Full Name: Luís Filipe Sanches Goulão

CURRICULUM VITAE LUIS F.S. GOULÃO PERSONAL DATA Full Name: Luís Filipe Sanches Goulão Nationality: Portuguese Birth Place: Carnaxide, Oeiras Date of Birth: July, 1973 Institutional Address: Tropical Research Institute [Instituto de Investigação Científica Tropical IICT, IP] BioTrop-Agri4Safe (Pólo Mendes Ferrão) Pavilhão de Agro-Indústrias e Agronomia Tropical (3º piso) Tapada da Ajuda, 1349-017 Lisboa PORTUGAL Phone: +351 213 617 247 E-mail: goulao@iict.pt EDUCATION 2006 - PhD IN AGRONOMIC ENGINEERING Postharvest Physiology and Molecular Biology of Fruits TECHNICAL UNIVERSITY OF LISBON (SCHOOL OF AGRICULTURAL SCIENCES; ISA/UTL), LISBON - PORTUGAL 1999 - MSci IN SUSTAINABLE AGRICULTURE AND HORTICULTURE (Scholar Part) TECHNICAL UNIVERSITY OF LISBON (SCHOOL OF AGRICULTURAL SCIENCES; ISA/UTL), LISBON PORTUGAL 1996 - DEGREE IN AGRONOMIC ENGINEERING Plant Breeding TECHNICAL UNIVERSITY OF LISBON (SCHOOL OF AGRICULTURAL SCIENCES; ISA/UTL), LISBON PORTUGAL RESEARCH INTERESTS - Agricultural RESEARCH FOR DEVELOPMENT (R4D) - FOOD AND NUTRITIONAL SECURITY AND SAFETY (hunger and "hidden hunger") - COLLABORATIVE RESEARCH with CPLP countries PLANT SCIENCES APPLIED TO ECONOMICALLY IMPORTANT CULTIVATED SPECIES - Postharvest biology and technology: fruit ripening, quality and storage - Domestication and exploitation of native tropical fruits towards food security, nutrition and health - Molecular, biochemical and enzymatic modifications of the plant cell wall with agronomic/industrial relevance - Applications of molecular markers in plant management, breeding and agriculture - Identification, expression and functional characterization of genes with agronomic and industrial relevance in economically important plant species TRAINING IN INTERNATIONAL LABORATORIES 2008 - Visiting scholar at the Department of Genetics - University of Georgia, Athens, GA - USA (supervision of Prof. Chung-Jui Tsai and Dr. Scott Harding), September-November 2004 Visiting scholar at the Department of Biology - Pennsylvania State University, PA USA (supervision of Prof. Daniel Cosgrove), March-June 1

ADVANCED TRAINING 2012 European Cooperation in Science and Technology (COST) Course: "Next Generation Sequencing Data Analysis with Chipster, 13-15 June, Helsinki, Finland 2010 The Gulbenkian Training Programme in Bioinformatics Course: Automatic Annotation and Data Mining, 13-14 May, Oeiras, Portugal 2009 The Gulbenkian Training Programme in Bioinformatics Course: Workflows and Programatically Accessible Tools, 16-17 November, Oeiras, Portugal 2009 The Gulbenkian Training Programme in Bioinformatics Course: Microarray Data Analysis using R and Bioconductor, 14-18 September, Oeiras, Portugal 2008 European Science Foundation for Functional Genomics (ESFFG) Workshop: "Advanced large scale gene expression profiling focus on mirnas, ChIP-on-chip and whole genome sequencing", 19-23 May, Turku, Finland 2002 Workshop "Coconut genetic resources management using microsatellite kit and dedicated statistical software", 15-24 April, Montpellier, France SCIENTIFIC APPOINTMENTS SINCE JUL 2009: Research Associate ( Investigador Auxiliar ) at Tropical Research Institute (IICT), Oeiras. Head of the Agri4Safe Group since Nov 2012 NOV 2006 - JUN 2009: Post-Doctoral Fellow at Plant Cell Wall Group Instituto de Tecnologia Química e Biológica (ITQB/UNL), Oeiras JAN 2005 - OCT 2006: Graduate Student at Plant Cell Wall Group - Instituto de Tecnologia Química e Biológica (ITQB), Oeiras JUL 2004 - MAR 2005: Research Grant for Program AGRO 8.1 at Technical University of Lisbon (School of Agricultural Sciences; ISA/UTL), Lisbon / Painho Fruit Storage Central, Cadaval JUL 2000 - JUL 2004: PhD Student at Horticulture Department - Technical University of Lisbon (School of Agricultural Sciences; ISA), Lisbon JAN 1997 - DEC 1999: Young Graduate Researcher Fellow at Technical University of Lisbon (School of Agricultural Sciences; ISA), Lisbon / University of Algarve (Faculty of Engineering and Natural Resources), Faro / Vieira Natividade National Lab for Fruit Research (ENFVN), Alcobaça MAR 1996 - DEC 1996: Trainee ( Estagiário ) at Department of Forage Crops - National Lab for Plant Breeding (ENMP), Elvas PARTICIPATION IN RESEARCH PROJECTS STARTING MAY 2013: PTDC/AGR-PRO/3386/2012: Implications of environmental changes of CO2 and temperature at the plant and bean quality levels in the coffee tree (Coffea spp.) - multidisciplinary characterization of the impact and acclimation ability of the producing species. SINCE FEB 2013: FrutosNativos4SAN Mozambique [Project Coordinator] SINCE MAR 2012: PTDC/AGR-GPL/116923/2010: VitiShade - Abscission of seedless grapes: shedding the genes from the shade. [Project Coordinator] SINCE FEB 2012: PTDC/AGR-ALI/118477/2010: SafeFruit - Aureobasidium pullulans, a biofungicide for the control of postharvest decay in pears: insights into its action mechanisms. SINCE JAN 2012: PRODER 4.1 - PA nº 23923, partnership #373: PROdUVA [Project Coordinator at IICT] SINCE JAN 2011: PUBLIC-PRIVATE PARTNERSHIP IICT/GEOCAPITAL HOLDINGS (Macau): Sustainable biodiesel production from Jatropha curcas in countries of the CPLP (Portuguese-speaking countries). DEC 2009 - DEC 2011: BIOVERSITY INTERNATIONAL F06: Bridging the divide between natural resource conservation strategies and the role of key forest genetic resources in Niassa National Reserve, Mozambique. NOV 2007 DEC 2010: PTDC/AGR-AAM/64078/2006: Molecular evaluation and characterization of tolerance mechanisms to adverse environmental conditions in Coffea sp. - central role of the oxidative stress control at leaf level. JAN 2005 JUN 2009: POCTI/AGR/46671/02: Cell wall proteins with roles in xylogenic programmes in eucalyptus. JAN 2004 - MAR 2005: AGRO 518: Improving postharvest quality and safety of pear, apple and kiwi. JUL 2000 - JUN 2004: POCTI/33733/99: Genetic expression and activity of non-pectolytic enzymes and involvement in apple physical properties. 2002: CIRAD/COGENT (France): Improving coconut production in Mozambique through germplasm characterization and evaluation of promising hybrids. (Project canceled in 2003 due to cancelling of funding) JAN 1997 - DEC 1999: PRAXIS/3./3.2/HORT/2163/95: Bio-molecular characterization of Portuguese cultivars of apple and pear. JAN 1997 - DEC 1999: PRAXIS/3/3.2/HORT/2143/95: Sanitation and breeding of Portuguese fruit tree cultivars using a biotechnological approach. 2

JAN 1997 - DEC 1999: PRAXIS/3/3.2/HORT/2136/95: Study of the biology and sexual reproduction in the chestnut and almond trees: Genetic and fruit breeding. 1996: PAMAF 3088: Study of annual pastures under different soil conditions: annual alfalfas and sub-clovers. SUPERVISING EXPERIENCE (at graduate level) PhD THESIS: SINCE JAN 2011 Sara Nobre Gonçalves Domingos. Thesis: Thinning via shading in seedless table grapes: Unravelling the physiological and molecular basis of berries abscission (Co-orientation with Prof. Cristina Moniz Oliveira (ISA/UTL); grant: SFRH/BD/69076/2010) SINCE SEP 2009 João Carlos Martins Fernandes. Thesis: Mineral stress response of Vitis cell wall transcriptome: Identification, characterization and expression of genes from key families involved in wall biosynthesis and modification (Co-orientation with Prof. Sara Amâncio (ISA/UTL); grant: SFRH/BD/64047/2009) MSci THESIS: JAN 2012 MAR 2013 Ivete Sandra Maquia. Thesis: Molecular characterization of forest genetic resources from the Miombo woodlands at the Niassa National Reserve: development of barcode- and ISSR- markers, Eduardo Mondlane University, Mozambique (Co-orientation with Dr. Ana Ribeiro (IICT)) JAN 2012 MAR 2013 Elias Manuel Amadeu Militão. Thesis: Genetic characterization of Afzelia quanzensis Welw. populations from the Michafutene forest, Eduardo Mondlane University, Mozambique (Coorientation with Dr. Ana Ribeiro (IICT)) CAPACITY BUILDING: SINCE APR 2013 Vânia Alexandra da Silva Cardoso (Researcher; grant associated with the VitiShade project): Searching for molecular signaling of flower/fruitlet abscission in Vitis vinifera AUG 2012 DEC 2012 Ana Cláudia Raposo (Researcher; grant associated with the VitiShade project): Agronomic, physiological and molecular assessment of seedless grapes induced for flower/fruitlet abscission under field conditions FEB 2011 JUL 2012 Vanessa Alexandra Victoriano dos Santos (Degree in Biotechnology; Universidade Lusófona de Humanidades e Tecnologias (ULHT)): Molecular characterization of the IICT Jatropha curcas germplasm collection JAN 2011 AUG 2011 Juliana Barbosa (PhD student; Lavras Federal University (UFLA), Brazil): Association of coffee quality with physical and chemical parameters of the green bean DEC 2010 Celestino Uamusse (Researcher, Center for Research and Development in Ethonobotany, Mozambique): "Basic molecular biology and bioinformatics methods" (co-orientation with Dr. Ana Ribeiro and Dr. Ana Fortunato (IICT)) TEACHING In Portugal: SINCE 2007: Two lab classes / year (Biophysics of the primary cell wall) in Plant Biology classes of the Degree in Biology; 3 rd semester (ISA/UTL, Lisbon) 2012: Two lectures in Genetic Engineering classes of the Degree in Plant Biotechnology; 3 rd semester (Coimbra Agronomy School, Coimbra) 2011: Lecture The paradoxical characteristics of the primary cell wall in the Workshop Hidden Stories Within Cell Walls (ISA/UTL, Lisbon) 2010 2011: One lab class in Methods of Molecular Analysis classes of the MSci in Functional Biology (ISA/UTL) 2010: One lecture in Modified Organisms classes of the MSci in Biofuels; 1 st semester (Coimbra Agronomy School, Coimbra) 2004: One lecture in Plant Development classes of the Degree in Agronomic Engineering; 9 th semester (ISA/UTL) 2001 2004: Assistant in Plant Biotechnology lab classes of the Degree in Agronomic Engineering; 6 th semester (ISA/UTL) 3

In Portuguese Speaking Countries (CPLP): SINCE 2011: Transcriptomics and Metabolomics (Module CB10 Functional Genomics) in Systems Biology classes of the Master (MSci) in Biotechnology (Eduardo Mondlane University (UEM), Maputo, Mozambique) 2013: Theoretical and practical workshop Quantification of gene expression using quantitative real-time PCR (Centre of Biotechnology (CB) - Eduardo Mondlane University (UEM), Maputo, Mozambique), 20-21 February. JURY PARTICIPATION IN ACADEMIC DEGREES (at graduate level) PhD DISSERTATIONS: 2010 - José Mário Leitão Ribeiro. Dissertation: The importance of extensin network formation in the definition of primary cell wall properties - PhD in Biological Sciences ITQB/UNL, Oeiras, 29 th January (3 rd opponent). MSci DISSERTATIONS: 2012 Frederic Cobb. Dissertation: Effect of irrigation on berry and skin cell wall composition in the grape varieties Touriga Nacional and Trincadeira - MSci Vinifera EuroMaster, European Master of Sciences of Viticulture and Oenology ISA/UTL, Lisbon, 4 th December (Opponent). 2012 - Carlos André Silva Correia. Dissertation: Flower and berry thinning by shading in Sugraone and Crimson Seedless table grapes - MSci in Agronomy ISA/UTL, Lisbon, 16 th March (Main opponent). EDITORIAL ACTIVITY IN SCIENTIFIC JOURNALS MEMBER OF THE EDITORIAL BOARD: SINCE 2012: ISRN Agronomy, International Scholarly Research Network (Open Access) SINCE 2011: International Journal of Plant Physiology and Biochemistry (IJPPB), Academic Journals (Open Access) EDITOR: 2013: The Scientific World Journal Agronomy (Hindawi Publishing Corporation): Lead Editor of the Special Issue on Exploitation of Tropical Native Fruit Crops towards Nutrition, Food Security, and Safety PEER REVIEWER: 83 manuscripts (MS) to date: Scientia Horticulturae - 45 MS; Journal of Agricultural and Food Chemistry 5 MS; Food Control 3 MS; HortScience 2 MS; Plant Cell Tissue and Organ Culture 2 MS; Carbohydrate Polymers 2 MS; Tree Physiology 2 MS; Functional Plant Biology 1 MS; Phytochemistry 1 MS; Industrial & Engineering Chemical Research 1 MS; Journal of Food Engineering 1 MS; Journal of the American Society of Horticultural Sciences - 1 MS; Journal of Horticultural Science & Biotechnology 1 MS; Plant Systematics and Evolution 1 MS; The Scientific World Journal: Agronomy 1MS; ISRN Agronomy 8 MS; African Journal of Biotechnology 1 MS; International Journal of Plant Physiology and Biochemistry 1 MS; International Journal of Food, Agriculture and Environment 1 MS; Emirates Journal of Food and Agriculture 1 MS; African Journal of Agricultural Research 1 MS; Journal of Tropical Forest Science 1 MS ORGANIZATION OF SCIENTIFIC MEETINGS 2012 Member of the Organizing Committee of the: MINERAL IMPROVED CROP PRODUCTION FOR HEALTHY FOOD AND FEED: ENHANCED NUTRITIONAL VALUE OF PLANT-DERIVED FOOD OR FEED (3 rd Annual Workshop - COST Action FA0905. All WGS+MC Meeting), 23-25 October, Lisbon, Portugal. 2012 Member of the Scientific Committee of the "MOZAMBIQUE INTERNATIONAL CONGRESS: HISTORY, MEMORY AND SCIENCE, 24-26 October, Lisbon, Portugal. 2006 - Member of the Local Organizing Committee of the "PEROXIDASE 2006 CONGRESS", 6-9 July, Aveiro, Portugal. 4

EVALUATOR IN INTERNATIONAL PROJECT CALLS 2011 AND 2012 - French National Research Agency (Agence Nationale de la Recherche; ANR). Committee: "Interface Biology-Physics, Interface Biology-Chemistry and Biotechnology". PRIZES AND AWARDS 1998 Prize D. Dinis in Agronomy Sciences. Granted by the Prof. Agostinho da Silva Fundation / Montepio Geral Bank GRANTS AND FELLOWSHIPS JUNE 2012: Travel Bursary SEQAHEAD COST BM1006 ACTION NOV 2006 JUN 2009: Post-Doctoral Grant SFRH/BPD/17565/2004 FCT MAY 2008: JUL 2004 MAR 2005: Short Visit & Exchange Grant Researcher EUROPEAN SCIENCE FOUNDATION AGRO 8.1 (AGRO 518) JUL 2000 JUN 2004: PhD Student SFRH/BD/1133/2000 FCT JAN 1997 DEC 1999: Young Graduate Researcher SFRH/BIC/3365 FCT LIST OF PUBLICATIONS: THESIS: Goulão LF (2005). Identification, cdna cloning and expression, and enzymatic activity of proteins involved in ripening and softening of apple (Malus x domestica Borkh. cv Mondial Gala ) fruits. PhD Thesis. Instituto Superior de Agronomia, Lisbon, 269 pp. Goulão LF (1996). Caracterização de germoplasma de Trifolium subterraneum L. Degree in Agronomic Engineering Thesis. Instituto Superior de Agronomia, Lisbon, 87 pp. BOOK CHAPTERS: Goulao LF and Oliveira CM (2013). Length Polymorphism - Multilocus profiling: AFLP/ISSR/SAMPL In: Molecular Plant Taxonomy (Ed.: P. Besse). Methods in Molecular Biology Series, Humana Press Inc, Springer Publishing, USA (in press). Goulao LF, Fernandes JC, Lopes P and Amâncio S (2012). "Tackling the cell wall of the grape berry". In: The Biochemistry of the Grape Berry (Ed.: H. Gerós, M.M. Chaves and S. Delrot). Bentham Science Publishers. pp. 172-193 (eisbn: 978-1-60805-360-5) Goulao LF, Almeida DP and Oliveira CM (2010). "Effect of enzymatic reactions on texture of fruits and vegetables". In: Enzymes in Fruit and Vegetable Processing: Chemistry and Engineering Applications (Ed.: A. Bayindirli). CRC Press, USA. pp. 71-122 (ISBN: 9781420094336) INVITED REVIEWS AND OPPINION PAPERS: Goulao LF (2010). Pectin de-esterification and fruit softening: revisiting a classical hypothesis. Stewart Postharvest Review 6. pp. 1-12(12). PAPERS IN INTERNATIONAL SCIENTIFIC PERIODICALS WITH REFEREE (ISI h-index = 11): Fernandes JC, García Angulo P, Goulao LF, Acebes JL and Amâncio S (2013). Mineral stress affects the cell wall composition of grapevine (Vitis vinifera L.) callus. Plant Science 205 206: 111-120. Maquia I, Ribeiro N, Silva V, Bessa F, Goulao LF and Ribeiro AI (2013). Genetic diversity of Brachystegia boehmii Taub. and Burkea africana Hook. f. across a fire gradient in Niassa National Reserve, northern Mozambique. Biochemical Systematics and Ecology 48: 238 247. Sousa PMF, Videira MAM, Bohn A, Hood BL, Conrads TP, Goulao LF and Melo AMP (2012). The aerobic respiratory chain of Escherichia coli: from genes to supercomplexes. Microbiology 158: 2408 2418. 5

Goulao LF*, Fortunato AS and Ramalho JC (2012). Selection of reference genes for normalizing quantitative realtime PCR gene expression data with multiple variables in Coffea spp. Plant Molecular Biology Reporter 30: 741 759. (* corresponding author) Goulao LF, Vieira-Silva S and Jackson PA (2011). Association of hemicellulose- and pectin-modifying gene expression with Eucalyptus globulus secondary growth. Plant Physiology and Biochemistry 49: 873-381. Goulao LF*, Cosgrove DJ and Oliveira CM (2008). Cloning, characterisation and expression analyses of cdna clones encoding cell wall-modifying enzymes isolated from ripe apples. Postharvest Biology and Technology 48: 37-51. (* corresponding author) Goulao LF* and Oliveira CM (2008). Cell wall modifications during fruit ripening: when a fruit is not the fruit. Trends in Food Science & Technology 19: 4-25 - review article. (* corresponding author) Goulao LF, Santos J, Sousa I and Oliveira CM (2007). Patterns of enzymatic activity of cell wall-modifying enzymes during growth and ripening of apples. Postharvest Biology and Technology 43: 307-318. Goulao LF and Oliveira CM (2007). Molecular identification of novel differentially expressed mrnas up-regulated during ripening of apples. Plant Science 172: 306-318. Carvalho L, Goulão L, Oliveira C, Gonçalves J and Amâncio S (2004). RAPD assessment for identification of clonal identity and genetic stability of in vitro propagated chestnut hybrids. Plant Cell Tissue and Organ Culture 77: 23-27. Goulão L and Oliveira C (2001). Molecular characterisation of cultivars of apple (Malus x domestica Borkh.) using microsatellite (SSR and ISSR) markers. Euphytica 122: 81-89. Goulão L, Cabrita L, Oliveira C and Leitão J (2001). Comparing RAPD and AFLP markers in discrimination and estimation of genetic similarities in apple (Malus x domestica Borkh.) cultivars. Euphytica 119: 259-270. Monte-Corvo L, Goulão L and Oliveira C (2001). ISSR analysis of cultivars of pear and suitability of molecular markers for clone discrimination. Journal of the American Society for Horticultural Sciences 126: 517-522. Goulão L, Valdiviesso T, Santana C and Oliveira C (2001). Comparison between phenetic characterisation using RAPD and ISSR markers, and phenotypic data of cultivated chestnut (Castanea sativa Mill.). Genetic Resources and Crop Evolution 48: 329-338. Goulão L, Monte-Corvo L and Oliveira C (2001). Phenetic characterization of plum cultivars by high multiplex ratio markers: Amplified Fragment Length Polymorphisms and Inter-Simple Sequence Repeats. Journal of the American Society for Horticultural Sciences 126: 72-77. Oliveira C, Mota M, Monte-Corvo L, Goulão L and Silva D (1999). Molecular typing of Pyrus based on RAPD markers. Scientia Horticulturae 79: 163-174. PAPERS IN INTERNATIONAL CONFERENCE PROCEEDINGS: Domingos S, Scafidi P, Oliveira CM, di Lorenzo R and Goulao LF (2013). Effects of light reduction at bloom on fruit set in Black Magic table grape cv in early and late production cycles. Journal of Viticulture and Enology (Ciência e Técnica Vitivinícola) (in press) (with Oral Communication) Goulao LF (2012). Regulation of postharvest fruit softening: taking pictures off the wall. In: Avances en Poscosecha de frutas y hortalizas (Ed.: I. Recasens, J. Graell and G. Echeverría). Lleida. pp. 101-114 (ISBN: 978-84- 695-4683-3). (with Plenary Oral Communication) Batista-Santos P, Goulão LF, Partelli F, Campos PS, Pais IP, Fortunato AS, Ribeiro AI and Ramalho JC (2011). The effect of low temperature on metabolism of membrane lipids in Coffea sp. and associated gene expression. Proceedings of the ASIC (Association for Science and Information on Coffee) 2010: The 23 rd International Conference on Coffee Science, pp. 428-436. (CD-ROM) (with Poster) Goulão LF and Oliveira CM (2005). Patterns of temporal mrna expression and enzyme activity of pectolytic and non-pectolytic cell-wall degrading enzymes in growth and ripening of apple fruits. Acta Horticulturae 682: 91-98. (with Oral Communication) Monte-Corvo L, Goulão L and Oliveira C (2002). Discrimination of pear cultivars with RAPD, AFLP, and ISSR. Acta Horticulturae 596: 187-191. (with Poster) PAPERS IN NATIONAL SCIENTIFIC PERIODICALS WITH REFEREE: Goulão L, Monte-Corvo L, Mota M, Silva D and Oliveira C (1999). Caracterização de cultivares de macieira (Malus pumila Mill.) e de pereiras (Pyrus spp.) por amplificação aleatória de DNA (RAPD). Revista de Ciências Agrárias XXII(2): 65-82. Farinha N, Brito A, Goulão L and Carneiro J (1999). Avaliação de linhas avançadas de Trifolium subterraneum L. em selecção nas condições de Elvas. Pastagens e Forragens 20: 145-152. Goulão L, Farinha N, Tavares-de-Sousa M and Martins J (1999). Caracterização morfológica de linhas de Trifolium subterraneum L. com base em análise multivariada. Pastagens e Forragens 20: 63-74. 6

PAPERS IN NATIONAL CONFERENCE PROCEEDINGS: Domingos S, Pesquita C, Couto F, Goulao LF and Oliveira C (2011). Improving Gene Functional Analysis: a Study on Ethylene-induced Leaf Abscission Using the Gene Ontology and ProteInOn. INForum 2011 Atas do Terceiro Simpósio em Informática (Ed.: R. Barbosa, L. Caires), pp. 72-77. (with Oral Communication) Fortunato AS, Goulao LF, Leitão AE, Rodrigues AD, Jackson P, Partelli FL, Ribeiro AI and Ramalho JC (2011). The role of the antioxidantive system in Coffea sp. acclimation to simultaneous chilling and drought stress. VII Simpósio de Pesquisa dos Cafés do Brasil (CD-ROM), 22-25 August, Araxá, MG, Brazil, 6 pp (with Poster) Alpalhão A, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I e Oliveira CM (2009). Efeito do 1- metilciclopropeno em pêra Rocha em diferentes estados de maturação armazenadas em atmosfera normal. Actas Portuguesas de Horticultura 11: 224-230. (with Poster) Dionísio L, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I e Oliveira CM (2009). Efeito do 1- metilciclopropeno em maçãs Royal Gala armazenadas em atmosfera normal. Actas Portuguesas de Horticultura 11: 162-171. (with Oral Communication) Neto C, Goulão L, Clemente J, Gomes MH, Duarte P, Almeida D e Oliveira C (2005). Efeito do 1-metilciclopropeno em maçãs e pêras armazenadas em atmosfera normal. Actas Portuguesas de Horticultura 8: 382-389. (with Poster) Goulão L and Oliveira CM (2004). Identification, genetic expression and enzymatic activity of ripening and softening related proteins in apple fruits. Maturação e Pós-Colheita: Frutos e Hortícolas (Ed.: MG Barreiro), INIAP/EAN. pp. 145-149. (with Oral Communication) COMMUNICATIONS: INVITED PLENARY COMMUNICATIONS: Goulao LF (2012). Regulation of postharvest fruit softening: taking pictures off the wall. POST LLEIDA 2012 - VII Iberian Postharvest Symposium. October 1-4, Lleida, Spain. ORAL COMMUNICATIONS: Domingos S, Scafidi P, Oliveira CM, di Lorenzo R and Goulao LF (2013). Effects of light reduction at bloom on fruit set in Black Magic table grape cv in early and late production cycles. The 18th International Symposium GiESCO (Group of international Experts of vitivinicultural Systems for CoOperation) 2013, to be held 7-11 July, Porto, Portugal. [Presented by S. Domingos] (accepted) Ramalho JC, Rodrigues AP, Semedo J, Pais IP, Batista-Santos P, Goulao LF, Fortunato AS, Palos IM, Maia I, Scotti- Campos P, Damatta FM and Lidon FC (2012). Impact of long-term increased ambient CO2 on Coffea arabica and C. canephora photosynthetic functioning. ASIC (Association for Science and Information on Coffee) 2012: The 24th International Conference on Coffee Science Programme and Abstracts, 11 16 November, San José, Costa Rica. pp. 232 [Presented by J. Ramalho] Fernandes JC, García-Angulo P, Goulao LF, Acebes JL and Sara Amâncio (2012). Mineral stress alters cell wall cellulose and pectin methylesterification in grapevine (Vitis vinifera L.) callus. NutriPlanta 2012 - XIV Simposio Hispano-Luso de Nutrición Mineral de las Plantas, 23-26 July, Madrid, Spain. pp. 81 [Presented by J. Fernandes] Domingos S, Pesquita C, Couto F, Goulao LF and Oliveira C (2011). Improving Gene Functional Analysis: a Study on Ethylene-induced Leaf Abscission Using the Gene Ontology and ProteInOn. INForum 2011 - Simpósio em Informática (Bioinformatics Track - N66), September 8-9, Coimbra, Portugal. pp. 72-77 [Presented by S. Domingos] Fernandes JC, García-Angulo P, Goulao LF, Acebes JL and Amâncio S. (2011). Is there a correlation between primary cell wall (CW) composition and the expression of key CW biosynthesis and modification genes in grapevine callus under mineral stress? XII Congreso Hispano-Luso de Fisiologia Vegetal, June 21-24, Castelló de la Plana, Spain. pp. 33 [Presented by J. Fernandes] Dionísio L, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I, Oliveira CM (2006). Efeito do 1- metilciclopropeno em maçãs Royal Gala armazenadas em atmosfera normal em dois anos consecutivos de ensaio. I Simpósio Nacional de Fruticultura: Programa e Resumos das Comunicações, 12-13 October, Alcobaça, Portugal. pp.49 [Presented by C. Neto] Goulão L and Oliveira CM (2004). Identification, genetic expression and enzymatic activity of ripening and softening related proteins in apple fruits. IV Iberian Symposium on Maturation and Postharvest 2004, 6-9 October, Oeiras, Portugal. pp. 145-149 [Presented by L. Goulão] Goulão L and Oliveira CM (2004). Patterns of temporal mrna expression and enzyme activity of pectolytic and non-pectolytic cell-wall degrading enzymes in growth and ripening of apple fruits. 5th International Postharvest Symposium, 6-11 June, Verona, Italy. pp. 6 [Presented by L. Goulão] 7

Goulão L, Monte-Corvo L and Oliveira C (1999). Identification of European and Japanese plum cultivars based on ISSR (Inter-Simple Sequence Repeats) markers. Resumos do 2º Workshop de Biotecnologia de Plantas, 6-7 May, Faro, Portugal. pp.21 [Presented by L. Goulão] Farinha N, Carneiro J, Brito A and Goulão L (1998). Avaliação de linhas avançadas de Trifolium subterraneum L. em selecção nas condições de Elvas. Programa e Resumos da XIX Reunião de Primavera da Sociedade Portuguesa de Pastagens e Forragens, May, Castelo Branco, Portugal. pp.12. [Presented by N. Farinha] POSTERS IN CONFERENCES: Domingos S, Cardoso VA, Oliveira CM and Goulao LF (2013). Molecular signatures of floweret abscission signaling in Vitis vinifera. FV2013: XIII Congresso Luso-Espanhol de Fisiologia Vegetal, 24-28 July, Lisbon, Portugal (submitted) Borém FM, Barbosa JN, Tosta MF, Leitão AE, Ribeiro DE, Cirillo MA, Ramalho JC, Sousa IN and Goulao LF (2012). Hardness of the coffee green bean relates to the environmental-driven sensorial quality of the beverage. ASIC (Association for Science and Information on Coffee) 2012: The 24th International Conference on Coffee Science Programme and Abstracts, 11 16 November, San José, Costa Rica. pp. 115 Maçãs B, Coutinho JP, Almeida AS, Costa AR, Leitão AE, Leitão AL, Duarte MP, da Silva MMA, Fernando AL, Scotti- Campos P, Semedo J, Pais IP, Ramalho JC, Ribeiro AI, Goulão LF, Simões F, Matos J, Reboredo FH, Almeida M, Galinha C, Ribeiro V and Lidon FC (2012). Wheat crop in Portugal biofortification prospective. COST ACTION FA 0905 Third Annual Workshop: Enhanced Nutritional Value of Plant-Derived Food or Feed program & Abstracts, 23-26 October, Almada, Portugal. pp. 50 (+ 4 supportive posters) Sánchez C, Nunes A, Goulao LF, Coimbra MA and Barreiro G (2012). Insights into the mechanisms underlying the antagonistic action of A. pullulans against postharvest decay in pears. Resúmens of the POST LLEIDA 2012 - VII Iberian Postharvest Symposium. October 1-4, Lleida, Spain. pp. 105 Sousa PMF, Videira MAM, Bohn A, Goulão LF, Hood BL, Conrads TP and Melo AMP (2012). An integrated perspective of the Escherichia coli aerobic respiratory chain. European BioEnergetic Congress (EBEC). September 15-21, Freiburg, Germany. In: Biochimica et Biophysica Acta (BBA) Bioenergetics 1817, Supplement. pp. S141 Fernando AL, Simões F, Costa AR, Matos J, Reboredo FH, Leitão AE, Ramalho RJ, Almeida AS, Maçãs B, Coutinho J, Scotti-Campos P, Pais IP, Silva MMA, Duarte MP, Goulão LF, Ribeiro AI and Lidon FC (2011). I. Simultaneous biofortification of Zn and Fe in Triticum aestivum L. A transgeneration survey. Abstracts of the First Annual Conference Mineral Improved Crop Production for Healthy Food and Feed COST FA0905, 23-26 November, Venice, Italy. pp. 61 (+ 1 communication and 4 supporting posters) Goulao LF, Fortunato AS, Ribeiro AI, Partelli FL and Ramalho JC (2011). A multiple analytical approach for normalizing gene expression data of Coffea acclimation to changing environments. Livro de resumos da XIV Reunião Latino-Americana de Fisiologia Vegetal / XIII Congresso Brasileiro de Fisiologia Vegetal, 19-22 September, Búzios, RG, Brazil. pp.215 Fortunato AS, Goulao LF, Leitão AE, Rodrigues AD, Jackson P, Partelli FL, Ribeiro AI and Ramalho JC (2011). The role of the antioxidantive system in Coffea sp. acclimation to simultaneous chilling and drought stress. VII Simpósio de Pesquisa dos Cafés do Brasil, 22-25 August, Araxá, MG, Brazil, 6 pp Goulao LF, Fortunato AS, Jackson PA, Rodrigues A, Ribeiro AI and Ramalho JC (2010). Differential responses of the antioxidant defense system to chilling and drought stresses in Coffea sp. genotypes - gene expression and antioxidant reinforcement. Congresso Nacional de Bioquímica, 15-17 December, Porto, Portugal. pp.305 Batista-Santos P, Partelli F, Campos PS, Pais IP, Fortunato AS, Goulão LF, Ribeiro AI and Ramalho JC (2010). The effect of low temperature on metabolism of membrane lipids in Coffea sp. and associated gene expression. ASIC (Association for Science and Information on Coffee) 2010: The 23rd International Conference on Coffee Science - Programme & Abstracts, 3 8 October, Bali, Indonesia. pp.145 Fernandes JC, Lopes PC, Goulao LF and Amâncio S (2010). Exploring the Vitis cell wall transcriptome. XII Cell Wall Meeting Abstracts Book, 25-30 July, Porto, Portugal. pp.65 Goulao LF, Vatulescu AD, Ribeiro A, Ramalho JC, Jackson PA (2008). Developing a high trough-put method to identify class III and ascorbate peroxidases of coffee differentially expressed under conditions of stress. 8th International Peroxidase Symposium, 20-24 August 2008, Tampere, Finland. Goulao LF, Vieira-da-Silva S, Jackson PA (2007). Identification of hemicellulose- and pectin-modifying genes expressed during Eucalyptus wood formation. XI Cell Wall Meeting, 12-17 August, Copenhagen, Denmark. Physiologia Plantarum 130(4) Abstract 230 Goulao LF, Vieira-da-Silva S, Jackson PA (2007). Cell wall modifications during xylogenesis in Eucalyptus globulus involves divergent transcription of several members from candidate families. IUFRO Tree Biotechnology 2007, 3-8 June, Ponta Delgada - Azores, Portugal. pp.svi.33 Alpalhão A, Neto C, Goulão L, Clemente J, Henriques J, Lourenço I, Oliveira CM (2006). Efeito do 1- metilciclopropeno em pêra Rocha com diferentes estados de maturação armazenadas em atmosfera normal. I Simpósio Nacional de Fruticultura: Programa e Resumos das Comunicações, 12-13 October 2006, Alcobaça, Portugal. pp.61 Goulao LF, Vatulescu A, Jackson PA (2006). Two peroxidases from Eucalyptus wood with role in cellular growth. Peroxidase 2006: Abstracts & Programme, 6-9 July 2006, Aveiro, Portugal. pp.46 8

Neto C, Goulão L, Clemente J, Gomes MH, Duarte P, Almeida D and Oliveira C (2005). Efeito do 1- metilciclopropeno em maçãs e pêras armazenadas em atmosfera normal. V Congresso Ibérico de Ciências Hortícolas/IV Congresso Iberoamericano de Ciências Hortícolas, May 2005, Porto, Portugal. pp.382-389 Goulão L and Oliveira CM (2002). Cloning and characterization of a cdna clone encoding an expansin isoform with expression in ripening apples. Programa e Resumos do XIII Congresso Nacional de Bioquímica, 5-7 December 2002, Lisboa, Portugal. pp.57 Goulão L and Oliveira, CM (2002). Evidence of the presence of several isoforms of endo-beta-1,4-glucanases, xyloglucan endotransglycosylase and expansins in over-ripe apples. 13th Congress of the Federation of European Societies of Plant Physiology: Book of Abstracts, 2-6 September 2002, Hersonissos - Crete, Greece. pp.338 Monte-Corvo L, Goulão L and Oliveira C (2000). Comparison of molecular markers (RAPD, AFLP, ISSR and SSR) on pear (Pyrus communis L.). VIII International Symposium on Pear Growing (ISHS), 4-9 September 2000, Ferrara-Bologna, Italy. pp.47-48 Goulão L, Farinha N, Sousa M and Martins J (1999). Caracterização morfológica de linhas de Trifolium subterraneum L. com base em análise multivariada. Programa e Resumos da XX Reunião de Primavera da Sociedade Portuguesa de Pastagens e Forragens - Pastagens e Forragens em Pecuária Extensiva, 13-16 April 1999, Elvas, Portugal. pp.28 Goulão L, Cabrita L, Oliveira C and Leitão J (1998). Characterisation of apple (Malus x domestica Borkh.) cultivars by AFLP markers. Programa e Resumos do XI Congresso Nacional de Bioquímica, 28-30 November, Tomar, Portugal. pp.138 Monte-Corvo L, Mota M, Goulão L, Silva D and Oliveira C (1998). Taxonomic use of RAPD markers in Pyrus. Abstracts of the XXV International Horticultural Congress (IHC), 2-7 August 1998, Brussels, Belgium. pp.445 COMMUNICATIONS IN SCIENCE AND EDUCATION FOR DEVELOPMENT/COOPERATION AND IN DIVULGATION MEETINGS: Goulao LF (2013). Biotechnology towards a productive agriculture in challenging climatic environments, May 22-24, Maputo, Mozambique [Invited Oral Presentation by L. Goulao] Goulao LF (2013). Research progresses and challenges in biodiversity to food and nutritional security towards climate-smart agriculture in Mozambique. Policy Workshop on Climate Change Adaptation for Agriculture in Mozambique: Challenges for Agriculture Research, February 28, Maputo, Mozambique [Invited Oral Presentation by L. Goulao] Marques VR, Melo AMP and Goulão LF (2013). Riscos associados à pobreza e à transição nutricional em países da CPLP. Fórum Conselhos Científicos dos Laboratórios de Estado Ciclo de Encontros A Ciência na Prevenção e Mitigação dos Riscos em Portugal Riscos e Segurança Alimentar, January 31, Lisbon, Portugal [Oral Presentation by V. Marques] Goulao LF, Bila R, Mula HC, leitão AE, grundy I, Broli A, Mwangwela A, Kingsley M, Penicela L and Ribeiro AI (2012). Postharvest handling and conservation of indigenous fleshy fruits from southeastern Africa: first steps of the NutrIndiFruit project. Book of Abstracts of the International Congress Tropical Knowledge in Mozambique: history, memory and science, October 24-26, Lisbon, Portugal. pp. 65 [Oral Presentation by L. Goulao] Maquia I, Goulão LF, Ribeiro N and Ribeiro AI (2012). Caracterização molecular de recursos genéticos florestais das matas de miombo na Reserva Nacional de Niassa: desenvolvimento de códigos de barras e marcadores ISSR. Book of Abstracts of the International Congress Tropical Knowledge in Mozambique: history, memory and science, October 24-26, Lisbon, Portugal. pp. 68 [Oral Presentation by I. Maquia] Militão E, Maquia I, Mazivile M, Goulão L and Ribeiro AI (2012). Caracterização da diversidade genética da população de chanfuta (Afzelia quanzensis Welw.) da floresta de Michafutene. Book of Abstracts of the International Congress Tropical Knowledge in Mozambique: history, memory and science, October 24-26, Lisbon, Portugal. pp. 69 [Oral Presentation by E. Militão] Senkoro A, Barbosa F, Manjate A, Silva V, Maquia I, Muocha I, Moura I, Goulão L and Ribeiro A (2012). Estudo e conservação de plantas medicinais em Moçambique. Book of Abstracts of the International Congress Tropical Knowledge in Mozambique: history, memory and science, October 24-26, Lisbon, Portugal. pp. 62 [Oral Presentation by A. Senkoro] Ribeiro A, Muocha I, Maquia I, Goulão LF, Melo AMP, Senkoro A, Barbosa F, Ribeiro N, Saide J and Neves L (2012) Research & Education in Agrobiotechnology: a partnership between IICT & UEM. II Cooperation and Education (COOPEDU): Africa and the World, July 5-6, Lisbon, Portugal. pp. 15 [Oral Presentation by A. Ribeiro] Ribeiro A, Muocha I, Maquia I, Goulão LF, Melo AMP, Senkoro A, Barbosa F, Ribeiro N, Saide J and Neves L (2012). IICT (Portugal) & UEM (Mozambique): a consolidate partnership in agrobiotechnology. Programa & Resumos do Encontro Ensino Superior e Investigação Científica no Espaço da CPLP - XXII Encontro da Associação das Universidades de Língua Portuguesa (AULP), June 18-20, Maputo, Mozambique. pp. 54 [Oral Presentation by A. Ribeiro] 9

Ribeiro A and Goulao LF (2012). Biodiversidade e desenvolvimento sustentável. Cluster Tecnologias Tropicais : Biologia e Medicina Tropicais, AITECOeiras, May 29, Oeiras, Portugal [Invited Oral Presentation by L. Goulao] Lidon FC, Ramalho JC, Scotti-Campos P, Pais IP, Leitão AE, Almeida AS, Maçãs B, Coutinho JP, da Silva MMA, Duarte MP, Fernando AL, Ribeiro AI, Goulão LF, Simões F, Costa AR, Matos J, Reboredo FH (2012). The Portuguese project of wheat biofortification in Fe and Zn, COST Action FA0905. Programa e Resumos do Simpósio: Desenvolver culturas mais nutritivas Biofortificação de trigo, 6 March, Elvas, Portugal. pp. 13 [Presented by F. Lidon] (+ 4 supporting posters) Ribeiro A, Goulão LF, Melo AMP, Palos I and Ramalho J (2012). Colaboração interinstitucional para promoção da biotecnologia em Moçambique. IICT International Conference, Science in the Tropics: glimpsing the past, projecting the future, January 5-7, Lisboa, Portugal. pp. 25 [Oral Presentation by A. Ribeiro] Lidon FC, Leitão AE, Goulão LF, Ribeiro A and Ramalho JC (2012). Seriação de metodologias destinadas à produção de farinha de trigo biofortificada em Fe e Zn para populações carenciadas de países em vias de desenvolvimento. IICT International Conference, Science in the Tropics: glimpsing the past, projecting the future, January 5-7, Lisboa, Portugal. pp. 29 [Oral Presentation by F. Lidon] Ramalho JC, Leitão AE, Fortunato AS, Goulao LF, Melo AMP, Batista-Santos P, Santos P, Lopes E, Palos I, Lidon FC, Scotti-Campos P, Pais IP, Rodrigues AD, Jackson P, Lopes A, DaMatta FM and Ribeiro A (2012). Mecanismos de aclimatação a baixas temperaturas positivas em cafeeiro: Contribuição para a Investigação Agrícola para o Desenvolvimento no Contexto das Alterações Ambientais. IICT International Conference, Science in the Tropics: glimpsing the past, projecting the future, January 5-7, Lisboa, Portugal. pp. 56 [Poster] Silva MJ, Ferreira-Pinto MM, Fortunato A, Goulão L, Leitão AE, Leitão C, Santos MR, Ramalho JC and Ribeiro A (2012). Avaliação e caracterização de genótipos de purgueira. IICT International Conference, Science in the Tropics: glimpsing the past, projecting the future, January 5-7, Lisboa, Portugal. pp. 86 [Poster] Sousa PMF, Videira MAM, Silva STN, Carita J, Vaz F, Charro N, Penque D, Bohn A, Goulão LF, Hood BL, Conrads TP and Melo AMP (2011). Escherichia coli respiratory chain supercomplexes are present at the onset of aerobic growth. 2nd ITQB PhD Students Meeting: Book of Abstracts, October 26-28, Oeiras, Portugal. pp.54 [Poster] Rodrigues AD, Partelli FL, Ramalho JC, Palos IM, Fortunato AS and Goulao LF (2011). Uso do sistema antioxidativo como marcador de tolerância do cafeeiro arabica (Coffea arabica cv. Icatu) às baixas temperaturas positivas e secura. 37º Congresso Brasileiro de Pesquisas Cafeeiras - Mais tecnologia, mais café (Ed.: JB Matielo), Embrapa Café, November 1-4, Poços de Caldas, MG, Brazil. pp.289-290 (CD; ISSN 2176-3429) Ribeiro AI, Leitão AE, Silva MJ, Santos MR, Pinto MM, Domingues AM, Melo A, Goulão LF, Santos PC, Batista-Santos P, Fortunato AS, Graça IM, Silva S, Sousa P and Ramalho JC (2010). Agricultural Research for Development: A contribution of Eco-Bio/IICT. Livro de Actas do 1º Worshop Anual BioPlant, March 29-30, Porto, Portugal. pp.18 [Oral Presentation by A. Ribeiro] OTHER PUBLICATIONS: DIVULGATION PAPERS: Domingos S, Raposo AC, Ramalho JC, Oliveira CM and Goulao LF (2013). Efeito do ensombramento no vingamento e qualidade final em uva de mesa: Avaliação como método alternativo de monda de bagos. Revista da APH - Associação Portuguesa de Horticultura (in press). Oliveira C, Goulão L, Monte-Corvo L, Mota M, Nolasco G, Leitão J, Silva D and Sousa R (2000). Caracterização biomolecular de cultivares portuguesas de macieira e pereira: Defender o património genético. Investigação Agrária 1: 50-52. Goulão L, Hilário L, Fortunato M and Nunes P (1998). A importância dos bancos de germoplasma na manutenção da biodiversidade. Agros 81: 16-20. GENBANK (NCBI) SUBMISSIONS: 69 SEQUENCES (Corenucleotide): COFFEA ABIOTIC STRESS-RESPONSIVE PEROXIDASES: JQ013432- JQ013441 EUCALYPTUS CELL WALL-MODIFYING ENZYMES INVOLVED IN WOOD FORMATION: DQ100328-DQ100342, DQ376126-DQ376138, DQ645935-DQ645943 APPLE RIPENING-RELATED CELL WALL-MODIFYING ENZYMES: AF527800, AY144593, AY144594, AY309436, AY350734, AY376878, AY530907 TRANSCRIPTS DIFFERENTIALLY EXPRESSED DURING APPLE RIPENING: AY742293-AY742306 10

SCIENTIFIC ASSOCIATIONS MEMBERSHIP: International Society for Horticultural Science (ISHS; member no. 67561) Portuguese Biochemical Society / Portuguese Society of Plant Physiology (SPB; member no. 3274) Portuguese Society of Horticulture (Associação Portuguesa de Horticultura - APH; member no. 1954) Professional networks: International Tropical Fruits Network (TFNet) The Postharvest Education Foundation (TEF) IdCPLP: Rede de Investigação e Desenvolvimento na CPLP Plataforma de Biotecnologia de Plantas da CPLP Liga de Amigos do Jardim Botânico Tropical (LAJBT; member no. 70) INVOLVEMENT IN SCIENCE & SOCIETY ACTIVITIES: SINCE 2009 Scientific advisory on the implementation of a "Coffee Science Centre in Campo Maior (promoter: Delta Cafés/Grupo Nabeiro) 2013 High school students ( São João de Brito school) visit IICT (Talk: Research with Molecular Biology Globalization of nucleic acids) 2012 National Science & Technology Week - Ciência Viva program ( A Class in the Palace initiative - lecture: Food security for the tropics: our fruitful work) 2012 European Researchers Night (Organization of the activity: Biofuels alternatives at high speed) 2012 The first international "Fascination of Plants Day" - European Plant Science Organisation (EPSO): (Coorganization of the Oeiras Initiative Tropical Plants Tour ) 2011 National Science & Technology Week - Ciência Viva program (visit to high schools: Chatting with scientists: another way of learning) 2011 Participation in the event Coffee from the plant to the cup (Lecture: Coffee, the dark beverage of white dreams), Casino Figueira da Foz 2011 European Researchers Night (Co-organization of a Coffee-Science Debate: Tropical Synergies and a Peddy-Paper: Lost and Found in the Tropical Forest (promotional event)) 2010 European Researchers Night (Co-organization of a Peddy-Paper: Playing with Science) 2009 National Science & Technology Week - Ciência Viva program (Short lecture: Molecular Detectives) 2009 European Researchers Night ( Coffee-Science debate - talk: Molecular Agriculture: a look at the genes to improve the production) 2005 and 2008 Participation in the ITQB s Open Day Plant Division Stands updated: April 2013 11