MSci in Biotechnology) JAN 2012 JUL 2013 Elias Manuel Amadeu Militão. Thesis: Genetic characterization of Afzelia quanzensis Welw. populations from the Michafutene forest, Eduardo Mondlane University, Mozambique (Coorientation with Dr. Ana Ribeiro (IICT) MSci in Biotechnology) CAPACITY BUILDING: JAN 2014 MAY 2014 Burcu Belge (Visiting Scholar; PhD student from the University of Lleida, Spain): Quantification of the expression of cell wall- and cuticle-modifying genes in response to postharvest treatments in peach and cherry APR 2013 MAR 2014 Vânia Alexandra da Silva Cardoso (Researcher; grant associated with the VitiShade project): Searching for molecular signaling of flower/fruitlet abscission in Vitis vinifera JUL 2013 SET 2013 Miguel Fraga (Summer trainee; Student of Biotechnology from the Universidade da Beira Interior): qrt-rt-pcr quantification of MAP(K)n transcription factors and hormone receptors and their effect in regulating floral abscission triggered by different stimuli JUL 2013 SET 2013 Guilherme Tonial Neves (Summer trainee; Student of Biotechnology from the Universidade Federal dos Grandes Dourados - BRAZIL / Universidade da Beira Interior): Identification and expression of genes related with anthracnose tolerance in Jatropha curcas, a biodiesel producing species AUG 2012 DEC 2012 Ana Cláudia Raposo (Researcher; grant associated with the VitiShade project): Agronomic, physiological and molecular assessment of seedless grapes induced for flower/fruitlet abscission under field conditions FEB 2011 JUL 2012 Vanessa Alexandra Victoriano dos Santos (Degree in Biotechnology; Universidade Lusófona de Humanidades e Tecnologias (ULHT)): Molecular characterization of the IICT Jatropha curcas germplasm collection JAN 2011 AUG 2011 Juliana Barbosa (PhD student; Lavras Federal University (UFLA), Brazil): Association of coffee quality with physical and chemical parameters of the green bean DEC 2010 Celestino Uamusse (Researcher, Center for Research and Development in Ethonobotany, Mozambique): "Basic molecular biology and bioinformatics methods" (co-orientation with Dr. Ana Ribeiro and Dr. Ana Fortunato (IICT)) LECTURING In Portugal: : Two lab classes / year (Biophysics of the primary cell wall) in Plant Biology classes of the Degree in Biology; 3 rd semester (ISA/UTL, Lisbon) 2012: Two lectures in Genetic Engineering classes of the Degree in Plant Biotechnology; 3 rd semester (Coimbra Agronomy School, Coimbra) 2011: Lecture The paradoxical characteristics of the primary cell wall in the Workshop Hidden Stories Within Cell Walls (ISA/UTL, Lisbon) : One lab class in Methods of Molecular Analysis classes of the MSci in Functional Biology (ISA/UTL) 2010: One lecture in Modified Organisms classes of the MSci in Biofuels; 1 st semester (Coimbra Agronomy School, Coimbra) 2004: One lecture in Plant Development classes of the Degree in Agronomic Engineering; 9 th semester (ISA/UTL) : Assistant in Plant Biotechnology lab classes of the Degree in Agronomic Engineering; 6 th semester (ISA/UTL) In Portuguese Speaking Countries (CPLP): SINCE 2011: Invited Lecturer at the Eduardo Mondlane University (UEM), Maputo, Mozambique - Master (MSci) in Biotechnology: SINCE 2011: Transcriptomics and Metabolomics: Module CB10: Systems Biology 2014: Measurement of Biodiversity and Genetic Structure - Concepts and Calculations: Module CB07: Genetic Characterization Techniques 2013: Theoretical and practical workshop Quantification of gene expression using quantitative real-time PCR (Centre of Biotechnology (CB) - Eduardo Mondlane University (UEM), Maputo, Mozambique), February. 4

5 EVALUATOR IN INTERNATIONAL PROJECT CALLS 2011 AND French National Research Agency (Agence Nationale de la Recherche; ANR). Committee: "Interface Biology-Physics, Interface Biology-Chemistry and Biotechnology". CONSULTING AND ADVISORY FOR THE PRIVATE SECTOR: OCT-DEC 2013 Consulting for the study of the banana value-chain in Benguela and Baía-Farta, Angola (promoter: World Bank / Fundo de Apoio Social; Programa de Desenvolvimento Local) - Estudo da Cadeia de Valor da Banana nos Municípios de Benguela e Baía Farta - Província de Benguela ; Programa de Desenvolvimento Local; Consultors: Ana Portugal Melo, Carolina Bastos, Cristina Vaz Tomé and Luís Filipe Goulão. 75 pp Scientific advisory on the implementation of a "Coffee Science Centre in Campo Maior (promoter: Grupo Nabeiro) JURY PARTICIPATION IN ACADEMIC DEGREES (at graduate level only) PhD DISSERTATIONS: 2014 Inês Maria Castelhano Graça Andrade. Dissertation: Análise funcional de uma quitinase da classe III (CgCHI3) e de uma glutationa S-transferase (CgGST), envolvidas na simbiose entre Casuarina glauca e Frankia - PhD in Biochemistry (Minors: Biotechnology) FCT/UNL, Costa de Caparica, 4 th July (5 th opponent) José Mário Leitão Ribeiro. Dissertation: The importance of extensin network formation in the definition of primary cell wall properties - PhD in Biological Sciences ITQB/UNL, Oeiras, 29 th January (3 rd opponent). MSci DISSERTATIONS: 2013 Hugo Paulo Vale Nóbrega. Dissertation: Utilização de redes de ensombramento para monda de bagos em uva de mesa - MSci in Agronomy ISA/UL, Lisbon, 27 th December (Main opponent) Lucinda Alculete Lopes de Araújo Feitio. Dissertation: The importance of ubiquitin binding for a novel group for endosomal trafficking protein in Arabidopsis thaliana - MSci in Biotecnology CB/UEM, Maputo, Mozambique, 19 th July (Main opponent) Frederic Cobb. Dissertation: Effect of irrigation on berry and skin cell wall composition in the grape varieties Touriga Nacional and Trincadeira - MSci Vinifera EuroMaster, European Master of Sciences of Viticulture and Oenology ISA/UTL, Lisbon, 4 th December (Opponent) Carlos André Silva Correia. Dissertation: Flower and berry thinning by shading in Sugraone and Crimson Seedless table grapes - MSci in Agronomy ISA/UTL, Lisbon, 16 th March (Main opponent). ORGANIZATION OF SCIENTIFIC MEETINGS 2014 Co-coordinator and Member of the Organizing and Scientific Committees of the: 1 st NATIONAL WORKSHOP ON NATIVE FRUIT CROPS to be held on November, Maputo, Mozambique Member of the Organizing Committee of the: MINERAL IMPROVED CROP PRODUCTION FOR HEALTHY FOOD AND FEED: ENHANCED NUTRITIONAL VALUE OF PLANT-DERIVED FOOD OR FEED (3 rd Annual Workshop - COST Action FA0905. All WGS+MC Meeting), October, Lisbon, Portugal Member of the Scientific Committee of the "MOZAMBIQUE INTERNATIONAL CONGRESS: HISTORY, MEMORY AND SCIENCE, October, Lisbon, Portugal Member of the Local Organizing Committee of the "PEROXIDASE 2006 CONGRESS", 6-9 July, Aveiro, Portugal. EDITORIAL ACTIVITY IN SCIENTIFIC JOURNALS MEMBER OF THE EDITORIAL BOARD: SINCE 2012: International Scholarly Research Notices, Hindawi Publishing Corporation (termed ISRN Agronomy, International Scholarly Research Network until 2014). 5

6 : International Journal of Plant Physiology and Biochemistry (IJPPB), Academic Journals. PEER REVIEWER: 108 manuscripts (MS) to date: Scientia Horticulturae - 49 MS; Food Control 9 MS; Journal of Agricultural and Food Chemistry 5 MS; HortScience 2 MS; Carbohydrate Polymers 5 MS; PLOS ONE 2 MS; Plant Cell Tissue and Organ Culture 2 MS; Tree Physiology 2 MS; Functional Plant Biology 1 MS; Phytochemistry 1 MS; Industrial & Engineering Chemical Research 1 MS; Journal of Food Engineering 1 MS; Journal of the American Society of Horticultural Sciences - 1 MS; Journal of Horticultural Science & Biotechnology 1 MS; Sustainability 1 MS; Journal of Biotechnology 1 MS; Industrial Crops and Products 2 MS; Plant Systematics and Evolution 1 MS; The Scientific World Journal: Agronomy 3 MS; ISRN Agronomy 11 MS; African Journal of Biotechnology 1 MS; Journal of Biological and Food Science Research 1 MS; International Journal of Food, Agriculture and Environment 1 MS; International Journal of Plant Physiology and Biochemistry 1 MS; Emirates Journal of Food and Agriculture 1 MS; African Journal of Agricultural Research 1 MS; Universitas Scientiarum 1 MS; Journal of Tropical Forest Science 1 MS SCIENTIFIC ASSOCIATIONS MEMBERSHIP: International Society for Horticultural Science (ISHS; member no ) Portuguese Biochemical Society / Portuguese Society of Plant Physiology (SPB; member no. 3274) Portuguese Society of Horticulture (Associação Portuguesa de Horticultura - APH; member no Secretary of the Directive Board since May 2013) Professional networks: International Tropical Fruits Network (TFNet) The Postharvest Education Foundation (TEF) IdCPLP: Rede de Investigação e Desenvolvimento na CPLP Plataforma de Biotecnologia de Plantas da CPLP Liga de Amigos do Jardim Botânico Tropical (LAJBT; member no. 70) PRIZES AND AWARDS 1998 Prize D. Dinis in Agronomy Sciences. Granted by the Prof. Agostinho da Silva Fundation / Montepio Geral Bank GRANTS AND FELLOWSHIPS JUNE 2012: Travel Bursary SEQAHEAD COST BM1006 ACTION NOV 2006 JUN 2009: Post-Doctoral Grant SFRH/BPD/17565/2004 FCT MAY 2008: JUL 2004 MAR 2005: Short Visit & Exchange Grant Researcher EUROPEAN SCIENCE FOUNDATION AGRO 8.1 (AGRO 518) JUL 2000 JUN 2004: PhD Student SFRH/BD/1133/2000 FCT JAN 1997 DEC 1999: Young Graduate Researcher SFRH/BIC/3365 FCT 6

7 LIST OF PUBLICATIONS: THESIS: Goulão LF (2005). Identification, cdna cloning and expression, and enzymatic activity of proteins involved in ripening and softening of apple (Malus x domestica Borkh. cv Mondial Gala ) fruits. PhD Thesis. Instituto Superior de Agronomia, Lisbon, 269 pp. Goulão LF (1996). Caracterização de germoplasma de Trifolium subterraneum L. Degree in Agronomic Engineering Thesis. Instituto Superior de Agronomia, Lisbon, 87 pp. BOOK CHAPTERS: Goulao LF and Oliveira CM (2014). Multilocus profiling with AFLP, ISSR and SAMPL In: Molecular Plant Taxonomy: Methods and Protocols (Ed.: P. Besse). Methods in Molecular Biology Series, Vol. 1115, Humana Press Inc, Springer Publishing, USA. pp (ISBN ) Goulao LF, Fernandes JC, Lopes P and Amâncio S (2012). "Tackling the cell wall of the grape berry". In: The Biochemistry of the Grape Berry (Ed.: H. Gerós, M.M. Chaves and S. Delrot). Bentham Science Publishers. pp (eisbn: ) Goulao LF, Almeida DP and Oliveira CM (2010). "Effect of enzymatic reactions on texture of fruits and vegetables". In: Enzymes in Fruit and Vegetable Processing: Chemistry and Engineering Applications (Ed.: A. Bayindirli). CRC Press, USA. pp (ISBN: ) PAPERS IN INTERNATIONAL SCIENTIFIC PERIODICALS WITH REFEREE: (ISI h-index = 11) Guenha R, Salvador BV, Rickman J, Goulao LF, Muocha IM and Carvalho MO (2014). Hermetic storage with plastic sealing to reduce insect infestation and secure paddy seed quality: a powerful strategy for rice farmers in Mozambique. Journal of Stored Products Research (DOI: /j.jspr ) Lara I, Belge B and Goulao LF (2014). The fruit cuticle as a modulator of postharvest quality. Postharvest Biology and Technology 87: Maquia I, Muocha I, Naico A, Martins N, Gouveia M, Andrade I, Goulao LF and Ribeiro AI (2013). Molecular, morphological and agronomic characterization of the sweet potato (Ipomoea batatas L.) germplasm collection from Mozambique: genotype selection for drought prone regions. South African Journal of Botany 88: Ramalho JC, Fortunato AS, Goulao LF and Lidon FC (2013). Cold-induced changes in mineral content in leaves of Coffea spp.: Identification of descriptors for tolerance assessment. Biologia Plantarum 57: Fernandes JC, García Angulo P, Goulao LF, Acebes JL and Amâncio S (2013). Mineral stress affects the cell wall composition of grapevine (Vitis vinifera L.) callus. Plant Science : Maquia I, Ribeiro N, Silva V, Bessa F, Goulao LF and Ribeiro AI (2013). Genetic diversity of Brachystegia boehmii Taub. and Burkea africana Hook. f. across a fire gradient in Niassa National Reserve, northern Mozambique. Biochemical Systematics and Ecology 48: Sousa PMF, Videira MAM, Bohn A, Hood BL, Conrads TP, Goulao LF and Melo AMP (2012). The aerobic respiratory chain of Escherichia coli: from genes to supercomplexes. Microbiology 158: Goulao LF, Fortunato AS and Ramalho JC (2012). Selection of reference genes for normalizing quantitative realtime PCR gene expression data with multiple variables in Coffea spp. Plant Molecular Biology Reporter 30: Goulao LF, Vieira-Silva S and Jackson PA (2011). Association of hemicellulose- and pectin-modifying gene expression with Eucalyptus globulus secondary growth. Plant Physiology and Biochemistry 49: Goulao LF, Cosgrove DJ and Oliveira CM (2008). Cloning, characterisation and expression analyses of cdna clones encoding cell wall-modifying enzymes isolated from ripe apples. Postharvest Biology and Technology 48: Goulao LF and Oliveira CM (2008). Cell wall modifications during fruit ripening: when a fruit is not the fruit. Trends in Food Science & Technology 19: Goulao LF, Santos J, Sousa I and Oliveira CM (2007). Patterns of enzymatic activity of cell wall-modifying enzymes during growth and ripening of apples. Postharvest Biology and Technology 43: Goulao LF and Oliveira CM (2007). Molecular identification of novel differentially expressed mrnas up-regulated during ripening of apples. Plant Science 172: Carvalho L, Goulão L, Oliveira C, Gonçalves J and Amâncio S (2004). RAPD assessment for identification of clonal identity and genetic stability of in vitro propagated chestnut hybrids. Plant Cell Tissue and Organ Culture 77: Goulão L and Oliveira C (2001). Molecular characterisation of cultivars of apple (Malus x domestica Borkh.) using microsatellite (SSR and ISSR) markers. Euphytica 122:

8 Goulão L, Cabrita L, Oliveira C and Leitão J (2001). Comparing RAPD and AFLP markers in discrimination and estimation of genetic similarities in apple (Malus x domestica Borkh.) cultivars. Euphytica 119: Monte-Corvo L, Goulão L and Oliveira C (2001). ISSR analysis of cultivars of pear and suitability of molecular markers for clone discrimination. Journal of the American Society for Horticultural Sciences 126: Goulão L, Valdiviesso T, Santana C and Oliveira C (2001). Comparison between phenetic characterisation using RAPD and ISSR markers, and phenotypic data of cultivated chestnut (Castanea sativa Mill.). Genetic Resources and Crop Evolution 48: Goulão L, Monte-Corvo L and Oliveira C (2001). Phenetic characterization of plum cultivars by high multiplex ratio markers: Amplified Fragment Length Polymorphisms and Inter-Simple Sequence Repeats. Journal of the American Society for Horticultural Sciences 126: Oliveira C, Mota M, Monte-Corvo L, Goulão L and Silva D (1999). Molecular typing of Pyrus based on RAPD markers. Scientia Horticulturae 79: INVITED REVIEWS AND OPPINION PAPERS: Goulao LF (2010). Pectin de-esterification and fruit softening: revisiting a classical hypothesis. Stewart Postharvest Review 6. pp. 1-12(12). PAPERS IN INTERNATIONAL CONFERENCE PROCEEDINGS: Domingos S, Scafidi P, Oliveira CM, di Lorenzo R and Goulao LF (2013). Effects of light reduction at bloom on fruit set in Black Magic table grape cv in early and late production cycles. Journal of Viticulture and Enology (Ciência e Técnica Vitivinícola) 28: (with Oral Communication) Ramalho JC, Rodrigues AP, Semedo JN, Costa C, Pais I, Fortunato AS, Palos I, Batista-Santos P, Goulao LF, Maia I, Scotti-Campos P, Máguas C, Nesi A, Damatta FM and Lidon FC (2013). Impact of long-term increased ambient CO2 on the photosynthetic functioning of Coffea arabica and C. canephora leaves. Proceedings of the 24th International Conference on Coffee Science, ASIC (Association for Science and Information on Coffee) 2012, pp (CD-ROM) (with Oral Communication) Goulao LF (2012). Regulation of postharvest fruit softening: taking pictures off the wall. In: Avances en Poscosecha de frutas y hortalizas (Ed.: I. Recasens, J. Graell and G. Echeverría). Lleida. pp (ISBN: ). (with Plenary Oral Communication) Batista-Santos P, Goulão LF, Partelli F, Campos PS, Pais IP, Fortunato AS, Ribeiro AI and Ramalho JC (2011). The effect of low temperature on metabolism of membrane lipids in Coffea sp. and associated gene expression. Proceedings of the ASIC (Association for Science and Information on Coffee) 2010: The 23 rd International Conference on Coffee Science, pp (CD-ROM) (with Poster) Goulão LF and Oliveira CM (2005). Patterns of temporal mrna expression and enzyme activity of pectolytic and non-pectolytic cell-wall degrading enzymes in growth and ripening of apple fruits. Acta Horticulturae 682: (with Oral Communication) Monte-Corvo L, Goulão L and Oliveira C (2002). Discrimination of pear cultivars with RAPD, AFLP, and ISSR. Acta Horticulturae 596: (with Poster) PAPERS IN NATIONAL SCIENTIFIC PERIODICALS WITH REFEREE: Goulão L, Monte-Corvo L, Mota M, Silva D and Oliveira C (1999). Caracterização de cultivares de macieira (Malus pumila Mill.) e de pereiras (Pyrus spp.) por amplificação aleatória de DNA (RAPD). Revista de Ciências Agrárias XXII(2): Farinha N, Brito A, Goulão L and Carneiro J (1999). Avaliação de linhas avançadas de Trifolium subterraneum L. em selecção nas condições de Elvas. Pastagens e Forragens 20: Goulão L, Farinha N, Tavares-de-Sousa M and Martins J (1999). Caracterização morfológica de linhas de Trifolium subterraneum L. com base em análise multivariada. Pastagens e Forragens 20: PAPERS IN NATIONAL CONFERENCE PROCEEDINGS: Ribeiro AI and Goulao LF (2013). Agrobiodiversidade: oportunidades e desafios para a resolução de problemas agro-ambientais e promoção do desenvolvimento socio-económico. Actas da Conferência do Sector Agrário e Desenvolvimento Rural em Moçambique: Transformação Estrutural e Competitividade do Sector Agrário (in press) Domingos S, Pesquita C, Couto F, Goulao LF and Oliveira C (2011). Improving Gene Functional Analysis: a Study on Ethylene-induced Leaf Abscission Using the Gene Ontology and ProteInOn. INForum 2011 Atas do Terceiro Simpósio em Informática (Ed.: R. Barbosa, L. Caires), pp (with Oral Communication) 8

9 Fortunato AS, Goulao LF, Leitão AE, Rodrigues AD, Jackson P, Partelli FL, Ribeiro AI and Ramalho JC (2011). The role of the antioxidantive system in Coffea sp. acclimation to simultaneous chilling and drought stress. VII Simpósio de Pesquisa dos Cafés do Brasil (CD-ROM), August, Araxá, MG, Brazil, 6 pp (with Poster) Alpalhão A, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I e Oliveira CM (2009). Efeito do 1- metilciclopropeno em pêra Rocha em diferentes estados de maturação armazenadas em atmosfera normal. Actas Portuguesas de Horticultura 11: (with Poster) Dionísio L, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I e Oliveira CM (2009). Efeito do 1- metilciclopropeno em maçãs Royal Gala armazenadas em atmosfera normal. Actas Portuguesas de Horticultura 11: (with Oral Communication) Neto C, Goulão L, Clemente J, Gomes MH, Duarte P, Almeida D e Oliveira C (2005). Efeito do 1-metilciclopropeno em maçãs e pêras armazenadas em atmosfera normal. Actas Portuguesas de Horticultura 8: (with Poster) Goulão L and Oliveira CM (2004). Identification, genetic expression and enzymatic activity of ripening and softening related proteins in apple fruits. Maturação e Pós-Colheita: Frutos e Hortícolas (Ed.: MG Barreiro), INIAP/EAN. pp (with Oral Communication) COMMUNICATIONS 1 : INVITED PLENARY COMMUNICATIONS: Ribeiro AI, Neves L, Goulao LF, Zacarias A, Melo AMP and Ribeiro N (2014). Enhancing scientific knowledge impact in Mozambique. AGRINATURA Science Days: Growing Together: Family Farming and Agricultural Sciences transforming World Food Systems, May 5-7, Vienna, Austria Goulao LF, Vaz Tomé C, Leitão AE, Martins AC and Melo AMP (2013). Saber Tropical, Sabor Tropical. 53º Encontro dos Descobrimentos: A Indústria do Doce no Mercado Internacional, November 14-15, Lisbon, Portugal Goulao LF and Melo AMP (2013). O IICT na Investigação e Cooperação para a Segurança Alimentar na CPLP. Conferência Segurança alimentar e desafios da saúde em África - Dia de África 2013; 50º Aniversário da União Africana, Grupo de Embaixadores Africanos, May 25, Lisbon, Portugal [Oral Presentation by L. Goulao] Goulao LF (2013). Biotechnology towards a productive agriculture in challenging climatic environments. II International Seminar on Biotechnology: Opportunities of Economic and Social Development in Valorization and Sustainable Management of Bioresources. May 22-24, Maputo, Mozambique Goulao LF (2013). Research progresses and challenges in biodiversity to food and nutritional security towards climate-smart agriculture in Mozambique. Policy Workshop on Climate Change Adaptation for Agriculture in Mozambique: Challenges for Agriculture Research, February 28, Maputo, Mozambique Goulao LF (2012). Regulation of postharvest fruit softening: taking pictures off the wall. POST LLEIDA VII Iberian Postharvest Symposium. October 1-4, Lleida, Spain. Ribeiro A and Goulao LF (2012). Biodiversidade e desenvolvimento sustentável. Cluster Tecnologias Tropicais : Biologia e Medicina Tropicais, AITECOeiras, May 29, Oeiras, Portugal ORAL COMMUNICATIONS: Domingos S, Nóbrega H, Cardoso V, Goulao LF and Oliveira CM (2014). Utilização de redes de ensombramento para monda de flores em uva de mesa Thompson Seedless e Crimson Seedless. 3º Simpósio Nacional de Fruticultura, to be held 4-5 December, Vila Real, Portugal [accepted; ; to be presented by C. Oliveira] Domingos S, Nóbrega H, Cardoso V, Ramalho JC, Oliveira CM and Goulao LF (2014). Effect of shade and gibberellic acid applied during bloom on fruit set in Thompson and Crimson Seedless table grape cultivars. 7th International Table Grape Symposium, to be held November, Mildura, Victoria Australia. [accepted; to be presented by S. Domingos] Belge B, Goulao LF, Comabella E, Graell J and Isabel Lara (2014). Postharvest heat and CO2 shocks induce changes in cuticle composition and cuticle-related gene expression in October Sun peach fruit. POST 14 VALENCIA - VIII Iberian Maturation and Postharvest Symposium. October 21-23, Valencia, Spain. [to be presented by I. Lara] Fernandes JC, Cobb F, Tracana S, Costa G, Valente I, Goulao LF and Amâncio S (2014). Relating water stress conditions to grape berry skin mechanical properties and cell wall composition and expression of remodeling genes. 2nd Annual Meeting of the COST FA 1106 Fruit Quality, to be held September, Chania, Greece. [accepted; to be presented by S. Amâncio] 1 Blue font: Communications under the scope of Research or Education for Development in tropical countries 9