XLII Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology (SBBq) Program. And. Index

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2 XLII Annual Meeting of the Brazilian Society for Biochemistry and Molecular Biology (SBBq) Program And Index Convention Center of Rafain Palace Hotel Foz do Iguaçu, Paraná, Brazil May 18 th to 21 st, 2013

3 Copyright 2013 Sociedade Brasileira de Bioquímica e Biologia Molecular (SBBq) All abstracts published in this book were reproduced from texts supplied by their authors. The content of these abstracts is the responsibility of these authors. SBBq, its directors, staff and ad hoc reviewers are not responsible for the consequences of the use of data published in this book.. Ilustração da Capa: Alexandre T. Bando

4 Welcome Message Dear Colleagues, On behalf of the entire organizing committee, we welcome the participants of the XLII Annual Meeting of the Brazilian Biochemistry and Molecular Biology Society (SBBq) in Foz do Iguaçu, Paraná, Brazil. The Congress aims to offer a broad overview of the frontiers of research and recent developments in many aspects of Biochemistry and Molecular Biology in the Americas and the Iberian Peninsula. The Annual Meeting of the Brazilian Biochemistry and Molecular Biology Society (SBBq) is currently one of the most traditional events of the Brazilian scientific community. For over four decades, these meetings have provided the scenario and forum for debates concerning the advancement of fundamental knowledge of Biochemical phenomena, their employment for social benefit and integration of this knowledge with other segments of the biological and physiochemical sciences. We wish to thank all the highly accomplished invited speakers for accepting our invitation to present their work at the Congress, thus contributing to a program that we trust all participants will find interesting. We also wish to welcome all the participants who come from abroad, hoping that they will find time to experience some of our culture, as well as to discuss Science with us. Bem-vindos ao Brasil!!! Moacir Wajner President of SBBq Carlos Termingoni Vice President of SBBq

5 Financial Support We gratefully acknowledge our main sponsors Financial Support also provide by INCT - Fixação Biológica de Nitrogênio

6 Board of Directors SBBq Executive Committee Acting President: Moacir Wajner (UFRGS) Vice President: Carlos Termignoni (UFRGS) Past President Helena C.F. Oliveira (UNICAMP) General Secretary: Walter R. Terra (USP-IQ) First Secretary: Sandro R. Marana (USP-IQ) Treasurer: Alicia J. Kowaltowski (USP-IQ) First Treasurer: Maurício S. Baptista (USP-IQ) Scientific Council Carlos Bloch Jr (Embraba-Cenargen) Hernan Francisco Terenzi (UFSC) Leda Quercia Vieira (UFMG) Maria Aparecida Juliano (UNIFESP) Maria Julia Manso Alves (USP-IQ) Pedro Lagerblad De Oliveira (UFRJ-IBqM) Richard Charles Garrat (USP-IFSC) Selma Jeronimo (UFRN) Regional Representatives Bahia Ceará Distrito Federal Minas Gerais Paraná Pernambuco Rio de Janeiro Rio Grande do Norte Rio Grande do Sul Maria de Fátima Dias Costa (UFBa) Renato de Azevedo Moreira (UFC) Marcelo Valle de Souza (UnB) Marcelo Matos Santoro (UFMG) Marcello Iacomini (UFPR) Vera Lúcia Menezes de Lima (UFPE) Hatisaburo Masuda (UFRJ) Hugo Alexandre de Oliveira Rocha (UFRN) Carlos Alberto Saraiva Gonçalves (UFRGS) Executive Secretariat Cynthia Sayuri Bando Arnaldo Casari Marcelo Andrade 5

7 General Information Badges SBBq staff will provide badges for all delegates and accompanying persons. Access to scientific sessions and other activities, including social events, will be denied unless badges are used. Badges contribute greatly to promote personal contacts and also guarantee the full attention of the Congress Personnel, as well as security control. Upon losing the badge, a duplicate will be provided at the Secretariat at cost of US$ 25,00. Registration Desk: The Registration Desk available for information and distribution of badges, bags and documents for financial support. Foyer. Saturday May 18 th 02:00 pm to 08:00 pm Sunday May 19 th 08:00 am to 13:00 pm Application for Financial Support: (only for Brazilian participants) Tuesday May 22 nd Medical Service: During the event there is an intensive-care unit in front of the Rafain Expocenter Complex and a Medical Service at Parana Room V, with 2 doctors and 1 nurse which will be available for emergency first aid. Saturday May 18 th 08:00 pm to 7:00 pm Sunday May 19 th 08:00 am to 7:00 pm Monday May 20 th 08:00 am to 7:00 pm Tuesday May 21 st 08:00 am to 7:00 pm Information for Speakers: The Multimedia Desk Room is located on Parana Room I, near on SBBq Secretariat and is open during the following hours: Saturday May 18 th 9:00 am to 7:00 pm Sunday May 19 th 8:00 am to 7:00 pm Monday May 20 th 8:00 am to 7:00 pm Tuesday May 21 st 8:00 am to 4:00 pm Speakers Room: Paraná Room Saturday May 18 th 9:00 am to 6:30 pm Sunday May 19 th 8:00 am to 6:30 pm Monday May 20 th 8:00 am to 6:30 pm Tuesday May 21 st 8:00 am to 6:30 pm 6

8 Poster Session: Expocenter I, II and III Presentation Sunday May 19 th 4:30 am to 6:30 pm Monday May 20 th 4:30 am to 6:30 pm Tuesday May 21 st 4:30 am to 6:30 pm All Pôster MUST BE fixed on Saturday (may 18th) until 6 pm Technical, Institutional and Books Exhibits: Expocenter I and II and Foyer Saturday May 18 th 02:00 pm to 7:00 pm Sunday May 19 th 10:00 am to 7:00 pm Monday May 20 th 10:00 am to 7:00 pm Tuesday May 21 st 10:00 am to 7:00 pm SBBq Secretariat and Administration Office: Paraná II Saturday May 18 th 2:00 pm to 7:00 pm Sunday May 19 th 8:00 am to 6:30 pm Monday May 20 th 8:00 am to 6:30 pm Tuesday May 21 st 8:00 am to 7:00 pm SBBq Awards Ceremony The announcement of SBBq Award for the best poster presentation and XVII Prize for Young Talent in Life Sciences will be on the Closing Ceremony on Tuesday, May 21 st, at 6:45 pm Local: Expocenter IV Meeting of the Board of Council Will be on Sunday, May 19 th, at 6 pm Local: Parana Room General Assembly Will be on Monday, May 20 tt, at 6 pm Local: Expocenter IV Room C 7

9 Saturday May 18 th, 2013 Expocenter IV XLII Annual SBBq Meeting - Scientific Program a Foz do Iguaçu, Paraná 5:30 Opening Session 6:00 pm Opening Conference Chair: Moacir Wajner, Univ Federal do Rio Grande do Sul, SBBq President 6:00 7:00 pm Jerson Lima da Silva, Univ Federal do Rio de Janeiro Is Cancer a Prion Disease? Amyloidogenic and Prion-Like Properties of Mutant p53 Sunday - May 19 th, :30-10:30 am Symposium 1 Novel Insights Over Peptides for Drug Development Chair: Octávio L.Franco, Univ Católica de Brasília Yeshiel Shai, Weizmann Institute Science, Israel Host Defense Antimicrobial and Antifungal Peptides and Lipopeptides and Potential Bacterial Resistance against Them Expo IV A Octávio L. Franco, Univ Católica de Brasília The Challenges of Sepsis Control by Using Promiscuous Antimicrobial Peptides. David Craik, The University of Queensland, Australia Discovery and Applications of Cyclic Peptides in Drug Development Anselmo J. Otero-Gonzalez, Havana University, Cuba Host Defense Peptides Isolated from the Litorinic Snail Cenchritis muricatus Symposium 2 Dengue Virus Proteins in Infection and Pathogenesis Chair: Andrea T. Da Poian, Univ Federal do Rio de Janeiro Miguel A.R.B. Castanho, Univ de Lisboa, Portugal Dengue Virus Capsid Protein Translocates Nucleic Acids across Lipid Membranes: Potential Role in Viral Entry Expo IV B Claudia D. dos Santos, Instituto de Biologia Molecular do Paraná Synergistic Interactions between the NS3hel and E Proteins Contribute toward the Virulence of Dengue Virus Type 1 Ada M.B. Alves, Instituto Osvaldo Cruz The NS1 and NS3 Dengue Virus Proteins: Role in Protection and/or Pathogenesis 8

10 Sunday - May 19 th, :30-10:30 am Symposium 3 Undergraduate Studies in Biochemistry in Brazil and the World: State-of-the-art, Perspectives and Job Opportunities Chair: Paulo A. Granjeiro, Univ de São João Del Rey Paulo S.L. Beirão, Univ Federal de Minas Gerais, Chair of IUBMB Education Committee Expo IV D Juliana L.R. Fietto, Univ Federal de Viçosa Update and Perspectives of the Bachelor s Degree in Biochemistry from the Universidade Federal de Viçosa (UFV) Adelar Bracht, Univ Estadual de Maringá Crucial Differences between Undergraduate Biochemical Education in Brazil and Developed Countries: History and Perspectives Symposium 4 Computational Biochemistry and Biophysics Chair: Antonio F.P. Araújo, Univ de Brasília Iguaçu I Thereza A. Soares, Univ Federal de Pernambuco What Can We Learn from Computer Simulations of the Bacterial Outer Membrane? Werner L. Treptow, Univ de Brasília Atomistic Simulations of Ion Channels in Biological Membranes Guilherme M. Arantes, Univ de São Paulo Mecanism of Bond Dissociation in Metalloproteins under Stress Sunday - May 19 th, :00 am to 12:00 pm Expo IV A Conference 2 Chair: Marilene Demasi, Instituto Butantan Efstathios S. Gonos, National Hellenic Research Foundation, Greece Proteasome Activation as a Novel Anti-Aging Strategy Expo IV B Conference 3 Chair: Mario A.C. da Silva-Neto, Univ Federal do Rio de Janeiro David G. Hardie, University of Dundee, UK AMPK: Standing at the Crossroads of Metabolism, Immunity and Neurobiology 9

11 Sunday - May 19 th, :00 am to 12:00 pm Expo IV C Conference 5 Chair: Antonio F.P. Araújo, Univ de Brasília Eugene I. Shakhnovich, Harvard University, USA Mapping Protein Folding on Organismal Fitness One Mutation at a Time Iguaçu I Conference 6 Chair: Rodrigo Serrato, Univ Federal do Paraná Russell W. Carlson, University of Georgia, USA Lipopolysaccharides in Rhizobium-Legume Symbioses Sunday - May 19 th, :00 to 4:00 pm Symposium 5 Structural Characterization and Biological Activity of Glicoconjugates Chair: Marcello Iacomini, Univ Federal do Paraná Helio K. Takahashi, Univ Federal de São Paulo New Insights in Sphingolipids and Glycosphingolipids of Pathogenic Microorganisms. Biochemical and Structural Studies Expo IV A Giangiacomo Torri, Instituto di Ricerche Chimiche e Biochimiche Giuliana Ronzoni, Italy Structural Characterization of Heparins Marco Guerrini, Instituto di Ricerche Chimiche e Biochimiche Giuliana Ronzoni, Italy Structural Variants of Antithrombin Binding Heparin Oligosaccharides: Their Influence in Heparin Biological Properties. Guilherme L. Sassaki, Univ Federal do Paraná New Insights for Glycan Analysis by Nuclear Magnetic Resonance Symposium 6 The Ubiquitin-Proteasome System Chair: Marilene Demasi, Instituto Butantan Expo IV B Marilene Demasi, Instituto Butantan Proteasomal Activation Increases the Removal of Oxidized Proteins and Prevents Protein Aggregation Renata Guerra de Sá, Univ Federal de Ouro Preto The Ubiquitin/Proteasome Systems in Schistosoma mansoni: Molecular Complexity Driving Parasite Development and Differentiation Marcelo D. Gomes, Univ de São Paulo Identification of E3 Ubiquitin-Ligase SCF1 (FBXO25) Substrates Using Protein Microarrays (Protoarray) 10

12 Sunday - May 19 th, :00 to 4:00 pm Symposium 8 SBBq-Conesul Symposium Young Scientist Platform Session Chair: José Roberto Meyer Fernandes, Univ Federal do Rio de Janeiro Alexandre U. Amaral, Univ Federal do Rio Grande do Sul Reduction of Na +, K + -ATPase Activity and Expression in Brain of Glutaryl-CoA Dehydrogenase Deficient Mice: A Possible Mechanism for Brain Injury in Glutaric Aciduria Type I Iguaçu I Bruno Chausse, Univ de São Paulo Intermittent Fasting Modulates Hypothalamic Control of Feeding and Body Mass Luis A. Luévano-Martínez, Univ de São Paulo Deletion of the Transcriptional Regulator Opi1p Affects Mitochondrial Metabolism Renan M. Monteiro, Univ Estadual do Norte Fluminense New Insights on the Regulation of the Yeast V e P H + -ATPases 4:30-6:30 pm Poster Session - Expocenter I, II and III 6:45 pm Council Meeting Paraná Room Monday - May 20 th, 2013 Symposium 9 17 o. Prize for Young Scientists in Life Sciences Chair: Carlos Termignoni, Vice-President of SBBq and Univ Federal do Rio Grande do Sul Ana Cristina Puhl, Univ de São Paulo Natural Agonists of PPARγ to be Used in the Treatment of Diabetes Expo IV A Jordano de Brito Moreira da Silva, Univ Federal do Rio de Janeiro α-neurexins and Neuroligin 1 Mediate Aβ Oligomers Bind to Hippocampal Neurons, Synaptotoxicity and Memory Impairment Laércio Pol Fachin, Univ Federal do Rio Grande do Sul Structural Glycobiology of the Heparin Role on Coagulation Cascade Mariana H.S. Massaoka, Univ Federal de São Paulo A Novel Cell-Penetrating Peptide Derived From Wilms Tumor Protein 1 (WT1) Induces Cellular Senescence and Displays Antimelanoma Activity In A Preclinical Model 11

13 Monday - May 20 th, :30 to 10:30 am Symposium 10 Energy Metabolism in Cancer Cells Chair: Franklin D. Rumjanek, Univ Federal do Rio de Janeiro Rafael Moreno-Sanchez, Instituto Nacional de Cancerologia, México Energy Metabolism in Tumor Cells Expo IV B Rodrigue Rossignol, Université Bordeaux Segalen, France Mitoplasticity: Adaptation Biology of the Mitochondrion to the Cellular Redox State in Physiology and Carcinogenesis Antonio Galina Filho, Univ Federal do Rio de Janeiro Effects of Antitumor Alkylating Agent 3-Bromopyruvate on Energy Producing and Consuming Targets in Hepatoma Cell Line HepG2, Liver Mitochondria and SERCA: Is There Any Role for Mitochondrial Hexokinase Activity? Symposium 11 Cellulose, Plant Walls and Cellulolytic Enzymes Chair: Marcos S. Buckeridge, Univ de São Paulo Fabio Squina, Lab Nacional de Ciência e Biotecnol do Bioetanol Metaproteogenomic Strategies for Enzyme Discovery in Biomass-to-Bioproducts Applications Expo IV D Carlos Driemeier, Lab Nacional de Ciência e Biotecnol do Bioetanol Ultrastructure of Cellulosics: General Considerations, Specificities of Sugarcane, and Impacts on Enzymatic Degradation Marcos S. Buckeridge, Univ de São Paulo, INCT-Bioetanol Understanding the Complexity of Cell Wall Structure to Improve Bioenergy Production Symposium 12 SBBq-Conesul Symposium Young Scientist Platform Session Chair: Fernando Luis Barroso da Silva, Univ de São Paulo Ariane Nunes-Alves, Univ de São Paulo The Role of Protein Flexibility in the Binding of Small Molecules Iguaçu I Maurício Costa, Univ Federal do Rio de Janeiro A New Approach for Efficient Sampling of Conformational Landscapes: Molecular Dynamics in Excited Normal Modes Space Cecilia Ortíz, Institut Pasteur de Montevideo, Uruguay Towards the Validation of Glucose-6-Phosphate Dehydrogenase from Trypanosoma cruzi as a Drug Target Candidate Monday - May 20 th, :00 am to 12:00 pm Expo IV A Conference 7 Chair: Richard C. Garratt, Univ de São Paulo William L. Duax, Hauptman-Woodward Institute, USA Evolutionary Tree Based on Ribosomal Proteins 12

14 Monday - May 20 th, :00 am to 12:00 pm Expo IV B Expo IV D Iguaçu I Expo IV C Conference 8 Chair: Anibal E. Vercesi, Univ Estadual de Campinas Roberto Docampo, University of Georgia, USA The Role of Polyphosphates in the Biology of Trypanosomes Conference 9 Chair: Emanuel M.Souza, Univ Federal do Paraná Philip S. Poole, John Innes Centre, UK The Plant Microbiome, Association between Bacteria and Plants Conference 10 Chair: Maria Elena De Lima, Univ de Minas Gerais Cesare Montecucco, Universita'di Padova, Italy On the Mechanism of Action of Snake PLA2-Neurotoxins and -Myotoxins Conference 11 Chair: Hugo Verli, Univ Federal do Rio Grande do Sul José Manuel Estevez, Facultad de Ciencias Exactas y Naturales, Argentina Control of Single Cell Polarized Growth by O-Glycoproteins Monday - May 20 st, :00 to 4:00 pm Expo IV C Discussion Group Bioenergetics, Metabolism and Membranes (Portuguese) Coordinator: Alicia J. Kowaltowski, Univ de São Paulo Monday - May 20 th, :00 to 4:00 pm Expo IV A Symposium 13 New Insights in the Function of Molecular Chaperones Chair: Carlos H.I. Ramos, Univ Estadual de Campinas Walid A. Houry, University of Toronto, Canada The Role of Hsp90-R2TP Complex in snornp Assembly and Ribosomal RNA Processing Cristina Bonorino, Pontifícia Univ Católica do Rio Grande do Sul The Anti-Inflammatory Mechanisms of HsP70 Carlos H. I. Ramos, Univ Estadual de Campinas The Identification of a Human Protein Potentially Involved with Thermal Stress and Protein Disaggregation Expo IV B Symposium 14 From Neurotransmission to Neuroprotection: A Neurochemical Promenade Chair: Carmem Gottfried, Univ Federal do Rio Grande do Sul Peter R. Dunkley, The University of Newcastle, Faculty of Health The Timing of Tyrosine Hydroxylase Phosphorylation is Everything Ayse Dosemeci, National Institutes of Health, USA CaMKII Mediates Molecular Re-Organization at the Postsynaptic Density Carlos A.S. Gonçalves, Univ Federal do Rio Grande do Sul Rod s Journey in Neurochemistry: from England to Brazil 13

15 Monday - May 20 th, :00 to 4:00 pm Expo IV D Symposium 15 Plant-Pathogen Interactions: Molecular Events and Signaling Chair: Ivan de Godoy Maia, Univ Estadual Paulista Sylvie German-Retana, Equipe de Virologie, UMR, France Functional Analysis of Plant-Potyvirus Interactions Aline M. Da Silva, Univ de São Paulo Xylella fastidiosa Comparative Genome Analyses Provide Insights into Pathogen-Plant Interaction Celso E. Benedetti, Laboratório Nacional Biociências, CNPEM A MAP Kinase Signaling Cascade Involved in Citrus Canker Resistance Symposium 16 SBBq-Conesul Symposium Young Scientist Platform Session Chair: Sayuri Miyamoto, Univ de São Paulo Carolina G. Fernandes, Univ Federal do Rio Grande do Sul Neurochemical Evidence that the Major Metabolites Accumulating in 3-Hydroxy-3Mmethylglutaric Aciduria Induce Oxidative Stress In Vivo in Striatums of Young Rats Iguaçu I Carolina Prolo, Univ de La Republica, Uruguay Superoxide Radical-Dependent Mechanisms in the Control of Trypanosoma cruzi Infection in Macrophages Monday - May 20 st, :30 to 6:30 pm 4:30 to 6:30 pm Maria Fernanda Forni, Univ de São Paulo Glutathione as a Key Regulator of Skin Stem Cell Homeostasis: From Embryonic Development to Ageing Florencia Sardi, Institut Pasteur de Montevideo, Uruguay Monitoring Intracellular Redox Changes in Trypanosomatids and Host Cells using Redox-Sensitive Fluorescent Proteins Poster Session - Expocenter I, II and III Autograph Session - Expocenter III Técnicas em Biociências Protocolos Comentados para o seu Laboratório. Autoria de MONICA MONTERO LOMELI e FRANKLIN RUMJANEK 6:45 pm General Assembly Expocenter C Tuesday - May 21 st, :30 to 12:30 pm Expo IV C Discussion Group Glicobiologia (Portuguese) Coordinator: Hugo Verli, Univ Federal do Rio Grande do Sul Tuesday - May 21 st, :30 to 10:30 pm Expo IV A Symposium 17 Protein Aggregation in Function and Disease Chair: Yraima M.L.Cordeiro, Univ Federal do Rio de Janeiro Salvador Z. Ventura, Universidad Autonoma de Barcelona, Spain An Integrated Experimental and Computational Approach to Study Protein Aggregation Richard C. Garratt, Univ de São Paulo How to Assemble a Septin Filament Yraima M.L. Cordeiro, Univ Federal do Rio de Janeiro Interaction of the Prion Protein with Ligands: Participation in the Pathogenesis of Spongiform Encephalopaties 14

16 Tuesday - May 21 st, :30 to 10:30 pm Symposium 18 Brazil-Chile Symposium (SBBq-SBM ) Perspectives in Cancer Research Chair: Ricardo J. Giordano, Univ de São Paulo Andrew Quest, Universidad de Chile Synergism Versus Antagonism Between Caveolin-1 and E-Cadherin in Tumor Suppression and Metastasis Expo IV B Roxana Pincheira, Universidad de Concepción, Chile The Sall2 Transcription Factor and Cancer Ricardo J. Giordano, Univ de São Paulo Exploring with Combinatorial Approaches the Molecular Diversity of Blood Vessels in Health and Disease Franklin D. Rumjanek, Univ Federal do Rio de Janeiro Dissecting the Role of Mitochondria in Cancer Cells Symposium 19 Arthropod Molecular Physiology and Immunology: Advances and Perspectives Chair: Fernando A. Genta, FIOCRUZ-IOC Marcos H.F. Sorgine, Univ Federal do Rio de Janeiro Bodies of Evidence: Lipid Bodies and Eicosanoids in Mosquito Immunity Expo IV D Sirlei Daffre, Univ de São Paulo The Tick Response to Rickesttsial Infection Rafael D. Mesquita, IFRJ Using Transcriptome to Train Gene Prediction Software in Rhodnius prolixus Adriana Seixas, Univ Federal do Rio Grande do Sul Proteins Involved in Tick Embryogenesis: Targets for an Anti-Tick Vaccine Symposium 20 In Silico Methods Applied to Explore Structural and Biological Features Chair: Ana M. Chudzinski-Tavassi, Instituto Butantan Kaline R. Coutinho, Univ de São Paulo Theoretical Study of Molecules in Solution and Phospholipid Bilayers Iguaçu 1 Kerly F.M. Pasqualoto, Instituto Butantan In Silico Analysis to Explore a Peptide Derivative as Lipocalins' Signature Marcia M.C. Ferreira, Univ Estadual de Campinas Chemometric Methods in Biomedical Research Roberto D. Lins Neto, Univ Federal de Pernambuco Predictive Biomolecular Modeling in Proteomics 15

17 Tuesday May 21 st :00 am to 12:00 pm Expo IV A Expo IV B Expo IV D Conference 12 Chair: Fabio de O. Pedrosa, Univ Federal do Paraná Stefan Nordlund, Stockholm University The Central Role of PII Proteins in the Regulation of Nitrogen Metabolism in Prokaryotes - Especially Nitrogenase Activity in Rhodospirillum rubrum Conference 13 PABMB Lecture Chair: Alicia J. Kowaltowski, Univ de São Paulo Sergio Lavandero, Universidad de Chile Intraorganelle Communication in Normal and Diseased Cells Conference 14 Chair: Marcos H.F. Sorgine, Univ Federal do Rio de Janeiro David S. Schneider, Stanford University, USA Warping Disease Space to Improve Recovery from Infections Iguaçu I Conference 15 Chair: Moacir Wajner, Univ Federal do Rio Grande do Sul Phillip W. Disckon, University of New Castle, Australia Tyrosine Hydroxylase and Alpha-Synuclein in Parkinson's Disease Tuesday - May 21 st, :00 to 4:00 pm Symposium 21 SBBq-SBBM, Uruguay Symposium: Oxidative Processes: from proteins to disease Chair: Andrés Trostchansky, President of the Sociedade de Bioquímica e Biologia Molecular do Uruguay Lucia Turell, Univ de La Republica, Uruguay Oxidative Derivatives of the Albumin Thiol with Focus on Sulfenic Acid Expo IV A Adriana Cassina, Univ de La Republica, Uruguay Mitochondrial Dysfunction in Amyotrophic Lateral Sclerosis: Prevention by Mitochondrial Antioxidants Flavia C. Meotti, Univ de São Paulo Oxidation of Uric Acid by Myeloperoxidase in Inflammatory Processes and Implications for Cardiovascular Disease Jose Carlos Toledo Junior, Univ de São Paulo Kinetics and Mechanisms of Nitrosation in Cells Symposium 22 Cellular Signaling in Parasites Chair: Célia R.S. Garcia, Univ de São Paulo Silvia Moreno, University of Geórgia, USA Calcium Homeostasis in the Apicomplexan Parasite T. gondii Expo IV B Anibal E. Vercesi, Univ Estadual de Campinas Ca 2+ Transport by the T. cruzi Mitochondrion: Role in Apoptosis and Necrosis-Like Processes Célia R.S. Garcia, Univ de São Paulo GPCR Signaling in P. falciparum and the Search for its Molecular Partners Kildare R. Miranda, Univ Federal do Rio de Janeiro Signaling Pathways Involved in the Dynamic Control of the Contractile Vacuole Complex in Trypanosomatid Parasites 16

18 Tuesday - May 21 st, :00 to 4:00 pm Symposium 23 SBBq-Conesul Symposium Young Scientist Platform Session Chair: Russolina Zingali, Univ Federal do Rio de Janeiro Letícia F. Terra, Univ de São Paulo Molecular Mechanisms Involved in Cytoprotection and Malignant Transformation of Human β-cells Expo IV D Amy Mónaco, Instituto de Higiene, Uruguay Development of New Immunotherapies against Melanoma Based on the Use of Salmonella Carrying Plasmids Encoding for IL18 Laura de A. Moura, Univ Federal Fluminense Antiplatelet and Anticoagulant Effects of Two Diterpenes Isolated from the Brazilian Marine Algae D. menstrualis Mardja M.B. e Silva, Univ Federal do Rio Grande do Sul Resveratrol Enhances the Chronic Citotoxic Effect of Temozolomide in Glioma Cells: Role of Senescence and DNA Damage Symposium 24 SBBq-Conesul Symposium Young Scientist Platform Session Chair: Emanuel M. de Souza, Univ Federal do Paraná Jackelyn E. Borja, Universidad Nacional Mayor de San Marcos, Peru Halotolerant Bacteria Producing Extracellular Hydrolytic Enzymes Isolated from Tarapoto Hot Springs in Peru Iguaçu I Angélica M. Gomes, Univ Federal do Rio de Janeiro Heparanase Modulates Mammary Branching Morphogenesis by Regulating MMP-14 Level Gabriela Ayrosa, Instituto Butantan Juruin: An Antifungal Juruentoxin Peptide from the Venom of the Amazonian Pink Toe Spider, Avicularia juruensis, Which Contains the Inhibitory Cystine Knot Motif Geisa Evaristo, Univ Estadual do Norte Fluminense Mass Spectrometry Approaches to Discover and De Novo Sequence Peptides Carrying Post- Translation Modifications Tuesday - May 21 st, :30 to 6:30 pm - Expocenter II and III Poster Session 6:45 to 8:00 pm - Expocenter IV A and IV B Closing Ceremony and SBBq Awards Ceremony Announcement of SBBq Awards for the best poster presentations and the XVII Young Talent in Life Sciences Prize 17

19 Discussion Group: The thematic discussion fosters the interaction among groups with common research interest complementing the poster session and symposia 1. GLICOBIOLOGY Chair: Hugo Verli, UFRGS Tuesday, May 21, 2013 Local: Expocenter IV C 8:30 to 12 am 2. Energetics Metabolism Chair: Alicia Kowaltowski, USP-IQ Monday, May 20, 2013 Local: Expocenter IV C 14 to 16 am 18

20 Technical Seminary Sunday May 19 th, 2013 Iguaçu V (5 th floor) - Sinapse 08:30-10:00 Soluções para expressão gênica do RNA ao qpcr. Carlos André de Castro Herklotz, MBA 10h00-11h30 DNA-Exitus, RNase-Exitus, Autoclave-Exitus and Derma-Exitus including case studies for DNA-Exitus. Sussane Frasch, MBA Iguaçu IV (5 th floor) - Roche 14:00-18:00 Evento de Lançamento Roche Paraná III - BD 09:00-12:00 Simplificando a Citometria de Fluxo do princípio aos resultados e Traga suas dúvidas relacionadas à citometria de fluxo para discussão 14:00-16:00 exclusiva com o palestrante. É a sua chance de discutir e aprender sobre a técnica, aspectos críticos, aplicações e soluções para citometria de fluxo e cell sorting. Enio Bassi, MSc, PhD 12:00-13:00 BD Accuri : Citometria de Fluxo ao alcance de todos. Soluções em citometria de fluxo intracelular, BD CBA, ciclo e viabilidade celular, proliferação, apoptose, estudo de vias de sinalização celular proteínas. Enio Bassi 16:00-17:00 Aplicações da metodologia de Cell Sorting em biologia molecular Novo BD FACSJazz : isolamento celular - cell sorting - de bancada com preço acessível. Enio Bassi Paraná IV: AB SCIEX Mass Spec Seminar 09:00-10:00 Global Quantitative Proteomics with MS/MS ALL using SWATH Acquisition Helio Martins Júnior, MSc 10:00-11:00 High Performance Tissue Imaging on the AB SCIEX TOF/TOF 5800 System Helio Martins Júnior 11:00-12:00 Quantitative Shotgun Lipidomics using MS/MS ALL workflow on the TripleTOF technology. Helio Martins Júnior Paraná IV: Molecular Devices 14:00-15:30 New SpectraMax Platform: Now you can image Cells with our new microplate reader! Heitor Franco 15:30-17:00 Creating new tools for cellular imaging analysis with Custom Module Editor Luciane Ganiko Parará VI: Illumina 08:30-10:00 Sample Prep Evolution. Bye, Bye PCR! Introducing a new PCR-free Sample Prep Solution. New Generation of DNA Targeted Enrichment Products. Say Hello to Moleculo! Enabling synthetic long reads of up to 10kb Guilherme Mendes, Ana Paula Aidar, Veridiana Cano 10:30-12:00 Solutions for RNA from Discovery to Validation. NGS can now replace Real- Time PCR (qpcr) for mid-plex gene expression and validation studies. New TruSeq Targeted RNA kits!. Guilherme Mendes, Ana Paula Aidar and Veridiana Cano 19

21 Parará VI: Uniscience 14h00-14h50 Revelando o Westen Blotting: Uma análise detalhada do protocolo. Bruno Oliveira, MSc 15h00-15h50 Digital, multiplexed measurements of up to 800 mrna targets in clinically relevant or plant sample types using the NanoString ncounter Analysis System Jacques Corriveau, MBA 16h00-16h50 PacBio Single Molecule Real-Time (SMRT ) Sequencing for De Novo Sequencing and Assembly of Complex Organisms. Ram Laxman, Ph.D. 17h00-17h50 Preparação de amostras para Sequenciamento de Nova Geração, as soluções da Covaris. Bruno Oliveira, Msc. Paraná VII: Leica Microsystems 09:00-10:00 Principles of Confocal Microscopy: Leica TCS SP8. Marcio Mesquita 10:30-12:00 Measuring Calcium Fluorescence in different cellular models Mr. Piero Bagnaresi, PhD Paraná VII: Beckman Coulter 14:00-15:30 The Importance of Choosing the Ideal Fluorochrome for Flow Cytometry Helena Barros Monday May 20 th, 2013 Iguaçu IV (5 th floor): Qiagen 14:00-18:00 Speed up and simplify your workflow: Pre analytic QIAGEN solutions for NGS Iguaçu V (5 th floor): Sinapse 09:00-11:00 Western Blotting Workflow Presentation. Priya Rangaraj, PhD 11:00-12h00 myecl Imager One-touch image capture and analysis for protein and nucleic acid gels and blots. Navid Haghdoost, PhD Iguaçu V (5 th floor): Eppendorf 14:00-18:00 UV-VIS Spectroscopy: Still an Important Tool for common Biochemistry and Molecular Biology Applications. Martin Armbrecht, Ph.D Paraná III: Waters: Proteômica, Metabolômica e Lipidômica 08:30-09:20 Conceitos e Estratégias de Estudos de Metaboloma e Lipidoma. Michael Murgu 09:30-10:20 Pesquisador convidado 10:30-11:20h nanodecodificação de Proteínas: Como Decifrar a Constituição Celular por Cromatografia e Espectrometria de Massas. Gustavo H. M. F. Souza Paraná III: Sigma-Aldrich 14:00 Silenciamento de Genes: Bibliotecas de shrnas e sirnas 15:00 A Rotina do PCR e qpcr: Novas Fronteiras 16:00 Proteômica: Das Proteínas Recombinantes à Depleção 20

22 Paraná IV: GE Healthcare Life Sciences - Mini-Curso 09h30-10h30 Getting it Right from the Start do cultivo celular ao preparo da amostra. Ana Paula Gregio, PhD 11h00-12h00 Como desenhar um procedimento para purificação de proteínas. Estratégias de utilização das ferramentas cromatográficas para obtenção de proteínas com alto grau de pureza. Alécio Pimenta Jr., PhD 14h30-15h30 Interactoma o estudo de interações moleculares em tempo real. Maurício Marques, PhD 15h45-16h45 Análise de multiparâmetros celulares (HCA) por microscopia de fluorescência Sandra Rosa, Msc 17h00-18h00 Como desenhar um procedimento para purificação de proteínas. Estratégias de utilização das ferramentas cromatográficas para obtenção de proteínas com alto grau de pureza. Alécio Pimenta Jr., PhD Paraná VI: Life Technologies 08h00-08h50 Carreiras no mundo de Life Sciences. Fernanda Cagno 09h00-09h50 O uso da Biologia Sintética na Pesquisa nacional. Pesquisador Convidado 10h00-10h50 Do isolamento à análise funcional: o vasto mundo dos micrornas Ana Maria Fraga, PhD. 11h00-11h50 Western-blotting: nunca foi tão fácil e rápido obter as melhores bandas! Tatiana Ortolan, PhD 14h00-14h50 Ion Torrent: Como sequenciar um genoma por 1000 dólares. Raphael Fonseca, M.Sc 15h00-15h50 Attune Acoustic Focusing Cytometer: Precision and Sensitivity at All Speeds Claudia Pantaleão, PhD 16h00-16h50 Como otimizar a performance de seus equipamentos. Augusto Carminato 17h00-18h00 Carreiras no mundo de Life Sciences. Fernanda Cagno Paraná VII Agilent Technologies 09:00-10:00 Metabolômica: de pequenas moléculas a grandes soluções! Uso da Espectrometria de Massas de Alta Resolução. Alana Figueiredo 10:00-11:00 Integrated Biology: A Pathway-centric approach to Multi-omics research Dra. Carolina Livi 14:00-15:00 O seu laboratório está preparado para um número cada vez maior de variedades de aplicações bioanalíticos para HPLC? Simone Nascimento 15:00-16:00 Desvendando a função de RNAs não codificadores: uma análise integrada Yuri Moreira 16:00-17:00 Soluções genômicas em pesquisa clínica e câncer. Douglas Blake 21