A value-added exopolysaccharide as a coating agent for MRI nanoplatforms

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Luca Rodrigues Oliveira
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1 Electronic Supplementary Material (ESI) for Nanoscale. This journal is The Royal Society of Chemistry Supporting Information value-added exopolysaccharide as a coating agent for MRI nanoplatforms Susana I. C. J. Palma a. Carlos. V. Rodrigues b, lexandra Carvalho c, Maria del Puerto Morales d Filomena Freitas a, lexandra R. Fernandes e,f, Joaquim M.S. Cabral b, na C.. Roque a* a UCIIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 89-6 Caparica, Portugal. b Department of ioengineering and i - Institute for ioengineering and iosciences, Instituto Superior Técnico, Universidade de Lisboa, venida Rovisco Pais, 9-, Lisbon, Portugal. c CENIMT - IN, Departamento de Ciência dos Materiais, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 89-6 Caparica, Portugal. d Departamento de iomateriales y Materiales ioinspirados, Instituto de Ciencia de Materiales de Madrid, ICMM/CSIC, 89 Madrid, Spain e UCIIO, Departamento Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 89-6 Caparica, Portugal. f CQE, Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, 9- Lisboa, Portugal. Corresponding uthor * cecilia.roque@fct.unl.pt (na C.. Roque).

2 Supporting Figures and table 8 Magnetisation (emu/gr Fe O ) ,,,,,,, pplied Field (T) Figure S. Detail of and magnetization loops. Magnetization (relative units) FC ZFC FC ZFC Temperature (K) Figure S. Temperature dependent zero-field cooling (ZFC) and field cooling (FC) magnetization measurements were performed by cooling the samples to K under a zero or a. T magnetic field, respectively. Then, the magnetization was measured while the samples were heated ( K/min) to K under a. T field. Curves for are represented in blue and curves for are represented in green.

3 Z-verage (nm) 8 6 Dispersant: PS ( mm; mm NaCl) Polydispersity Index Z-verage (nm) 8 6 Dispersant: Culture medium with FS Polydispersity Index C Time (h). D Time (h). Zeta Potential (mv) Zeta Potential (mv) Figure S. Variation of hydrodynamic diameter, polydispersity index and zeta potential of and when are they dispersed in phosphate buffered saline (PS, mm, mm NaCl, ph 7.) ( and C) or in DMEM culture medium with fetal bovine serum (FS) ( and D). Measurements were taken immediately after adding the particles to the dispersants and after h of incubation in those dispersants log [Fe] ReNcell HCT log [Fe] C log [EPS] Figure S. Effect of (), () and (C) EPS on cell viability for HCT6 (grey symbols) after 8h of incubation and ReNcell VM (black symbols) after h of incubation plus h of recovery in fresh medium. Results are expressed in terms of log [Fe] (or

4 log [EPS]) vs viability and points are connected by a -spline to guide the eye. Results are the mean of at least independent experimental assays. HCT6 cells ReNcell VM cells internalized adsorbed internalized adsorbed %mt(fe) / cell ( -) %mt(fe) / cell ( -) Figure S. Contribution of internalized and adsorbed nanoparticles for the cellular iron found in HCT6 () and ReNcell VM () cells after labeling with or. Cell line HCT6 ReNcell VM % internalization % adsorption % internalization % adsorption Table S. Distribution of cellular iron between internalized and adsorbed fractions.

5 9 8 7 Integrated Density (ID) ID = -8.7 [Fe]+7.9 r =.997 [Fe] (ug/ml) Figure S6. Linear adjustment of signal intensity (integrated density) of ReNcell VM MRI phantom images as a function of iron concentration in added to the culture medium for labeling. The first data point (signal intensity of unlabeled cells in agarose) was not accounted for the fitting.

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