(1) Os resultados marcados com foram obtidos com um número de resultados. inferior a 7.

CONTROLE INTERNO DE ESPECTRO Lote Validade ESP 129 Outubro / 2009 ESP 127 Outubro / 2009 Volume 7.0 ml ANVS 10086270001 CNPJ 29.511.607/0001-18 Rua Ana Neri, 416 Benfica Rio de Janeiro/RJ CEP 20911-442 Tel (21) 3891-9900 Fax (21) 3891-9901 atendimento@controllab.com.br www.controllab.com.br Resp. Técnica: Kátia O'Dwyer Nery CRF-RJ 6957 Armazenagem e Validade O espectro deve ser armazenado entre 2ºC e 8 o C. Durante o transporte, os materiais mantêm suas características a temperatura máxima de 30 o C, por 30 dias. Procedimento de Ensaio 1. Homogeneizar a amostra sem agitar; 2. Estabilizar o aparelho, mantendo-o ligado durante 15 minutos e escolher cubetas limpas e íntegras; 3. Utilizar uma cubeta com água reagente e outra com a amostra para ler as absorvâncias da solução contra a água, nos comprimentos de onda existentes neste documento e utilizados na rotina do laboratório; 4. Liberar os resultados conforme a análise dos resultados obtidos para o controle. Atenção Estes produtos utilizados para controle interno devem ser considerados como materiais que oferecem risco à saúde (3), devendo-se tomar as precauções habituais para a manipulação e o descarte segundo as normas de segurança. Resultados Esperados Os valores apresentados nesta bula foram obtidos através de Ensaio de Proficiência. Os dados individuais são agrupados de acordo com os parâmetros utilizados (Equipamento e ), e para cada grupo é apresentado a média depurada, com no mínimo 7 resultados (1), e o intervalo (calculado a partir dos limites apresentados abaixo). Valores utilizados para o cálculo do intervalo Absorvância 0,200 ± 30% Absorvância > 0,200 ± 20% As soluções possuem curvas espectrais distintas e complementares. Cada nível apresenta boa leitura (superior a 0,100) em um grupo de comprimentos de onda, para garantir a análise quantitativa (média, desvio padrão e coeficiente de variação) em toda a amplitude de comprimentos de onda em minimamente um nível. Leituras baixas (inferiores a 0,05) devem ser analisadas qualitativamente, conforme faixas apresentadas na bula. Para análise dos resultados o usuário pode, numa primeira fase, utilizar os intervalos fornecidos na bula ou calcular um novo intervalo. Em seguida é recomendado que o laboratório obtenha seus próprios valores (média e desvio padrão) e aplique as regras de Westgard (2) para análise do controle. (1) Os resultados marcados com foram obtidos com um número de resultados inferior a 7. (2) Artigos traduzidos do Westgard: acesse www.control-lab.com.br (3) Ver advertências e precauções no verso da desta página. Página 1 de 9

Advertências e Precauções Este produto é de origem humana e potencialmente infectante, portanto deve ser manuseado de acordo com as Boas Práticas de Biossegurança Padrão OSHA sobre Patógenos Transmitidos por Via Sangüínea. Todos os materiais de controle de qualidade devem ser tratados como potencialmente infectantes e deve-se seguir os procedimentos de biossegurança adotados pelo laboratório, incluindo: - luvas descartáveis; - vestiário de proteção; - equipamento protetor adequado para olhos/face; - ter um lava-olhos próximo ao local de manuseio da amostra. Cuidados: - evitar contato com a pele e olhos; - nunca pipetar pela boca; - não manusear lentes de contato no setor técnico; - não comer, beber, fumar ou aplicar cosméticos no setor técnico. Acidente Em caso de contato com a pele e olhos por meio de respingo ou corte, lavar imediatamente e abundantemente com água e sabão. Em seguida consulte um especialista. Bibliografia US Department of Labor, Occupational Safety and Health Administration, 29 CFR Part 1910. 1030, Occupational Exporue to Bloodborne Pathogens; Final Rule. Federal Register 1991:56 (235): 64175-82 US Department of Health and Human Services. Biosafety in Microbiological and Biomedical Laboratories. HHS Publication No. (CDC) 93-8395. Washington, DC: US Government Printing OFFICE, May 1999. World Health Organization.Laboratory Biosafety Manual.Geneva:World Health Organization, 1993. National Committee for Clinical Laboratory Standards. Protection odf Laboratory Workers from Occupationally Acquired Infectious; Approved Guideline Second Edition. NCCLS Document M29-A Wayne, PA:NCCLS, 2001. Página 2 de 9

340nm BioPlus Bio 200 Aspiração 10mm 0.245 0.196 0.294 0.178 0.125 0.231 BioPlus Bio 2000 Aspiração 10mm 0.245 0.195 0.294 0.181 0.127 0.235 BioPlus Bio 2000 Cubeta 10mm 0.256 0.205 0.308 0.181 0.127 0.235 BioPlus Bio 2000 Cubeta 12mm 0.274 0.218 0.329 0.192 0.134 0.250 BioSystems BTS 310 Aspiração 10mm 0.242 0.193 0.290 0.177 0.124 0.230 Celm E 205 D/ 210 D Cubeta 10mm 0.229 0.182 0.275 0.165 0.116 0.215 Celm E 215 D/ 225 D Cubeta 10mm 0.248 0.198 0.298 0.172 0.120 0.224 Drake Quick Lab Aspiração 10mm 0.206 0.164 0.248 0.147 0.103 0.191 Labtest Labquest Aspiração 10mm 0.234 0.187 0.281 0.175 0.123 0.228 Merck Microlab 100/ 200 Aspiração 10mm 0.265 0.212 0.319 0.183 0.128 0.238 Siemens/ Bayer RA50 Aspiração 10mm 0.266 0.212 0.319 0.188 0.132 0.244 Technicon RA 50 Aspiração 10mm 0.228 0.182 0.274 0.158 0.111 0.205 Technicon RA 50 Cubeta 10mm 0.259 0.207 0.311 0.178 0.125 0.231 Aspiração 10mm 0.234 0.187 0.282 0.170 0.119 0.221 Aspiração 12mm 0.238 0.190 0.286 0.169 0.118 0.220 Cubeta 10mm 0.248 0.198 0.298 0.173 0.121 0.225 Cubeta 12mm 0.266 0.212 0.320 0.188 0.132 0.244 Cubeta 8.5mm 0.301 0.241 0.362 0.195 0.137 0.254 400nm BL Spectronic 20/ 21 Cubeta 10mm 0.187 0.131 0.243 0.131 0.092 0.170 Celm E 205 D/ 210 D Cubeta 10mm 0.175 0.123 0.228 0.122 0.085 0.159 Celm E 205 D/ 210 D Cubeta 12mm 0.167 0.117 0.217 0.131 0.092 0.170 Celm E 215 D/ 225 D Cubeta 10mm 0.172 0.120 0.224 0.121 0.085 0.157 Celm E 215 D/ 225 D Cubeta 12mm 0.177 0.124 0.230 0.114 0.080 0.148 Aspiração 10mm 0.149 0.104 0.194 0.104 0.073 0.135 Cubeta 10mm 0.173 0.121 0.225 0.122 0.085 0.159 Cubeta 12mm 0.172 0.120 0.224 0.124 0.087 0.161 405nm BioPlus Bio 200 Aspiração 10mm 0.149 0.104 0.194 0.112 0.078 0.146 BioPlus Bio 2000 Aspiração 10mm 0.160 0.112 0.208 0.116 0.081 0.151 BioPlus Bio 2000 Cubeta 10mm 0.167 0.117 0.217 0.116 0.081 0.151 BioPlus Bio 2000 Cubeta 12mm 0.170 0.119 0.221 0.121 0.085 0.157 BioSystems BTS 310 Aspiração 10mm 0.149 0.104 0.194 0.108 0.076 0.140 BL Spectronic 20/ 21 Cubeta 10mm 0.190 0.133 0.247 0.131 0.092 0.170 Celm E 205 D/ 210 D Cubeta 10mm 0.180 0.126 0.234 0.129 0.090 0.168 Celm E 205 D/ 210 D Cubeta 12mm 0.165 0.116 0.215 0.122 0.085 0.159 Celm E 215 D/ 225 D Cubeta 10mm 0.176 0.123 0.229 0.124 0.087 0.161 Celm SB 190 Aspiração 10mm 0.145 0.102 0.189 0.105 0.074 0.137 Drake Quick Lab Aspiração 10mm 0.144 0.101 0.187 0.105 0.074 0.137 Labtest Labquest Aspiração 10mm 0.152 0.106 0.198 0.114 0.080 0.148 Technicon RA 50 Aspiração 10mm 0.153 0.107 0.199 0.111 0.078 0.144 Aspiração 10mm 0.151 0.106 0.196 0.111 0.078 0.144 Aspiração 12mm 0.147 0.103 0.191 0.107 0.075 0.139 Cubeta 10mm 0.174 0.122 0.226 0.122 0.085 0.159 Cubeta 12mm 0.170 0.119 0.221 0.121 0.085 0.157 Cubeta 8.5mm 0.170 0.119 0.221 0.118 0.083 0.153 Página 3 de 9

420nm BioSystems BTS 310 Aspiração 10mm 0.161 0.113 0.209 0.114 0.080 0.148 BioSystems BTS 310 Aspiração 12mm 0.157 0.110 0.204 0.109 0.076 0.142 BL Spectronic 20/ 21 Cubeta 10mm 0.207 0.145 0.269 0.144 0.101 0.187 Celm E 205 D/ 210 D Cubeta 10mm 0.195 0.137 0.254 0.136 0.095 0.177 Celm E 205 D/ 210 D Cubeta 12mm 0.189 0.132 0.246 0.131 0.092 0.170 Celm E 215 D/ 225 D Cubeta 10mm 0.192 0.134 0.250 0.135 0.095 0.176 Aspiração 10mm 0.167 0.117 0.217 0.119 0.083 0.155 Aspiração 12mm 0.157 0.110 0.204 0.110 0.077 0.143 Cubeta 10mm 0.192 0.134 0.250 0.134 0.094 0.174 Cubeta 12mm 0.185 0.130 0.241 0.129 0.090 0.168 Cubeta 8.5mm 0.180 0.126 0.234 0.126 0.088 0.164 450nm BioPlus Bio 2000 Aspiração 10mm 0.241 0.192 0.290 0.177 0.124 0.230 BioPlus Bio 2000 Cubeta 10mm 0.261 0.208 0.314 0.183 0.128 0.238 BL Spectronic 20/ 21 Cubeta 10mm 0.291 0.232 0.349 0.207 0.145 0.269 Celm E 205 D/ 210 D Cubeta 10mm 0.282 0.225 0.339 0.198 0.139 0.257 Celm E 205 D/ 210 D Cubeta 12mm 0.267 0.213 0.321 0.189 0.132 0.246 Celm E 215 D/ 225 D Cubeta 10mm 0.280 0.224 0.336 0.196 0.137 0.255 Aspiração 10mm 0.250 0.199 0.300 0.181 0.127 0.235 Aspiração 12mm 0.262 0.209 0.315 0.189 0.132 0.246 Cubeta 10mm 0.276 0.220 0.331 0.193 0.135 0.251 Cubeta 12mm 0.269 0.215 0.324 0.191 0.134 0.248 460nm BL Spectronic 20/ 21 Cubeta 10mm 0.361 0.288 0.433 0.250 0.200 0.301 BL Spectronic 70/ 88 Cubeta 10mm 0.378 0.302 0.454 0.258 0.206 0.310 Celm E 205 D/ 210 D Cubeta 10mm 0.339 0.271 0.408 0.240 0.191 0.288 Celm E 205 D/ 210 D Cubeta 12mm 0.324 0.258 0.389 0.234 0.187 0.282 Celm E 215 D/ 225 D Cubeta 10mm 0.350 0.279 0.420 0.246 0.196 0.295 Aspiração 10mm 0.295 0.235 0.354 0.209 0.167 0.252 Cubeta 10mm 0.341 0.272 0.409 0.240 0.192 0.288 Cubeta 12mm 0.323 0.258 0.388 0.230 0.184 0.277 475nm BL Spectronic 20/ 21 Cubeta 10mm 0.497 0.397 0.597 0.350 0.279 0.420 BL Spectronic 70/ 88 Cubeta 10mm 0.516 0.413 0.620 0.353 0.282 0.424 Celm E 205 D/ 210 D Cubeta 10mm 0.473 0.378 0.568 0.331 0.265 0.398 Celm E 215 D/ 225 D Cubeta 10mm 0.489 0.391 0.587 0.346 0.276 0.415 Celm E 215 D/ 225 D Cubeta 12mm 0.483 0.386 0.581 0.331 0.265 0.398 Aspiração 10mm 0.417 0.333 0.501 0.303 0.242 0.365 Cubeta 10mm 0.478 0.382 0.573 0.336 0.268 0.404 Cubeta 12mm 0.453 0.362 0.544 0.323 0.258 0.389 Página 4 de 9

500nm BL Spectronic 20/ 21 Cubeta 10mm 0.747 0.597 0.897 0.509 0.407 0.611 BL Spectronic 70/ 88 Cubeta 10mm 0.751 0.600 0.902 0.531 0.424 0.638 Celm E 205 D/ 210 D Cubeta 10mm 0.714 0.570 0.857 0.509 0.406 0.611 Celm E 205 D/ 210 D Cubeta 12mm 0.697 0.557 0.837 0.497 0.397 0.597 Celm E 215 D/ 225 D Cubeta 10mm 0.746 0.596 0.895 0.521 0.416 0.625 Celm E 215 D/ 225 D Cubeta 12mm 0.743 0.594 0.892 0.511 0.408 0.613 Drake Quick Lab Aspiração 10mm 0.626 0.501 0.752 0.466 0.372 0.559 Siemens/ Bayer RA50 Aspiração 10mm 0.687 0.549 0.825 0.491 0.392 0.590 Technicon RA 50 Aspiração 10mm 0.658 0.526 0.790 0.475 0.380 0.571 Technicon RA 50 Cubeta 10mm 0.694 0.555 0.833 0.493 0.394 0.592 Aspiração 10mm 0.637 0.509 0.765 0.464 0.371 0.557 Aspiração 12mm 0.638 0.510 0.766 0.446 0.357 0.536 Cubeta 10mm 0.722 0.577 0.867 0.508 0.406 0.610 Cubeta 12mm 0.695 0.555 0.834 0.493 0.394 0.592 505nm BioPlus Bio 200 Aspiração 10mm 0.676 0.540 0.812 0.492 0.393 0.591 BioPlus Bio 2000 Aspiração 10mm 0.673 0.538 0.808 0.498 0.398 0.598 BioPlus Bio 2000 Cubeta 10mm 0.736 0.588 0.883 0.519 0.415 0.624 BioPlus Bio 2000 Cubeta 12mm 0.770 0.616 0.924 0.539 0.430 0.647 BioSystems BTS 310 Aspiração 10mm 0.652 0.521 0.783 0.466 0.373 0.560 BL Spectronic 20/ 21 Cubeta 10mm 0.770 0.615 0.924 0.530 0.423 0.636 Celm E 205 D/ 210 D Cubeta 10mm 0.752 0.601 0.903 0.534 0.427 0.642 Celm E 205 D/ 210 D Cubeta 12mm 0.746 0.596 0.895 0.526 0.420 0.632 Celm E 215 D/ 225 D Cubeta 10mm 0.778 0.622 0.935 0.544 0.435 0.653 Celm E 215 D/ 225 D Cubeta 12mm 0.779 0.622 0.935 0.534 0.427 0.641 Celm SB 190 Aspiração 10mm 0.634 0.507 0.761 0.458 0.366 0.550 Labtest Labquest Aspiração 10mm 0.668 0.534 0.802 0.495 0.396 0.595 Merck Microlab 100/ 200 Aspiração 10mm 0.698 0.558 0.838 0.496 0.397 0.596 Micronal 280/380/382/582 Cubeta 10mm 0.658 0.526 0.790 0.521 0.416 0.626 Aspiração 10mm 0.669 0.535 0.804 0.484 0.386 0.581 Aspiração 12mm 0.666 0.533 0.800 0.485 0.388 0.583 Cubeta 10mm 0.750 0.600 0.901 0.528 0.422 0.634 Cubeta 12mm 0.749 0.599 0.900 0.525 0.419 0.630 Cubeta 8.5mm 0.639 0.511 0.767 0.461 0.368 0.553 510nm BL Spectronic 20/ 21 Cubeta 10mm 0.798 0.638 0.958 0.550 0.440 0.661 BL Spectronic 70/ 88 Cubeta 10mm 0.813 0.650 0.976 0.575 0.459 0.690 Celm E 205 D/ 210 D Cubeta 10mm 0.780 0.624 0.937 0.551 0.440 0.661 Celm E 205 D/ 210 D Cubeta 12mm 0.776 0.621 0.932 0.539 0.431 0.647 Celm E 215 D/ 225 D Cubeta 10mm 0.806 0.644 0.968 0.565 0.452 0.679 Celm E 215 D/ 225 D Cubeta 12mm 0.800 0.640 0.961 0.548 0.438 0.658 Aspiração 10mm 0.676 0.540 0.812 0.466 0.372 0.559 Cubeta 10mm 0.789 0.630 0.947 0.555 0.443 0.666 Cubeta 12mm 0.780 0.623 0.936 0.543 0.434 0.652 Cubeta 8.5mm 0.685 0.548 0.823 0.498 0.398 0.598 Página 5 de 9

520nm BL Spectronic 20/ 21 Cubeta 10mm 0.807 0.645 0.969 0.567 0.453 0.681 Celm E 205 D/ 210 D Cubeta 10mm 0.809 0.647 0.971 0.570 0.456 0.685 Celm E 205 D/ 210 D Cubeta 12mm 0.797 0.637 0.957 0.566 0.452 0.680 Celm E 215 D/ 225 D Cubeta 10mm 0.827 0.661 0.993 0.579 0.463 0.695 Celm E 215 D/ 225 D Cubeta 12mm 0.833 0.666 1.000 0.575 0.459 0.690 Aspiração 10mm 0.724 0.578 0.869 0.505 0.403 0.606 Cubeta 10mm 0.810 0.648 0.973 0.570 0.456 0.685 Cubeta 12mm 0.799 0.639 0.959 0.563 0.450 0.675 Cubeta 8.5mm 0.725 0.580 0.870 0.527 0.421 0.633 530nm BL Spectronic 20/ 21 Cubeta 10mm 0.774 0.618 0.929 0.543 0.434 0.651 BL Spectronic 70/ 88 Cubeta 10mm 0.829 0.663 0.996 0.597 0.477 0.716 Celm E 205 D/ 210 D Cubeta 10mm 0.787 0.629 0.944 0.552 0.441 0.663 Celm E 205 D/ 210 D Cubeta 12mm 0.774 0.619 0.929 0.545 0.436 0.654 Celm E 215 D/ 225 D Cubeta 10mm 0.799 0.638 0.959 0.562 0.449 0.675 Celm E 215 D/ 225 D Cubeta 12mm 0.820 0.655 0.984 0.563 0.450 0.676 Drake Quick Lab Aspiração 10mm 0.613 0.490 0.736 0.444 0.354 0.533 Aspiração 10mm 0.649 0.519 0.779 0.467 0.373 0.561 Cubeta 10mm 0.786 0.628 0.944 0.554 0.443 0.666 Cubeta 12mm 0.780 0.623 0.936 0.547 0.437 0.657 Cubeta 8.5mm 0.717 0.573 0.861 0.524 0.419 0.629 546nm BioPlus Bio 200 Aspiração 10mm 0.601 0.481 0.722 0.441 0.352 0.530 BioPlus Bio 2000 Aspiração 10mm 0.591 0.473 0.710 0.439 0.351 0.528 BioPlus Bio 2000 Cubeta 10mm 0.664 0.531 0.798 0.465 0.371 0.558 BioPlus Bio 2000 Cubeta 12mm 0.683 0.546 0.820 0.479 0.382 0.575 BioSystems BTS 310 Aspiração 10mm 0.560 0.447 0.672 0.399 0.318 0.479 BL Spectronic 20/ 21 Cubeta 10mm 0.673 0.538 0.808 0.460 0.367 0.552 Celm E 205 D/ 210 D Cubeta 10mm 0.675 0.540 0.810 0.477 0.381 0.573 Celm E 205 D/ 210 D Cubeta 12mm 0.655 0.523 0.786 0.470 0.375 0.564 Celm E 215 D/ 225 D Cubeta 10mm 0.682 0.545 0.819 0.479 0.382 0.575 Celm E 215 D/ 225 D Cubeta 12mm 0.709 0.567 0.851 0.494 0.394 0.593 Drake Quick Lab Aspiração 10mm 0.580 0.463 0.696 0.422 0.337 0.507 Labtest Labquest Aspiração 10mm 0.592 0.473 0.711 0.440 0.351 0.528 Micronal 280/380/382/582 Cubeta 10mm 0.588 0.470 0.706 0.456 0.364 0.548 Micronal 342/442 Cubeta 10mm 0.612 0.489 0.735 0.414 0.331 0.498 Siemens/ Bayer RA50 Aspiração 10mm 0.633 0.506 0.760 0.462 0.369 0.555 Technicon RA 50 Aspiração 10mm 0.605 0.484 0.726 0.442 0.353 0.531 Technicon RA 50 Cubeta 10mm 0.640 0.511 0.768 0.448 0.358 0.538 Aspiração 10mm 0.595 0.475 0.714 0.434 0.346 0.521 Aspiração 12mm 0.581 0.464 0.698 0.433 0.346 0.520 Cubeta 10mm 0.661 0.528 0.794 0.467 0.373 0.561 Cubeta 12mm 0.669 0.535 0.804 0.473 0.378 0.568 Cubeta 8.5mm 0.661 0.528 0.794 0.461 0.369 0.554 Página 6 de 9

550nm Celm E 205 D/ 210 D Cubeta 10mm 0.639 0.511 0.768 0.448 0.358 0.538 Celm E 205 D/ 210 D Cubeta 12mm 0.617 0.493 0.741 0.439 0.350 0.527 Celm E 215 D/ 225 D Cubeta 10mm 0.637 0.509 0.765 0.450 0.359 0.540 Celm E 215 D/ 225 D Cubeta 12mm 0.667 0.533 0.800 0.461 0.368 0.554 Aspiração 10mm 0.589 0.470 0.707 0.457 0.365 0.549 Cubeta 10mm 0.631 0.504 0.757 0.444 0.355 0.533 Cubeta 12mm 0.635 0.507 0.762 0.448 0.358 0.538 560nm BL Spectronic 20/ 21 Cubeta 10mm 0.526 0.420 0.632 0.353 0.282 0.423 Celm E 205 D/ 210 D Cubeta 10mm 0.530 0.423 0.636 0.370 0.296 0.445 Celm E 205 D/ 210 D Cubeta 12mm 0.517 0.413 0.621 0.363 0.290 0.435 Celm E 215 D/ 225 D Cubeta 10mm 0.524 0.419 0.629 0.366 0.292 0.439 Aspiração 10mm 0.525 0.420 0.631 0.391 0.313 0.470 Cubeta 10mm 0.520 0.415 0.624 0.364 0.291 0.437 Cubeta 12mm 0.531 0.424 0.638 0.370 0.296 0.445 Cubeta 8.5mm 0.539 0.430 0.647 0.386 0.308 0.463 578nm BioPlus Bio 200 Aspiração 10mm 0.248 0.198 0.297 0.177 0.124 0.230 BioPlus Bio 2000 Aspiração 10mm 0.266 0.212 0.320 0.187 0.131 0.243 BioPlus Bio 2000 Cubeta 10mm 0.277 0.221 0.333 0.194 0.136 0.252 BioPlus Bio 2000 Cubeta 12mm 0.277 0.221 0.333 0.191 0.134 0.248 BioSystems BTS 310 Aspiração 10mm 0.243 0.194 0.292 0.173 0.121 0.225 BioSystems BTS 310 Cubeta 12mm 0.252 0.201 0.303 0.177 0.124 0.230 Celm E 205 D/ 210 D Cubeta 10mm 0.309 0.247 0.372 0.215 0.151 0.280 Celm E 205 D/ 210 D Cubeta 12mm 0.305 0.243 0.366 0.216 0.151 0.281 Celm E 215 D/ 225 D Cubeta 10mm 0.308 0.246 0.370 0.211 0.148 0.274 Celm E 215 D/ 225 D Cubeta 12mm 0.315 0.252 0.379 0.215 0.151 0.280 Labtest Labquest Aspiração 10mm 0.248 0.198 0.298 0.179 0.125 0.233 Merck Microlab 100/ 200 Aspiração 10mm 0.266 0.213 0.320 0.179 0.125 0.233 Micronal 342/442 Cubeta 10mm 0.330 0.264 0.397 0.246 0.172 0.320 Siemens/ Bayer RA50 Aspiração 10mm 0.265 0.211 0.318 0.183 0.128 0.238 Technicon RA 50 Aspiração 10mm 0.249 0.199 0.299 0.176 0.123 0.229 Aspiração 10mm 0.262 0.209 0.315 0.185 0.130 0.241 Aspiração 12mm 0.227 0.181 0.272 0.160 0.112 0.208 Cubeta 10mm 0.293 0.234 0.352 0.203 0.142 0.264 Cubeta 12mm 0.293 0.234 0.352 0.204 0.143 0.265 Cubeta 8.5mm 0.301 0.241 0.362 0.220 0.154 0.286 580nm Celm E 205 D/ 210 D Cubeta 10mm 0.285 0.227 0.342 0.197 0.138 0.256 Celm E 205 D/ 210 D Cubeta 12mm 0.270 0.215 0.324 0.192 0.134 0.250 Celm E 215 D/ 225 D Cubeta 10mm 0.280 0.224 0.337 0.193 0.135 0.251 Celm E 215 D/ 225 D Cubeta 12mm 0.292 0.233 0.351 0.201 0.141 0.261 Drake Quick Lab Aspiração 10mm 0.269 0.215 0.324 0.193 0.135 0.251 Micronal 342/442 Cubeta 10mm 0.259 0.207 0.311 0.195 0.137 0.254 Aspiração 10mm 0.281 0.224 0.338 0.205 0.144 0.267 Aspiração 12mm 0.280 0.224 0.336 0.219 0.153 0.285 Cubeta 10mm 0.277 0.221 0.333 0.193 0.135 0.251 Cubeta 12mm 0.279 0.223 0.335 0.195 0.137 0.254 Página 7 de 9

590nm BL Spectronic 20/ 21 Cubeta 10mm 0.181 0.127 0.235 0.120 0.084 0.156 Celm E 205 D/ 210 D Cubeta 10mm 0.190 0.133 0.247 0.127 0.089 0.165 Celm E 205 D/ 210 D Cubeta 12mm 0.179 0.125 0.233 0.121 0.085 0.157 Celm E 215 D/ 225 D Cubeta 10mm 0.188 0.132 0.244 0.128 0.090 0.166 Celm E 215 D/ 225 D Cubeta 12mm 0.195 0.137 0.254 0.132 0.092 0.172 Aspiração 10mm 0.233 0.163 0.303 0.159 0.111 0.207 Cubeta 10mm 0.187 0.131 0.243 0.128 0.090 0.166 Cubeta 12mm 0.186 0.130 0.242 0.125 0.088 0.163 600nm BL Spectronic 20/ 21 Cubeta 10mm 0.144 0.100 0.187 0.095 0.061 0.123 Celm E 205 D/ 210 D Cubeta 10mm 0.143 0.100 0.186 0.098 0.064 0.127 Celm E 205 D/ 210 D Cubeta 12mm 0.137 0.096 0.177 0.096 0.063 0.124 Celm E 215 D/ 225 D Cubeta 10mm 0.141 0.098 0.183 0.096 0.062 0.125 Celm E 215 D/ 225 D Cubeta 12mm 0.144 0.101 0.188 0.097 0.063 0.126 Aspiração 10mm 0.146 0.102 0.189 0.101 0.066 0.131 Cubeta 10mm 0.140 0.098 0.181 0.097 0.063 0.126 Cubeta 12mm 0.142 0.099 0.184 0.098 0.064 0.127 620nm BioPlus Bio 200 Aspiração 10mm 0.134 0.094 0.175 0.097 0.068 0.126 BioPlus Bio 2000 Aspiração 10mm 0.138 0.096 0.179 0.098 0.069 0.127 BioPlus Bio 2000 Cubeta 10mm 0.133 0.093 0.173 0.100 0.070 0.130 BioPlus Bio 2000 Cubeta 12mm 0.142 0.100 0.185 0.096 0.067 0.125 BL Spectronic 20/ 21 Cubeta 10mm 0.154 0.108 0.200 0.113 0.079 0.147 Celm E 205 D/ 210 D Aspiração 10mm 0.126 0.088 0.164 0.092 0.064 0.120 Celm E 205 D/ 210 D Cubeta 10mm 0.147 0.103 0.191 0.103 0.072 0.134 Celm E 205 D/ 210 D Cubeta 12mm 0.140 0.098 0.182 0.099 0.069 0.129 Celm E 215 D/ 225 D Cubeta 10mm 0.145 0.101 0.188 0.100 0.070 0.130 Celm E 215 D/ 225 D Cubeta 12mm 0.141 0.098 0.183 0.096 0.067 0.125 Drake Quick Lab Aspiração 10mm 0.131 0.092 0.171 0.094 0.066 0.122 Labtest Labquest Aspiração 10mm 0.152 0.106 0.198 0.107 0.075 0.139 Micronal 280/380/382/582 Cubeta 10mm 0.132 0.092 0.172 0.097 0.068 0.126 Quibasa/ Bioclin System / II Aspiração 10mm 0.137 0.096 0.178 0.099 0.069 0.129 Siemens/ Bayer RA50 Aspiração 10mm 0.138 0.096 0.179 0.098 0.069 0.127 Technicon RA 50 Aspiração 10mm 0.130 0.091 0.169 0.092 0.064 0.120 Technicon RA 50 Cubeta 10mm 0.143 0.100 0.186 0.103 0.072 0.134 Aspiração 10mm 0.136 0.095 0.177 0.097 0.068 0.126 Aspiração 12mm 0.130 0.091 0.169 0.095 0.067 0.124 Cubeta 10mm 0.144 0.101 0.187 0.102 0.071 0.133 Cubeta 12mm 0.141 0.099 0.183 0.097 0.068 0.126 635nm BL Spectronic 20/ 21 Cubeta 10mm 0.210 0.167 0.252 0.157 0.110 0.204 Celm E 205 D/ 210 D Cubeta 10mm 0.191 0.152 0.230 0.135 0.095 0.176 Celm E 205 D/ 210 D Cubeta 12mm 0.187 0.149 0.225 0.137 0.096 0.178 Celm E 215 D/ 225 D Cubeta 10mm 0.192 0.153 0.230 0.134 0.094 0.174 Celm E 215 D/ 225 D Cubeta 12mm 0.180 0.143 0.216 0.127 0.089 0.165 Aspiração 10mm 0.179 0.125 0.233 0.139 0.097 0.181 Cubeta 10mm 0.192 0.134 0.250 0.136 0.095 0.177 Cubeta 12mm 0.183 0.128 0.238 0.132 0.092 0.172 Página 8 de 9

670nm BioPlus Bio 200 Aspiração 10mm 0.363 0.254 0.473 0.264 0.184 0.343 BioPlus Bio 200 Cubeta 10mm 0.360 0.252 0.469 0.256 0.179 0.334 BioPlus Bio 2000 Aspiração 10mm 0.376 0.262 0.489 0.267 0.186 0.347 BioPlus Bio 2000 Cubeta 10mm 0.382 0.267 0.497 0.270 0.188 0.351 BioPlus Bio 2000 Cubeta 12mm 0.382 0.267 0.497 0.269 0.187 0.350 BioSystems BTS 310 Aspiração 10mm 0.357 0.250 0.465 0.254 0.177 0.331 BioSystems BTS 310 Aspiração 12mm 0.338 0.236 0.440 0.239 0.167 0.311 BioSystems BTS 310 Cubeta 10mm 0.374 0.261 0.486 0.267 0.186 0.347 BioSystems BTS 310 Cubeta 12mm 0.371 0.259 0.483 0.262 0.183 0.341 Celm E 205 D/ 210 D Cubeta 10mm 0.380 0.266 0.495 0.267 0.187 0.348 Celm E 215 D/ 225 D Cubeta 10mm 0.379 0.265 0.493 0.265 0.185 0.344 Celm E 215 D/ 225 D Cubeta 12mm 0.357 0.249 0.465 0.254 0.177 0.330 Drake Quick Lab Aspiração 10mm 0.344 0.240 0.447 0.249 0.174 0.324 Labtest Labquest Aspiração 10mm 0.366 0.256 0.476 0.260 0.182 0.339 Micronal 280/380/382/582 Cubeta 10mm 0.356 0.248 0.463 0.258 0.180 0.336 Quibasa/ Bioclin System / II Aspiração 10mm 0.364 0.254 0.474 0.252 0.176 0.328 Siemens/ Bayer RA50 Aspiração 10mm 0.357 0.249 0.464 0.247 0.172 0.322 Technicon RA 50 Aspiração 10mm 0.348 0.243 0.453 0.246 0.172 0.320 Technicon RA 50 Cubeta 10mm 0.366 0.256 0.476 0.255 0.178 0.332 Aspiração 10mm 0.362 0.253 0.471 0.258 0.180 0.336 Aspiração 12mm 0.348 0.243 0.453 0.253 0.177 0.330 Cubeta 10mm 0.377 0.264 0.491 0.266 0.186 0.347 Cubeta 12mm 0.367 0.257 0.478 0.262 0.183 0.341 Cubeta 8.5mm 0.358 0.250 0.466 0.247 0.172 0.321 700nm BL Spectronic 20/ 21 Cubeta 10mm 0.597 0.477 0.717 0.412 0.329 0.495 Celm E 205 D/ 210 D Cubeta 10mm 0.572 0.457 0.686 0.399 0.319 0.479 Celm E 205 D/ 210 D Cubeta 12mm 0.540 0.431 0.648 0.397 0.317 0.476 Celm E 215 D/ 225 D Cubeta 10mm 0.573 0.458 0.688 0.399 0.319 0.480 Celm E 215 D/ 225 D Cubeta 12mm 0.557 0.445 0.669 0.384 0.307 0.462 Aspiração 10mm 0.534 0.426 0.641 0.358 0.286 0.430 Cubeta 10mm 0.572 0.457 0.687 0.401 0.320 0.482 Cubeta 12mm 0.556 0.444 0.668 0.396 0.316 0.476 Página 9 de 9