biomapping Genomics Biostock Sigma-Aldrich 2011 Tudo Para a Sua Rotina da Molecular!

Transcrição

1 biomapping Genomics Biostock Sigma-Aldrich 2011 Tudo Para a Sua Rotina da Molecular!

2 2 biomapping PCR Quantitativo (DNA e RNA) Biostock Sigma-Aldrich D RXN JumpStart Taq ReadyMix for Quantitative PCR r D RXN JumpStart Taq ReadyMix for Quantitative PCR r 1, L RXN LuminoCt qpcr ReadyMix with Reference Dye r (Fast qpcr) L RXN LuminoCt qpcr ReadyMix with Reference Dye r (Fast qpcr) 2, QR0200 Quantitative RT-PCR ReadyMix r D RXN JumpStart Taq ReadyMix for High Throughput Quantitative PCR - 20 r D RXN JumpStart Taq ReadyMix for High Throughput Quantitative PCR r 1, S RXN SYBR Green JumpStart Taq ReadyMix for quantitative PCR r S RXN SYBR Green JumpStart Taq ReadyMix for quantitative PCR r 1, L RXN LuminoCt SYBR Green qpcr ReadyMix with Reference Dye r (Fast qpcr) L RXN LuminoCt SYBR Green qpcr ReadyMix with Reference Dye r (Fast qpcr) 2, QR0100 SYBR Green Quantitative RT-PCR Kit r S RXN SYBR Green JumpStart Taq ReadyMix for High Throughput qpcr - 20 r S RXN SYBR Green JumpStart Taq ReadyMix for High Throughput qpcr r 1, PCR e RT-PCR (enzimas e mixings) D UN Taq DNA Polymerase (with 10 buffer containing MgCl), rec u (5 u/µl) D KU Taq DNA Polymerase (with 10 buffer containing MgCl), rec u (5 u/µl) D UN Taq DNA Polymerase (with 10 buffer without MgCl), rec u (5 u/µl) D KU Taq DNA Polymerase (with 10 buffer without MgCl), rec u (5 u/µl) D UN AccuTaq LA DNA Polymerase (Long PCR - HiFi) u (5 u/µl) D UN AccuTaq LA DNA Polymerase (Long PCR - HiFi) u (5 u/µl) D UN JumpStart Taq DNA Polymerase u (2,5 u/µl) D UN JumpStart Taq DNA Polymerase without MgCl u (2,5 u/µl) D UN JumpStart AccuTaq LA DNA Polymerase u (2,5 u/µl) D UN REDTaq DNA Polymerase u (1 u/µl) D UN JumpStart REDTaq DNA Polymerase u (1 u/µl) D UN JumpStart REDAccuTaq LA DNA Polymerase u (1 u/µl) P RXN ReadyMix Taq PCR Reaction Mix with MgCl (50 µl volume final) P RXN JumpStart Taq ReadyMix (50 µl volume final) R RXN REDTaq ReadyMix PCR Reaction Mix (50 µl volume final) P RXN JumpStart REDTaq ReadyMix PCR Reaction Mix (50 µl volume final) M KU M-MLV Reverse Transcriptase u (200 u/µl) A4464-1KU Enhanced Avian Reverse Transcriptase - eamv (20 u/µl; 50 reações) 1, STR1-1KT Enhanced Avian First Strand Synthesis Kit (20 µl volume final; 50 reações) 1, HSRT100-1KT Enhanced Avian HS RT-PCR Kit reações 2, O ML Oligo(dT)23, Anchored 70 μm in H 2 O (0,5 ug/µl; 100 µl) R UL Random Nonamers 50 μm in H 2 O (100 µl; 100 r) R KU Ribonuclease inhibitor human ( u/ml) u R KU Ribonuclease inhibitor human ( u/ml) u DNTP10-1KT Deoxynucleotide Set (datp, dctp, dgtp, dttp), 10 mm µl cada DNTP100-1KT Deoxynucleotide Set (datp, dctp, dgtp, dttp), 100 mm µl cada

3 3 D ML Deoxynucleotide Mix, 10 mm - PCR Reagent ul (1 ul/pcr 50 ul vol final) D ML Deoxynucleotide Mix, 10 mm - PCR Reagent ul (1 ul/pcr 50 ul vol final) A TST JumpStart Taq Antibody - 80 ul B0300-1VL Betaine solution 5 M, PCR Reagent - 1,5 ml D9170-1VL Dimethyl sulfoxide, PCR Reagent (1-10 %) - 1 ml G8778-1VL Glycerol, PCR Reagent (5-10%) - 1,5 ml Extração e amplificação XNAB2-1KT Extract-N-Amp Blood PCR Kit extrações / 100 amplificações XNAP2-1KT Extract-N-Amp Plant PCR Kit extrações / 100 amplificações XNAT2-1KT Extract-N-Amp Tissue PCR Kit extrações / 100 amplificações E ML Extract-N-Amp PCR ReadyMix amplificações XNAB-1KT REDExtract-N-Amp Blood PCR Kit extrações / 100 amplificações XNAP-1KT REDExtract-N-Amp Plant PCR Kit extrações / 100 amplificações XNAT-1KT REDExtract-N-Amp Tissue PCR Kit extrações / 100 amplificações R ML REDExtract-N-Amp PCR ReadyMix amplificações XNATG-1KT SYBR Green Extract-N-Amp Tissue PCR Kit extrações / 100 amplificações XNAPG-1KT SYBR Green Extract-N-Amp Plant PCR Kit extrações / 100 amplificações S ML SYBR Green Extract-N-Amp PCR ReadyMix amplificações Amplificação Genômica WGA2-10RXN GenomePlex Complete Whole Genome Amplification (WGA) Kit - 10 reações WGA4-10RXN GenomePlex Single Cell Whole Genome Amplification Kit - 10 reações WTA2-10RXN TransPlex Complete Whole Transcriptome Amplification Kit - 10 reações 1, Purificação de RNA (total, mrna e mirna) R ML RNAlater T ML TRI Reagent - 25 ml T ML TRI Reagent ml T ML TRI Reagent BD - 25 ml T ML TRI Reagent BD ml T ML TRI Reagent LS - 25 ml T ML TRI Reagent LS ml RTN10-1KT GenElute Mammalian Total RNA Miniprep Kit - 10 preps RTN70-1KT GenElute Mammalian Total RNA Miniprep Kit - 70 preps 1, MRN10-1KT GenElute mrna Miniprep Kit - 10 preps MRN70-1KT GenElute mrna Miniprep Kit - 70 preps 2, DMN10-1KT GenElute Direct mrna Miniprep Kit - 10 preps DMN70-1KT GenElute Direct mrna Miniprep Kit - 70 preps 2, SNC10-1KT mirpremier microrna Isolation Kit - 10 preps SNC50-1KT mirpremier microrna Isolation Kit - 50 preps STRN10-1KT Spectrum Plant Total RNA Kit - 10 preps STRN50-1KT Spectrum Plant Total RNA Kit - 50 preps R ML RNaseZAP AMPD1-1KT DNase I Amplification Grade u (1 u/ul) D5758-5ML Diethyl-pyrocarbonate (DEPC) D ML Diethyl-pyrocarbonate (DEPC) R8508-1ML trna from baker's yeast (S. cerevisiae), 10 mg/ml - 1 ml

4 4 biomapping DNA Plasmidial PFM50-1KT Biostock GenElute Sigma-Aldrich Five-Minute Plasmid Miniprep Kit - 50 preps PFM250-1KT GenElute Five-Minute Plasmid Miniprep Kit preps 1, NA0150-1KT GenElute HP Plasmid Miniprep Kit - 70 preps NA0160-1KT GenElute HP Plasmid Miniprep Kit preps 1, NA0200S-1KT GenElute HP Plasmid Midiprep Kit - 4 preps NA0200-1KT GenElute HP Plasmid Midiprep Kit - 25 preps NA0300S-1KT GenElute HP Plasmid Maxiprep Kit - 4 preps NA0300-1KT GenElute HP Plasmid Maxiprep Kit - 10 preps NA0310-1KT GenElute HP Plasmid Maxiprep Kit - 25 preps 1, NA0500-1KT GenElute HP Plasmid Megaprep Kit - 5 preps 1, PLED35-1KT GenElute Endotoxin-free Plasmid Midiprep Kit - 35 preps 1, NA0400S-1KT GenElute HP Endotoxin-Free Plasmid Maxiprep Kit - 4 preps NA0400-1KT GenElute HP Endotoxin-Free Plasmid Maxiprep Kit - 10 preps NA0410-1KT GenElute HP Endotoxin-Free Plasmid Maxiprep Kit - 25 preps 1, NA0600-1KT GenElute HP Endotoxin-Free Plasmid Megaprep Kit - 5 preps 1, NA0100-1KT PhasePrep BAC DNA Kit 1, DNA Genômico e Fragmentos NA2010-1KT GenElute Blood Genomic DNA Kit - 70 preps NA2020-1KT GenElute Blood Genomic DNA Kit preps 2, G1N70-1KT GenElute Mammalian Genomic DNA Miniprep Kit - 70 preps G1N350-1KT GenElute Mammalian Genomic DNA Miniprep Kit preps 2, G2N70-1KT GenElute Plant Genomic DNA Miniprep Kit - 70 preps G2N350-1KT GenElute Plant Genomic DNA Miniprep Kit preps 2, NA2110-1KT GenElute Bacterial Genomic DNA Kit - 70 preps NA2120-1KT GenElute Bacterial Genomic DNA Kit preps 2, NA1111-1KT GenElute Gel Extraction Kit - 70 preps NA1020-1KT GenElute PCR Clean-Up Kit - 70 preps P G Phenol for molecular biology g P G Phenol for molecular biology g P ML Phenol solution Equilibrated with 10 mm Tris HCl, ph 8.0, 1 mm EDTA ml P ML Phenol solution Equilibrated with 10 mm Tris HCl, ph 8.0, 1 mm EDTA ml P ML Phenol solution Saturated with 0.1 M citrate buffer, ph 4.3 ± ml P ML Phenol solution Saturated with 0.1 M citrate buffer, ph 4.3 ± ml P ML Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with TE ml P ML Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with TE ml P ML Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with TE (w/buffer) ml P ML Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with TE (w/buffer) ml ML Chloroform isoamyl alcohol mixture Fluka, 24:1, 99.5% ml C ML Chloroform contains amylenes as stabilizer, 99% - 25 ml I ML 2-Propanol for molecular biology, 99% - 25 ml I ML 2-Propanol for molecular biology, 99% ml E ML Ethanol, absolute, 100 % (200 proof ) ml

5 5 E ML Ethanol, absolute, 95 % (190 proof ) ml G G Guanidine hydrochloride, 99% - 25 g G G Guanidine hydrochloride, 99% g G G Guanidine thiocyanate, 99% g G G Guanidine thiocyanate, 99% g ML Guanidine thiocyanate solution Fluka, 4 M in H 2 O - 50 ml P MG Proteinase K, 30 units/mg protein, lyophilized - 25 mg P MG Proteinase K, 30 units/mg protein, lyophilized mg P4850-1ML Proteinase K, >800 u/ml, buffered aqueous glycerol solution - 1 ml L X1ML Lysozyme from chicken egg white, 10 mg/ml - 10 x 1 ml L KU Lyticase from Arthrobacter luteus, lyophilized powder, 2,000 u/mg protein G1767-1VL Glycogen from Mytilus edulis (Blue mussel), 20 mg/ml - 1 ml P G Polyethylene Glycol, wt g P5413-1KG Polyethylene Glycol, wt kg R MG Ribonuclease A (from Pancreas Bovine), lyophilized - 10 mg R MG Ribonuclease A (from Pancreas Bovine), lyophilized - 50 mg R MG Ribonuclease A (from Pancreas Bovine), buffered aqueous glycerol solution - 10 mg Marcadores Moleculares e Ladders de DNA P UG 20 bp Low Ladder - 80 pistas S UG Step Ladder, 50 bp pistas P1473-1VL 100 bp Ladder ul (75 pistas) D MG 123 bp Ladder pistas D0428-1VL 1 kb Ladder pistas D UG Lambda DNA Hind III Digest pistas D UG φx174 DNA Marker Hae III Digest - 80 pistas D7058-1VL DirectLoad Wide Range DNA Marker; bp; 16 bandas (100 pistas) D3812-1VL DirectLoad Step Ladder, 50 bp; bp; 17 bandas (100 pistas) D3687-1VL DirectLoad 100 bp Ladder; bp; 10 bandas (75 pistas) D3937-1VL DirectLoad 1 kb DNA Ladder; bp; 11 bandas (100 pistas) P9577-1VL PCR Marker solution; bp; 8 bandas; w/loading for samples (50 pistas) D ML Pulse Marker kb - 25 aplicações D ML Pulse Marker 50-1,000 kb - 25 aplicações D ML Pulse Marker 225-2,200 kb - 25 aplicações Marcadores Moleculares de RNA* R UG Transcript RNA Markers kb - 25 aplicações R UG Transcript RNA Markers kb - 25 aplicações R7644-1VL Transcript RNA Markers kb - 25 aplicações Marcadores Moleculares de Proteínas C1992-1VL ColorBurst Electrophoresis Marker mol wt 8, ,000 Da µl C4861-1VL ColorBurst Electrophoresis Marker High Range, mo w 30, ,000 Da µl C6210-1VL Color Marker Ultra-low Range, mol wt 1,060-26,600 Da µl C ML Color Marker Low Range, mol wt 6,500-45,000 Da µl SDS7B2-1VL Prestained Molecular Weight Marker mol wt 26, ,000 Da aplicações M3913-1VL SigmaMarker Low Range, mol wt 6,500-66,000 Da (100 µl após reconstituição) S8445-1VL SigmaMarker Wide Range, mol wt 6, ,000 Da (100 µl após rec) S8320-1VL SigmaMarker High Range, mol wt 36, ,000 Da (100 µl após rec)

6 6 biomapping M3546-1VL Ultra-low Range Molecular Weight Marker, mol wt 1,060-26,600 Da µl SDS7-1VL Biostock Molecular Sigma-Aldrich Weight Marker, mol wt 14,000-66,000 Da (1,5 ml após rec) SDS6H2-1VL Molecular Weight Marker, mol wt 30, ,000 Da (1,5 ml após rec) I3018-1VL IEF Marker mix, PI (250 µl após rec) Corantes e Intercalantes B2025-1EA Brilliant Blue G - Colloidal Concentrate (1 L após diluição) B8522-1EA Brilliant Blue G - Solution Concentrate (1 L após diluição) B8647-1EA Brilliant Blue R - Concentrate, SDS-PAGE (1 L após diluição) G ML EZBlue Gel Staining Reagent ml P7170-1L Ponceau S solution - 1 L S ML SYPRO Ruby Protein Gel Stain (2D) ml UL Nancy-520, 5,000, 0,5 ml (limite detecção > 0,5 ng DNA) E ML Ethidium bromide solution, 10 mg/ml, 10 ml ( > 1 ng DNA) E1385-5ML Ethidium bromide solution, 500 μg/ml, 5 ml ( > 1 ng DNA) E7637-1G Ethidium bromide powder, 1 g ( > 1 ng DNA) S ML SYBR Green I nucleic acid gel stain, 10,000x in DMSO, 0,5 ml (> 60 pg DNA) 1, T8935-1L BlueView Nucleic Acid Stain, powder (1 L in 10x TAE buffer); > 200 ng DNA T9060-1L BlueView Nucleic Acid Stain, powder (1 L in 10x TBE buffer); > 200 ng DNA G2526-5ML Gel Loading Buffer (w/bromophenol blue 0.05% w/v), 5 ml G7654-5ML Gel Loading Solution (w/br blue 0.25% w/v + Xylene cyanole 0.25% w/v), 6x conc Enzimas de Modificação D UN T4 DNA Ligase u D UN T4 DNA Ligase u D UN T4 DNA Polymerase - 50 u D UN T4 DNA Polymerase u P UN T4 Polynucleotide Kinase u P UN T4 Polynucleotide Kinase u R6501-1VL Ribonuclease H - 30 u P4978-1KU Phosphatase Alkaline (from calf intestine) u Enzimas de Restrição R UN Acc I u R UN Alu I u R4258-2KU Apa I u R UN Ava II u R0260-1KU BamH I u R UN Bcl I u R UN Bgl I u R UN Bgl II u R UN BstE II u R1761-1KU Cfo I u

7 7 R UN Cla I u R UN Dde I u R4381-2KU Dra I u R4640-5KU EcoR I u R5628-1KU Hae III u R1137-5KU Hind III u R UN Hpa II u R1258-1KU Kpn I u R UN MluN I - 50 u R4506-2KU Msp I u R UN Nco I u R UN Nde I u R UN Not I u R UN Pst I u R UN Pvu I - 50 u R2631-1KU Pvu II u R4756-1KU Rsa I u R5268-1KU SAC I u R0754-1KU Sal I u R UN Sau3A I u R5007-1KU Sca I u R UN Sma I u R UN Spe I u R UN Sph I u R UN Ssp I u R9507-2KU Taq I u R7260-2KU Xba I u R KU Xho I u Membranas para Blotting EA Blotting-Nylon 66 membranes, positive, roll, 0,2 m x 3 m 1, N7892-5EA Nitrocellulose membranes, pore size 0.2 μm, 15 cm 15 cm N8267-5EA Nitrocellulose membranes, pore size 0.45 μm, 15 cm 15 cm P2938-1ROL Immobilon -P PVDF membranes, roll, 0,265 m 3,75 m 1, Anticorpos Secundários Conjugados e Substratos A ML Anti-Goat IgG Alk Phosphatase, produced in rabbit ul A5420-1ML Anti-Goat IgG Peroxidase, produced in rabbit ul F ML Anti-Goat IgG FITC, produced in rabbit ul C2821-1ML Anti-Goat IgG Cy3, produced in rabbit ul T7028-1ML Anti-Goat IgG TRITC, produced in rabbit ul A ML Anti-Mouse IgG Alk Phosphatase, produced in goat ul A9044-2ML Anti-Mouse IgG Peroxidase, produced in rabbit ul F ML Anti-Mouse IgG FITC antibody produced in goat ul C2181-1ML Anti-Mouse IgG Cy3, produced in sheep ul T ML Anti-Mouse IgG TRITC, produced in goat ul A ML Anti-Rabbit IgG Alk Phosphatase, produced in goat ul A0545-1ML Anti-Rabbit IgG Peroxidase, produced in goat ul F ML Anti-Rabbit IgG FITC, produced in goat ul

8 8 biomapping T6778-1ML Anti-Rabbit IgG TRITC, produced in goat ul B ML Biostock BCIP /NBT Sigma-Aldrich Liquid Substrate System ml B ML BCIP /NBT solution, premixed ml B5655-5TAB SIGMAFAST BCIP /NBT tablet B TAB SIGMAFAST BCIP /NBT tablet F4523-5SET SIGMAFAST Fast Red TR/Naphthol AS-MX Tablet T ML 3,3,5,5 -Tetramethylbenzidine (TMB) Liquid Substrate System MG-F Ampliflu Red BioChemika, for fluorescence D4418-5SET SIGMAFAST 3,3 -Diaminobenzidine tablet D0426-5SET SIGMAFAST DAB with Metal Enhancer tablet Clonagem, Expressão e Purificação de Proteínas Rec Vetores de Expressão E UG pflag-ats Expression Vector - 10 µg (tac promoter) E UG pflag-ctc Expression Vector - 10 µg (tac promoter) P UG pt7-flag -1 Expression Vector - 10 µg P UG pt7-flag -4 Expression Vector - 10 µg E UG ptac-mat-tag -1 Expression Vector - 10 µg E UG ptac-mat-tag -2 Expression Vector - 10 µg E UG pt7-mat-tag -1 Expression Vector - 10 µg E UG pt7-mat-tag -2 Expression Vector - 10 µg E UG p3xflag-cmv -10 Expression Vector - 20 µg 1, E UG p3xflag-cmv -9 Expression Vector - 20 µg 1, C UG pcmv-mat-tag -FLAG -1 Expression Vector - 20 µg 1, E UG pbicep-cmv -3 Expression Vector - 20 µg 2, T UG ppolh-mat -1 Transfer Vector, fusion proteins in insect cells - 20 µg 1, Meios de Cultura e seus Componentes L G LB Agar, Powder g L2897-1KG LB Agar, Powder - 1 Kg L G Luria Agar, Powder g L3147-1KG Luria Agar, Powder - 1 Kg Y G YPD Agar, Powder g Y1500-1KG YPD Agar, Powder - 1 Kg L G Luria Broth, Powder g L3522-1KG Luria Broth, Powder - 1 Kg L7658-6X500ML LB Broth EZMix Powder T G Terrific Broth, Modified, Powder g T0918-1KG Terrific Broth, Modified, Powder - 1 Kg T9179-2X5L Terrific Broth, Modified EZMix Powder Y G YPD Broth, Powder g Y1375-1KG YPD Broth, Powder - 1 Kg A G Select Agar g A5054-1KG Select Agar - 1 Kg

9 9 P G Bacteriological Peptone g P0556-1KG Bacteriological Peptone - 1 Kg T G Tryptone g T9410-1KG Tryptone - 1 Kg Y G Select Yeast Extract g Y0875-1KG Select Yeast Extract - 1 Kg S X5ML SOC Medium Antibióticos, Indutor, Substratos e Agentes Seletivos A0166-5G Ampicillin sodium salt, cell culture tested, powder K1377-1G Kanamycin sulfate, cell culture tested, powder I6758-1G IPTG - 1 g I6758-5G IPTG - 5 g B MG X-Gal - 10 mg (100 mg / ml) B MG X-Gal mg (100 mg / ml) B3928-5ML Blue-White Select Screening Reagent (40mg/ml X-Gal + 40mg/ml IPTG) 1, S MG S-Gal mg F G Ferric Ammonium Citrate g G ML G-418 disulfate salt solution, 50 mg/ml, cell culture tested A1720-1G G 418 disulfate salt powder, cell culture tested P MG Puromycin dihydrochloride, cell culture tested P MG Puromycin dihydrochloride, cell culture tested H MG Hygromycin B powder, cell culture and insect cell culture tested Soluções de Lise Celular e Inibidores de Proteases C ML CelLytic B Cell Lysis Reagent, for bacterial cell lysis, 10x conc - 10 ml C ML CelLytic IB Inclusion Body Solubilization Reagent - 25 ml C ML CelLytic M Cell Lysis Reagent, for Mammalian cell - 50 ml R ML RIPA Buffer - 50 ml C ML CelLytic P Cell Lysis Reagent, for Plant - 50 ml C ML CelLytic Y Cell Lysis Reagent, for Yeast - 50 ml P2714-1BTL Protease Inhibitor Cocktail, lyophilized (100 ml após rec) P9599-1ML Protease Inhibitor Cocktail for plant, DMSO solution - 1 ml P8849-1ML Protease Inhibitor Cocktail for Histidine-tagged proteins, DMSO solution - 1 ml P8215-1ML Protease Inhibitor Cocktail for fungal and yeast, DMSO solution - 1 ml P8340-1ML Protease Inhibitor Cocktail for mammalian cell, DMSO solution - 1 ml Purificação usando HIS-Select P6611-5ML HIS-Select Nickel Affinity Gel - 5 ml (15 mg/ml binding) P ML HIS-Select Nickel Affinity Gel - 25 ml H8162-5ML HIS-Select Cobalt Affinity Gel - 5 ml (15 mg/ml binding) H ML HIS-Select Cobalt Affinity Gel - 25 ml 1, H EA HIS-Select Spin Columns - 10 colunas (150 ug / coluna) H9913-1EA HIS-Select ilap 5 ml Column (lysis and purification) - 2 mg / coluna I G Imidazole Reagent g Purificação usando FLAG F MG ANTI-FLAG antibody, produced in rabbit ug 1, A2220-1ML ANTI-FLAG M2 Affinity Gel 1,746.00

10 10 biomapping F2426-1ML EZview Red ANTI-FLAG M2 Affinity Gel 1, M8823-1ML Biostock Anti-FLAG Sigma-Aldrich M2 Magnetic Beads 1, Remoção de Sítios de Fusão FLAG e MAT E UN Enterokinase - 50 u PRKE-1KT Enterokinase Removal Kit - ( 1,5 ml Anti-Enterokinase-Agarose; 10 preps) 1, F UG Factor X Activated (Xa) T UN Thrombin from bovine plasma, lyophilized RECOMT-1KT Thrombin CleanCleave Kit ( 1 ml Thrombin-Agarose; 10 preps) Análise Estrutural e Protein Arrays T6567-5X20UG Trypsin, Proteomics Grade, Dimethylated (modified) ug PP0100-1KT Trypsin Profile IGD Kit aplicações TT EA Trypsin Spin Columns, Proteomics Grade - 10 colunas 1, UPS1-1KT Universal Proteomics Standard Set (48 proteins) 2, MSCAL1-1KT ProteoMass Peptide and Protein MALDI-MS Calibration Kit 1, KT-F Crystallization Basic Kit for Proteins 1, GRAA2-1KT Panorama Antibody Microarray - Gene Regulation I Kit (112 proteins); 2 slides 2, CSAA1-1KT Panorama Antibody Microarray - Cell Signaling Kit (224 proteins); 2 slides 4, Epigenética ( Imprinting Genômico ) MOD50-1KT Imprint DNA Modification Kit - 50 r MDQ1-48RXN Imprint Methylated DNA Quantification Kit 1, Reagentes Ultrapuros A G Agarose, Analytical Grade g A G Agarose, Analytical Grade g 1, A G Agarose, Low Melting Point - 10 g A G Agarose, High resolution - 25 g A G Agarose Type I, Low EEO g A G Agarose Type I, Low EEO g 1, A G Agarose, for pulsed field electrophoresis - 25 g T G Trizma base g T G Trizma base - 500g B G Boric acid g B6768-1KG Boric acid - 1 kg E G EDTA, disodium salt dihydrate g T G Trizma acetate - 25 g T G Trizma acetate g A ML Acetic acid ml T9650-1L Tris Acetate-EDTA (TAE) buffer, 10x concentrate - 1 L T4415-1L Tris-Borate-EDTA (TBE) buffer, 10x concentrate - 1 L T G Trizma hydrochloride g T G Trizma hydrochloride g G G Glycine g 72.00

11 11 G G Glycine g A G Acrylamide g A G Acrylamide g M G N,N -Methylenebisacrylamide - 25 g M G N,N -Methylenebisacrylamide g T ML N,N,N,N -Tetramethylethylenediamine (TEMED) - 25 ml A G Ammonium persulfate - 25 g A G Ammonium persulfate g L G Sodium dodecyl sulfate (SDS) g U G Urea g U5378-1KG Urea - 1 kg M ML 2-Mercaptoethanol - 25 ml M ML 2-Mercaptoethanol ml S G Sodium chloride, Molecular Grade g S3014-1KG Sodium chloride, Molecular Grade - 1 kg S7277-1KG Sodium bicarbonate, Molecular Grade - 1 kg P G Potassium phosphate monobasic, Molecular Grade g C G Cesium chloride - 50 g G ML Glycerol ml G ML Glycerol ml F ML Formamide ml D ML N,N-Dimethylformamide ml D9779-1G DL-Dithiothreitol (DTT) - 1 g D9779-5G DL-Dithiothreitol (DTT) - 5 g D G Dextran sulfate, sodium salt - 10 g P1585-1MG Phorbol - 1 mg P G Poly(ethylene glycol), PEG g P ML TWEEN ml T ML Triton X ml F2637-5G Ficoll - 5 g I ML IGEPAL CA-630 (NP-40) - 50 ml H G HEPES - 25 g H G HEPES g M G MOPS - 25 g M G MOPS g D2532-5ML Denhardt s Solution 50x Concentrate - 5 ml W4502-1L Water, Molecular Grade - 1 L

12 Maiores Informações: Pedidos/Atendimento ao Cliente (Novos pedidos e dúvidas sobre pedidos existentes) Web: sigma-aldrich.com Tel: (11) (São Paulo, capital) (demais localidades) Fax: (11) Ou nosso Representante de Vendas em sua região. Produtos Sigma Life Science sob Encomenda (Oligos de DNA, sondas e sirnas) Web: sigma-aldrich.com/oligos Tel: (11) Fax: (11) Ou nosso Representante de Vendas em sua região. Serviço Técnico (Questões técnicas sobre produtos) Web: sigma-aldrich.com/techservice Tel: (11) Fax: (11) World Headquarters 3050 Spruce St. St. Louis, MO (314) sigma-aldrich.com Safety-related Information sigma-aldrich.com/safetycenter Global Locations For a list of our global locations and contact details, visit sigma-aldrich.com/international 2010 Sigma-Aldrich Co. All rights reserved. SIGMA, SAFC, SIGMA-ALDRICH, ALDRICH, FLUKA, and SUPELCO are trademarks belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P. Sigma brand products are sold through Sigma-Aldrich, Inc. JumpStart, ReadyMix, AccuTaq, eamv, GeneSnare, Extract-N-Amp, REDExtract-N-Amp, GenElute, Spectrum, PhasePrep, DirectLoad, Pulse Marker, ColorBurst, SigmaMarker, EZBlue, BlueView, SAC, SigmaFAST, pflag-ats, pflag-ctc, pt7-flag, ptac-mat, p3xflag -CMV, pcmv-mat, pbicep-cmv, ppolh-mat, EZMix, Blue-White Select, CelLytic, EZview, CleanCleave, and ProteoMass are trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. LuminoCt, REDTaq, mirpremier, BCIP, S-GAL, HIS-Select, ilap, FLAG, ANTI-FLAG, Panorama, Imprint, and Trizma are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. SYBR and SYPRO are registered trademarks of Molecular Probes, Inc. GenomePlex and TransPlex are registered trademarks of Rubicon Genomics, Inc. RNAlater and RNaseZAP are registered trademarks of Ambion, Inc. TRI Reagent is a registered trademark of Molecular Reseach Center, Inc. Immobilon is a registered trademark of Millipore, Inc. TWEEN is a registered trademark of Croda International PLC. Triton is a registered trademark of Dow Chemical Co. Ficoll is a registered trademark of GE Healthcare. IGEPAL is a registered trademark of Rhodia Operations