POSTER SESSIONS SUNDAY AUGUST 2

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2 POSTER SESSIONS SUNDAY AUGUST 2 2 Theme 01 Exercise Physiology Theme 02 Renal Physiology Theme 03 Neurophysiology Theme 04 Cardiovascular Physiology Theme 05 Gastrointestinal Physiology Theme 06 Celular Physiology Theme 07 Comparative Physiology Theme 08 Respiratory Physiology Theme 09 Endocrine & Metabolism Theme 10 Physiology Education THEME 01 - EXERCISE PHYSIOLOGY EFEITOS DO EXERCÍCIO FÍSICO E DO LPS NO REPARO ÓSSEO JONATAS EVANDRO NOGUEIRA 1 ; LUIZ GUILHERME DE SIQUEIRA BRANCO 2 ; JOÃO PAULO MARDEGAN ISSA 2 1.ESCOLA DE EDUCAÇÃO FÍSICA E ESPORTES DE RIBEIRÃO PRETO - USP, RIBEIRAO PRETO, SP, BRASIL; 2.FACULDADE DE ODONTOLOGIA - USP, RIBEIRAO PRETO, SP, BRASIL. O reparo ósseo é um processo que consiste na restauração dos tecidos lesados, determinado por vários eventos biológicos para se obter a cura. Pode ser facilitado por meio de enxertos, estimulação bioquímica e estimulação física; e retardado por outros agentes como a endotoxina (LPS). O exercício físico (EF) exerce efeito benéfico para o osso, porém não é conhecido seu efeito sobre a reparação óssea. Investigamos o efeito do EF sobre a ação do LPS no reparo ósseo, por meio da densitometria óssea, análise histológica quantitativa do tecido ósseo neoformado e marcadores imuno-histoquímicos, em animais sedentários e treinados. O EF consistiu no treinamento na esteira durante 4 semanas. Após o treinamento, os ratos foram submetidos à cirurgia para realização do defeito ósseo na tíbia direita e 24 horas após a cirurgia, o LPS foi administrado na dose de 100 µg/kg ou 1 ml/kg de salina, i.p. Após um período de 10 dias da cirurgia e sem EF, as tíbias direitas foram obtidas para análises. Os ratos treinados tiveram menor peso corporal do que os ratos sedentários (P<0,001). O EF exerceu efeito positivo na reparação óssea, aumentando a densidade mineral óssea (P<0,005), o conteúdo mineral ósseo (P<0,005), a neoformação óssea (P<0,005), o colágeno tipo I (P<0,05) e a expressão de osteocalcina (P<0,05). Esses parâmetros não foram afetados pela administração sistêmica de LPS. Os dados indicam que o EF exerce um importante efeito osteogênico, que é mantido durante a inflamação sistêmica aguda induzida por uma única dose de LPS RESISTANCE EXERCISE INCREASES MAXIMUM STRENGTH AND INSULIN SENSITIVITY AND REDUCES BLOOD GLUCOSE IN PINEALECTOMIZED RATS MARIANA LOPES BENITES; LEONARDO RANIEL FIGUEIREDO; THAÍS SAORI TSOSURA; NATALIA FRANCISCO SCARAMELE; MURILO CARETA GUIMARÃES; RENATO FELIPE PEREIRA; FERNANDO YAMAMOTO CHIBA; DORIS HISSAKO SUMIDA UNESP, ARACATUBA, SP, BRASIL. Introduction: Insulin helps in protein syntesis, repair of damaged fibers and growth of skeletal muscles after physical exercise. Insulin action may be improved by melatonin (ME). Individuals exposed to light in nighttime inhibit the synthesis and secretion of ME by pineal gland. Objective: It was evaluated the effect of pinealectomy and resistance exercise (RE) on the maximal strenght, blood glucose and insulin sensitivity in rats. Methods: The animals were divided into 4 groups: sedentary; exercited (in ladder for 8 weeks); sedentary pinealectomized (pinealectomy performed at 40 days of life); pinealectomized exercited. All the animals were subjected to the inicial and final maximal strenght test. The exercited animals performed maximal strenght test every 15 days. The training consisted of 3 weekly sessions of 9 climbs ladder with 60% of the maximum load and 120 seconds of interval. After 8 weeks, blood samples were collected from fasted animals to perform the insulin tolerance test. Results: The pinealectomy increased blood glucose levels of sedentary animals (p<0,05). RE decreased blood glucose levels of pinealectomised animals (p<0,05) and increased the maximum strenght and sensitivity to insulin in pinealectomized and non pinealectomized animals (p<0,05). Conclusion: It was observed that, resistance exercise practice was able to increase muscle strenght and insulin sensitivity in rats, with or without pienal gland, and prevent hyperglyemia observed in pinealectomized animals RECOVERY OF FORCE PRODUCTION IS RELATED TO CD14+CD16- PHENOTYPE AND ANTICIPATED P47PHOX EXPRESSION IN BLOOD MONOCYTES OF INDIVIDUALS SUBMITTED TO ECCENTRIC EXERCISE INDUCED MUSCLE DAMAGE HENVER SIMIONATO BRUNETTA; VILMA SIMÕES PANZA; CAROLINA DE QUADROS CAMARGO; BRUNO MONTEIRO DE MOURA; RAPHAEL LUIZ SAKUGAWA; AMANDA MARREIRO BARBOSA; THAYZ RODRIGUES CHAGAS; MARINA VIEIRA DE OLIVEIRA; FERNANDO DIEFENTHAELER; EDSON LUIZ DA SILVA; EVERSON ARAÚJO NUNES FEDERAL UNIVERSITY OF SANTA CATARINA, FLORIANOPOLIS, SC, BRASIL. This study evaluated the phenotype and expression of the p47 phox protein (a NADPH oxidase subunit) in blood monocytes and its relation to the force production capacity during the recovery from skeletal muscle damage induced by eccentric exercise. Fourteen male, aged 24±3 years, body mass 72.3±10.8kg and body fat of 15.3±4.5% participated in the study. The muscle damage protocol consisted of three sets of 20 repetitions with intervals of 2 min using the elbow flexor, eccentric phase only, at a isokinetic dynamometer at 45 s -1. Blood and peak torque were collected and assayed immediately before (baseline (B)), 24, 48 and 72h after the damage protocol. Part of the individuals (n=7) recovered more than 80% (Rec>80%) of the force generation capacity (N m) in 72h after the damage (B=65.9±10.9 vs. 72h=58.9±8.4). Another group (Rec<80%) (n=7), did not reach 80% recovery (B=72.5±8.4 vs. 72h=50.1±8.9) and presented lower baseline percentage of CD14 + CD16 - monocytes than the Rec>80% group (90.2±5.4% vs. 95.9±1.6%, p<0.05). There was an increase in the expression of p47 phox in CD14 + cells in the Rec>80% 24h after damage (p<0.05). In the Rec<80%, this increment occurred at 48h and 72h, but only compared to the 24h. The increased prevalence of CD14 + CD16 - cells and the anticipated increase in p47 phox expression seem to contribute to the early recovery of force generation capacity after eccentric damage AEROBIC TRAINING PREVENTS IMPAIRMENT IN VASCULAR REACTIVITY IN RATS UNDER HIGH RISK OF METABOLIC SYNDROME DEVELOPMENT TAMIRIS MIKALOSKI BUENO GOMES; RAQUEL KINDLOVITS; RENATA FRAUCHES MEDEIROS; THAIANE GADIOLI GAIQUE; THAIS BENTO-BERNARDES; NADIA ALICE VIEIRA MOTTA; FERNANDA CARLA FERREIRA BRITO; KAREN JESUS OLIVEIRA; ANTONIO CLAUDIO LUCAS DA NÓBREGA UNIVERSIDADE FEDERAL FLUMINENSE, NITEROI, RJ, BRASIL. It s not totally elucidated the mechanism through which the aerobic training could prevent the clinical disease appearance. So the purpose was to investigate the mechanism of aerobic training on vascular reactivity of rats under high risk of metabolic syndrome development. Wistar rats, males, adults, were divided in Control group (C; drinking water), Fructose group (F; water with 10% fructose), both for 10 weeks; Control Training group (CT) and Fructose Training group (FT), adding aerobic training on a treadmill in the last 8 weeks. Triacylglycerol and malondialdehyde was higher only in F group and was similar between FT, CT, and C groups. NO was higher in trained groups when compared to sedentary groups. In addition, the F group exhibited increased vasoconstrictory reactivity to phenylephrine when compared to C and FT. The F group also presented an impaired endothelial vasodilator response to acetylcholine compared to C and FT vasodilatory response was similar to C and CT groups. What concerns about enos quantification it was observed that F group had lower expression when compared to C group and the groups that trained had higher values when compared to their sedentary controls, there was no difference between CT and FT groups. In relation to enosp it was observed that the trained groups (P=0.03) had higher values when compared to sedentary groups. So aerobic exercise prevents damage in vascular reactivity, increases enos and enosp expression in this model THE PHYSICAL EXERCISE-INDUCED CUTANEOUS HEAT LOSS DEPENDS ON THE NONTHERMAL ACTIVATION OF SYSTEMIC TRVP1 CHANNELS ANA CANCADO KUNSTETTER; WILLIAM COUTINHO DAMASCENO; DANUSA DIAS SOARES; NILO RESENDE VIANA LIMA; SAMUEL PENNA WANNER UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL. OBJECTIVE: The transient receptor potential channel - vanilloid subtype 1 (TRPV1) is a polymodal channel activated by noxious heat, capsaicin, and protons. The activation of systemic TRPV1 channels triggers cutaneous heat loss during exercise; however, it has not been determined which mode of TRPV1 activation is involved in this thermoregulatory response and, therefore, this was the objective of the present study. METHODS: The study was approved by a local ethics committee (protocol 076/2011). Male adult Wistar rats were injected with one of the following three TRPV1 antagonists: AMG517 (60 g/kg iv) or ABT-102 (3 mg/kg iv), which block all modes of TRPV1 activation, or A (3 mg/kg iv), which does not block the proton mode of TRPV1 activation. The rats were then subjected to an incremental-speed exercise on a treadmill under temperate conditions (24 C) and their brain and tail skin temperatures were measured while they were running. RESULTS: Compared with the control experiments, the acute injection of AMG 517 or ABT-102 increased the cutaneous heat loss threshold by ~1 C, exaggerating the brain hyperthermia induced by exercise. However, the injection of A did not change either the cutaneous heat loss threshold or the exercise-induced increase in brain temperature. CONCLUSION: The activation of systemic TRPV1 channels by a nonthermal stimulus increases cutaneous heat loss, thereby avoiding exaggerated brain hyperthermia during exercise REMOTE ISCHEMIC PRECONDITIONING IMPROVES RAPID ONSET VASODILATION TO SINGLE SKELETAL MUSCLE CONTRACTION IN HUMANS DANIEL ELIAS DE JESUS MANSUR 1 ; THALES COELHO BARBOSA 1 ; THIAGO RIBEIRO LOPES 2 ; LAURO CASQUEIRO VIANNA 3 ; ANTONIO CLAUDIO LUCAS DA NÓBREGA 1 ; BRUNO MOREIRA SILVA 2 1.FLUMINENSE FEDERAL UNIVERSITY, NITEROI, RJ, BRASIL; 2.FEDERAL UNIVERSITY OF SÃO PAULO, SÃO PAULO, SP, BRASIL; 3.UNIVERSITY OF BRASILIA, BRASILIA, DF, BRASIL. Remote ischemic preconditioning (RIPC) affects vascular function at rest and flow-mediated dilation. We investigated the effect of RIPC on the hyperemic response after single skeletal muscle contraction. Twelve men (25±1 years) underwent RIPC (3 cycles of 5 min occlusion/ 5 min reperfusion of the legs; thigh cuffs inflated to 200 mmhg) and a control procedure (CT; 10 mmhg) in random order on two visits. After each procedure, they performed brief (>1 s) static handgrip contractions, in supine position with 2-min interval. Beat-to-beat blood pressure (BP), forearm blood flow (FBF) and vascular conductance (FVC; FBF/BP) before (BL) and for 15 cardiac cycles after each contraction were averaged from 3 contractions. Muscle contractions increased FBF and FVC within 3 to 4 cardiac cycles (62% and 60% change from BL, respectively FBF and FVC). Peak increase in FBF and FVC was similar between RIPC and CT (P > 0.05), but the following decay was attenuated by RIPC, such that FBF was ~12% higher than CT between the 9th-15th cardiac cycles (P < 0.05 vs. CT) and FVC was ~15% higher than CT between the 8th-15th cardiac cycles (P < 0.05 vs. CT). Our findings demonstrate that RIPC prolongs the vasodilator effect of a single contraction EFEITO AGUDO DA ESTIMULAÇÃO TRANSCRANIANA DE CORRENTE CONTÍNUA NO NÚMERO TOTAL DE REPETIÇÔES E PERCEPÇÃO DE FADIGA MUSCULAR RODRIGO CUNHA DE MELLO PEDREIRO 1 ; EDUARDO LATTARI 2 ; MARIA LÚCIA ANDRADE 1 ; ALBERTO SOUZA DE SÁ FILHO 2 ; ANTÔNIO MARCOS MOURA 2 ; SAMARA SEZANA COSTA 1 ; GERALDO ALBUQUERQUE MARANHÃO NETO 1 ; SÉRGIO MACHADO 2 1.UNIVERSIDADE SALGADO DE OLIVEIRA, CORDEIRO, RJ, BRASIL; 2.UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL. O objetivo do estudo foi avaliar o efeito agudo da estimulação transcraniana de corrente continua anódica (ETCC-a) sobre o volume total de repetições e percepção subjetiva do esforço em indivíduos praticantes de treinamento força (TF). A amostra foi de 10 sujeitos treinados. Os mesmos foram submetidos a exercício de flexão de cotovelo na barra com carga específica de 10RM, imediatamente após, responderam a escala OMNI-RES e foram estimuladas durante 20 minutos com um protocolo ETCC, dependendo da randomização. Após a ETCC, novamente foram submetidos a realizar o mesmo exercício e responder a escala OMNI-RES. Foram três condições experimentais, a ETCC catódica (ETCC-c), ETCC-a e ETCC placebo (ETCC-p). Houve um intervalo de 48 a 72 horas entre as visitas. Uma interação de condições e tempo (F = 52,395; p 0,001) mostrou que repetições completadas após ETCC-a foram maiores em comparação com as outras condições realizadas também após. Em relação à percepção de esforço, a ANOVA de duas entradas para medidas repetidas mostrou uma interação entre condição e tempo (F = 28,445; p 0,001), onde o esforço percebido foi diminuído após a condição ETCC-a e aumentada após a condição ETCC-c. Em suma, parece ser benéfico realizar uma sessão de 20 minutos ETCC-a para potencializar o rendimento de indivíduos praticantes de TF, assim como reduzir a percepção de fadiga EFFECT OF ISCHEMIC PRECONDITIONING ON AEROBIC PERFORMANCE IN HIGH-LEVEL RUNNERS: A RANDOMIZED AND PLACEBO/NOCEBO CONTROLLED STUDY JEANN LUCCAS DE CASTRO SABINO-CARVALHO 1 ; THIAGO RIBEIRO LOPES 2 ; THIAGO HENRIQUE NUNES FERREIRA 1 ; TIAGO OBEID-FREITAS 1 ; JOSE ERNESTO SUCCI 1 ; ANTONIO CARLOS SILVA 1 ; BRUNO MOREIRA SILVA 1 1.UNIVERSIDADE FEDERAL DE SÃO PAULO, SAO PAULO, SP, BRASIL; 2.CENTRO OLÍMPICO DE TREINAMENTO E PESQUISA, SÃO PAULO, SP, BRASIL. It has been reported that ischemic preconditioning (IPC; brief cycles of ischemia) enhances aerobic performance. But, placebo/nocebo effects have not been adequately controlled. Moreover, no study assessed high-level runners or used strict criteria to confirm the maximal oxygen uptake (VO2max). Thus, we submitted 11 high-level runners to three interventions, in random order (IPC; SHAM, fake ultrasound session; CT, resting control). Subjects were told that both IPC and SHAM would improve performance compared to CT (i.e., we sought to induce a similar placebo, while avoiding a nocebo effect). After each intervention, gas exchange, blood lactate [La] and rating of perceived exertion (RPE) were measured during a maximal incremental test. Ten min later, a supramaximal constant test was used to verify the VO2max. Most subjects believed at pre-test that IPC and SHAM would improve performance (73 and 82%, respectively). Running oxygen cost (P = 0.80), VO2max (P = 0.44), RPE (P = 0.42) and lactate threshold (P = 0.57) were similar among interventions. Peak velocity in the maximal test (P = 0.28) and time to exhaustion in the supramaximal (P = 0.34) were not altered. In summary, these preliminary results indicate that IPC did not improve aerobic performance in high-level runners. Thus, the ergogenic effect reported by previous studies in intermediate-level runners suggests that aerobic fitness may modulate the IPC effect on aerobic performance THE INCREASE IN GUT PERMEABILITY INDUCED BY EXERCISE DEPENDS ON THE MAGNITUDE OF HYPERTHERMIA ALEXANDRE SERVULO RIBEIRO HUDSON; ANNE DANIELI NASCIMENTO SOARES; CRISTIANO ANTÔNIO MACHADO-MOREIRA; DANUSA DIAS SOARES; VALBERT NASCIMENTO CARDOSO; CÂNDIDO CELSO COIMBRA; SAMUEL PENNA WANNER UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL. Gut permeability increases as a consequence of exercise-induced hyperthermia, leading to endotoxin leakage and an inflammatory response. In more severe cases, endotoxemia can cause multiple-organ failure and death. Thus, we investigated whether the magnitude of hyperthermia

3 induced by prolonged exercise affects intestinal permeability. Twenty-nine male Wistar rats were divided in four groups: rest at 24 C or constant-speed treadmill running (90 min, 15 m/min) at 13ºC (cold), 24 C (temperate) and or 31 C (warm). Thirty min prior to the experiments, the rats received 0.1 ml of diethylenetriaminepentaacetic acid solution labeled with 18.5 MBq of 99mTc by gavage. After the experiments, radioactivity was counted in blood samples and used as an index of intestinal permeability. The abdominal temperature of the rats was not changed by constant exercise at 13 C, but attained average values of ± 0.35 C at the end of exercise at 24 C. No changes in the intestinal permeability were observed between the exercise groups at 13 C and 24 C and the rest group at 24 C. The rats subjected to exercise at 31 C showed abdominal temperature values that exceeded 40 C and remained high for a prolonged period. In this group, intestinal permeability was higher than in the other three groups. Therefore, the magnitude of hyperthermia affects exercise-induced intestinal permeability, which was only observed when rats showed abdominal temperatures exceeding 40ºC EFFECT OF DIFFERENT TYPES OF AEROBIC TRAINING ON PHYSICAL PERFORMANCE AND THERMOREGULATORY RESPONSES IN RATS SUBJECTED TO AN INCREMENTAL EXERCISE IN THE HEAT FRANCISCO TEIXEIRA COELHO; FILIPE FERREIRA VAZ; CLETIANA GONÇALVES DA FONSECA; WASHINGTON PIRES; DANUSA DIAS SOARES; SAMUEL PENNA WANNER UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL. Aerobic training induces several adaptations in the thermoregulatory system, including a decrease in the core body temperature threshold for cutaneous vasodilation during exercise, which may improve human performance in a hot environment. This study investigated the effect of different types of aerobic training conducted in a temperate environment on the performance and thermoregulatory responses of rats during an exercise in the heat. Adult male Wistar rats (n = 32) were divided into four groups: a control group (C) and three groups that were subjected to a 8- week aerobic training, during which the physical overload was achieved by predominantly increasing the exercise intensity (I), duration (D) or by increasing both (ID). After the training protocol, the core and tail skin temperatures of the rats were measured during an incremental treadmill running (initial speed of 10 m/min, which was increased by 1 m/min every 3 min until volitional fatigue) in a hot environment (32 C). All groups subjected to the aerobic training showed improved performance (I: 48±3 min; D: 47±4 min; ID: 46±2 min; p < 0.05) compared with the C group (34±2 min). There were no differences in the core and tail temperatures among the groups studied. These findings suggest that the three types of training increased aerobic performance in the heat by mechanisms that were independent of thermoregulatory adaptations CHRONIC AEROBIC EXERCISE RESTORE MICROVASCULAR FUNCTION IN RATS WITH METABOLIC SYNDROME ROBERTA FRANCO BARBOSA PEÇANHA; FLAVIA GARCIA DE OLIVEIRA; MARCUS VINICIUS MACHADO; ELIETE DALLA CORTE FRANTZ; ALESSANDRA CHOQUETA DE TOLEDO ARRUDA; ANTONIO CLAUDIO LUCAS DA NÓBREGA UFF, NITEROI, RJ, BRASIL. Introduction: Metabolic syndrome (MS) is related to systemic functional microvascular alterations including a significant reduction in microvessel density. Aim: The aim of the study was to investigate the effects of exercise training on functional capillary density in the skeletal muscle and skin of rats with MS. Methods: Male Wistar-Kyoto rats were fed a standard commercial diet (CON) or high-fat diet (HFD) for 32 weeks. Animals receiving the HFD were randomly divided into sedentary (HFD+SED) and training groups (HFD+TR) at the 20th week. After 12 weeks of aerobic treadmill training, the maximal oxygen uptake (VO2max); hemodynamic, biochemical and anthropometric parameters; and functional capillary density were assessed. Results: Exercise training increased the VO2max (p<0.05) and exercise tolerance (p<0.05) compared with the HFD+SED and CON. The HFD+TR also reduced visceral fat (p<0.001) and systolic blood pressure (127±2 vs. 150±2 mmhg, p<0.001). Moreover, exercise improved glucose tolerance, as evaluated by an intraperitoneal glucose tolerance test (p<0.05), fasting plasma glucose (p<0.001) and fasting plasma insulin levels (p<0.05) compared with HFD+SED. Exercise training also increased the number of spontaneously perfused capillaries in the skeletal muscle (252±9 vs. 207±9 capillaries/mm2) compared with HFD+SED. Conclusion: Exercise training reversed skeletal muscle and skin capillary rarefaction in our experimental model of MS and obesity. Key words: Metabolic syndrome; Exercise; Microvascular density AEROBIC EXERCISE MODULATION OF MENTAL STRESS-INDUCED RESPONSES IN CULTURED ENDOTHELIAL PROGENITOR CELLS FROM HEALTHY AND METABOLIC SYNDROME SUBJECTS HELENA NALY MIGUENS ROCHA 1 ; NATÁLIA GALITO ROCHA 1 ; ALLAN ROBSON KLUSER SALES 1 ; BRUNO MOREIRA SILVA 2 ; ALINE ARAÚJO RABELO 1 ; ANTONIO CLAUDIO LUCAS DA NÓBREGA 1 1.UNIVERSIDADE FEDERAL FLUMINENSE, NITEROI, RJ, BRASIL; 2.UNIVERSIDADE FEDERAL DE SÃO PAULO, SÃO PAULO, SP, BRASIL. Studies have shown that exercise acutely prevents the reduction in flow-mediated dilation induced by mental stress in metabolic syndrome (MetS). We investigated whether exercise protects from the deleterious effect of mental stress on cultured endothelial progenitor cells (EPC) in healthy subjects and those with MetS. Ten healthy subjects (aged 31±2) and ten subjects with MetS (aged 36±2) underwent a mental stress test, followed by either 40 min of leg cycling or rest across two randomized sessions: mental stress+non-exercise control (MS) and mental stress+exercise (MS+EXE). Stroop Color-Word test was used to elicit mental stress. Blood samples were drawn at baseline and following sessions to isolate mononuclear cells. They were cultured in fibronectincoated plates for seven days, and EPC were identified by immunofluorescence (acldl+/uea-1 Lectin+). All subjects presented similar increases in mean blood pressure and heart rate during mental stress test (P<0.01) in both the MS and MS+EXE. EPC number was not different between groups at baseline in both sessions (P>0.05). EPC response to MS and MS+EXE was increased in healthy subjects, whereas it was decreased in subjects with MetS (P<0.04). In healthy subjects, the EPC response to MS+EXE was greater than the response to MS alone (P=0.03). An exercise session increased EPC in healthy subjects but did not prevent the EPC reduction induced by mental stress among subjects with MetS HEPATIC RENIN ANGIOTENSIN SYSTEM IS MODULATED BY EXERCISE TRAINING IN FRUCTOSE-FED RATS JULIANA BITTENCOURT SILVEIRA DE LIMA; ISABELE GOMES GIORE; ELIETE DALLA CORTE FRANTZ; RENATA FRAUCHES MEDEIROS; THAIS BENTO-BERNARDES; THAIANE GADIOLI GAIQUE; CAROLINE FERNANDES SANTOS; KAREN JESUS OLIVEIRA; ANTONIO CLAUDIO LUCAS DA NÓBREGA UNIVERSIDADE FEDERAL FLUMINENSE, NITEROI, RJ, BRASIL. Introduction: The renin-angiotensin system (RAS) has been implicated in the development of nonalcoholic fatty liver disease (NAFLD). We investigated whether the hepatic RAS is modulated by exercise training and if this modulation improves NAFLD. Methods: Male Wistar rats were divided into (n = 8 each) control (CT), exercise control (CT-Ex), high fructose (HFr) and exercise high fructose (HFr-Ex; fructose in drinking water) groups. After two weeks, CT-Ex and HFr-Ex rats were assigned to a treadmill training protocol at moderateintensity for 8 weeks (60 min/day, 4 days/week). We assessed body mass, lipid metabolism, hepatic histopathology and the expression profile of components of the RAS. The differences between the groups were tested by ANOVA and the post hoc Holm Sidak test. Results: Neither fructose overload nor exercise training influenced body mass gain. Exercise training was effective in preventing hepatic steatosis as well as local and systemic triglyceride accumulation. The HFr rats displayed increased hepatic ACE protein expression which was attenuated by exercise training. Exercise training restored the ACE2/Mas receptor axis and decreased the ACE/ACE2 ratio and the AT1R/Mas receptor ratio towards normal levels in liver. Conclusion: Exercise training may favor the counter-regulatory ACE2/Mas receptor axis over the classical RAS (ACE/AT1R axis), which could be responsible for the prevention of NAFLD. Key words: Renin-angiotensin system (RAS); Exercise training; Fructose; Non-alcoholic fatty liver disease (NAFLD) ROLE OF AEROBIC TRAINING ON LEFT VENTRICULAR HYPERTROPHY IN A HIGH FRUCTOSE EXPERIMENTAL MODEL RAQUEL KINDLOVITS; TAMIRIS MIKALOSKI BUENO GOMES; RENATA FRAUCHES MEDEIROS; KAREN JESUS OLIVEIRA; ANTONIO CLAUDIO LUCAS DA NÓBREGA UNIVERSIDADE FEDERAL FLUMINENSE, NITEROI, RJ, BRASIL. The high fructose intake is associated with ventricular hypertrophy, while training is responsible for physiological hypertrophy. However, it is not known if training can prevent this alteration caused by high fructose diet. Thus, the purpose of this study was to investigate the effect of aerobic training on ventricular hypertrophy alterations caused by a high fructose intake. Wistar rats, males, adults, were divided in Control group (C; drinking water), Fructose group (F; water with 10% fructose), both for 10 weeks; Control Training group (CT) and Fructose Training group (FT), adding aerobic training, in the last 8 weeks. At the end of treatment metabolic variables, body lipid, left ventricle (LV) weigh and PTP1B protein expression in LV were analyzed. Triglycerides (TG), isoprostane and insulin were higher only in F group and were similar between others groups. This same pattern was observed in relation to lipid body content. In addition, CT, F and FT groups presented higher LV weight when compared to C group. The maximal aerobic capacity was lower in F group compared to others. The PTP1B protein expression, responsible for inhibit insulin signaling pathway, had tendency to be higher (47%) on F group, when compared to C group, however C and FT groups demonstrated similar values. In conclusion, though aerobic training increased LV weight, it was able to prevent alterations in body lipid content, maximal aerobic capacity, insulin, TG, oxidative stress and in PTP1B expression EFFECTS OF ACUTE SUPPLEMENTATION WITH GLUTAMINE ON PHYSICAL PERFORMANCE IN A PROLONGED EXERCISE IN THE HEAT DIOGO ANTONIO SOARES PACHECO 1 ; DANUSA DIAS SOARES 2 ; IULA LAMOUNIER LUCCA 1 ; MARCELO TEIXEIRA DE ANDRADE 1 ; ALEXANDRE SERVULO RIBEIRO HUDSON 1 ; YGOR ANTONIO TINOCO MARTINS 1 ; WESLLEY MOURA SANTOS 1 ; NICOLAS HENRIQUE SANTOS BARBOSA 1 ; CRISTIANO ANTÔNIO MACHADO-MOREIRA 3 ; CÂNDIDO CELSO COIMBRA 1 ; SAMUEL PENNA WANNER 1 1.FEDERAL UNIVERSITY OF MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSIDADE FEDERAL DE MINAS GERAIS, MINAS GERAIS, MG, BRASIL; 3.FEDERAL UNIVERSITY OF JUIZ DE FORA, GOVERNADOR VALADARES, MG, BRASIL. This study evaluated the effects of acute supplementation with glutamine (GLN) on physical performance in a prolonged exercise in the heat. Nine healthy subjects participated in the study. On the first visit to the laboratory, a maximum progressive test was conducted to measure the maximum power output (PMAX; 210 ± 30 W) attained by the volunteers, followed by a familiarization exercise trial in the heat. In the experimental trials, volunteers were supplemented with GLN, glycine (GLY) or a placebo drink (PLA) and then exercised until volitional fatigue (started cycling at 50% of PMAX, which was increased by 1% every 8 min) in a hot environment (34 C; 63% RH). The supplementation with GLN induced no ergogenic effect on prolonged exercise in the heat (PLA: 68.4 ± 6.7 min vs. GLY: 68.4 ± 5.6 min vs. GLN: 69.6 ± 5.0 min; p = 0.916), although some volunteers displayed marked improvements in performance (maximum increase of 30%) and others displayed marked impairments (maximum reduction of 19%). In addition, the change in performance caused by GLN showed a negative correlation with performance in the PLA trial (r = -0.79; p < 0.01). Our data indicate that acute supplementation with GLN did not influence physical performance during cycling in the heat; although a great individual variability was observed. Moreover, we provide preliminary evidence that supplementation with GLN may induce ergogenic effects in individuals who have low performance in the heat CARDIOPULMONARY AFFERENTS MODULATE HEART RATE RESPONSIVENESS TO MUSCLE METABOREFLEX ACTIVATION IN HUMANS MAYARA C SOUZA 1 ; JAMES P FISHER 2 ; LAURO CASQUEIRO VIANNA 1 1.UNIVERSITY OF BRASÍLIA, BRASILIA, DF, BRASIL; 2.UNIVERSITY OF BIRMINGHAM, BIRMINGHAM, REINO UNIDO. Human studies have shown that muscle metaboreflex activation-mediated increases in heart rate are principally attributable to increased cardiac sympathetic activity. The loading of cardiopulmonary baroreceptors, associated with the supine posture, also provides neuromodulatory signals to the brainstem and elicits cardiac sympathoinhibition. We sought to determine whether cardiopulmonary loading modulates the HR response to muscle metaboreflex activation in humans. In 14 healthy men (20±1 yrs) oscillometric blood pressure (Microlife 3BTO- A) and heart rate (HR; Polar RS800) were measured during post-exercise ischemia (PEI; muscle metaboreflex activation) with concurrent cardiopulmonary loading (supine posture) or unloading (seated posture). At rest, the shift from supine to seated posture increased HR from 64±2 to 69±2 beats.min-1 (P<0.05), whereas blood pressure decreased from 90±2 to 84±2 mmhg (P<0.05). PEI provoked a robust increase in blood pressure from rest ( 36±3 mmhg, P<0.05), which was not affected by body posture. During PEI with cardiopulmonary unloading, HR was elevated from rest ( 9±3 beats.min-1, P<0.05), but blunted with cardiopulmonary loading ( 0.9±2 beats.min-1, P>0.05 vs rest). Thus HR responsiveness to muscle metaboreflex activation is importantly modulated by afferent input from cardiopulmonary baroreceptors, suggesting that the cardiopulmonary baroreflex and muscle metaboreflex interact to control cardiac autonomic activity EFFECTS OF ACUTE SUPPLEMENTATION WITH GLUTAMINE ON A VIGILANCE TASK PERFORMANCE AFTER A PROLONGED EXERCISE IN THE HEAT DIOGO ANTONIO SOARES PACHECO 1 ; DANUSA DIAS SOARES 2 ; RÚBIO SABINO BRUZZI 1 ; IULA LAMOUNIER LUCCA 1 ; MARCELO TEIXEIRA DE ANDRADE 1 ; DOUGLAS SILVA BERNARDES 1 ; CHRISTIANO EDUARDO VENEROSO 3 ; CRISTIANO ANTÔNIO MACHADO-MOREIRA 4 ; CÂNDIDO CELSO COIMBRA 1 ; SAMUEL PENNA WANNER 1 1.UNIVERSIDA FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSIDADE FEDERAL DE MINAS GERAIS, MINAS GERAIS, MG, BRASIL; 3.UNIVERSIDADE FEDERAL DO MARANHÃO, SÃO LUÍS, MG, BRASIL; 4.UNIVERSIDADE FEDERAL DE JUIZ DE FORA, JUIZ DE FORA, MG, BRASIL. This study evaluated the effects of acute supplementation with glutamine (GLN) on a vigilance task performance after a prolonged exercise in the heat. Nine healthy subjects participated in the study. On the first visit to the laboratory, they performed a maximal progressive exercise test to measure the maximum power output (PMAX; 210 ± 30 W), followed by a familiarization exercise trial and a psychomotor vigilance test (PVT). In the experimental trials, the volunteers initially performed the PVT and then ingested a drink solution containing GLN or glycine (GLY) or placebo drink. One hour later, the volunteers exercised until volitional fatigue (started cycling at 50% of PMAX, which was increased by 1% every 8 min) in the heat (34 C; 63% RH) and then performed a second PVT. This task lasts 10 min and consists of pressing the mouse button as fast as possible in response to visual stimuli. We assessed reaction time, false alarm and lapse of attention of the volunteers. During the exercise trials, rectal temperature (TRET) and heart rate (HR) were recorded. The fatiguing exercise in the heat and the acute supplementation with GLN or GLY did not affect any of the parameters related to vigilance performance in PVT. Aside from this observation, there were no differences in TRET and HR among the three experimental trials. Our data indicate that prolonged exercise in the heat and acute supplementation with GLN did not affect the performance on a vigilance task TREINAMENTO FÍSICO AERÓBICO E RESISTIDO, PRÉVIOS AO INFARTO DO MIOCÁRDIO, PREVINEM ALTERAÇÕES AUTONÔMICAS E INFLAMATÓRIAS EM RATOS CATARINA DE ANDRADE BARBOZA 1 ; NICOLLE MARTINS CARROZZI 1 ; LUIZA MELANIE SILVA 1 ; JULIANA CRISTINA OLIVEIRA 1 ; LEANDRO YANASE ROCHA 1 ; DANIELE JARDIM FERIANI 1 ; MARCOS ELIAS ABSSAMRA 1 ; CRISTIANO MOSTARDA 2 ; Mª CLAUDIA IRIGOYEN 3 ; BRUNO RODRIGUES 4 1.UNIVERSIDADE SAO JUDAS TADEU, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DO MARANHÃO, SAO LUIS, MA, BRASIL; 3.INCOR-INSTITUTO DO CORACAO FMUSP, SAO PAULO, SP, BRASIL; 4.UNICAMP UNIVERSIDADE DE CAMPINAS, SAO PAULO, SP, BRASIL. O objetivo desse estudo foi comparar os efeitos do treinamento físico aeróbico (TA) e resistido (TR), realizados previamente ao infarto do miocárdio (IM), em animais. Ratos Wistar foram divididos em: controle (C), sedentário + IM (SI), TA + IM (TAI) e TR + IM (TRI). Após 2 meses de 3

4 treinamento/acompanhamento os animais foram submetidos ao IM e às avaliações autonômicas e inflamatórias do ventrículo esquerdo em ratos. A área de IM não foi modificada pelo treinamento prévio. A sensibilidade barorreflexa foi melhorada apenas no grupo TAI. Os grupos TAI (53±4 bpm) e TRI (40±4 bpm) apresentaram semelhante redução do Tônus Simpático (TS) em comparação ao grupo SI (117±6 bpm); no entanto, o Tônus Vagal (TV) foi aumentado somente nos animais TAI (52±4 bpm), se comparados com os grupos SI (23±5 bpm) e TRI (21±3 bpm). Já a FC intrínseca, reduzida no grupo SI, foi normalizada por ambos tipos de treinamento. As concentrações de citocinas no ventrículo esquerdo IFN-γ, IL-6 e TNF-α, aumentadas no grupo SI (34,1±2; 143,7±12 e 35,3±2 pg/µg prot), foram reduzidas nos grupos TAI (20,1±3; 76,0±12 e 23,6±3 pg/µg prot) e TRI (17,7±0,8; 68,7±7 e 20,6±3 pg/µg prot). Adicionalmente, a queda do TS foi correlacionada com a redução de TNF-α (r=0,74; P=0,0001). Dessa forma, é possível inferir que, independentemente do tipo, o treinamento físico pode ser uma ferramenta eficaz na prevenção das complicações funcionais e teciduais oriundas da isquemia miocárdica MODERATE HEAT STRESS INDUCES POST-EXERCISE HYPOTENSION AND INCREASES PARASYMPATHETIC RESPONSE IN SHR RATS MICHELE MACEDO MORAES; WASHINGTON PIRES; VALÉRIA ANDRADE PINTO; PATRÍCIA CONCEIÇÃO ROCHA RABELO; SAMUEL PENNA WANNER; DANUSA DIAS SOARES UFMG, BELO HORIZONTE, MG, BRASIL. In spontaneously hypertensive rats (SHR), post-exercise hypotension is mediated by reductions in peripheral resistance and sympathetic activity. As heat exposure increases sympathetic activity and MAP in response to increases in core temperature (Tc), we investigated if a single bout of exercise in warm (W) reduces MAP. 7 SHR (295±7g; 17,2±0,9wk) and 7 Wistar (WIS) (307,1±12,4g; 15±0,5wk) had a carotid catheter and a temperature sensor implanted. The rats were submitted to a treadmill exercise (50% Vmax for 30 min, 5% inclination) in temperate (T) (23.5 C) and W (30.3 C) environment. Pre and post exercise, the animals were maintained 2h at rest in the same environment as during exercise. Spectral power components were evaluated to heart rate (HR) and blood pressure (BP). MAP post-exercise reduced only for SHR (P<0.05) (T pre-exercise: 167 ± 4; post: 159 ± 4/ W pre: 154±7; post: 147±6mm Hg). In SHR, exercise in W reduced HR (pre: 320±12; post: 294±8bpm) and increased HF of HR (T pre: 4±1; post: 6±1/ W pre : 11±2; post: 11±1). BP VLF component was reduced by the exercise in T (pre: 31±4/ post: 23±3). Tc was higher in W during exercise for WIS and SHR, but not in pre or post-exercise (stabilized after 30 min of postexercise). Tail skin temperature was higher in W than in TN. In SHR, post-exercise hypotension occurs even under moderate heat stress, which can be explained, at least in part, by increases in parasympathetic response. THEME 02 RENAL PHYSIOLOGY CLONING OF NA+/K+-ATPASE SUBUNITS GENES FROM THE RODENT CERRADOMYS GOYTACA MARCOS FELIPE MARTINS GOMES; GEÓRGIA DA SILVA FELTRAN; CAROLINA AUGUSTA DE BARROS SILVA; JACKSON DE SOUZA MENEZES; JOSÉ LUCIANO NEPOMUCENO SILVA; RODRIGO NUNES DA FONSECA UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, MACAÉ, RJ, BRASIL. The Na+/K+-ATPase is a P type ATPase and acts as an electrogenic pump that promotes cellular extrusion of 3 Na+ against the entry of 2 K+, using the energy released by the hydrolysis of ATP. C.goytaca is a rodent species recently discovered in the Jurubatiba National Park Sand Bank (north of Rio de Janeiro state), an environment where fresh water is usually scarce. The aim of this study was the characterization, initially by cloning and sequencing, of α, β and γ subunit genes of renal Na+/K+-ATPase from C.goytaca. After deep anesthesia and sedation (ketamine and xylazine), renal excision was performed for total RNA extraction. 1 µg of total RNA was reversed transcribed in cdna and RT-PCR reactions were performed with primers sets designed based on multiple alignments of the 3 Na+/K+-ATPase subunits genes from different species of rodents. RT-PCR amplified products were ligated into pgem-t-easy vector and the ligation reaction was used to transform competent E. coli. Colonies carrying vectors with inserts of expected size had their plasmid DNA purified and sequenced. Sequences of fragments of approximately 1500, 950 and 210bp were obtained for subunits α, β, and γ genes, respectively. The gene fragment encoding the first half of α subunit shares 99% identity with the orthologue from Rattus norvegicus. Other sequences are presently under analysis. Conclude that the C.goytaca expresses the 3 Na+/K+- ATPase subunits in the kidney and that these genes shares high similarity with other rodents orthologues RENAL DAMAGE INDUCED BY ADRIAMYCIN: INFLUENCE OF PHYSICAL PRECONDITIONING CAMILA DE MATTOS FALEIROS; HELOÍSA D COLETTA FRANSCESCATO; MARCELO PAPOTI; LUCAS CHAVES; CLEONICE GIOVANINI; ROBERTO SILVA COSTA; TEREZILA MACHADO COIMBRA UNIVERSIDADE DE SÃO PAULO, RIBEIRAO PRETO, SP, BRASIL. Adriamycin (ADR) nephropathy is an experimental model for evaluation of the mechanisms involved in progressive renal disease. A single dose of ADR in rats induces progressive proteinuria and renal structural changes that lead to glomerular and tubulointerstitial lesions. The aim of this study was to evaluate the influence of physical preconditioning in the renal structure and function in rats treated with ADR. Male Wistar rats (n=27) previously submitted or not to physical training in a treadmill running (4 weeks) were injected with ADR (2.5 mg/kg) (PhT+ADR and ADR groups) or saline (PhT+SAL and SAL groups). Urine samples were collected 7 and 30 days after injections to quantify albumin excretion, and blood samples 60 days after treatment to evaluate plasma creatinine levels (Pcreat). The kidneys were removed for morphometric and immunohistochemical analysis. There was a progressive rise in albuminuria in AD-injected rats on the 7th and 30th days, but with less intensity in PhT+ADR (p<0.05). The Pcreat (mg%) increased in ADR group (1.43±0.45) but not in the others groups (PhT+ADR=0.78±0.36; PhT+SAL=0.83±0.12; SAL= 0.90±0.04). The increased vimentin expression and relative interstitial area in renal cortex in ADR group were attenuated in PhT+ADR group (p<0.05). Physical preconditioning can attenuate the renal lesions provoked by ADR. This effect was associated with attenuation of albuminuria and relative interstitial area increases and with maintenance of Pcreat and cortical expression of vimentin RESTRIÇÃO PROTEICA DURANTE A PUBERDADE INDUZ AUMENTO DA PRESSÃO ARTERIAL A LONGO TERMO SEM AFETAR FUNÇÃO RENAL LETICIA DINIZ CREPALDI UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGA, PR, BRASIL. A síndrome cardiometabólica tem alcançado proporções epidêmicas. Estudos demonstram que a desnutrição, em fases críticas do desenvolvimento, é um fator importante para o desenvolvimento dessa síndrome na vida adulta. Este trabalho teve como objetivo analisar parâmetros da função renal e pressão arterial de ratos Wistar que receberam dieta hipoproteica durante a puberdade. Os animais foram separados em dois grupos: G-NP (dieta normoproteica - n= 9) e G-HP (dieta hipoproteica - 4% de proteínas - n= 9). A dieta foi administrada dos 30 aos 60 dias de vida. Aos 120 dias, foi feito registro da pressão arterial, coleta de sangue, medida da ingestão hídrica e urina de 24 h, análises da proteinúria e da taxa de filtração glomerular (TFG). Foi observado redução significativa no peso corporal (G-NP=355,38 ± 21,77 e G-HP = 329,65 ± 21,10g - p=0,028), no comprimento nasoanal (G-NP = 23,69 ± 0,45 e G-HP =23,04 ± 0,66cm - p=0,025) e aumento significativo na pressão arterial média (G-NP = 95,7 ± 7,2 e G-HP = 108,3 ±8,1mmHg - p=0,01) no G-HP. No entanto, não houve alteração nem na TFG (G-NP=0,4 ± 0,10 e G-HP=0,4 ± 0,06mL/min/100g - p=0,89) nem na proteinúria (G-NP =7,04 ± 2,03 e G-HP=7,67 ± 1,76mg/24hrs - p=0,48). Concluindo, animais que receberam dieta hipoproteica, durante a puberdade, apresentaram aumento da pressão arterial na vida adulta sem alteração na taxa de filtração glomerular e proteinúria RENAL EFFECTS OF DIABETES MELLITUS INDUCED DURING PREGNANCY AND MAINTAINED IN THE POSTPARTUM PERIOD IN WISTAR RATS NATHANE FRANÇA SILVA; NATANY GARCIA REIS; PÂMELLA FRANCIS SANTOS; ANA PAULA COELHO BALBI FEDERAL UNIVERSITY OF UBERLÂNDIA (UFU), UBERLÂNDIA, MG, BRASIL. Objective: Evaluate the effects of diabetes mellitus (DM) induced in Wistar rats during pregnancy and maintained by 50 days in the postpartum period on structural and renal function. Material and methods: There were four groups (G): G1 (non-pregnant controls rats), G2 (non-pregnant diabetic rats), G3 (control mothers) and G4 (diabetic mothers). Results: systolic blood pressure were not different among the groups, but there was a tendency to reduction in glomerular filtration rate from G4 when compared to the other G. Morphometric analysis showed a reduction of total renal sectional area from G4 and an increase in G3. The glomerular area and the capsular space did not differ between G studied, but there was an increase in glomerular tuft area in G3 and G4. G2 and G4 showed higher percentage of collagen cortical. Regarding PCNA immunoreactivity, G3 showed higher number of glomerular proliferating cells while in G4 it was lower, when compared with their respective controls. Further, G4 showed higher number of proliferating cells in the tubulointerstitial (TBI) compartment. Glomerular and TBI expression of α-sma were increased in G4 compared to other G. Immunoreaction for glomerular p-p38 expression showed a reduction in G4, while p-jnk expression was higher in both the glomeruli and TBI compartment in G4 compared to other G. Conclusion: DM resulted in renal impairment on the postpartum period and these alterations may be related to changes in the expression of MAPK ACUTE MICROCISTIN-LR EXPOSURE INDUCE CHANGES IN MICE RENAL FUNCTION AND PODOCIN MRNA EXPRESSION GEÓRGIA DA SILVA FELTRAN; RODRIGO NUNES DA FONSECA; NATALIA FEITOSA MARTINS; RAQUEL MORAES SOARES; SANDRA MARIA FELICIANO DE OLIVEIRA E AZEVEDO; JACKSON DE SOUZA MENEZES.,., RJ, BRASIL. Cyanobacterias are photosynthetic prokaryotes potentially producing toxins, which can cause severe physiological damage. The aim this work was analyze changes in renal function of mice intoxicated by microcystin-lr. C57BL6 mice were divided into control (CTRL) and 35 ug/kg of microcystin-lr IP (MYCST35). After deep anesthesia and sedation, blood sample was collected (for plasma isolation) and the organs was excised. The renal function was assed using clearance methodology. The organs index were calculated by the following ratio: organ weight/body weight. The kidneys were excised for RNA extraction, using TRIZOL reagent, and cdna was synthesized. The podocin relative mrna expression was determined by qrt-pcr technique. Statistical analyzes were performed using the t test and the differences were considered significant when p<0.05. In MCYST35 group it was observed higher glucose fractional excretion (%) and clearance (ml/min/kg) (0.068± and ±0.0079) and lower GFR (ml/min/kg) (0.0059±0.0015) compared to control ( , and , n=4-11). The podocin relative expression was lower in MCYST35 (0.25 ± 0.05) compared to control ( , n=3). The right kidney, liver and lung index was higher in MCYST35 (0.0068± , 0.055± and ) compared to control ( , and , n=6). In this work it was observed that acute microcistin-lr intoxication induce changes in renal function and organs index EVALUATION OF REPRODUCTIVE BEHAVIOR AND GROWTH OF RODENT CERRADOMYS GOYTACA CAROLINA AUGUSTA BARROS; RODRIGO NUNES DA FONSECA; MARCOS FELIPE MARTINS GOMES; JOSUE MARCELO ALMEIDA; JACKSON DE SOUZA MENEZES UFRJ, MACAÉ, RJ, BRASIL. Cerradomys goytaca is the lasted mammal discovered in the Sandbank Jurubatiba National Park (North of Rio de Janeiro State). The reproduction of this rodent in vivarium is the initial steps to evaluate physiological adaptations to survive in sandbank environment. The animals were fed with regular rat/mouse chow and water ad libitum. They were kept in daily 12-hour cycle of light/dark and temperature of 23 ± 1 C and relative humidity of 60%. The offspring were monitored weekly from seventh to twenty first week after delivery by measuring the weight (g), naso-anal and caudal length (cm). The gestational age was 28 to 32 weeks and weaning occurred in the sixth week after delivery. The mean number of offspring was 4.3±0.3. The rate of newborn mortality was about 20%. One week after weaning, the weight (g) of females was 42.0±2.7 and males was 34.0±1.0. The males gain of weight (g) were 41.0±6.0 and females were 38.6±3,1 (p>0.05) in 14 weeks after weaning. The body males longitudinal growth and caudal growth (cm in 14 weeks after weaning) were respectively 7.35±1.11, 3.55±0.45 and females were, respectively 6.38 ± 0.72, 4.18±0.52 (p>0.05). It was not observed a significant correlation between room humidity / pregnancy and female age / maternal cannibalism. It was observed a moderate negative correlation between room temperature and pregnancy. Cerradomys goytaca rodents are able to reproduce and survive in animal vivarium EVALUATION OF THE H2O TRANSPORT MEDIATED BY HUMAN UREA TRANSPORTERS A1, A2, A3 AND B HETEROLOGOUSLY EXPRESSED IN RANA CATESBEIANA OOCYTES JESSICA KABUTOMORI UNIVERSITY OF SAO PAULO, SAO PAULO, SP, BRASIL. Urea contributes to the inner medullary osmolality gradient, important for regulating H2O excretion under the influence of antidiuretic hormone. Mammalian urea transporters (UTs,SLC14 family) facilitate diffusion of urea across the plasma membrane:uta1 is expressed on the apical membrane and UTA3 on the basolateral membrane in the inner medulla, UTA2 in the thin descending limb in the outer and inner medulla, and UTB in the descending vasa recta. Although it is clear that human (h) UTs plays a critical role in the urinary concentrating mechanisms, there is some debate as to whether huts also facilitate H2O transport in the kidney. Previously, it was shown that hut-b can function as a H2O channel. This study explores the osmotic water permeability (Pf) of huta1, A2, A3, and B heterologously expressed in Rana catesbeiana oocytes using video microscopy to monitor cell swelling as we reduce the extracellular osmolality. Before functionally characterizing the huts, we performed western blotting experiments of biotinylated samples to confirm that the UTs were inserted into the plasma membrane. Our Pf values for UTA2 ( /-0.001,n=18), UTA3 ( / ,n=15) and UTB ( / ,n=8) expressing oocytes were all significantly greater than that of control H2O-injected ( / ,n=24) oocytes, while UTA1 expression had no effect on the oocyte Pf. Thus, our data confirm that UTB conducts H2O, similar to the observation seen by others using Xenopus oocytes, and indicate for the first time that UTA2 and UTA3-expressing Rana oocytes have significant H2O permeability EFFECT OF PHYSICAL TRAINING IN THE EVOLUTION OF ACUTE RENAL INJURY IN RATS: ROLE OF ENDOTHELIAL CELLS HELOISA DELLA COLETTA FRANCESCATO; CAMILA DE MATTOS FALEIROS; MARCELO PAPOTI; LUCAS CHAVES; ROBERTO SILVA COSTA; TEREZILA MACHADO COIMBRA UNIVERSITY OF SAO PAULO, RIBEIRAO PRETO, SP, BRASIL. Endothelial dysfunction occurs in acute renal failure, with increased expression of antiangiogenic factors and loss of angiogenic factors such as vascular endothelial growth factor (VEGF), contributing to the establishment and progression of renal injury. This study evaluated the effect of physical training in acute kidney injury (administration of cisplatin, CP) on the regeneration of the endothelium as well in the inflammatory process. Male Wistar rats (n=12) previously submitted or not to physical training in a treadmill running (4 weeks) were injected with CP (5 mg/kg) (PhT+CP and CP groups, respectively) or saline (Control group). Blood and urine samples were collected 5 days after injections to evaluate plasma creatinine levels (Pcreat;mg%) and sodium fractional excretion (FENa+;%), and kidneys were removed for immunohistochemical studies (macrophages and VEGF expressions). CP-treated rats presented increased Pcreat, FENa+, and number of macrophages (ED1+ cells/100mm2) in the renal outer medulla, which were attenuated in PhT+CP group. There was a greater immunostaining (evaluated by score) for VEGF in renal outer medulla in PhT+CP group compared to C and CP groups. We found a negative correlation between macrophage infiltration and VEGF expression (R=0.786;p=0.0015). The effect of physical training in renal lesion provoked by CP was associated with less inflammatory process and increased VEGF expression, contributing to decrease of renal damage. THEME 03 NEUROPHYSIOLOGY MUSCIMOL INJECTED INTO THE LATERAL PARABRACHIAL NUCLEUS INCREASES POTASSIUM CHLORIDE INTAKE IN THE RAT 4

5 JOAO CARLOS CALLERA 1 ; LAURIVAL ANTONIO DE LUCA JR 2 ; JOSÉ VANDERLEI MENANI 2 1.DEPT. OF BASIC SCIENCES, SCHOOL OF DENTISTRY, UNIVERSIDADE ESTADUAL PAULISTA (UNESP), ARACATUBA, SP, BRASIL; 2.DEPT. OF PHYSIOLOGY AND PATHOLOGY, SCHOOL OF DENTISTRY, UNESP, ARARAQUARA, SP, BRASIL. Muscimol, GABAA receptor agonist, into the lateral parabrachial nucleus (LPBN) results in ingestion of water and different mineral solutions containing sodium (0.3 M NaCl or 0.3 M NaHCO3). In the present study, we investigated if muscimol into the LPBN would modify the ingestion of other mineral solution (potassium chloride, KCl) in normohydrated, in cell-dehydrated rats by an intragastric load of NaCl (IG 2 M NaCl) or in rats with sodium depletion (treatment with the diuretic furosemide subcutaneously + sodium deficient food for 24 h). Deionized water and 0.3 M KCl intake was measured at every 30 min during 240 min, starting 15 min after injections of muscimol (0.5 nmol/0.2 ml) or saline into the LPBN. Muscimol into the LPBN increased 0.3 M KCl intake in normohydrated rats (5.1 ± 1.4, vs. saline: 0.1 ± 0.1 ml/240 min), in rats treated with IG 2 M NaCl (20.6 ± 2.7, vs. saline: 1.0 ± 0.6 ml/240 min) and in sodium-depleted rats (8.3 ± 2.9, vs. saline: 0.5 ± 0.1 ml/240 min). Muscimol into the LPBN also increased water intake in normohydrated rats and in sodium-depleted rats, however, muscimol reduced water intake in the first 150 min of test in rats treated with IG 2 M NaCl. These data show that besides sodium and water intake, muscimol also increase 0.3 M KCl intake in normohydrated, cell-dehydrated and sodium depleted rats, suggesting that the activation of GABAA receptors in the LPBN releases the ingestion of different mineral solutions REABILITAÇÃO NEUROMUSCULAR ATRAVÉS DE MUSICOTERAPIA NATHALYA DE CARVALHO AVELINO 1 ; MARLY CHAGAS OLIVEIRA PINTO 2 ; OVIDIU CONSTANTIN BALTATU 1 ; LUCIANA APARECIDA CAMPOS 1 1.CAMILO CASTELO BRANCO UNIVERSITY (UNICASTELO), SAO JOSE DOS CAMPOS, SP, BRASIL; 2.2CONSERVATÓRIO BRASILEIRO DE MÚSICA - CENTRO UNIVERSITÁRIO, RIO DE JANEIRO, RJ, BRASIL. Introdução: Reabilitação neuromuscular com exercícios específicos com musicoterapia induzem treinamento de feedback multissensorial através da ativação do acoplamento auditório sensório motor. O objetivo deste estudo foi investigar os efeitos da musicoterapia na reabilitação neuromuscular de pacientes com trauma musculoesquelético no pós-operatório. Material e Métodos: Pacientes pós-operatório foram divididos em um grupo controle apenas com fisioterapia e um grupo submetido a fisioterapia e musicoterapia combinados com exercícios específicos. Força, amplitude de movimento de extensão e flexão de punho foram avaliadas por dinamômetro Kratos, e movimento angular por goniômetro durante 5 dias de reabilitação. Resultados: A força aumentou significativamente entre os dias 1 e 5 (13.0 ± 2.6 vs 23.0 ± 2.6 kgf, respectivamente) e dias 2 e 5 (16.0 ± 3.1 vs 23.0 ± 2.6 kgf, respectivamente) somente no grupo com musicoterapia. A amplitude de flexão aumentou significativamente a partir do segundo dia de musicoterapia (41.0 ± 5.0), enquanto apenas no dia 5 (34.5 ± 8.2) no grupo controle. No final da reabilitação o grupo com musicoterapia havia superado o grupo controle quanto a amplitude de flexão (51 ± 9.2 vs 34.5 ± 8.2). A amplitude de extensão aumentou de forma significativa a partir do quarto dia de musicoterapia (42.0 ± 7.8), enquanto que no grupo controle este aumento ocorreu apenas no quinto dia (64.5 ± 7.8). Conclusoes: O treinamento motor com suporte da música pode representar uma estratégia terapêutica eficaz para a reabilitação neuromuscular pós-operatória TREATMENT WITH L-DOPA DOES NOT RESTORE RESPIRATORY IMPAIRMENT IN AN EXPERIMENTAL MODEL OF PARKINSON'S DISEASE ARIADINY DE LIMA CAETANO; MARINA TUPPY; THIAGO S MOREIRA; ANA CAROLINA T TAKAKURA UNIVERSIDADE DE SAO PAULO, SAO PAULO, SP, BRASIL. Parkinson's disease (PD) is a neurodegenerative disease characterized by the loss of dopaminergic neurons in the substantia nigra (SN). The symptoms are: tremor at rest, muscular rigidity, bradykinesia, hypokinesia and postural disturbance. Aside from the classic symptoms, there are secondary symptoms associated with PD, like respiratory deficiencies. The classic pharmacological treatment for PD is the use of L-3,4-dihydroxyphenylalanine (L-DOPA). Recently our laboratory showed a respiratory failure in a 6-hydroxydopamine (6-OHDA) animal model of PD. The aim of this study was to verify whether L-DOPA treatment would be able to restore breathing in our PD model. Male Wistar rats ( g, N=5) received bilateral 6-OHDA (12µg/µL) injection in the striatum. Twenty days after 6-OHDA injection, treatment of saline or L-DOPA (Prolopa HBS, 100mg/benserazide hydrochloride 25mg, ip) were performed for 40 days. The functional respiratory responses were measured by whole-body plethysmography and immunohistochemistry was performed to evaluate the SN lesion. Bilateral injection of 6-OHDA in the striatum elicited reduction of 80% of catecholaminergic neurons in the SN. The L-DOPA treatment did not restore the reduction in respiratory frequency (88±11; vs. saline: 92±11bpm) produced by 6-OHDA in the striatum. This study shows that treatment with L- DOPA is not able to restore the breathing deficits observed in the PD model induced by bilateral injection of 6-OHDA in the striatum MINERAL PREFERENCE IN RATS TREATED WITH INJECTIONS OF MUSCIMOL INTO THE LATERAL PARABRACHIAL NUCLEUS JOAO CARLOS CALLERA 1 ; LAURIVAL ANTONIO DE LUCA JR 2 ; JOSÉ VANDERLEI MENANI 2 1.DEPT. OF BASIC SCIENCES, SCHOOL OF DENTISTRY, UNIVERSIDADE ESTADUAL PAULISTA (UNESP), ARACATUBA, SP, BRASIL; 2.DEPT, OF PHYSIOLOGY AND PATHOLOGY, SCHOOL OF DENTISTRY, UNESP, ARARAQUARA, SP, BRASIL. It was previously demonstrated that rats treated with bilateral injections of muscimol, GABAA receptor agonist, into the lateral parabrachial nucleus (LPBN) ingest water and different mineral solutions containing sodium (0.3 M NaCl or 0.3 M NaHCO3) in a two-bottle choice test. In the present study, we investigated mineral preference after bilateral injections of muscimol into the LPBN in normohydrated rats or in hyperosmotic cell-dehydrated rats treated with intragastric (IG) load of 2 M NaCl. Male adult Wistar rats (n=6) with bilateral stainless steel guide-cannulas implanted into the LPBN had deionized water and four mineral solutions at 0.3 M concentration (NaCl, NaHCO3, CaCl2 and KCl) simultaneously available for consumption in a five-bottle test. The ingestion was measured at every 30 min during 240 min, starting 15 min after the injections into the LPBN. Normohydrated rats treated with bilateral injections of muscimol (0.5 nmol/0.2 µl) into the LPBN ingested mainly 0.3 M NaCl and a less amount of NaHCO3, but not CaCl2, KCl or water. Cell-dehydrated rats treated with IG 2 M NaCl combined with injections of muscimol into the LPBN ingested more 0.3 M NaCl than all other fluids. These data suggest a clear preference for sodium intake when the inhibitory mechanisms are blocked by GABAA receptor activation with muscimol injections into the LPBN in rats A6 NORADRENERGIC NEURONS ARE RECRUITED IN A RAT MODEL OF PARKINSON S DISEASE LUIZ MARCELO OLIVEIRA; MARINA TUPPY; THIAGO S MOREIRA; ANA CAROLINA T TAKAKURA UNIVERSITY OF SÃO PAULO, SAO PAULO, SP, BRASIL. Previous study has already demonstrated that in 6-hydroxydopamine (6-OHDA)-model of Parkinson s disease (PD) there is a reduction in the number of Phox2b neurons in the retrotrapezoid nucleus (RTN) and a decrease in the respiratory response to hypercapnia 40 days after PD-induced. This functional deficiency is restored 60 days after 6-OHDA injection and here we tested the hypothesis that A6 noradrenergic cells could be a candidate to restore this deficiency in this model. Minute Ventilation (VE) in response to hypercapnia (7% CO2) was assessed one day before and 60 days after bilateral 6-OHDA (24 µg/µl) or vehicle injections in the striatum (CPu) and in the A6 in Male Wistar rats (CEUA: 14, p.15, b.03/2014). Bilateral injections of 6- OHDA decreased cathecolaminergic neurons by 86% and 83% in the substancia nigra (SN) and A6, respectively. In animals with lesion in the SN and A6 (N = 5/group) there is a reduction in the ventilatory response to hypercapnia (785±18 vs vehicle: 1417±177 ml/kg/min). In another group of rats after 60 days of injections of 6-OHDA in the CPu (N = 4/ group), the rats were exposed to hypercapnia or normocapnia for 3 hours and there was a reduction in the number of hypercapniainduced-fos-ir cells in the RTN region (8.5 ±0.9, vs. vehicle 78±3). However, in PD rats, hypercapnia was able to induce Fos-ir cells in the A6 region (94±22 vs. vehicle 1±1). Our data suggest that A6 noradrenergic neurons can be a candidate to assume the chemoreceptor function in a model of PD EFFECTS OF CHRONIC UNPREDICTABLE STRESS ON DEPRESSIVE BEHAVIOR IN RATS WITH PREMATURE OVARIAN FAILURE JORDANA LAIS DE ROCCO 1 ; FLAVIANO LORENZON 1 ; TAMIRES GREGORIO 1 ; BRUNA BARCELOS DE SIMAS 1 ; JANETE APARECIDA ANSELMO-FRANCI 2 ; CILENE LINO OLIVEIRA 1 ; DOMITILA AUGUSTA HUBER 1 ; FERNANDA BARBOSA LIMA CHRISTIAN 1 1.UNIVERSIDADE FEDERAL DE SANTA CATARINA, FLORIANOPOLIS, SC, BRASIL; 2.UNIVERSIDADE DE SAO PAULO, RIBEIRA PRETO, SP, BRASIL. Stress can contribute to the higher incidence of affective disorders in women during perimenopause. We have investigated the impact of chronic unpredictable stress (CUS) on behavior in the forced swim test (FST) in rats with premature ovarian failure (POF). Female rats (28 days old) were treated with 4-vinylcyclohexane diepoxide (VCD 160 mg/kg, ip) or vehicle (CTL) for 15 consecutive days. After 70 days, rats were submitted to CUS for 10 days, including 4 sections of FST. Proactive (swimming, diving and climbing) and passive (immobility) behaviors were evaluated. VCD group increased immobility (Day 2: 98±62; Day 4: 144±76) and decreased climbing (Day 2: 169±65; Day 4: 115±66) on the last day of FST. CTL rats increased duration (Day 1: 20±16; Day 3: 54±25) and frequency (Day 1: 2.5±1.3; Day 3: 8.5±3) of swimming on day 3 of FST. VCD group presented higher latency for swimming (Day 1: 54±67; Day 2: 149±92) and diving (Day 1: 94±117; Day 4: 274±76) in day 4 of FST. Both duration (Day 4 VCD: 0.43±14; Day 4 CTL: 7.4±8,) and frequency (Day 4 VCD: 0.22±0.7; Day 4 CTL: 3.8±4) of diving were significantly lower in VCD rats compared to CTL. Latency to dive was higher in VCD compared to CTL (Day 4 VCD: 274±76 sec; Day 4 CTL: 142±101sec). The results indicate that POF leads rats under chronic unpredictable stress to develop passive strategies to cope with stress in the FST. Hence, during the progression of POF, stress may reinforce depressive disorders WATER DEPRIVATION-INDUCED SODIUM APPETITE IS ENHANCED IN RENOVASCULAR HYPERTENSIVE RATS CAMILA FERREIRA RONCARI; RAFAELA MOREIRA BARBOSA; LAURIVAL ANTONIO DE LUCA JR; JOSÉ VANDERLEI MENANI; EDUARDO COLOMBARI; DEBORA SIMÕES ALMEIDA COLOMBARI DEPARTMENT OF PHYSIOLOGY AND PATHOLOGY, DENTISTRY SCHOOL, UNESP, ARARAQUARA, SP, BRASIL. Sodium appetite dependent on the activation of the renin angiotensin system (RAS) is induced after water deprivation (WD) followed by water intake (partial rehydration - PR). Rats with 2- kidney, 1-clip (2K1C) hypertension present an overactive RAS. In the present study, we evaluated 0.3 M NaCl and water ingestion in normotensive and 2K1C hypertensive rats submitted to the WD- PR protocol. Male Holtzman rats ( g, n = 5/group) received a silver clip around the left renal artery (2K1C hypertensive rats) or no clip around the artery (sham rats). After six weeks, 2K1C hypertensive and sham rats were submitted to 24 h of WD, before the access to only water to ingest for 2 h (PR). At the end of this period, 0.3 M NaCl was also available and the intake of both 0.3 M NaCl and water was recorded for an additional 2 h (salt appetite test). After WD, 2K1C hypertensive and sham rats ingested similar amount of water intake during the PR (15.9 ± 4.1, vs. sham: 14.8 ± 1.2 ml/2 h). Compared to sham rats, in the salt appetite test, 2K1C hypertensive rats ingested more 0.3 M NaCl (8.0 ± 1.2, vs. sham: 3.4 ± 1.2 ml/2 h) and water (10.3 ± 4.4, vs. sham: 2.3 ± 0.9 ml/2 h). The results show that 2K1C hypertensive rats have a stronger sodium appetite than normotensive rats when submitted to the WD-PR protocol, probably due to the overactivity of RAS in these animals INIBIÇÃO DA ISOFORMA NEURONAL DA NOS NÃO ALTERA HORMÕNIOS NEUROHIPOFISÁRIOS DE RATOS SUBMETIDOS A SOBRECARGA SALINA FABIANA LUCIO-OLIVEIRA 1 ; JULIANA BEZERRA MEDEIROS DE LIMA 2 ; FERNANDA MARIA VEANHOLI VECHIATO 2 ; RICARDO COLETTI 2 ; TATIANE VILHENA FRANCO 2 ; LUCILA LEICO KAGOHARA ELIAS 2 ; JOSÉ ANTUNES-RODRIGUES 2 1.INSTITUTO FEDERAL DO SUL DE MINAS GERAIS, MUZAMBINHO, MG, BRASIL; 2.FMRP, RIBEIRAO PRETO, SP, BRASIL. O óxido nítrico tem sido sugerido exercer um papel proeminente na regulação do eixo hipotálamohipófise. O objetivo deste estudo foi avaliar o efeito do N-propil-L- arginina (NPLA), um inibidor seletivo da NOS neuronal, sobre os níveis plasmáticos de AVP e OT em ratos submetidos à sobrecarga de sal. Ratos wistar machos implantados ICV permaneceram em SS por 96 horas. Uma hora e 30 minutos antes de decapitação, os ratos foram tratados com uma injecção ICV de NPLA ou veículo. Uma hora após a injeção icv, água e solução NaCl 0,3 M foram novamente oferecida. Após esse período, os animais foram decapitados, seu sangue foi coletado para determinação da osmolalidade e para dosagens hormonais. O cérebro foi removido e o HMB foi coletado para a dosagem da atividade da NOS. Os nossos dados mostraram, o aumento da osmolalidade de ratos que foram submetidas a SS. O aumento da osmolalidade foi seguido por um aumento na atividade NOS no HMB de ratos com SS. Entretanto, os animais submetidos a SS tratados com NPLA tiveram uma diminuição na atividade NOS cerca de 44% quando comparado aos animais tratados com veículo. Houve um aumento significativo de AVP e OT em animais submetidos à SS, mas nenhuma diferença foi observada no tratamento com NPLA. Estes resultados indicam que a modulação de NO sobre a secreção de AVP e OT observado em vários estudos provavelmente não é realizada através da isoforma neuronal de NOS, ou que o nível de inibição de NOS não foi eficaz na modulação da resposta desses hormônios durante estímulos osmóticos como SS HYPOXIA ACTIVATES BRAINSTEM AND HYPOTHALAMIC NEURONS THAT EXPRESS LEPTIN RECEPTORS MIRIAN BASSI 1 ; JOSÉ VANDERLEI MENANI 1 ; DEBORA SIMÕES ALMEIDA COLOMBARI 1 ; DANIEL BRESEGHELLO ZOCCAL 1 ; JOSE DONATO JR 2 ; EDUARDO COLOMBARI 1 1.UNESP, ARARAQUARA, SP, BRASIL; 2.USP, SAO PAULO, SP, BRASIL. Leptin facilitates chemoreflex ventilatory responses by mechanisms not completely clear yet. Therefore, in this study, we evaluated c-fos expression induced by hypoxia in brainstem and hypothalamic neurons that express leptin receptors (LRs) to identify possible sites of leptin action to modulate breathing. Male LepR-reporter mouse (n=5) that allow to visualize LR-expressing cells with the tdtomato fluorescent protein were used. Cells with c-fos expression were identified using immunohistochemistry procedures after the exposure of mice to hypoxia (7% of O2 in breathing air) during 90 min followed by additional 90 min in normoxic air. The LRs and c-fos expression was quantified in one coronal section (30 µm) of each analyzed nucleus. LRs were predominant, among other areas, in the commissural nucleus of the solitary tract (cnts: 50±5 cells/section), lateral parabrachial nucleus (LPBN: 119±6 cells/section) and arcuate nucleus of hypothalamus (ARC: 139±20 cells/section). Hypoxia induced c-fos expression co-localized with LRs mostly in the cnts (49%) and ARC cells (85%). The LPBN showed low percentage of cells coexpressing c-fos/lrs (4%). The locus coeruleus, an important area for the ventilatory regulation, is also activated by hypoxia (c-fos: 63±22 cells/section), however, no labelling for LRs were detected in this area. These data show that cnts and ARC are main areas with LRs cells activated by hypoxia and, therefore, possible sites of action of leptin to modulate the ventilatory responses to hypoxia INTERNAL BODY TEMPERATURE SIGNALS TO BIOLOGICAL CLOCKS THROUGH TRPV1 CHANNELS NAYARA ABREU COELHO HORTA 1 ; FREDERICO SANDER MANSUR MACHADO 1 ; THAIS SANTANA ROCHA CARDOSO 1 ; MAURO SÉRGIO BATISTA SILVA 1 ; CÂNDIDO CELSO COIMBRA 1 ; SAMUEL PENNA WANNER 1 ; ANA MARIA DE LAURO CASTRUCCI 2 ; MARISTELA DE OLIVEIRA POLETINI 1 1.FEDERAL UNIVERSITY OF MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSITY OF SÃO PAULO, SÃO PAULO, SP, BRASIL. Our group has been studying the role of TRPV1 channels in signaling temperature to peripheral clocks. In a previous study, we observed that acute blockade of TRPV1 channels alters the expression of clock genes in peripheral tissues. In this study, we investigated whether desensitization TRPV1 disrupts the molecular clock oscillation in peripheral tissues. Rats received an injection of 20 µg/kg body weight ip of resiniferatoxin (RTX) and after five days were euthanized at zeitgeber (ZT) 0 (time of light on), ZT6, ZT12 and ZT18. The total RNA was extracted 5

6 from the liver, adrenal and microdissections of the suprachiasmatic nucleus (SCN). Relative analyses of clock genes, rper1 and rbmal1 mrna, were performed by qpcr. The desensitization abolished the adrenal circadian profiles of rper1 and rbmal1 expression. This was also observed in liver only for rper1 expression, although rbmal1 was increased at ZT0. Intriguingly, in the SCN (the central clock), TRPV1 desensitization altered the circadian profile of rbmal1 expression regardless of changes on rper1 circadian expression. We previously observed that acute blockade of TRPV1 channels decreases corticosterone plasma levels, this may be a secondary signal from peripheral clocks to the central pacemaker, since RTX induced desensitization is restricted to the abdominal cavity. Thus, TRPV1 may mediate the temperature-induced rhythm of peripheral clocks ROLE OF NITRIC OXIDE OF THE DORSOLATERAL PERIAQUEDUCTAL GRAY MATTER IN RESTRAINT-INDUCED FEVER IN RATS VICTOR MARTINS; CAROLINE CRISTINA SILVA; LUCIANE HELENA GARGAGLIONI BATALHAO; KÊNIA CARDOSO BÍCEGO COLLEGE OF AGRICULTURAL AND VETERINARIAN SCIENCES - SAO PAULO STATE UNIVERSITY, JABOTICABAL, SP, BRASIL. Nitric oxide (NO) acts in different brain regions as a pyrogenic or antipyretic agent during systemic inflammation induced by endotoxin. There is evidence of its role in restraint-induced fever but it is unknown its site of action in the brain. The periaqueductal gray (PAG) is a possible site of action for NO because it is involved in thermoregulation and stress responses (fear and defensive behavior). The dorsolateral PAG (PAGdl) expresses large amount of the enzyme NO synthase (NOS). Thus, the aim of this study was to test the hypothesis that NO acts in the PAGdl as an antipyretic agent in restraint stress-induced fever in rats. To this end, it was verified the effect of microinjection of non-selective NOS inhibitor, L-NMMA, in PAGdl on body temperature (Tb) and on heat loss index (HLI) of Wistar rats exposed or not to physical restraint. No difference was observed in Tc of control rats (saline: /- 0.1; L-NMMA: /- 0.1). During physical restraint, there was an increase in Tb of all groups, but the response was more pronounced after L-NMMA injection in PAGdl. The HLI similarly increased in all groups after restraint stress. These preliminary results indicate that the NO seems to act as antipyretic molecule in PAGdl during fever induced by restraint stress, without affecting the heat dissipation mechanism ROLE OF MU AND KAPPA OPIOID RECEPTORS OF THE PERIAQUEDUCTAL GRAY IN RESTRAINT-INDUCED FEVER CAROLINE CRISTINA SILVA; VICTOR MARTINS; LUCIANE HELENA GARGAGLIONI BATALHAO; KÊNIA CARDOSO BÍCEGO COLLEGE OF AGRICULTURAL AND VETERINARIAN SCIENCES, SÃO PAULO STATE UNIVERSITY, JABOTICABAL, SP, BRASIL. The endogenous opioids are involved in analgesia, thermoregulation and physiological responses to various stressful stimuli such as infection, psychological stress and hypoxia. The mu and kappa receptors in the hypothalamus play a role in endotoxin-induced fever and hypoxia-induced anapyrexia (opposite response to fever), respectively. In addition, periaqueductal gray (PAG), which express both mu and kappa receptors, is involved in defense and thermoregulatory responses. Thus, our hypothesis is that mu and kappa opioid receptors in the PAG modulate the restraint-induced fever in rats by activating and inhibiting this response, respectively. To test this hypothesis, it was verified the effect of intra-pag injection of mu antagonist (CTAP) and kappa antagonist (nor-bni) on body temperature (Tb; datalogger implanted in the abdominal cavity) of Wistar male rats submitted or not to restraint stress. The Tc of control animals did not differ between saline (37.35 ± 0.097), nor-bni (37.75 ± 0.148) and CTAP (37.35 ± 0.092) groups. Restraint stress increased the Tb of nor-bni and saline groups similarly, but the increase in Tb was smaller in those animals treated with CTAP. The results indicate that mu, but not kappa, opioid receptors in the PAG seem to act as pyrogenic agents during fever induced by restraint stress in rats INTERLEUKIN-1 RECEPTOR ANTAGONIST DECREASES HYPOTHALAMUS OXIDATIVE STRESS DURING EXPERIMENTAL SEPSIS CARLOS HENRIQUE ROCHA CATALÃO; FAZAL WAHAB; RODRIGO PEREIRA DE ALMEIDA RODRIGUES; NILTON NASCIMENTO SANTOS-JÚNIOR; LUIS HENRIQUE ANGENENDT DA COSTA; MARIA JOSÉ ALVES ROCHA UNIVERSIDADE DE SÃO PAULO, RIBEIRAO PRETO, SP, BRASIL. Purpose: We investigated the effect of IL-1ra pre-treatment on the sepsis-induced increase in oxidative stress markers in the hypothalamus of rats. Methods: The animals were pre-treated by an icv injection of IL-1ra (9-nmol) or vehicle (0.01M PBS) before being subjected to cecal ligation and puncture (CLP), or left as control (shamoperation or naive). After 4, 6 and 24h, the animals were decapitated (n=8/group) and the brain removed for hypothalamic tissue collection. Transcript and protein levels of IL-1, inducible nitric oxide synthase (inos), caspase-3 and hypoxia-inducible factor 1-alpha (HIF-1α) were quantified by quantitative polymerase chain reaction (qpcr) and western blot, respectively. Results: Hypothalamic mrna levels of all these genes were significantly (p<0.005) increased at 4, 6 and 24h CLP, as compared to sham operated animals. IL-1ra pre-treatment in these CLP animals significantly decreased IL-1 gene expression at all-time points and also of inos, caspase-3 and HIF-1α at 24h when compared to vehicle treated CLP animals. The effect of the pre-treatment on protein expression was most clearly seen for IL-1β and inos at 24h. Conclusion: Our results showed that blocking the IL-1-IL-1r signaling pathway by central administration of an IL-1ra decreases hypothalamic oxidative stress markers during sepsis ROLE OF COMMISSURAL NUCLEUS OF SOLITARY TRACT ON MAINTENANCE OF HYPERTENSION AND ON RESPIRATORY ACTIVITY IN 2K1C RATS MARIANA DEL ROSSO MELO; SILVIA GASPARINI; GUILHERME F.F. SPERETTA; JOSÉ VANDERLEI MENANI; DANIEL BRESEGHELLO ZOCCAL; DEBORA SIMÕES ALMEIDA COLOMBARI; EDUARDO COLOMBARI ADEPARTMENT OF PHYSIOLOGY AND PATHOLOGY, UNESP, ARARAQUARA, SP, BRASIL. We have demonstrated that 2K1C hypertensive rats present baseline higher ventilation and increased ventilatory response to hypoxia, which suggests peripheral chemoreflex overactivity. In the present we aimed to evaluate the role of comissural nucleus of solitary tract (cnts) on the maintenance of hypertension and on diaphragm activity in 2K1C rats. Male Holtzman rats ( g) received a silver clip around the left renal artery to induce renovascular hypertension (n=6). Rats with sham surgery were used as normotensive control (NT, n = 7). Six weeks after the surgery, saline (NaCl 0.15 M, 60 nl) followed 20 min later by isoguvacine (20 mm; 60 nl gabaergic agonist) were microinjected into the cnts of urethane (1.4 g/kg of body weight; i.v.) anesthetized rats and mean arterial pressure (MAP) and diaphragm activity (DA) were recorded for an additional 60 min. Baseline MAP in 2K1C rats was higher than in NT rats (MAP: 193 ± 9, vs. NT: 123 ± 2 mmhg, p<0.05). Twenty min after isoguvacine injection, there was a greater decrease in MAP in 2K1C compared to NT rats (-40 ± 6, vs. NT: -16 ± 3 mmhg, p<0.05). DA also decreased after isoguvacine injection in 2K1C animals (Δ -19 ± 6 vs. NT: 1 ± 7 %, 60 min; p<0.05). These data showed that cnts is an important source to the maintenance of renovascular hypertension and diaphragm activity in 2K1C rats NEURONS FROM PAG RECEIVE PROJECTIONS FROM SN AND PROJECT TO RTN JULIANA CRISTINA DE LIMA; ANA CAROLINA T TAKAKURA; THIAGO S MOREIRA; MARINA TARANTO BOTELHO UNIVERSIDADE DE SAO PAULO, SAO PAULO, SP, BRASIL. Parkinson's disease (PD) is characterized by a reduction in the number of dopaminergic neurons of the substantia nigra (SN) accompanied by motor and non-motor deficiencies such as respiratory failure. Previous study showed that in a model of PD there is a decrease in respiratory rate and in the number of retrotrapezoid nucleus (RTN) neurons. Preliminary study from our laboratory has also shown that there are no direct projections from SN to RTN. Therefore, our objective was to investigate if the pathway between SN and RTN involves a relay in the midbrain periaqueductal gray (PAG), a region that plays an important role in physiological responses including breathing. Adulto male Wistar rats (N=4/group, CEUA: 99, page22, book 03) received unilateral injection of the anterograde tracer biotin-dextran-amide (BDA-10%) in the SN or PAG and retrograde tracer Fluorogold (FG-2%) in the RTN and PAG. The results showed the presence of varicosities containing BDA in the PAG (from BDA injection into the SN) and into the RTN (from BDA injection into the PAG). The results from retrograde tracer showed that there are FG labeled neurons in the PAG (from FG injection into the RTN) and FG+/TH+ labeled neurons in the SN (from FG injection into the PAG). In conclusion, our results showed that there is a direct projection from SN to PAG and from PAG to RTN, which suggest that the PAG is a relay between SN and the chemoreceptors neurons into the RTN NEURÕNIOS KNDY SÃO ATIVADOS PELO ESTRADIOL DURANTE OS PICOS PRÉ- OVULATÓRIOS DE HORMÕNIO LUTEINIZANTE E PROLACTINA EM FÊMEAS ROBERTA ARAUJO LOPES 1 ; RAQUEL GIL DE LIMA BERNARDES 1 ; FERNANDA LUIZA MENEZES BELLO 1 ; PATRÍCIA COSTA HENRIQUES 1 ; NAYARA SOARES SENA AQUINO 1 ; CELSO RODRIGUES FRANCI 2 ; RAPHAEL ESCORSIM SZAWKA 1 1.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSIDADE DE SÃO PAULO, RIBEIRÃO PRETO, SP, BRASIL. A kisspeptina (Kp) é essencial para secreção de hormônio luteinizante (LH) e fertilidade. Os neurônios KNDy do núcleo arqueado (ARC) co-expressam kisspeptina, neurocinina e dinorfina, e o seu papel no controle de secreção hormonal permanece obscuro. Utilizamos dupla marcação imunohistoquímica para investigar a atividade dos neurônios KNDy associada a mudanças na secreção de LH e prolactina (PRL) em ratos machos e fêmeas. Ratas ciclando foram perfundidas nas fases de diestro (n=6), 12 e 18 h do proestro (n=5-6) e estro (n=6). O número de neurônios Kp imunorreativos (Kp-ir) no ARC foi menor no proestro e no estro em relação ao diestro (P<0.01). Por sua vez, a porcentagem de neurônios Kp-ir com co-expressão de c-fos foi 45% maior às 18 h do proestro (P< 0.05). Em machos (n= 6-8), o número de neurônios Kp-ir no ARC foi menor em relação as fêmeas em diestro e a co-expressão de c-fos foi baixa e inalterada durante o dia (P= 0.68). Ratas ovariectomizadas (OVX) foram tratadas com óleo (OVX; n=8), estradiol (OVX+E; n=7) ou estradiol e progesterona (OVX+EP; n=6-7) e foram perfundidas às 12 e 18 h. O número de neurônios Kp-ir no ARC foi menor nos grupos OVX+E e OVX+EP em relação as ratas OVX (P<0.05).Por outro lado, a expressão de c- Fos nos neurônios KNDy aumentou em aproximadamente 50% às 12 e 18 h (P<0.05) em ratas OVX+E e somente às 12 h (P<0.01) em ratas OVX+EP.O estradiol reduz a expressão de Kp no ARC, porém, a população remanescente de neurônios KNDy permanece ativa no momento do pico de LH e PRL, o que demonstra um novo aspecto da regulação dos neurônios KNDy pelo estradiol CORRELATION BETWEEN NEUROANATOMICAL AND RESPIRATORY CHANGES IN AN EXPERIMENTAL MODEL OF PARKINSON'S DISEASE SILVIO DE ARAUJO FERNANDES-JUNIOR UNIVERSIDADE DE SÃO PAULO, SAO PAULO, SP, BRASIL. Parkinson's disease (PD) is a disorder characterized by motor and non-motor symptoms, such as respiratory deficits and is associated with loss of dopaminergic neurons in the substantia nigra (SN). Previous study showed that 40 days after the induction of an experimental model of PD in rats there is a decrease in the resting respiratory rate that lasted at least for 60 days. After 60 days, the immunohistochemical analysis showed a decrease in the number of Phox2b+-neurons of retrotrapezoid nucleus (RTN) and nucleus of the solitary tract (NTS). The aim of this study is to investigate if the neuroanatomical deficiency occurs before the functional respiratory abnormality observed. Adult male Wistar rats (n=13, CEUA 104/23/03) with bilateral injections of 6-hydroxydopamine (6-OHDA, 24 µg/µl) into the striatum were used. Respiratory parameters were evaluated one day before, 30 and 40 days after the injections. 6-OHDA produced 70% of reduction in catecholaminergic neurons of SN and reduced resting respiratory rate at 40 days after the lesion (79 ± 3 and 111 ± 19 vs. vehicle: 106 ± 5). Neuroanatomical results showed that 30 and 40 days after the lesion, the animals not presented a reduction of Phox2b+-neurons in the RTN (29±6 and 31±2, respectively vs. vehicle: 60±1) but yes in the NTS (387± 12 and 360±2, respectively vs. vehicle: 436±16). Our data suggest that neuroanatomical changes in NTS can be a cause for respiratory problems observed in PD. Key-words: Parkinson's disease, retrotrapezoid nucleus, respiratory changes NEONATAL OVERNUTRITION IMPAIRS HYPOTHALAMIC LEPTIN RESPONSIVENESS IN ADULTHOOD PAULA BEATRIZ MARANGON; BEATRIZ C. BORGES; JOSÉ ANTUNES-RODRIGUES; LUCILA LEICO KAGOHARA ELIAS FMRP - USP, RIBEIRAO PRETO, SP, BRASIL. Neonatal nutrition modifies circulating leptin levels, leading to changes in the maturation of hypothalamic circuitry and metabolic disturbances in adulthood. Using the manipulation of litter size as a model of nutritional programing, we aimed to investigate in male Wistar rats plasma leptin levels and the hypothalamic responsiveness to leptin. After birth, litters were adjusted as follows: small (3 pups-sl), normal (10 pups-nl) and large (16 pups-ll). Blood samples were collected at 8am on postnatal day 7 (P7), P10, P14, P21, P60 for Elisa assay. The following experiments were performed on P60. Animals were treated with leptin (500µg/Kg ip) or saline at 5pm. Food intake and body weight gain were evaluated 12h later; the BAT was collected for Western blotting 24h later. In another set of rats, 40min after leptin, animals were perfused and the brains collected for immunohistochemistry. A peak in plasma leptin on P10 was only observed in NL rats. SL rats had increased leptinemia in all days analyzed. After leptin, NL and LL rats reduced food intake and body weight gain, with no effect in SL rats. SL and LL rats had increased p-stat-3 expression in the ARC in vehicle treated animals. Leptin increased p-stat-3 expression in the ARC in NL rats and potentiated it in LL rats, with no effect in SL rats. Leptin increased UCP- 1 expression in the BAT in NL and LL rats. Our data indicate that neonatal changes in food availability modify hypothalamic leptin responsiveness in obese animals in adulthood EFFECTS OF HIGH SALT DIET DURING THE POSTNATAL PERIOD ON WATER AND SODIUM INTAKE INDUCED CELLULAR AND EXTRACELLULAR DEHYDRATION IN THE ADULT PHASE ARYANNE BATISTA SOARES DE MELO 1 ; THAIS MACHADO MERCÊS 1 ; GRAZIELA TORRES BLANCH 2 ; GUSTAVO RODRIGUES PEDRINO 1 ; ANDRE HENRIQUE FREIRIA-OLIVEIRA 1 1.UNIVERSIDADE FEDERAL DE GOIÁS, GOIANIA, GO, BRASIL; 2.PONTIFÍCIA UNIVERSIDADE CATÓLICA DE GOIÁS, GOIANIA, GO, BRASIL. Studies suggest that lived experiences in the initial stages of life, including the prenatal stage, are related to behavioral and pathophysiological changes in adulthood. This study investigated drinking and salt appetite induced by cellular and extracellular dehydration in rats submitted to high salt diet on postnatal age. Wistar male rats (21 days old) were kept with hypertonic saline solution (0.3 M NaCl, EXP) and food for 60 days. Control group was kept with water and food. Water and food was given for 15 more days (recovery). Subsequently, the animals received furosemide (10 mg, subcutaneously) and were kept in metabolic cages with access to water and poor sodium diet for 24 hours. After 24 hours, graduated burets with water and hypertonic saline (NaCl 0.3 M) were offered for 2 hours. EXP rats drank less water (EXP: 0.8 ± 0.5 vs. control: 1.5 ± 0.6 ml) and hypertonic saline (EXP: 4.5 ± 1.3 vs. control: 7.5 ± 2.6 ml) than control animals induced by extracellular dehydration. The cellular dehydration was induced by 2 M NaCl load (2 ml) gavage. The animals are maintained in metabolic cages, and one hour after the gavage, water was offered to animals for 2 hours. We did not observe any difference on water intake (EXP: 10.5 ± 1.6 vs. control: 9.9 ± 1.5 ml). The results suggest that the extracellular thirst and sodium appetite are affected by postnatal dehydration experiences NEURON-GLIA INTERACTION IN NUCLEUS TRACTUS SOLITARIUS IS REDUCED BY SHORT- TERM SUSTAINED HYPOXIA DANIELA ACCORSI-MENDONÇA; CARLOS EL ALMADO; LENI GH BONAGAMBA; JACI AIRTON CASTANIA; DAVI JA MORAES; BENEDITO HONORIO MACHADO UNIVERSITY OF SAO PAULO, ESTRANGEIRO, SP, BRASIL. Sustained hypoxia (SH) produces significant changes in physiological functions due to peripheral chemoreflex activation. We analyzed the effect of short-term SH (24 hours, FIO210%) on the processing of cardiovascular and respiratory reflexes using an in situ preparation of rats. SH increased both the sympatho-inhibitory and bradycardiac components of baroreflex as well as 6

7 sympathetic and respiratory responses to chemoreflex activation. We also analyzed the neurotransmission in nucleus tractus solitarius (NTS) neurons using brainstem slices and wholecell patch clamp. The 2nd-order NTS neurons were identified by previous application of tracer onto carotid body for or aortic depressor nerve. SH increased the frequency of action potential (AP) after positive injected current in NTS neurons. Delayed excitation, related to A-type potassium current (IKA) and sensitive to 4-aminopyridine, was observed in most of NTS neurons from control rats and IKA amplitude was higher in identified 2nd-order NTS neurons in control than in SH rats. SH also blunted the astrocytic inhibition of IKA in NTS neurons and increased the synaptic transmission in response to afferent fibers stimulation. The frequency of spontaneous excitatory currents was also increased in neurons from SH rats with no change in amplitude or half-width. Therefore, SH changed the glia-neuron interaction, increasing the intrinsic excitability and excitatory transmission of NTS neurons, which may contribute to the observed increase in the reflex sensitivity of baro- and chemoreflex in in situ preparation. THEME 04 CARDIOVASCULAR PHYSIOLOGY A INTENSIDADE DO EXERCÍCIO PROMOVE DIFERENTES ALTERAÇÔES NOS PARÂMETROS DE CONTRATILIDADE MIOCÁRDICA EM RATAS TREINADAS POR NATAÇÃO PAULA ANDRÉA MALVEIRA CAVALCANTE 1 ; MAURO SÉRGIO PERILHÃO 1 ; LEONARDO DOS SANTOS 2 ; ANDREY JORGE SERRA 3 ; PAULO JOSÉ FERREIRA TUCCI 4 ; DANILO SALES BOCALINI 1 1.UNIVERSIDADE SÃO JUDAS TADEU, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DO ESPÍRITO SANTO, VITÓRIA, ES, BRASIL; 3.UNIVERSIDADE NOVE DE JULHO, SÃO PAULO, SP, BRASIL; 4.UNIVERSIDADE FEDERAL DE SÃO PAULO, SÃO PAULO, SP, BRASIL. As adaptações miocárdicas relativas às diferentes intensidades de exercício ainda são controversas. Assim, o objetivo do estudo foi avaliar as adaptações da contratilidade miocárdica de ratas treinadas por natação com diferentes intensidades de exercício. Ratas Wistar foram distribuídas aleatoriamente em três grupos experimentais: Controle(C) e treinados (8sem,5 dias/sem,60 min/dia) moderadamente (Tm:sem peso adicional à cauda) e intensamente (Ti:com carga relativa a 8% do peso corpóreo adicionado). Foram determinadas a capacidade física (CF:seg), as tensões (g/mm2/mg) desenvolvida (TD) e repouso(tr), as derivadas (g/mm2/mg/s) positivas (+dt/dt) e negativas (-dt/dt). Após oito semanas de treinamento a CF (C:141±12;Tm:293±10;Ti:371±20;seg) diferiu entre os grupos. Os valores da TD(0,85±0,4), +dt/dt e -dt/dt(7±0,97 e 5±0,34) do grupo C foram inferiores aos grupos Tm(1,07±0,1;11±1,07 e -7±0,52) e Ti(1,27±0,7;15±0,59 e -10±1,06). A atividade do retículo sarcoplasmático do grupo Ti foi aprimorada em 52±3% diferindo dos grupos Tm(43±4%) e C(32±4%) que também diferiram entre si. Em relação às curvas de estiramento tensão, não foram encontradas diferenças entre os grupos, nos coeficientes angulares da TD e da rigidez miocárdica. Em conclusão, a variação da intensidade do programa de treinamento exerce influência tanto na capacidade física quanto no desempenho contrátil e pode ser atribuída à melhora dos mecanismos envolvidos na cinética do Ca EFEITOS DE EXPOSIÇÃO DA FUMAÇA DE CIGARRO DE CURTO PRAZO NA FUNÇÃO RENAL E MIOCÁRDICA EM RATOS ARIANA ALINE SILVA 1 ; LILIAN LUIZ DA SILVA ALVES 1 ; MAURO SÉRGIO PERILHÃO 1 ; RAFAEL LUIZ SILVA 2 ; EDNEI LUIZ ANTONIO 2 ; JAIRO MONTEMOR DA SILVA 2 ; ANDREY JORGE SERRA 3 ; LEONARDO DOS SANTOS 4 ; NESTOR SCHOR 2 ; PAULO JOSÉ FERREIRA TUCCI 2 ; DANILO SALES BOCALINI 1 1.UNIVERSIDADE SÃO JUDAS TADEU, MOÓCA, SP, BRASIL; 2.UNIFESP, SÃO PAULO, SP, BRASIL; 3.UNINOVE, SÃO PAULO, SP, BRASIL; 4.UFES, VITÓRIA, ES, BRASIL. Estudos recentes apresentaram efeitos diretos do tabagismo sobre o sistema cardiovascular. No entanto, as alterações miocárdicas e renais permanecem ainda inconclusivas. O objetivo deste estudo foi investigar os efeitos da exposição da fumaça do cigarro a curto prazo sobre a função miocárdica e renal de ratos. Para tanto, ratas Wistar foram randomizados em 2 grupos, controle (C) e 1 grupo experimental que foi exposto a fumaça de cigarro durante 8 semanas (CS). Após o período de exposição de 8 semanas (2 períodos totalizando 40 cigarros diários, 5 dias por semana), análises bioquímicas, funcionais e morfológicas foram realizadas através da ecocardiografia, contratilidade miocárdica e analise da urina. A massa do ventrículo esquerdo (2,22 ± 0,11 mg/g) e cardíaca (2,88 ± 0,15 mg/g) do grupo CS foi significativamente maior (P <0,05) em comparação ao grupo C (1,89 ± 0,12 mg/g; 2,52 ± 0,19 mg/g). Foram encontradas reduções significativas do diâmetro do ventrículo esquerdo do grupo CS na diástole (0.62 ± 0.03 mm) e na sístole (0.27 ± 0.03 mm) em relação ao grupo C (0.77 ± 0.09 mm; 0.40 ± 0.04 mm) respectivamente. Não foram encontradas diferenças nos parâmetros hemodinâmicos e da função do miocárdio e renal. Em conclusão, 8 semanas de exposição da fumaça do cigarro induzremodelação do miocárdio sem disfunção ventricular esquerda e anormalidades no desempenho contrátil do miocárdio, adicionalmente, o protocolo de exposição também não foi capaz de induzir alterações na função renal PREDICTORS AND REFERENCE VALUES OF PULSE WAVE VELOCITY IN PREPUBERTAL CHILDREN ANGOLAN AMÍLCAR BERNARDO TOMÉ DA SILVA 1 ; DANIEL PIRES CAPINGANA 2 ; PEDRO MAGALHÃES 3 ; JOSÉ GERALDO MILL 4 1.PPGCF-UFES, VITORIA, ES, BRASIL; 2.FAC. MEDICINA/UAN, LUANDA, ANGOLA; 3.FAC, MEDICINA/UAN, LUANDA, ANGOLA; 4.PPGCF/UFES, VITÓRIA, ES, BRASIL. Background: Carotid-femoral pulse wave velocity (cf-pwv) is the gold standard method used in clinical practice and epidemiological studies to determine arterial stiffness. However the lack of reference values for specific groups, difficult to identify the cutoffs for cardiovascular risk. The goal is to establish reference values for cf-pwv and determine its main predictors in prepubertal children. Methods: A cross-sectional, observational and descriptive study in a sample of black children enrolled in a public school of the 1st cycle of primary education in the urban area of Luanda, the Angolan capital. We evaluated 157 children 7-11 years of age (9.30 ± 1.1 years), Stage I of Tanner. To measure the cf-pwv was used an automatic device, brand Complior SP. Results: Mean cf-pwv was 4:59 ± 0:54 m/s with no difference between gender. Graphics with values of percentiles for height and age were presented. The univariate regression analysis model correlated positively cf-pwv (P <0.05) with age, weight, lean body mass, height, systolic blood pressure, diastolic and mean. Conclusion: After the multivariate analysis, only the height maintained as main predictors of PWV in prepubertal children. This is one of the few studies proposing reference values of PWV in this age group A EXPOSIÇÃO PRÉVIA A FUMAÇA DE CIGARRO INTENSIFICA O COMPROMETIMENTO DA CAPACIDADE FÍSICA E DA FUNÇÃO VENTRICULAR DE RATOS INFARTADOS MAURO SÉRGIO PERILHÃO 1 ; PAULA ANDRÉA MALVEIRA CAVALCANTE 1 ; PAULO JOSÉ FERREIRA TUCCI 2 ; JAIRO MONTEMOR DA SILVA 2 ; ANDREY JORGE SERRA 3 ; LEONARDO DOS SANTOS 4 ; EDNEI LUIZ ANTONIO 5 ; DANILO SALES BOCALINI 1 1.UNIVERSIDADE SÃO JUDAS TADEU, SÃO PAULO, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DE SAO PAULO, SÃO PAULO, SP, BRASIL; 3.UNIVERSIDADE NOVE DE JULHO, SÃO PAULO, SP, BRASIL; 4.UNIVERSIDADE FEDERAL DO ESPIRITO SANTO, VITORIA, ES, BRASIL; 5.UNIVERSIDADE FEDERAL DE SÃO PAULO, SÃO PAULO, SP, BRASIL. As repercussões da exposição à fumaça realizada previamente ao infarto ainda permanecem inconclusivas. O objetivo do estudo foi investigar os efeitos da exposição previa da fumaça de cigarro previamente ao infarto na capacidade física, na dilatação e na função ventricular. Oitenta e três ratas foram randomizadas em quatro grupos: controle (C:n=10), fumantes (F:n=18, expostos à fumaça), infarto (IM,n=26, submetidos cirurgia de oclusão coronária) e fumante infarto (FIM,n=29, expostos à fumaça e oclusão coronária). O protocolo de exposição de 16 semanas (40cigarros,2xdia,5xsem). Prejuízos foram encontrados no grupo F ( vs ). Após a interrupção da exposição o desempenho do grupo F foi recuperado, contudo foi menor que o grupo C (202+15) e semelhante aos grupos IMm (158+4) e FIMm (161+4) que não diferiram entre si. Ao analisarmos os grupos IMg diferenças estatísticas (p<,001) foram encontradas entre os demais grupos, entretanto, o grupo FIMg (110+5) apresentou desempenho inferior em relação ao grupo IMg (134+3). O período de exposição à fumaça de cigarro induziu o desenvolvimento de hipertrofia concêntrica com redução na FEAT. Após a interrupção da exposição os valores da FEAT dos grupos C e F foram maiores que os grupos IMm (32+6) e FIMm (36+7) que não diferiram entre si, mas foram superiores aos grupos IMg (21+4) e FIMg (17+4). Em conclusão animais submetidos à fumaça previamente ao infarto apresentam comprometimento na CF e na função ventricular ALTERAÇÔES DOS PARÂMETROS HEMATOLÓGICOS DE RATOS WISTAR: INFLUENCIA DO ENVELHECIMENTO MAURO SÉRGIO PERILHÃO 1 ; LILIAN LUIZ DA SILVA ALVES 1 ; ARIANA ALINE SILVA 1 ; WILSON JACOB FILHO 2 ; ROMEU SOUZA 1 ; DANILO SALES BOCALINI 1 1.UNIVERSIDADE SÃO JUDAS TADEU, SÃO PAULO, SP, BRASIL; 2.UNIVERSIDADE DE SÃO PAULO, SÃO PAULO, SP, BRASIL. Pesquisas com modelo animal têm sido amplamente utilizadas na avaliação dos efeitos do crescimento e envelhecimento de tecidos e órgãos, favorecendo o avanço do conhecimento dos mecanismos vitais, aperfeiçoamento dos métodos de prevenção, diagnóstico e tratamento de doenças. Objetivo: avaliar as alterações dos parâmetros hematológicos de ratos machos Wistar de acordo com o processo de envelhecimento.material e métodos: Para tanto foram estudados 140 ratos Wistar Machos, distribuídos em seis grupos: 2, 3, 6, 12, 18, e 24 meses de vida. Todos os animais foram previamente sedados e extraído amostras de sangue da extremidade distais da cauda, permitindo o livre fluxo de sangue, sendo a posteriori, preparados esfregaços para a contagem diferencial dos leucócitos e o percentual de reticulócitos. Resultados: no diferencial de Concentração de Hemoglobina Corpuscular Média (2: 32,18±2,44 ; 3:3,31±2,5; 6;31,48±1,17 ;12:31,18±1,02 ;18: 30,08±1,31; 24:35,46±1,53), Eosinofícilos(2: 0,6± 0,69; 3:0,93±1,03; 6:2,88±2,31 ;12: 2,16±1,160,91; 18:,8±2,28; 24; 4,2±2,86), linfócitos (2: 10,12±2,21 ; 3: 11,45±3,69 ; 6: 11,66±5,27;12: 11,98±5,87 ;18: 7,76±0,96; 24:10,56±7,05) e monócitos (2: 2,9±1,28; 3:3,06±2,31; 6:3,22±0,83;12: 2,33±1,36;18: 2,8±2,04;24: 2,4±1,14).Conclusão: Foram identificadas discretas alterações nos parâmetros hematopatologicos de acordo com o processo de envelhecimento, contudo, os dados do presente estudo aponta para o estabelecimento de valores hematológicos de referência para estudos com este tipo de modelo animal FETAL HEART PROGRAMMING BY PROTEIN RESTRICTION DIET: EFFECTS ON HEART STRUCTURE AND BLOOD PRESSURE IN MALE YOUNG ADULT OFFSPRING KAMILA FERNANDA ROSSINI; HÉRCULES JONAS REBELATO; MARCELO AUGUSTO MARRETO ESQUISATTO; ROSANA CATISTI FUNDAÇÃO HERMÍNIO OMETTO, ARARAS, SP, BRASIL. The dietary limitation during pregnancy influences the growth and development of the fetus and offspring and their health into adult life. The adverse effect of fetal protein restriction exposures and mechanisms underlying these associations on heart development and functional disorder are unclear. The present study investigates in young adult male rats, the effect of gestational protein restriction (GPR) on blood pressure (BP) by an indirect tail-cuff method using an electro sphygmomanometer and its association with heart structure histological techniques. Dams (n=12) were kept on normal (NP), or low (LP) protein diet over all pregnancy phase. BP of pups was measured from 4th to 8th week. The current study showed in at 8-week old LP offspring showed no difference in body weight, though newly born was lighter. Arterial BP enhanced significantly in LP (131.8±2.7 mmhg) than in NP (120.3±3.33 mmhg) animals. The myocardium did not present macroscopical alterations between groups on: heart/body weight (NP, mg/g; LP, 3.86±0.28 mg/g) and left ventricle/body weight (NP, 2.28±0.25 mg/g; LP, 2.49±0.27 mg/g). It was observed a significant decrease in the number of myocytes by 10 4 µm 2. The area occupied by collagen fibers increased significantly. These results suggest that GPR affects myocardium organization in male youngs adult offspring heart. Approved by CEUA/UNIARARAS (056/2014) and supported by FHO Uniararas VENTRICULAR REMODELING OF RATS FED AT LOW PROTEIN DIET IN LATE PREGNANCY ANA CLAUDIA APARECIDA TEIXEIRA; MARCELO AUGUSTO MARRETO ESQUISATTO; HÉRCULES JONAS REBELATO; ROSANA CATISTI CENTRO UNIVERSITÁRIO HERMÍNIO OMETTO, ARARAS, SP, BRASIL. The heart size is increased during pregnancy, but it is unclear if such process would be due to heart muscle hypertrophy or changes that result from the anatomic alterations which normally occurs in this period. This study investigated whether gestational protein restriction alters left ventricle hypertrophy at end of gestation. Pregnant dams were divided into two groups: normal (NP, 17% casein) or low-protein diet (LP, 6% casein). At day 21 of gestation (21 dg), after anestesia, the animals were perfused and the hearts collected, weighed and processed by Toluidine blue (myocytes quantification) and Picrossirius-hematoxylin (collagen fibers quantification). Cardiomyocytes cross-sectional area was determined by hematoxylin-eosin. The myocardium presented structural alterations in the LP group compared to NP. The results showed on 21dG a significant decrease in the number of myocytes by 104µm2. The area occupied by collagen fibers increased significantly at the end of gestation in LP dams. These results show that protein restriction affects myocardium organization in maternal heart and suggest degenerative process, compatible with fibrosis. Study approved by CEUA/UNIARARAS (062/2013) MECANISMOS SINÉRGICOS LEVANDO A UM MAIOR RISCO DE AVC EM MULHERES IDOSAS JULIANA STEFANIN FUZATTI; SANDRA GOMES DOS SANTOS; LUCIANA APARECIDA CAMPOS; VALTER LUIS PEREIRA JR.; OVIDIU CONSTANTIN BALTATU CAMILO CASTELO BRANCO UNIVERSITY (UNICASTELO), SAO JOSE DOS CAMPOS, SP, BRASIL. Doença carótida arteriosclerótica tem sido associada a um risco aumentado de acidente vascular cerebral. O objetivo deste estudo foi investigar a relação entre a doença carotídea e marcadores cardíacos e vasculares de risco não-ateroscleróticos em mulheres idosas. Doença carotídea foi determinada medindo-se o espessamento da carótida íntima-média (EIM). O EIM das carótidas esquerda e direita, o escore de placa e índice de resistência (IR da carótida comum, externa e interna), espessura da parede do ventrículo esquerdo (VE) e diâmetro da aorta ascendente foram determinados pela ultra-sonografia duplex e modo-b. A idade das mulheres e dos homens foi de 71 ± 11 e 68 ± 11, respectivamente. O EIM da carótida direita foi correlacionada com o escore de placa em ambos os sexos (r de Spearman = 0,36 em mulheres e 0,37 em homens, p = 0,01). O EIM da carótida direita teve uma correlação significantemente positiva com a espessura da parede do VE e do diâmetro da aorta ascendente só em mulheres (de Spearman r = 0,31 e 0,31, p <0,05), mas não em homens. O EIM da carótida direita, espessura do VE e diâmetro da aorta foram positivamente correlacionados com a idade apenas em mulheres (r de Spearman = 0,33, 0,40 e 0,26, respectivamente, p <0,05). Estes dados indicam que marcadores não-ateroscleróticos de risco como hipertrofia cardíaca, dilatação da aorta e idade estão associados com maior EIM em mulheres, mas não em homens. A ação sinérgica desses fatores pode contribuir para uma piora na deterioração da carótida em mulheres idosas NORADRENALINE INJECTED IN THE RETROTRAPEZOID NUCLEUS INHIBITS INSPIRATION LUIZ MARCELO OLIVEIRA; THIAGO S MOREIRA; ANA CAROLINA T TAKAKURA UNIVERSITY OF SÃO PAULO, SAO PAULO, SP, BRASIL. Retrotrapezoid nucleus (RTN) is a region located in the ventrolateral medulla and is known to contribute to central chemoreception, inspiration and active expiration. Previous studies showed the presence of catecholaminergic varicosities in the RTN region and also showed that noradrenaline (NA) can increase respiratory rate by the activation of α1 adrenergic receptors in the pre-bötzinger complex. The aim of this study was to investigate the cardiorespiratory effects produced by the injection of NA in RTN. Mean arterial pressure (MAP), diaphragm (DiaEMG) and abdominal (AbdEMG) muscle activities were recorded in urethane-anaesthetized, vagotomized and artificial ventilated male Wistar rats (n = 6-8, CEUA: 14, p.15, b.03/2014). Unilateral injection of NA produced a transient dose-dependent ( µm) inhibition of DiaEMG (26.4 ± 7.9%; 40.6 ± 8.1% and 65.0 ± 2.0%, respectively), but did not change MAP and AbdEMG. One minute after the highest dose (1000 µm) of NA into the RTN we observed a generation of sighs (larger amplitude in DiaEMG) (0.64 ± 0.07; vs. control: 0.33 ± 0.05 mv) that lasted for 286 ± 35 seconds. We conclude that the activation of adrenergic receptors in the RTN region inhibits inspiratory activity; and the highest dose of NA tested generated sighs during normal respiration activity SELECTIVE DENERVATION OF THE AORTIC AND CAROTID BARORECEPTORS 7

8 JACI AIRTON CASTANIA 1 ; PEDRO LOURENÇO KATAYAMA 1 ; FERNANDA BROGNARA 1 ; JOÃO PAULO JACOB SABINO 2 ; RUBENS FAZAN 1 ; HELIO CESAR SALGADO 1 1.FMRP/USP, RIBEIRAO PRETO, SP, BRASIL; 2.FORP/USP, RIBEIRAO PRETO, SP, BRASIL. The usual surgery for sinoaortic denervation (SAD) in rats leads to concomitant denervation of the carotid chemoreceptors. This study reports a new technique to denervate the carotid and aortic baroreceptors selectively maintaining the carotid chemoreceptors intact. Wistar rats were subjected to selective aortic and carotid baroreceptor denervation maintaining the carotid chemoreceptors intact (BAROS-X), or sham surgery (SHAM). Rats were implanted with femoral artery and vein catheters for arterial pressure recording and drug administration. Baroreflex or carotid chemoreflex activation was elicited by injection of phenylephrine or potassium cyanide (KCN). Phenylephrine caused hypertension in SHAM (Δ = 46 ± 3 mmhg) and BAROS-X (Δ = 38 ± 3 mmhg) rats combined with reflex bradycardia in SHAM rats (Δ = -54 ± 11 bpm) but no change in heart rate in BAROS-X (Δ = -5 ± 2 bpm) rats. The hypertensive (39 ± 5 mmhg in BAROS-X vs 44 ± 6 mmhg in SHAM rats) and bradycardic (-103 ± 23 bpm in BAROS-X vs -88 ± 17 bpm in SHAM rats) responses to KCN were similar in both groups confirming that the carotid chemoreceptors were intact. These results provide support to a new surgical technique in rats, to inactivate the aortic and carotid baroreceptors, keeping intact the carotid chemoreceptors STRUCTURAL ORGANIZATION OF THE HEART IN LATE PREGNANCY OF RATS FED 50% CALORIC RESTRICTION LARISSA RUELLA DE ASSIS; MARCELO AUGUSTO MARRETO ESQUISATTO; HÉRCULES JONAS REBELATO; DANIELLA NUNES ROLLO NEODINI; ROSANA CATISTI CENTRO UNIVERSITÁRIO HERMÍNIO OMETTO, ARARAS, SP, BRASIL. Pregnancy is characterized by left ventricular hypertrophy that is potentially accounted for by cardiomyocyte hypertrophy. The increase of maternal total blood volume and cardiac output during gestation may result from two mechanisms acting in concert: 1) the production of several hormones by the fetus and the placenta, and 2) the uteroplacental circulation acting as an arteriovenous shunt. This study investigated whether gestational caloric restriction alters left ventricle hypertrophy and collagen fibers distribution at end of gestation. Pregnant female Wistar rats were divided in control group (C, n = 6, basal diet) and restricted group fed 50% of the diet consumed by control (R, n = 6). At day 21 of gestation (21 dg), after anestesia, the animals were perfused and the hearts collected, weighed and processed by Toluidine blue (myocytes quantification) and Picrossirius-hematoxylin (collagen fibers quantification). Cardiomyocytes cross-sectional area was determined by hematoxylin-eosin and the results showed on 21dG a significant decrease in R group. The number of myocytes by 10 4 µm 2 were decreased in these animals. The area occupied by collagen fibers increased significantly at the end of gestation in restricted mothers. These results show that caloric restriction affects myocardium organization in maternal heart, clear evidence of a degenerative process. Study approved by CEUA/UNIARARAS (055/2014) T3 AND T4 EFFECTS IN INFLAMMATORY AND PRO-APOPTOTIC PROTEINS EXPRESSION AFTER MYOCARDIAL INFARCTION VANESSA DUARTE ORTIZ; ALEXANDRE LUZ DE CASTRO; RAFAEL DE OLIVEIRA FERNANDES; CRISTINA CAMPOS CARRARO; RAFAELA SIQUEIRA; JÉSSICA H.POLETTO BONETTO; ADRIANA CONZATTI; TÂNIA REGINA GATELLI FERNANDES; ADRIANE BELLÓ-KLEIN; ALEX SANDER DA ROSA ARAUJO UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL. Introduction: Several studies have demonstrated cardioprotective effects of thyroid hormones (TH) after myocardial infarction. Objective: The aim of this study was to evaluate TH effects on cardiac function after myocardial infarction, as well as evaluate inflammatory and pro-apoptotic proteins expression. Methods: Male Wistar rats ( g) (n=8/group) were divided into four groups: Sham-operated (SHAM), infarcted (AMI), sham-operated + TH (SHAMT) and infarcted + TH (AMIT). During 12 days, the animals received T3 and T4 (2 and 8 µg/100g/day) by gavage. After this, the rats were submitted to hemodynamic and echocardiographic analysis, were sacrificed and the left ventricle (LV) was collected to molecular analysis. Statistical analyses: two-way ANOVA with Student-Newman-Keuls post test. Ethics Committee number: Results: Ejection Fraction was increased in AMIT rats compared with AMI. LV systolic pressure was also increased and the end-diastolic pressure was reduced in AMIT when compared with AMI. The treated rats presented a decrease in toll like receptor 4 and in nuclear transcription factor kappa beta expression when compared with no treated groups. Besides that, AMI rats presented an increase in the pro-apoptotic proteins, p53 and JNK. The hormonal treatment prevented this increase. Conclusion: TH administration improved cardiac functional parameters, as well as decreased the expression of inflammatory and pro-apoptotic proteins after myocardial infarction THYROID HORMONES EFFECTS ON PERIPHERAL VASCULATURE AFTER ACUTE MYOCARDIAL INFARCTION VANESSA DUARTE ORTIZ; ALEXANDRE LUZ DE CASTRO; RAFAELA SIQUEIRA; JÉSSICA H.POLETTO BONETTO; ADRIANA CONZATTI; TÂNIA REGINA GATELLI FERNANDES; ADRIANE BELLÓ-KLEIN; ALEX SANDER DA ROSA ARAUJO UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL. Introduction: The treatment with thyroid hormones (TH) have presented positives effects over the myocardium after acute myocardial infarction (AMI), but there is no data regarding the influence of AMI and this treatment over the peripheral vasculature.objective: The main goal was to analyze the influence of these factors on the peripheral vasculature through parameters of oxidative stress, angiogenesis and TH receptors (TRα,TRβ) and endothelial nitric oxide synthase (enos) expression.methods: Male Wistar rats (~350g)(n=11-16/group) were divided into four groups: Sham-operated (SHAM), infarcted (AMI), sham-operated+th (SHAMT) and infarcted+th (AMIT). During 12 days, the animals received T3 and T4 (2 and 8µg/100g/day) by gavage. After, the rats were submitted to echocardiographic analysis. The aorta was collected to molecular analysis. Statistical analyses: two-way ANOVA with Student-Newman-Keuls post test. Ethics Committee number:23262.results: The vascular endothelial growth factor, hypoxia-inducible factor 1α, TRα and TRβ receptors expression increased in AMIT compared to AMI. The reactive oxygen species production, NADPH oxidase activity and enos expression decreased in AMI compared with SHAMT, while there is no deference between AMIT and AMI.Conclusion: The TH presented an action over angiogenesis and TRα and TRβ expression on peripheral vasculature in a post-ami period. These may indicate an improvement in angiogenesis and a better responsiveness of peripheral vasculature to HT CHRONIC UNPREDICTABLE STRESS INCREASES CARDIAC SYMPATHETIC MODULATION IN RATS WITH CHEMICALLY INDUCED OVARIAN FAILURE BRUNA BARCELOS DE SIMAS 1 ; FLAVIANO LORENZON 1 ; TAMIRES GREGORIO 1 ; JANETE APARECIDA ANSELMO-FRANCI 2 ; FERNANDA BARBOSA LIMA CHRISTIAN 1 ; DOMITILA AUGUSTA HUBER 1 1.UNIVERSIDADE FEDERAL DE SANTA CATARINA, FLORIANOPOLIS, SC, BRASIL; 2.UNIVERSIDADE DE SAO PAULO, RIBEIRAO PRETO, SP, BRASIL. Estrogen loss and stress leave women more susceptible to cardiovascular problems. Therefore, we sought to determine whether the exposure to chronic unpredictable stress (CUS) during ovarian failure induced by 4-vinylcyclohexene diepoxide (VCD) in rats could alter the autonomic control of the cardiovascular system. Female rats (28 days old) were treated with VCD (160 mg/kg, ip) or vehicle (CTL) for 15 consecutive days. After 70 days, the rats were exposed to daily sessions of CUS for 10 days. After that, catheters were inserted into the femoral artery and vein. Recordings of arterial pressure (AP) were performed 24 h later. Using the computer software CardioSeries v2.4, we evaluated the high (HF) and low frequency (LF) bands of pulse interval (PI) and LF band of systolic AP spectrum. Mean AP, HR and LF of systolic AP were comparable among groups. The spectral analysis revealed no difference between VCD (9) and CTL (7) groups with no stress. However, for the groups exposed to CUS, VCD (7) compared to CTL (6) showed an increase in LF (47±3 vs 31±3 nu, P<0.05) associated with a decrease in HF of PI spectrum (53±3 vs 68±3 nu, P<0.05), generating a higher LF/HF ratio (1.1±0.1 vs 0.6±0.1, P<0.05). Therefore, when exposed to stress, rats with chemically induced ovarian failure presented an increased sympathetic associated with a decreased parasympathetic modulation of cardiac function when compared to normal rats, leading to higher risks for cardiovascular diseases PAROXETINE IMPROVES CARDIAC CONTRACTILITY IN AORTIC REGURGITATION RATS BY GRK2 INHIBITION ANA CAROLINA MIEKO OMOTO 1 ; LEONARDO NAZARIO MORAES 1 ; GEYSSON JAVIER FERNANDEZ 1 ; MELIZA GOI ROSCANI 2 ; ROBSON FRANCISCO CARVALHO 1 ; JULIANA IRANI FRATUCCI DE GOBBI 1 1.IBB UNESP, BOTUCATU, SP, BRASIL; 2.UFSCAR, SÃO CARLOS, SP, BRASIL. Aortic regurgitation (AR) culminates with eccentric hypertrophy due to a volume overload. Cardiac hypertrophy is characterized by marked desensitization of β-adrenergic receptors (β-ar) and impairment of cardiac contractility. G protein coupled receptor kinase 2 (GRK2) activities are involved in dysfunctional adrenergic signaling. Paroxetine (parox), a consecrated selective serotonin reuptake inhibitor, improves systolic function in AR rats. Recently, another action was attributed to parox, it might act as a GRK2 inhibitor. Thus, we verify gene expression of GRK2 in AR rats treated with parox. Male Wistar rats ( kg) were submitted either to AR surgery, by retrograde puncture of the aortic valves leaflets, or sham surgery. The animals were divided in 4 groups: AR+parox, AR+saline, Sham+parox and Sham+saline. Parox (10mg/kg) was administered subcutaneously for 4 weeks and saline was used as control. Morphofunctional variables of the hearts were analyzed by echocardiograms at week 4 (before) and at week 8 (after) treatment. At week 8 the animals were euthanized for tissue collection and posterior analysis of the gene expression by RTq-PCR. Two way RM ANOVA was used for comparisons. The AR+parox group presented an improvement in fractional shortening and decreased the GRK2 gene expression (AR+parox: 1.14 ± 0.05 vs AR+saline: 1.39 ± 0.05). Our results show that parox treatment improves systolic function of AR rats probably due inhibition of GRK2 activity BAROREFLEX SENSITIVITY AND ADRENERGIC VASCULAR REACTIVITY IN RATS SUBMITTED TO CROSS-SENSITIZATION BETWEEN PERIMENOPAUSE AND STRESS TAMIRES GREGORIO 1 ; FLAVIANO LORENZON 1 ; BRUNA BARCELOS DE SIMAS 1 ; JANETE APARECIDA ANSELMO-FRANCI 2 ; DOMITILA AUGUSTA HUBER 1 ; FERNANDA BARBOSA LIMA CHRISTIAN 1 1.UNIVERSIDADE FEDERAL DE SANTA CATARINA, FLORIANOPOLIS, SC, BRASIL; 2.UNIVERSIDADE DE SAO PAULO, RIBEIRAO PRETO, SP, BRASIL. During perimenopause, the chance of developing cardiovascular disease increases, especially with the high incidence of stressors in this phase of life. Therefore, we studied cardiovascular parameters in an experimental model of perimenopause exposed to chronic unpredictable stress (CUS). Female rats (28 days old) were treated with 4-vinylcyclohexene diepoxide (VCD, 160 mg/kg, ip) or oil (CTL) for 15 consecutive days. After 70 days, the CUS was performed daily during 10 days and then catheters were inserted into femoral artery and vein. 24 h later, arterial pressure (AP) and heart rate (HR) were recorded, and the baroreflex was evaluated by bolus injection of phenylephrine (PE, 2 µg/kg) and sodium nitroprusside (SNP, 4 µg/kg). Hexamethonium (25 mg/kg) was used to study the effect of ganglionic blockade on mean AP. Then, different doses of PE were administered to access the adrenergic vascular reactivity -mediated pressor responses. The basal AP and HR were comparable among the groups: VCD (n=10, 115±2 mmhg), CTL (n=7, 110±3 mmhg), CUS-VCD (n=8, 117±3 mmhg), CUS-CTL (n=7, 110±3 mmhg). The tachycardia evoked by SNP and bradycardia evoked by PE did not differ between VCD and CTL, exposed to CUS or not. In addition, the contribution of sympathetic activity to basal AP and adrenergic vascular reactivity were comparable among the groups. In conclusion, during perimenopause, the exposure to stress does not produce changes in the studied parameters H9C2 CELL LINE: HOW RELIABLE IS IT? PRELIMINARY STUDY WITH PAROXETINE ANA CAROLINA MIEKO OMOTO; IVAN JOSE VECHETTI JR; LEONARDO NAZARIO MORAES; GEYSSON JAVIER FERNANDEZ; PATRICIA FIDELIS DE OLIVEIRA GREGOLINI; ROBSON FRANCISCO CARVALHO; JULIANA IRANI FRATUCCI DE GOBBI IBB UNESP, BOTUCATU, SP, BRASIL. The H9c2 is a clonal myoblastic cell line, which has been used to clarify molecular mechanisms involved in pathophysiological hypertrophic growth. It has been showed that paroxetine (parox) improves the contractility of the heart in cardiomyopathies. Therefore we treated H9c2 cells with parox to study its influence on some gene related to the pathophysiology of hypertrophy. Thus the gene expression of α-skeletal actin, ANP, BNP and the myosins isoforms (α-myhc and β-myhc) were investigated in H9c2 cells. Growth media (DMEM) was used as vehicle, cells were seeded in 2 plates with 6 wells; at 80% of confluence they were exposed to the treatments. Phenilefrine (phenil, 100μM), an inductor of hypertrophy, was added to the media for 24 hours and then parox (300 μm) was added for another 24 hours. The study was divided into 4 groups: phenil+parox, DMEM+parox, phenil+dmem and DMEM+DMEM. At the end of the experiments cells were extracted for molecular analyzes of gene expression, performed by RT-qPCR. There were no changes in the expression of α-skeletal actin, a hypertrophy marker. The ANP, BNP and the myosin isoform genes were not expressed in this cell line under our experimental conditions. Thus, H9c2 are undifferentiated cells, derived from embryonic rat heart, which must be strongly challenged in order to express characteristics of cardiomyocytes. Our present results arises some questions and restrictions on using this cell line to understand specific cardiomyocyte genes EFEITOS TARDIOS SOBRE RESPOSTAS HEMODINÂMICAS A AGENTES VASOATIVOS NA PROLE EXPOSTA À FLUOXETINA DURANTE GESTAÇÃO E LACTAÇÃO NATÁLIA KIMIE MATSUBARA 1 ; LUIZ FERNANDO VERÍSSIMO 1 ; VINICIUS LUCCA VOLPINI 1 ; VIVIANE BATISTA ESTRADA 1 ; DENIS CARLOS DOS SANTOS 1 ; ESTEFÂNIA GASTALDELLO MOREIRA 1 ; DANIELA CRISTINA CECCATTO GERARDIN 1 ; MARCUS VINICIUS DE MATOS GOMES 2 ; GRAZIELA SCALIANTI CERAVOLO 1 ; LEONARDO RESSTEL BARBOSA MORAES 3 ; GISLAINE GARCIA PELOSI 1 1.UNIVERSIDADE ESTADUAL DE LONDRINA, LONDRINA, PR, BRASIL; 2.UNIVERSIDADE NORTE DO PARANÁ, LONDRINA, PR, BRASIL; 3.FACULDADE DE MEDICINA DE RIBEIRÃO PRETO/ UNIVERSIDADE DE SÃO PAULO, RIBEIRÃO PRETO, SP, BRASIL. A fluoxetina (FLX), um antidepressivo do tipo inibidor seletivo da recaptura de serotonina, é utilizada para o tratamento da depressão, inclusive durante a gravidez. Ela penetra a barreira hematoplacentária, expondo fetos e neonatos em desenvolvimento, mas os efeitos no sistema cardiovascular da prole exposta à FLX ainda não são desconhecidos. Este estudo avaliou os efeitos da exposição materna à FLX sobre respostas hemodinâmicas a agentes vasoativos na prole. Ratas Wistar receberam por gavagem FLX (5mg/kg; FLX) ou água (CTR), da gestação até o desmame. No dia pós-natal inseriu-se na prole um cateter na artéria e veia femoral, para registro da pressão arterial (PA) e infusão de fenilefrina (FE), nitroprussiato de sódio (NPS) e acetilcolina (Ach). No dia seguinte, registrou-se a PA por 10min, seguido de infusão das drogas para análise hemodinâmica. Para análise da curva dose-resposta usou-se teste ANOVA de duas vias e pós teste Bonferroni. O Emáx e ED50 foram comparados com teste t de Student não pareado. Dados em média ± EPM e P<0,05. Houve aumento da velocidade de resposta para FE e NPS, mas sem alterar o ED50. Também houve mudanças no Emáx com Ach (FLX:-32,5±3,1, n=9; CTR:-42±3,23, n=9; P=0,0492, T=2,128) e NPS (FLX:-56,33±3,407, n=14; CTR:-45,89±1,524, n=15; P=0,008, T=2,863). Não houve mudanças na resposta hemodinâmica em fêmeas. Os resultados mostram que a exposição à FLX altera respostas hemodinâmicas dependente de gênero ESTUDO DAS ALTERAÇÔES CARDÍACAS ENCONTRADAS EM CAMUNDONGOS FÊMEAS QUE SUPEREXPRESSAM VACHT VANESSA PEREIRA TEIXEIRA; MARIANA GAVIOLI DE OLIVEIRA; MARCOS BARROUIN MELO; SILVIA CAROLINA GUATIMOSIM FONSECA UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL. O estrógeno é conhecido por promover efeitos cardioprotetores, sendo que um dos seus mecanismos se dá pela modulação do sistema nervoso autônomo. Neste contexto, dados obtidos pelo nosso grupo mostraram que camundongos com redução na atividade parassimpática, estão protegidos dos efeitos deletérios da ovariectomia no coração. O objetivo deste trabalho foi investigar se camundongos fêmeas que apresentam hiperatividade colinérgica, devido a superexpressão do VAChT (SVT), apresentam alterações cardíacas e sua relação com estrógeno. Para a realização desse estudo foram utilizados camundongos fêmeas selvagens (WT) e SVT com 3 meses de idade. Ambos os grupos apresentam ciclos estrais regulates. Análise ecocardiográfica evidenciou que camundongos SVT apresentam aumento da fração de ejeção e de encurtamento do ventrículo esquerdo, quando comparados aos WT. Foi realizada a coleta e pesagem do útero, apresentando o grupo WT um maior peso com relação ao grupo SVT. Western blot para análise de proteínas cardíacas mostrou que animais SVT apresentam aumento na expressão da SERCA e 8

9 redução da fosforilação da fosfolambam no resíduo Thr17, quando comparados aos WT. Corações SVT também apresentam menor expressão da Monoamina oxidase (MAO) e maior expressão de Tirosina Hidroxilase no resíduo Ser40 com relação ao grupo WT. Além disso, corações de camundongos SVT apresentam alteração na expressão de receptores de estrógenos. Em conjunto nossos dados sugerem que a superexpressão do VAChT leva a alteração nos parâmetros modulados pelo estrógeno e maior contratilidade do músculo cardíaco NANOCAPSULES WITH COPAIBA OIL PROMOTES CARDIOPROTECTION IN A MODEL OF PULMONARY HYPERTENSION CRISTINA CAMPOS CARRARO 1 ; ANGELA MARIA VICENTE TAVARES 1 ; RAFAEL DE OLIVEIRA FERNANDES 1 ; RAFAELA SIQUEIRA 1 ; ALEXANDRE LUZ DE CASTRO 1 ; GIANA BLUME CORSSAC 1 ; TÂNIA REGINA GATELLI FERNANDES 1 ; CLAUDIO PEREIRA 2 ; ALEX SANDER DA ROSA ARAUJO 1 ; ADRIANE BELLÓ-KLEIN 1 1.UFRGS, PORTO ALEGRE, RS, BRASIL; 2.TECNANO, PORTO ALEGRE, RS, BRASIL. The aim of this study was to determine the antioxidant potential of copaiba oil and nanocapsules containing this oil in a pulmonary arterial hypertension (PAH) model. Male Wistar rats (170g, n=5/group) were divided in 6 groups: control,monocrotaline (MCT), copaiba oil (CO), copaiba oil + monocrotaline (CO+MCT), nanocapsule with Copaiba (NCO) and nanocapsule + monocrotaline (NCO+MCT). Animals received oil, nanocapsules or vehicle (by gavage) during 7 days. Then, PAH was induced by MCT (60 mg/kg i.p.). Echocardiographic measurements were performed 21 days after MCT injection. Afterwards, rats were killed to collect heart to evaluate morphometry and to perform tiorredoxin (Trx), peroxirredoxin (Prx) and glutarredoxin (Grx) protein expression by western blot.both oil and copaiba nanocapsules significantly (P<0.05) reduced RV hypertrophy. However, there was no significant difference between treatment with copaiba oil and nanocapsules. Trx and Prx protein expression were significantly increased in MCT group, and it was decreased in CO+MCT and NCO groups. Grx results showed no significant differences among groups. Data suggest that both, copaiba oil and nanocapsules were able to reduce right ventricular hypertrophy and increase antioxidant deffences in this model of PAH. This cardioprotective mechanism needs to be further investigated CARDIOVASCULAR CHANGES INDUCED BY PROTOTYPE ADMINISTRATION LQFM 163 STEFANNE MADALENA MARQUES; CASSIA MARIZ DA SILVA; NATHALIA ODA AMARAL; RICARDO MENEGATTI; GUSTAVO RODRIGUES PEDRINO UNIVERSIDADE FEDERAL DE GOIÁS, GOIANIA, GO, BRASIL. Objective: The LQFM 163 is a prototype drug similar to cilostasol. It has been demonstrated the use of cilostazol for treatment of peripheral vascular disease. The present study evaluated cardiovascular effects of LQFM 163 in normotensive rats. Methods: Wistar rats were anesthetized with urethane (1.2 mg/kg; b.wt.) and instrumented to mean arterial pressure (MAP), heart rate (HR) and aortic vascular conductance (AVC) recordings. The LQFM (1.66, 3.32, 6.65 mg/kg, b.wt., i.v.; in 0.1 ml) or vehicle (Tween 2%,i.v.; in 0.1 ml) was infused through the femoral vein cannula. Results: In anesthetized rats (n=6), infusion of vehicle (Tween 2%) do not promote changes in MAP (-1 ± 0.2 mmhg), HR (-2 ± 0.8 bpm) and AVC (3 ± 0.5%). Intravenous infusion of LQFM 163 produced significant hypotension (-13 ± 1.5mmHg) and aortic vasodilation (11 ± 2.5%) and do not promote changes in HR (-3 ± 5.4bpm) in dose of 6.65 mg/kg. Conclusion: The prototype LQFM 163 has potential utility and hypotensive vasodilator, however, further studies are needed to better characterize cardiovascular changes EXOGENOUS L-ARGININE REDUCES METALLOPROTEINASES ACTIVITY AND OXIDATIVE STRESS IN HYPERTENSIVE PATIENTS VINICIUS PACHECO GARCIA 1 ; HELENA NALY MIGUENS ROCHA 1 ; GUSTAVO MATARUNA DA SILVA 1 ; IGOR RIBEIRO DA COSTA CARDOSO 1 ; GABRIEL MATHEUS DA SILVA BATISTA 1 ; DANIEL GALINIS VIEIRA LIMA 1 ; ANTONIO CLAUDIO LUCAS DA NÓBREGA 1 ; LAURO CASQUEIRO VIANNA 2 ; NATÁLIA GALITO ROCHA 1 1.UNIVERSIDADE FEDERAL FLUMINESE, NITEROI, RJ, BRASIL; 2.UNIVERSIDADE DE BRASÍLIA, BRASÍLIA, DF, BRASIL. Aim: Defective L-arginine nitric oxide pathways in hypertension patients are associated with imbalances in metalloproteinases (MMPs) metabolism and redox homeostasis. We determined the effects of exogenous L-arginine infusion on endothelial biomarkers in hypertensive patients. Methods: Blood samples were drawn from seven hypertensive (HT; 45±5yr) and eleven normotensive men (CT; 37±3yr) before and during intravenous infusion of L-arginine. Serum NO (NO analyzer), MMP-2 and MMP-9 activities (gelatin zymography), tissue inhibitor of metalloproteinases-1 (TIMP-1, ELISA) and superoxide dismutase activity (SOD, ELISA) were measured. Thiobarbituric acid reactive substances (TBARS) were quantified as a proxy of oxidative stress. Results: At baseline, NO, TIMP-1 and MMP-2 and SOD activities were similar between groups (P>0.05), while TBARS and MMP-9 were higher in the HT (P=0.02). During L-arginine infusion, NO increased only in the CT (P=0.01 vs. baseline; P=0.03 vs. HT), while MMPs activities and MMP- 9/TIMP-1 ratio decreased only in HT (P 0.02). Yet, the increase of NO concentration (infusion minus baseline) was negatively associated with baseline systolic blood pressure (P=0.04) in HT. Although L-arginine infusion has normalized TBARS levels in HT (P=0.13), the SOD activity was higher in CT (P=0.01). Conclusion: Despite impaired L-arginine-NO pathway, exogenous L-arginine diminishes MMPs activity and MMP-9/TIMP-1 ratio as well as normalizes oxidative stress in hypertensive patients HYPOTENSION AND VASODILATASION RESPONSES INDUCED BY THE LQFM 47 NATHALIA ODA AMARAL UNIVERSIDADE FEDERAL DE GOIÁS, GOIANIA, GO, BRASIL. Objective: LQFM 47 compound was synthesized from cilostazol compounds. Studies suggest that cilostazol be used to peripheral vascular diseases treatment. The present study evaluated the cardiovascular effects of LQFM 47 in normotensive rats. Methods: Wistar rats were anesthetized with urethane (1.2 mg/kg; b.wt.) and instrumented to mean arterial pressure (MAP), heart rate (HR) and aortic vascular conductance (AVC) recordings. The LQFM (0.42, 0.83 and 1.66 mg/kg, b.wt., i.v.; in 0.1 ml) or vehicle (Tween 2%, i.v.; in 0.1 ml) was infused through the femoral vein cannula. Results: In anesthetized rats (n=6), infusion of vehicle (Tween 2%) do not promote changes in MAP (-3 ± 1.4 mmhg), HR (-4 ± 1.5 bpm) and AVC (3 ± 2.0 %). Intravenous infusion of LQFM 47 produced significant hypotension (-20 ± 4.9 mmhg) and aortic vasodilation (12 ± 8.6%) and do not promote changes in HR (20 ± 9.8 bpm) in dose of 1.66 mg/kg. Conclusion: Taken together, the result demonstrated that LQFM 47 is an compound that induces hypotension and aortic vasodilatation in normotensive rats. However further studies are required to identify the mechanisms involved in the vasodilation and hypotensive responses CARDIOVASCULAR RESPONSES INDUCED BY INTRAVENOUS ADMINISTRATION OF LQFM 96 IN RATS CASSIA MARIZ DA SILVA UNIVERSIDADE FEDERAL DE GOIÁS (UFG), APARECIDA DE GOIANIA, GO, BRASIL. Objective: LQFM 96 was synthesized from cilostazol compound. Studies suggest that cilostazol is a vasodilator drug used to prevent cerebrovascular accident. The present study evaluated the cardiovascular effects of LQFM 96 in normotensive rats. Methods: Wistar rats were anesthetized with urethane (1.2 mg/kg; b.wt.) and instrumented to mean arterial pressure (MAP), heart rate (HR) and aortic vascular conductance (AVC) recordings. The LQFM 96 (1.66, 3.32, 6.65 and mg/kg, b.wt., i.v.; in 0.1 ml) or vehicle (Tween 2%, i.v.; in 0.1 ml) was infused through the femoral vein cannula. Results: In anesthetized rats (n=6), infusion of vehicle (Tween 2%) do not promote changes in MAP (-1 ± 0.5 mmhg), HR (-1 ± 0.7 bpm) and AVC (4 ± 1.0 %). Intravenous infusion of LQFM 96 produced significant hypotension (-12 ± 2.4 and 23 ± 4.4 mmhg) and aortic vasodilation (19 ± 4.0% and 17 ± 3,0%) in doses of 6.65 and mg/kg, respectively. No changes were observed in HR after LQFM 96 administration (1 ± 1.1 bpm and -10 ± 10.0 bpm). Conclusion: The results demonstrated that LQFM 96 induced hypotension and aortic vasodilatation in normotensive rats. New experiments are required to identify the mechanisms involved in the vasodilation and hypotensive responses DIFFERENCES IN CA2+ HANDLING IN RAT ATRIAL AND VENTRICULAR MYOCYTES JAIR TRAPÉ GOULART; JOSÉ WM BASSANI; ROSANA ALMADA BASSANI CENTRO DE ENGENHARIA BIOMÉDICA E DEP. DE ENGENHARIA BIOMÉDICA/FEEC, UNIVERSIDADE ESTADUAL DE CAMPINAS, CAMPINAS, SP, BRASIL. The aim of this study was to investigate atrium vs. ventricle differences in Ca 2+ transients and fluxes, and in the contribution of different systems to Ca 2+ transport. Adult rat atrial and ventricular myocytes were isolated after cardiac perfusion with collagenase (CEUA/IB/UNICAMP, No ). Cells were electrically stimulated under perfusion with modified Tyrode s solution at 23 C. Cytosolic Ca 2+ concentration ([Ca 2+ ]i) was measured with indo-1. Ca 2+ fluxes were estimated according to Bassani et al. (Gen Physiol Biophys 2012; 31:401) to calculate the relative contribution to [Ca 2+ ]i decline of the sarcoplasmic reticulum (SR) Ca 2+ uptake, the Na + /Ca 2+ exchanger (NCX) and the slow remaining transporters, and the fractional SR Ca 2+ release during a twitch (FR). Data were compared with Mann-Whitney or Student s t test. Although twitch Ca 2+ transient amplitude and FR were not statistically different, atrial myocytes showed faster twitch [Ca 2+ ]i decline (half time: 102±9 vs. 148±10 ms; N= 16; p<0.01) and greater SR Ca 2+ content (164±5 vs. 135±6 µm; p<0.01). The SR contribution to Ca 2+ transport was greater in atria (93.0±0.6 vs. 82.7±1.9%; p<0.01), whereas NCX contribution was larger in the ventricle (15.4±1.9 vs. 4.8±0.4%; p<0.01). It thus seems that the contribution of transsarcolemmal Ca 2+ fluxes to excitationcontraction coupling and relaxation is greater in ventricular cells, probably due to the greater surface-to-volume ratio conferred by the presence of T-tubules, which are absent in atria ACUTE AORTIC REGURGITATION AND THE AUTONOMIC MODULATION TO THE HEART PALOMA GRAZIELE BITTENCOURT DA SILVA 1 ; ANA CAROLINA MIEKO OMOTO 1 ; RUBENS FAZAN 2 ; JULIANA IRANI FRATUCCI DE GOBBI 1 1.DEPT. OF PHYSIOLOGY, INSTITUTE OF BIOSCIENCE - UNESP BOTUCATU, BOTUCATU, SP, BRASIL; 2.DEPT. OF PHYSIOLOGY, SCHOOL OF MEDICINE, USP, RIBEIRAO PRETO, SP, BRASIL. Aortic regurgitation (AR) is a diastolic reflux of blood to the left ventricle. Acute AR has a poor prognostic and it s caused by bacterial endocarditis. There s still missing data in literature about how the autonomic nervous system acts in this situation. So we evaluated hemodinamic parameters, autonomic modulation, heart rate (HR) variability and baroreflex sensitivity (BRS) in AR rats. For this purpose, Wistar rats (250g-280g) were submitted to sham or AR surgery, done by retrograde puncture of the leaflets. After a week, all the animals received a catheter into the femoral artery (to register hemodynamic data), and another into the jugular vein (drug administration). The autonomic parameters were evaluated through double blockage with atropine (3mg/kg) and atenolol (8mg/kg). Intrinsic heart rate (IHR) was determined after two consecutives days of double blockage.the analisys of HR variability were done by CardioSeries program. AR rats presented lower diastolic pressure (70 ± 6 vs sham 99 ± 5 mmhg) and there weren t any other diferences in hemodinamics and autonomics parameters. IHR were similar between the groups (364 ± 11 vs sham 419 ± 23 bpm), and so was HR variability. SBR was lower in AR acute rats (1,13 ± 0,11 vs sham 1,64 ±0,14,ms/mmHg). Acute AR did not alter hemodinamic parameters, however determined a decrease in spontaneous barorroflex sensitivity, sugesting a damage in this defense mechanism to the heart ETHANOL WITHDRAWAL-INDUCED HIPERTENSION AND OXIDATIVE STRESS: ROLE OF AT1 RECEPTORS NATÁLIA ALMEIDA GONZAGA; CARLOS RENATO TIRAPELLI UNIVERSITY OF SAO PAULO, RIBEIRAO PRETO, SP, BRASIL. Ethanol withdrawal activates the renin-angiotensin system and systemic concentrations of angiotensin II. The aim of our study was to evaluate the role of AT1 on ethanol withdrawal-induced cardiovascular disorders. Male Wistar rats (250g) were divided into 6 groups: Control: animals received water ad libitum for 21 days and daily gavage (DG) of vehicle (CV) or losartan (LST- 10mg/kg/day - CL); Ethanol: animals were treated with an ethanol 9% (v/v) for 21 days and DG of vehicle (EV) or LST (EL); Ethanol withdrawal: animals were treated in the same way that ethanol group for 21 days and after that the ethanol was removed and the animals received water ad libitum until the 23 rd, and during ethanol withdrawal period (48h) DG of vehicle (EWV) or LST (EWL) (CEUA Protocol: ). To analyze the results we used one-way ANOVA followed by Tukey (*p<0.05). Ethanol withdrawal induced an increase of systolic blood pressure (mmhg; n=10) that was prevented by LST (C/V=121±1; CL=122±0.8; EV=121±1 EL=122±0.8; EWV=154±1*; EWL=122±1). In the plasma, ethanol withdrawal induced an increase of TBARS (nmol/ml; n=9) that was prevented by LST (CV=10±1; CL=12±1; EV=13±1; EL=14±1; EWV=20±2*; EWL=9±1). In the aorta, ethanol withdrawal induced an increase in O2 - (RLU/mg protein; n=8) that was prevented by LST (CV=248±19; CL=214±18; EV=228±18; EL=214±21; EWV=655±51*; EWL=199±24). Aortic catalase activity was not altered by ethanol withdrawal. Ethanol withdrawal increases the systolic blood pressure and oxidative stress and these responses are mediated by AT1 receptors INVOLVEMENT OF THE MEDIAN PREOPTIC NUCLEUS IN TONIC CONTROL OF THE RENOVASCULAR HYPERTENSION ALINE ANDRADE MOURAO 1 ; MARINA CONCEIÇÃO DOS SANTOS MOREIRA 1 ; ARYANNE BATISTA SOARES DE MELO 1 ; GRAZIELA TORRES BLANCH 2 ; ANDRE HENRIQUE FREIRIA-OLIVEIRA 1 ; GUSTAVO RODRIGUES PEDRINO 1 1.UNIVERSIDADE FEDERAL DE GOIÁS, GOIANIA, GO, BRASIL; 2.PONTIFÍCIA UNIVERSIDADE CATÓLICA DE GOIÁS, GOIANIA, GO, BRASIL. Renovascular hypertension is directly related to sympathetic hyperactivation. Recently, our laboratory demonstrated the participation of the Median Preoptic Nucleus (MnPO) in tonic control of sympathetic activity in normotensive and spontaneously hypertensive rats. However whether this nucleus participates of the hypertension maintenance in the renovascular model remains to be clarified. The present study sought to determine the effect of the MnPO inhibition in the renovascular hypertension. Male Wistar rats ( g) were submitted to the implantation of a silver clip around the left renal artery to induce 2-kidney 1-clip renovascular hypertension. Five weeks later, the rats were instrumented for mean arterial pressure (MAP), heart rate (HR) and renal sympathetic nerve activity (RSNA) records and for nanoinjections of saline (NaCl; 150 mm) and muscimol (GABAA agonist; 4 mm) into the MnPO. In 2K1C rats (n=6) systolic arterial pressure increased five weeks after the implantation of the silver clip, when compared to normotensive rats (208.76±15.09 mmhg vs ±14.72 mmhg; p<0.05). In 2K1C rats, the muscimol nanoinjections (n=6) promote significantly decreases of MAP (-17.9±3.4 mmhg), HR (-13.3±5.6 bpm) and RSNA (-13.8±2.5 %) when compared to the saline nanoinjections (n=6; MAP -0.2±0.6 mmhg, HR 2.6±0.9 bpm and RSNA -0.8±1.1 %). In summary, these results demonstrate the MnPO participation in autonomic and cardiovascular tonic control in renovascular hypertension CHRONIC ADMINISTRATION OF CATALASE INHIBITOR DECREASES SYMPATHETIC MODULATION IN RATS WITH RENOVASCULAR HYPERTENSION. MARIANA RUIZ LAUAR; LAURIVAL ANTONIO DE LUCA JR; PATRICIA MARIA DE PAULA; DEBORA SIMÕES ALMEIDA COLOMBARI; EDUARDO COLOMBARI; CARINA A.F. ANDRADE; JOSÉ VANDERLEI MENANI DEPARTMENT OF PHYSIOLOGY AND PATHOLOGY, DENTISTRY SCHOOL, SÃO PAULO STATE UNIVERSITY (UNESP), ARARAQUARA, SP, BRASIL. Recent data have shown that chronic subcutaneous (sc) administration of the catalase inhibitor 3- amino-1,2,4-triazol (ATZ) reduces blood pressure (BP) in 2-kidneys, 1-clip (2K1C) hypertensive rats. In the present study, we analyzed the autonomic modulation of cardiovascular function in 2K1C hypertensive rats treated with chronic sc injection of ATZ. Male Holtzman rats (initial weight g, n=7-11/group) received a silver clip around the left renal artery to generate 2K1C hypertension. After 6 weeks of the surgery, rats were treated with ATZ or saline for 7 days before the recording of arterial pressure and the analysis of systolic blood pressure (SBP) and pulse interval (PI) variability using power spectral analysis. Baseline MAP was increased in 2K1C rats compared to sham (207 ± 9, vs. 100 ± 2 mmhg, respectively). ATZ (600 mg/kg/day) reduced the low frequency (LF) of SBP (3.3 ± 0.5, vs. saline 7.6 ± 1.5 mmhg2) and LF of PI (14.6 ± 3, vs. saline: 31 ± 3 normalized units) in 2K1C hypertensive rats. ATZ increased high frequency (HF) of PI (85 ± 3, vs. saline: 68 ± 3 normalized units) and improved the sympathovagal balance calculated for LF/HF ratio of PI variability (0.2 ± 0.05, vs. saline: 0.52 ± 0.09). The results suggest that the treatment with ATZ reduces BP in 2K1C hypertensive rats by reducing the modulation of sympathetic activity. 9

10 INCREASED EXPRESSION OF MACROPHAGE MIGRATION INHIBITORY FACTOR IN THE NUCLEUS OF SOLITARY TRACT ATTENUATES THE RENOVASCULAR HYPERTENSION RAFAELA MOREIRA BARBOSA 1 ; GUILHERME F.F. SPERETTA 1 ; ANDRE HENRIQUE FREIRIA-OLIVEIRA 2 ; HONGWEI LI 3 ; COLIN SUMNERS 4 ; JOSÉ VANDERLEI MENANI 1 ; EDUARDO COLOMBARI 1 ; DEBORA SIMÕES ALMEIDA COLOMBARI 1 1.DEPARTMENT OF PHYSIOLOGY AND PATHOLOGY, SCHOOL OF DENTISTRY, SÃO PAULO STATE UNIVERSITY (UNESP), ARARAQUARA, SP, BRASIL; 2.CENTER FOR NEUROSCIENCE AND CARDIOVASCULAR PHYSIOLOGY, FEDERAL UNIVERSITY OF GOIÁS, GOIÂNIA, GO, BRASIL; 3.SOUTHERN MEDICAL UNIVERSITY, GUANGZHOU, CHINA; 4.DEPARTMENT OF PHYSIOLOGY AND FUNCTIONAL GENOMICS, COLLEGE OF MEDICINE, UNIVERSITY OF FLORIDA, GAINESVILLE, ESTADOS UNIDOS. Macrophage migration inhibitory factor (MIF) is an intracellular inhibitory regulator of the actions of angiotensin II (ANG II) in the central nervous system. ANG II actions at the nucleus of the solitary tract (NTS) are important for the maintenance of hypertension in renovascular hypertensive 2- kidney, 1-clip (2K1C). Therefore, we tested if increased expression of MIF in the NTS of 2K1C rats could alter the maintenance of hypertension and improve baroreflex function in these animals. MIF expression in the NTS was increased by AAV2-CBA-MIF microinjection and AAV2-CBA-eGFP served as a control vector. Vectors were injected 3 weeks after renal clip placement. MIF injections in the NTS attenuated the hypertension in 2K1C rats from 2 weeks after viral transduction until the end of the experiment (4 weeks after viral transduction), (2K1C-MIF: 145 ± 7, vs. 2K1C-eGFP: 176 ± 9 mmhg). MIF into the NTS also improved the reflex bradycardia (2K1C- MIF slope: -1.4 ± 0.3, vs. 2K1C-eGPF slope: ± 0.3 bpm/mmhg) and reflex tachycardia (2K1C- MIF slope: -4.7 ± 0.6, vs. 2K1C-eGPF slope -1.7 ± 0.3 bpm/mmhg). MIF overexpressed in the NTS in normotensive rats produced no change in arterial pressure or baroreflex function. These results suggest that increased expression of MIF in the NTS attenuates the hypertension and improves baroreflex function in 2K1C rats, possibly through anti-ang II action ANGIOTENSIN TYPE 2 RECEPTOR ACTIVATION WITHIN THE ROSTRAL VENTROLATERAL MEDULLA REDUCES BLOOD PRESSURE IN 2K1C HYPERTENSIVE RATS JESSICA L.B. MIURA 1 ; RAFAELA MOREIRA BARBOSA 1 ; JOSÉ VANDERLEI MENANI 1 ; COLIN SUMNERS 2 ; EDUARDO COLOMBARI 1 ; DEBORA SIMÕES ALMEIDA COLOMBARI 1 1.DEPARTMENT OF PHYSIOLOGY AND PATHOLOGY, SCHOOL OF DENTISTRY, SÃO PAULO STATE UNIVERSITY (UNESP), ARARAQUARA, SP, BRASIL; 2.DEPARTMENT OF PHYSIOLOGY AND FUNCTIONAL GENOMICS, COLLEGE OF MEDICINE, UNIVERSITY OF FLORIDA, GAINESVILLE, ESTADOS UNIDOS. Previous studies have demonstrated that intracerebroventricular infusion of the selective angiotensin II type 2 receptor (AT2R) agonist (Compound 21; C21) reduced mean arterial pressure (MAP) in normotensive rats (Gao et al, Am; J. Hypert., 24(6): 724, 2014). The rostral ventrolateral medulla (RVLM) is an important structure for regulating sympathetic outflow and blood pressure. AT2R overexpression in the RVLM reduces MAP in normotensive rats (Gao et al, Hypertension, 51(2): 521, 2008). Renovascular hypertensive 2-kidney, 1-clip (2K1C) rats exhibit increased activity of the renin-angiotensin system and pronounced sympathoexcitation. Therefore, we tested if bilateral microinjection of C21 into the RVLM of 2K1C rats could induce cardiovascular changes. C21 (200 nl of 100 µm) was injected bilaterally 6 weeks after renal clip insertion. MAP and heart rate (HR) were measured in freely moving sham (n = 5) and 2K1C rats (n = 5). C21 microinjections in the RVLM decreased the MAP in 2K1C rats (before-c21: 169 ± 9, vs. after-c21: 152 ± 8 mmhg), but not in sham rats (before-c21: 104 ± 3, vs. after-c21: 104 ± 3 mmhg). Similarly, injection of C21 into the RVLM reduced HR in 2K1C rats (before-c21: 336 ± 15, vs. after-c21: 300 ± 6 bpm), but not in sham rats (before-c21: 346 ± 12, vs. after-c21: 332 ± 12 bpm). These results suggest that AT2R activation in the RVLM is effective in reducing MAP and HR in 2K1C rats HIGH FAT-DIET INDUCES MICROGLIA ACTIVATION AND ASTROGLIOSYS IN THE IN THE NUCLEUS OF THE SOLITARY TRACT IN RATS GUILHERME FLEURY FINA SPERETTA; MARIANA DEL ROSSO MELO; MIRIAN BASSI; JOSÉ VANDERLEI MENANI; EDUARDO COLOMBARI; DEBORA SIMÕES ALMEIDA COLOMBARI UNESP/ARARQUARA, ARARAQUARA, SP, BRASIL. Neuroinflammation seems to be a mechanism involved in the obesity-induced cardiovascular disturbers. In the present study, we evaluated the microglia activation and astrogliosys in the in the nucleus of the solitary tract in HFD feeding rats. Male Holtzman rats ( g, n = 7/group) were fed with either standard rat chow diet (SD; 5.4% total fat) or HFD (26.4% total fat) for 6 weeks. Coronal sections (30 µm) of the brainstem were sectioned on a cryostat and incubated with anti Iba-1 and anti-gfap antibodies to label respectively, microglia and astrocytes. For the assessment of microglial number, 20 μm z-stacks were counted bilaterally by hand each 120 µm, in matched representative sections of the NTS. For assessment of GFAP immunoreactivity, images were converted into greyscale and binary formats and thresholds for black and white balance were adjusted to the same level in matched representative sections of the NTS. Rats fed with HFD exhibited higher immunoreactivity for GFAP (162 ± 9 vs SD: 133 ± 3 and 140 ± 5 vs. SD: 119 ± 5 area of GFAP staining, p < 0.05) and higher number of Iba-1 positive cells (23 ± 2 vs. SD: 16 ± 1 and 24 ± 2 vs. SD: 17 ± 1 Iba-1 positive cells number, p < 0.05), in the intermediate NTS and in the commissural NTS, respectively. These results suggest a participation of microglia and astrocytes in the chronic inflammation in the NTS that could contribute to the cardiovascular alterations induced by HFD RUBPY IS A NITRIC OXIDE-DONOR THAT INHIBITS PLATELET AGGREGATION TAMY MIDORI BANIN 1 ; ROBERTO SANTANA DA SILVA 1 ; AMRITA AHLUWALIA 2 ; LUSIANE MARIA BENDHACK 1 1.FCFRP/USP, RIBEIRÃO PRETO, SP, BRASIL; 2.WHRI/QMUL, LONDON, REINO UNIDO. The ruthenium complex RuBPY is promising as a new vasodilator because it causes complete relaxation of rat aorta, presents hypotensive effect and it is not cytotoxic. The mechanisms by which, RuBPY causes relaxation were investigated but it is still unknown if it releases nitrite or nitric oxide (NO). Therefore, this study aimed to evaluate if the compound RuBPY releases nitrite or NO to induce vasodilation and if it inhibits platelet aggregation. NO formation in absence of tissue was measured in a sealed glass reaction chamber containing buffer at ph 7.4 and ph 5.0 and sodium nitrite (from 1 to 1000 μm) or RuBPY (1-100 μm), bubbled with nitrogen gas to simulate ischemia. The NO concentration was measured by ozone chemiluminescence and the platelet aggregation was assessed by Light Transmission Aggregation. Human volunteers blood was used and platelet rich plasma was considered equivalent to no aggregation, and platelet poor plasma 100% aggregation. NO production induced by NaNO2 was greater in acid ph with the highest concentration used (10 mm). RuBPY produced higher amount of NO than the 100 µm NaNO2 in both acid and basic ph. The platelet aggregation was accessed in response to stimulation with ADP and collagen. Platelets incubation with 100 µm RuBPY inhibited the platelet aggregation. Taken together, the results showed that RuBPY is an NO donor, that releases NO in a tissue-independent manner and that it is able to inhibit platelet aggregation INCREASE IN PARASYMPATHETIC FUNCTION BY ACETYLCHOLINESTERASE INHIBITION PREVENTS SYMPATHO-EXCITATION AND BAROREFLEX DYSFUNCTION IN HEART FAILURE RATS RENATA M. LATARO; CARLOS ALBERTO AGUIAR SILVA; DANIEL P MARTINS DIAS; FERNANDA MACHADO SANTOS-ALMEIDA; RUBENS FAZAN; HELIO CESAR SALGADO FACULDADE DE MEDICINA DE RIBEIRÃO PRETO, RIBEIRÃO PRETO, SP, BRASIL. Excessive sympatho-excitation and abnormality of baroreflex sensitivity are hallmarks of heart failure (HF). We investigated the effects of the increase of parasympathetic function, by the acetylcholinesterase inhibitor pyridostigmine (PYR), on renal sympathetic nerve activity (RSNA) and baroreflex function in HF rats. HF was induced by myocardial infarction. PYR was administered for 4 weeks, per os. After that the animals underwent surgery to implant stainless steel electrodes around the renal nerve and catheters into the femoral artery and vein. Baroreflex-mediated changes in RSNA were assessed, in conscious state, by the mean AP (MAP) vs. RSNA curve, after intravenous administration of phenylephrine and sodium nitroprusside. HF rats showed higher RSNA that was reduced by PYR (Control:332±31; HF:434±31; HF+PYR:325±13 bursts/min). The range of RSNA (Control:105±1; HF:104±3; HF+PYR:102±1 %max), gain (Control:-1.43±0.1; HF:- 1.65±0.2; HF+PYR:-1.8±0.1%max/mmHg) and the midpoint of the curve [(MAP50); Control: 87±4; HF:91±4; HF+PYR:87±2 mmhg] were not altered in HF rats or after PYR administration. However, both the tachycardic (Control:-2.9±0.3; HF:-1.2±0.1; HF+PYR:-3.7±0.5 bpm/mmhg) and bradycardic (Control:-2.1±0.2; HF:-1.4±0.1; HF+PYR:-1.9±0.3 bpm/mmhg) baroreflex sensitivity were attenuated in HF rats, but improved by PYR. Thus, the long-term administration of PYR reduced RSNA and improved the baroreflex control of HR BAROREFLEX ACTIVATION IN CONSCIOUS RATS MODULATES THE JOINT INFLAMMATORY RESPONSE VIA SYMPATHETIC FUNCTION FERNANDA BROGNARA 1 ; GABRIEL S BASSI 1 ; JACI AIRTON CASTANIA 1 ; JHIMMY TALBOT 1 ; THIAGO M CUNHA 1 ; FERNANDO Q CUNHA 1 ; LUIS ULLOA 2 ; ALEXANDRE KANASHIRO 1 ; DANIEL P MARTINS DIAS 1 ; HELIO CESAR SALGADO 1 1.FACULDADE DE MEDICINA DE RIBEIRÃO PRETO - USP, RIBEIRAO PRETO, SP, BRASIL; 2.NEW JERSEY MEDICAL SCHOOL, NEWARK, ESTADOS UNIDOS. The baroreflex is a critical physiological mechanism controlling cardiovascular function by modulating both the sympathetic and parasympathetic activities. Here, we report that electrical activation of the baroreflex attenuates joint inflammation in experimental arthritis induced by the administration of zymosan into the femorotibial cavity. Baroreflex activation combined with lumbar sympathectomy, adrenalectomy, celiac subdiaphragmatic vagotomy or splenectomy dissected the mechanisms involved in the inflammatory modulation, highlighting the role played by sympathetic inhibition in the attenuation of joint inflammation. From the immunological standpoint, baroreflex activation attenuates neutrophil migration and the synovial levels of inflammatory cytokines including TNF, IL-1β and IL-6, but does not affect the levels of the antiinflammatory cytokine IL-10. The anti-inflammatory effects of the baroreflex system are not mediated by IL-10, the vagus nerve, adrenal glands or the spleen, but by the inhibition of the sympathetic drive to the knee. These results reveal a novel physiological neuronal network controlling peripheral local inflammation BLOQUEIO DE RECEPTORES AT1 CENTRAIS REDUZ A PRESSÃO ARTERIAL EM ANIMAIS ALIMENTADOS COM DIETA HIPERLIPÍDICA JESSICA MATHEUS DE SA; MARIANA DEL ROSSO MELO; GUILHERME F.F. SPERETTA; JOSÉ VANDERLEI MENANI; EDUARDO COLOMBARI; DEBORA SIMÕES ALMEIDA COLOMBARI UNESP - UNIVERSIDADE ESTADUAL PAULISTA, ARARAQUARA, SP, BRASIL. O entendimento da fisiopatologia da hipertensão derivada da obesidade tem sido foco de vários estudos. A obesidade aumenta a atividade do sistema renina-angiotensina. Neste trabalho verificamos o efeito do bloqueio de receptores angiotensinérgicos do subtipo AT1 centrais sobre o aumento da pressão arterial em ratos alimentados com dieta hiperlipídica. Foram utilizados ratos Holtzman ( g) com cânulas de aço inoxidável implantadas no ventrículo lateral (VL) alimentados por 6 semanas com dieta hiperlipídica (DH; 26,4% de gorduras; n = 9) ou dieta padrão (DP; 5,4% de gorduras; n = 5). Após 6 semana de DH ou DP, a artéria femoral foi canulada, e no dia seguinte a pressão arterial média (PAM) e frequência cardíaca (FC) foram registradas em ratos não anestesiados. Losartan (100 μg/1 μl antagonista de receptores AT1) foi injetado no VL. A PAM basal nos animais HD foi maior do que nos animais DP (PAM: 114 ± 3, vs. DP: 103 ± 2 mmhg, p<0,05). Vinte minutos após a injeção de losartan no VL a PAM no grupo DH foi reduzida a níveis semelhantes aos observados no grupo DP (DH: 107 ± 1, vs. DP 105 ± 2 mmhg p > 0,05). Angiotensina II no VL não produziu efeito pressor após o tratamento com losartan, mostrando a eficácia no bloqueio de receptores AT1. Os dados sugerem que receptores AT1 centrais participam do aumento de PAM observado em ratos alimentados com dieta hiperlipídica por 6 semanas. THEME 05 GASTROINTESTINAL PHYSIOLOGY NITRIC OXIDE PARTICIPATES IN THE GASTRIC EMPTYING DECREASE INDUCED ACUTE PHYSICAL EXERCISE IN RATS ANA KAROLINA MARTINS CAVALCANTE 1 ; ROMMULO CELLY LIMA SIQUEIRA 2 ; LORENA BEZERRA MARTINS 1 ; ARMÊNIO AGUIAR DOS SANTOS 2 ; MOISES TOLENTINO BENTO-SILVA 3 1.INTA, SOBRAL, CE, BRASIL; 2.UFC, FORTALEZA, CE, BRASIL; 3.UFPI, TERESINA, PI, BRASIL. Introduction: The acute physical exercise (APE) slows gastric emptying (GE), dependent on the basic acid balance. Objective: To evaluate the role of the pathway of NO on the GE fluid in rats submitted to APE. Methods: Male rats ( g) sedentary (SED) or APE as previously described by (J Appl Physiol 116(9),2014). Divided in: SED, APE-2.5%, pc, APE-5%, pc, Sedentary+L-Name (SED+L-NAME) and APE-5%+L-NAME (APE-5%+L- NAME). We evaluated the levels of corticosterone [cortico], lactate [Lac] and NO. After 18h fasting rats were submitted or not to APE. The rats SED+L-NAME and APE-5%+L-NAME were pre-treated (30min) to (L-NAME 10 mg / kg, ip) 10min after all groups evaluated for GE, according to (J Physiol,131: ,1956). Average data expressed ± EPM compared by ANOVA and Tukey test. Results: We observed a significant decrease in the GE values of APE-2.5% rats and APE-5% compared to the SED(64,7±3,1 and 69,5±1,9 vs 52,1±3,9%). Regarding SED rats, APE-5%rats significantly increased Cortico values(13,6±2,5 vs. 28,5±1,9mg/dL), Lac(1,9±0,1 vs 6,5±0,6mmol/dL), and NO(8,1±0,7 vs 28,0±4,2nmol/mg/protein). The APE-5%+L-NAME group had its inhibition of GE prevented significantly compared to APE-5% (69,5±1,9 vs 55,5±4,6%). We did not observe differences between the SED+L-NAME and APE-5%+L- NAME. Conclusion: The APE decreases GE and increases Lac. This phenomenon seems to be involved in stress as the route of NO, where there was an increase [NO], and prevention of decrease in the GE by pretreatment with L-NAME PRETREATMENT WITH GLUTAMINE PREVENTS LIVER INJURY RESULTING FROM INTESTINAL ISCHEMIA AND REPERFUSION IN RATS NORMA ANAIR POSSA MARRONI 1 ; RENATA HARTMANN 2 ; FRANCIELLI LICKS 2 ; ELIZÂNGELA SCHEMITT 2 ; JOSIELI COLARES 1 ; ROSA MARIA MOURA 1 ; GILMARA ZABOT 3 ; HENRIQUE FILLMANN 3 1.UNIVERSIDADE LUTERANA DO BRASIL, PORTO ALEGRE, RS, BRASIL; 2.UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL; 3.PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL. Background: The intestinal ischemia-reperfusion (I/R) can cause cellular damage to the tissue and distant organs such as the liver occuring the generation of free radicals and the nitric oxide. Aim: Evaluate the effects of glutamine (gln) in an animal model of I/R. Material and methods: Twenty male Wistar rats were divided into four groups: Sham operated (SO), Glutamine+Sham operated (G+SO), ischemia-reperfusion (I/R), Glutamine+ischemia-reperfusion (G+I/R). The rats were subjected to occlusion of the superior mesenteric artery for 30 min and 15 min of reperfusion. The gln (25 mg/kg/day) was administered 24 and 48 h before I/R. Liver injuries were determined for oxidative stress using lipid peroxidation (LPO), levels of nitric oxide metabolites (NO) and the expression of the enzyme inducible nitric oxide synthase (inos) by western blot. Statistical analysis used was ANOVA - Student-Newman-Keuls (mean±sem) significant at p<0.05. Results: Animals treated with gln showed a significant reduced the LPO levels (SO: 0.16±0.01, G+SO: 0.20±0.02, I/R: 0.45±0.03; G+I/R: 0.24±0.02) compared to animals of I/R group. The NO levels showed a decrease in the treated group (SO: 5.4±0.8; G+SO: 6.9±0.8; I/R: 15.6±2.7; G+I/R: 6.1±1.1) and also to reduced expression of inos (SO: 1.2±0.3; G+SO: 0.9±0.30; I/R: 2.5±0.10; G+I/R: 1.8±0.1) compared to I/R group. Conclusion: These results suggest that pretreatment with gln improves liver injury after intestinal I/R injury in rats N-ACETYLCYSTEINE MODULATES ANGIOGENESIS, VASODILATION AND DNA DAMAGE IN STOMACH OF PORTAL HYPERTENSIVE RATS NORMA ANAIR POSSA MARRONI 1 ; FRANCIELLI LICKS 2 ; RENATA HARTMANN 2 ; ELIZÂNGELA SCHEMITT 2 ; CAMILA MARQUES 3 ; JOSIELI COLARES 1 ; MARIANA SOARES 2 ; JULIANA SILVA 1 1.UNIVERSIDADE LUTERANA DO BRASIL, PORTO ALEGRE, RS, BRASIL; 2.UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL; 3.INSTITUTO DE TECNOLOGIA DO PARANÁ, CURITIBA, PR, BRASIL. Background and Aims: Portal Hypertension is associated with the development of a collateral circulation due to increase on portal pressure. Our aim was evaluate the antioxidant effect of N- Acetylcysteine (NAC) on the stomach of portal hypertensive rats. Methods: Animals were divided in four groups (n=6): Sham-operated (SO), SO+NAC, Partial portal vein ligation (PPVL) and PPVL+NAC. NAC (10 mg/kg ip) was administered daily for 7 days starting 8 days after surgery. We performed evaluation of portal pressure, expression of endothelial nitric oxide synthase (enos), 10

11 vascular endothelial growth factor (VEGF) and nitrotyrosine (NTT) by immunohistochemistry and Western blot, and assessed DNA damage in blood sample by comet assay. Variance analysis (ANOVA) and Student-Newman Keuls were used for multiple analysis, and the level of significance was 5% (P < 0.05). For comet assay, we used the Kolmogorov -Smirnov test. Results: The PPVL group showed an increase in portal pressure, enos, VEGF and NTT expression. NAC was able to decrease the values of portal pressure and all the proteins evaluated. Furthermore, NAC was able to modulate DNA damage in PPVL+NAC animals. Conclusions: In conclusion, NAC was able to minimize gastric vasodilation, evaluated by enos, angiogenisis, evaluated by VEGF, oxidative stress evaluated by NTT and protected gastric mucosa also from DNA damage. We suggest NAC is able to protect stomach from the alterations developed by the PPVL procedure MELATONIN ATTENUATING OXIDATIVE DAMAGE IN HEPATIC CARCINOGENESIS INDUCED BY DIETHYLNITROSAMINE IN RATS ANDREA CRISTIANE JANZ MOREIRA UFRGS/HCPA, PORTO ALEGRE, RS, BRASIL. This study developed HCC using diethylnitrosamine (DEN) in rats and proposed the treatment with melatonin (MLT). We evaluated oxidative stress in lipids, protein and DNA, comet assay liver (CA), biochemical and histological analysis. Material and Methods: 28 male Wistar rats, 145g were divided into: (I)Control, (II)HCC DEN 50mg/kg ip, (III)MLT5-DEN 50mg/kg ip+melatonin at 5th week (IV)MLT12-DEN 50mg/kg ip+melatonin at 12th week. MLT (20mg/L) was administrated in water. Blood was collected to evaluate transaminases (AST and ALT), gamma glutamyl transferase (GGT) and alkaline phosphatase (AP) (U/L). Liver samples were removed for CA and histological analysis. Statistical analysis:tuckey test with p<0,05. Results: MLT prolongs the survival in III and IV groups vs II group (p=.0001). MLT reduced damage (DI) and frequency (DF) index in DNA (DI:I=95+27; II=339+56; III=259+42; IV=119+17; DF: I=74+17; II=99+2; III=92+5; IV=60+5). AST(I)107+7 (II) (III) (IV)143+27; ALT(I)54±15 (II) (III)102+36; (IV)=95+18 GGT (I)1+0.9 (II)85+45 (III)43+33 (IV)25+14; AP(I) (II) (III) (IV)212+46; group II showed significant increase compared to groups I, III and IV. Liver histology of II group was characterized by pseudoacinar and trabecular growth pattern. We concluded that chronic and intermittent exposure model to carcinogenic agents develops HCC in rats. Melatonin improved hepatic function and reduced damage on DNA, resulting an increase in OS in rats with HCC. THEME 06 CELULAR PHYSIOLOGY EVALUATION OF OXIDATIVE STRESS INDUCTION BY PUTRESCINE IN LIVER OF WISTAR RATS LETÍCIA CRISTINA GONÇALVES 1 ; LETÍCIA DE SOUSA FRANCO 1 ; GUILHERME ANDRADE ALVES 1 ; RAÍSSA FERRAZ CURTOLO 1 ; FRANCO DANI CAMPOS PEREIRA 2 ; MARIA APARECIDA MARIN-MORALES 2 ; GRASIELA DIAS DE CAMPOS SEVERI-AGUIAR 1 1.FHO- UNIARARARAS, RIO CLARO, SP, BRASIL; 2.UNESP, RIO CLARO, SP, BRASIL. The putrescine acts as an intracellular messenger and is involved in homeostatic mechanisms. When in imbalance is able to induce some diseases like cancer. In order to assess the ability of putrescine at inducing changes in levels of oxidative stress, 24 Wistar rats were exposed by gavage for 56 consecutive days at three different concentrations (T mg/kg; T mg/kg and T mg/kg), and the results were compared with the control group (drinking water). The sulfhydryl groups (-SH), the level of lipid peroxidation (TBARS/MDA), glutathione (GSH) and the activity of catalase (CAT), superoxide dismutase (SOD) and glutathione-s-transferase (GST) were quantification. The statistical analysys were submitted to the tests t and pos hoc, Man Whitney test (p> 0.05). In all groups exhibited a significant decrease was observed for GST (Co ± 0.004; T ± 0.003; T ± 0.001; T ± 0.005) and increase in TBARS (Co ± 27.93; T ± 48.30; T ± 21.91; T ± 23.09). In T1 and T3 groups, levels of SOD (Co ± 0.022; T ± 0.067; T ± 0.074; T ± 0.027) decreased significantly and GSH increased significantly (Co ± 0.002; T ± 0.005; T ± 0.005; T ± 0.005). However, not observed significant changes for the sulfhydryl groups (- SH) as well as to the activity of the enzyme CAT. These results show that putrescine caused lipid peroxidation, and reduced levels of antioxidant enzymes, featuring induction of hepatic oxidative stress THE EFFECT OF STEVIOSIDE ON THE TRANSPORT OF OLEIC ACID IN RAT ADIPOCYTES SUELLEN HARUMI OKUMA; MARIA IDA BONINI RAVANELLI; SÍLVIO CLÁUDIO DA COSTA; CECÍLIA EDNA MAREZE DA COSTA; KELLEN BRUNALDI UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGÁ, PR, BRASIL. This study aimed to evaluate the effect of stevia glycoside stevioside (Stv) on the transport of oleic acid (OA) in isolated adipocytes of the epididymal fat of rats obtained by collagenase digestion. The fluorescent ph probe BCECF was trapped in the cytoplasm and measurements performed with a fluorimeter. Adipocytes were initially incubated with Stv (10 mm) for 4 min. Stv did not affect BCECF fluorescence as it has no ionizable groups. The subsequent addition of OA (1.3 mm) to cells caused a fast drop in fluorescence (t1/2 < 2 sec), which corresponded to the release of H + by OA that had reached the inner leaflet of the plasma membrane. The kinetic of BCECF fluorescence change reflected the combined steps of OA binding and diffusion across the membrane. Addition of OA to cells in the absence of Stv (control experiment) produced similar reduction in BCECF fluorescence. However, none of those data informed about Stv partitioning into the lipid bilayer. Therefore, the Stv analog steviolbioside (Stvbio), which has a carboxyl group, was tested. Stvbio (1.6 mm) caused a fast drop in BCECF fluorescence (t1/2 < 2s), which reflected the binding and diffusion of Stvbio across the membrane, with the delivery of H + to the cytoplasm. We concluded that the Stv did not affect the transport of OA in isolated rat adipocytes. Stv probably partitioned into the lipid bilayer as inferred from the data with Stvbio CAN TEMPERATURE SYNCHRONIZE PERIPHERAL CLOCKS OF ECTOTHERMIC ANIMALS? MARCOS RODRIGO JERONIMO DA COSTA; BRUNO CÉSAR RIBEIRO RAMOS; ANA MARIA DE LAURO CASTRUCCI INSTITUTO DE BIOCIÊNCIAS - USP, SAO PAULO, SP, BRASIL. Thermo-sensation allowed the animals to monitor environmental temperature changes, in order to prevent harmful effects on vital physiological processes. Temperature is a major synchronizing agent of peripheral clocks, through the regulation of clock genes expression. In this aspect, thermo-trp (transient receptor potential) channels are of upmost importance. Using quantitative PCR we demonstrated the expression of the following trp genes in the cell line ZEM-2S of the teleost Danio rerio: trpv1, trpc6, trpa1a, trpa1b, trpm2, trpv4, trpm4a, trpm4b/c and trpm5. To analyze the participation of TRP channels in the modulation of clock genes by temperature, pulses of 2.5 and 5 o C above the maintenance temperature (28 o C) were applied to ZEM-2S cells kept in constant darkness (DD). Our results indicate that the expression of per1, per2, cry1a and cry1b was not affected by 2.5 o C pulse, whereas the 5 o C pulse increased the expression of per2 and cry1a, same clock genes reported to be induced by light. Accordingly, it has been established that TrpV1 is essential for Danio rerio to detect temperatures above 32 o C, what may explain why the increase of just 2.5 o C was ineffective. We are presently investigating the nature of the TRP channel involved in the response of clock genes to 5 o C pulse. THEME 07 COMPARATIVE PHYSIOLOGY PULMONARY MECHANICS AND THE WORK OF BREATHING IN CAIMAN YACARE MICHELLE REICHERT; WILLIAM K MILSOM UNIVERSITY OF BRITISH COLUMBIA, VANCOUVER, CANADA. The pulmonary mechanics and work of breathing of an animal reflect the structure and complexity of the lungs and the chest cavity that surrounds the lungs. Within Class Reptilia, there is large variation in body wall architecture and lung structure, ranging from simple (snake) to complex (crocodilian). We measured static and dynamic pulmonary mechanics in the Yacare caiman (Caiman yacare) and found that total system mechanics primarily reflected the mechanics of the chest wall, but not to the same degree as other reptiles. The lungs of the caiman were complex and less compliant than other reptiles and also contributed significantly to overall stiffness. While total system compliance decreased with increasing breathing frequency, it did not decrease with increasing tidal volume, which was surprising given the heavily keratinized nature of the chest wall. The work of breathing, however, increased with both tidal volume and breathing frequency and the majority of the work performed during breathing was to overcome the elastic forces of the chest wall resisting inflation. As tidal volume and breathing frequency increased, however, the work performed to overcome resistive forces in the airways increased proportionately. While the pulmonary mechanics of the caiman more closely resembled that of other reptiles than that of mammals overall, they begin to take on the characteristics of the mammalian respiratory system EFFECTS OF POSITIONS ON MECHANICS OF VENTILATION IN PHRYNOPS GEOFFROANUS PEDRO HENRIQUE TREVIZAN BAU; WILFRIED KLEIN; TÁBATA ELISE FERREIRA CORDEIRO UNIVERSIDADE DE SÃO PAULO, RIBEIRAO PRETO, SP, BRASIL. The reptilian respiratory system shows great diversity in lung morphology and respiratory mechanisms, which are strongly influenced by morphological differences. Despite sharing respiratory system characteristics with other reptiles, testudines have a unique trait: the ribs are attached to the shell, which could influence breathing mechanics. The aim of this study was to measure, for the first time, the static and dynamic compliance of the intact respiratory system (CT) in P. geoffroanus (n=4) in 4 positions (supine, prone, legs and head retracted into the shell, and submerged), as well as in isolated lungs (CL). Measurements of static compliance were performed by stepwise injection/removal of known air volumes. Dynamic compliance was determined by pump ventilation of the respiratory system at known volumes and frequencies and measuring the resulting pressure changes. Static CL was greater when compared to static CT (43.6±7.1 ml.cmh2o -1.kg -1 ). Comparing submerged to other positions, the values were significantly greater (22.1±1.1 ml.cmh2o -1.kg -1 ) than prone (7.7±1.0 ml.cmh2o -1.kg -1 ), and legs and head retracted (6.6±0.7 ml.cmh2o -1.kg -1 ) and the Residual lung volume/maximum lung volume was lower (20.7±3.2%) because of hydrostatic pressure. This relationship could explain the greater inspiratory reserve and compliance of respiratory system. This result is being confirmed in dynamic compliance experiments where CT tend to be equal in high frequencies between positions but differing at low frequencies STATIC AND DYNAMIC BREATHING MECHANICS IN PODOCNEMIS UNIFILIS TABATA ELISE FERREIRA CORDEIRO; WILFRIED KLEIN; PEDRO HENRIQUE TREVIZAN BAU USP, RIBEIRÃO PRETO, SP, BRASIL; ;. The relationship between pressure and volume of a respiratory system can be described by its compliance (C). In reptiles, increase in the complexity of lungs leads to increased C. Therefore, this study aims to understand the influence of the elastic forces of the respiratory system as well as the lungs in Podocnemis unifilis (N = 5) Static and dynamic C of the intact respiratory system (CT) were determined with animals in supine and prone positions, with their legs retracted into the carapace and while floating in water. Isolated lungs also had their static and dynamic C determined (CL). Resting lung volume (VRL) in animals under the water was lower values (43.6 ± 10) whereas in maximum volume (VML) at the same position was the highest between the different conditions (221.9 ± 7.1). These results can be explained by hydrostatic pressure exerted over the lungs and led to lower ratio of VRL/ VML. Static CT was greatest in submerged animals (CT = 41.3 ± 7.8 ml.cmh2o-1.kg-1) and animals with restrained limbs showed were least complacent respiratory system (CT = 19.2 ± 5.8 ml.cmh2o-1.kg-1), due to the rigid shell limiting lung expansion. Dynamic compliance decreased with increasing frequency in all different position and was not significantly affected by variations in volume. This work was the first that investigated CT and CL in Podocnemis unifilis and with positions observed in nature VARIAÇÔES SAZONAIS NO METABOLISMO DO SIRI CALLINECTES SAPIDUS NA LAGOA DE TRAMANDAÍ (IMBÉ/RS) JORGE FELIPE ARGENTA MODEL; JORDANA TRES SANTOS; ANAPAULA SOMMER VINAGRE; MÁRCIA TRAPP UFRGS, PORTO ALEGRE, RS, BRASIL. O objetivo do trabalho foi verificar as alterações metabólicas sazonais na população de C. sapidus da Lagoa de Tramandaí/RS. Os animais foram coletados (março de 2013 a dezembro de 2014) na Lagoa de Tramandaí/RS, permaneceram em aquários com condições controladas e foram alimentados diariamente com lulas por 15 dias. Os siris foram crioanestesiados para a coleta de hemolinfa e de tecidos (hepatopâncreas, músculos da mandíbula e do quelípede, brânquias anteriores e posteriores e coração). As concentrações hemolinfáticas de glicose, triglicerídeos, colesterol e proteínas totais foram determinadas usando kits comerciais e expressas em mg/dl. Após hidrólise ácida, o glicogênio nos tecidos foi determinado e expresso como mg /g de tecido. Na hemolinfa, as concentrações de triglicerídeos aumentaram do verão (12,9 ± 2,2) em relação ao outono (21,5 ± 2,2); os níveis de colesterol aumentaram do verão (9,8 ± 1,5) comparado ao outono (26,6 ± 2,5) (p<0,05); a concentração de proteínas reduziu do outono (4,1 ± 0,3) comparado ao inverno (2,1 ± 0,8) (p<0,05), enquanto a glicemia não variou. Os níveis de glicogênio nos músculos da mandíbula (0,5 ± 0,1), do quelípede (0,08 ± 0,02) e do coração (1,79 ± 0,4) reduziram no verão (p<0,05). Nos demais tecidos não houve diferença significativa. Esses resultados demonstram a presença de variações sazonais no metabolismo de C. sapidus EFEITOS METABÓLICOS DA ADRENALINA NO CARANGUEJO NEOHELICE GRANULATA ANAPAULA SOMMER VINAGRE; JORGE FELIPE ARGENTA MODEL UFRGS, PORTO ALEGRE, RS, BRASIL. O objetivo foi investigar os efeitos da adrenalina sobre o metabolismo do caranguejo Neohelice granulata. Caranguejos machos foram mantidos em aquários, em condições controladas, e alimentados com carne bovina (dieta RP) ou arroz cozido (dieta RC) durante 15 dias. Para a curva de tempo, os animais foram crioanestesiados e amostras de hepatopâncreas, brânquias anteriores (BA) e brânquias posteriores (BP) foram incubadas, a 25ºC com Ringer ou adrenalina 2,5 mm e ácido ascórbico (AA) 0,1 M por 15, 30 ou 60 min. Após a incubação foi determinada a glicose dos meios. Para a curva de dose, os tecidos foram incubados com adrenalina nas doses 10-7M, 10-6M, 10-5M, 10-4M e 10-3M e AA por 30min. Após a incubação foi determinada a concentração de glicose e glicerol dos meios e a de glicogênio dos tecidos. Tanto nas BP como nas BA de animais RC incubados com Ringer, a liberação de glicose para o meio foi máxima aos 30 min, enquanto no hepatopâncreas não foram verificadas alterações significativas. Os valores de glicogênio e de glicerol não sofreram alterações significativas em relação às doses de adrenalina. Os valores de glicose nos meios diminuíram na presença de adrenalina 10-3M no hepatopâncreas e 10-6M nas BP. Esses resultados sugerem uma ação da adrenalina sobre a oxidação da glicose, o que ainda precisa ser investigado. THEME 08 RESPIRATORY PHYSIOLOGY PURINERGIC RECEPTORS BLOCKADE IN THE RETROTRAPEZOID NUCLEUS ATTENUATES THE RESPIRATORY RESPONSE TO CHEMOREFLEX IN AWAKE RATS BARBARA FALQUETTO BARNA; ANA CAROLINA T TAKAKURA; THIAGO S MOREIRA UNIVERSITY OF SÃO PAULO, SAO PAULO, SP, BRASIL. Central and peripheral chemoreceptors sense changes in CO2/H+ and/or O2 and communicate this information to cardiorespiratory centers to regulate breathing and sympathetic outflow to ensure adequate ventilation-perfusion matching in tissues. Despite the importance of this reflex, the neurotransmitter basis for integration of this information in the brainstem is unclear. Therefore, our main goal was to elucidate whether purinergic signaling contributes to central and peripheral chemoreceptor activation in the RTN in unanesthetized rats. Male Wistar rats with bilateral stainless steel cannula implanted into the RTN were used. Bilateral injection of PPADS (a P2 receptor blocker - 5 mm nl) into the RTN attenuated the excitatory effect of ATP on minute ventilation (Ve: 839 ± 115, vs. ATP 3808 ± 1047 ml/kg/min) and the increase in Ve produced by hypercapnia (7% CO2) ( ± 89.1, vs. saline: ± ml/kg/min) and hypoxia (8% O2) (650.9 ± 151, vs. saline: ± ml/kg/min). The bilateral blockade of the P2Y1 receptors (MRS M nl) into the RTN region did not change the excitatory effect of ATP nor the increase in Ve produced by hypercapnia or hypoxia in conscious unrestrained rats. 11

12 These results support that i) the cardiorespiratory effects elicited by ATP are depended on P2 receptors but not P2Y1; ii) the hypercapnia and hypoxia ventilatory responses are mediated by purinergic signaling in conscious unrestrained rats THE PRESENCE OF ACTIVE EXPIRATION IN RAT IN VIVO AND IN VITRO PREPARATIONS SARAH JENKIN UNIVERSITY OF BRITISH COLUMBIA, VANCOUVER, CANADA. Mammalian expiration is typically passive at rest, yet when respiratory drive increases expiration becomes active (AE). This study compares the presence of active expiration in rats using in vivo and in vitro preparations. AE can be elicited in vivo when unanesthetized rats are under high respiratory drive (8% inspired CO2 or higher). AE is elicited more easily when unanesthetized, post-vagotomy (6% inspired CO2). AE cannot be elicited in vivo under anesthetic with or without the vagi intact. These data suggest that vagal feedback and anesthesia (urethane) inhibit active expiration, with the effects of the anesthetic overriding the effects of vagotomy. AE cannot be elicited from the in vitro brainstem-spinal cord preparation (with or without the pons) even though there is no vagal feedback. This is most likely due to the inability to provide sufficient respiratory drive to the preparation. Upcoming research will investigate the role of the vagus nerve, the pons and hypercapnia on the appearance of AE using the in situ working heart-brainstem preparation EFFECTS OF TYROSINE KINASE INHIBITOR DASATINIB IN PULMONARY AND EXTRAPULMONARY ACUTE LUNG INJURY AND HUMAN NEUTROPHILS AMANDA DE LACERDA CLEVELARIO; JOHNATHAS DUTRA SILVA; GISELE PENA DE OLIVEIRA; GISELE PADILHA; DEBORA GONÇALVES XISTO; PATRICIA RIEKEN MACEDO ROCCO UFRJ, RIO DE JANEIRO, RJ, BRASIL. RATIONALE: Evidence suggests that tyrosine-kinase inhibitors may attenuate lung inflammation and fibrosis in acute respiratory distress syndrome (ARDS). Since the pathophysiology of ARDS differs depending on the type of primary insult, we hypothesized that dasatinib, a tyrosine-kinase inhibitor, might act differently depending on ARDS etiology and dose. METHODS: 64 C57BL/6 mice were randomly assigned across eight groups. In the Cp group, saline solution was instilled intratracheally (i.e.) while Cexp received saline intraperitoneally (i.p.). ARDS mice received Escherichia coli lipopolysaccharide i.t. (ARDSp) or i.p. (ARDSexp). At 24h, animals received dasatinib (10, 20 or 30 mg/kg) or vehicle (1% dimethyl sulfoxide) by oral gavage. After 24h, lung mechanics and histology were assessed. Mortality and lung edema were evaluated in other groups of animals treated with dasatinib 10 mg/kg twice daily by oral gavage for 7 weeks. RESULTS: Static lung elastance and pulmonary damage increased similarly after ARDS induction and were reduced by dasatinib 30 mg/kg, with no significant changes with 10 and 20 mg/kg regardless of ARDS model. Dasatinib decreased mortality, body weight and pulmonary edema in ARDSp compared to ARDSexp. CONCLUSION: Dasatinib improved lung morphofunction regardless of ARDS etiology, but beneficial effects were observed only with a higher dose. However, chronic use of low-dose dasatinib reduced mortality rate in pulmonary ARDS THE ROLE OF METABOTROPIC GLUTAMATE RECEPTORS IN THE LATERAL HYPOTHALAMUS AND PERIFORNICAL AREA IN VENTILATORY RESPONSE TO HYPERCAPNIA IN RATS DURING WAKEFULNESS LAISA TAIS CABRAL RODRIGUES 1 ; JOSÉ DE ANCHIETA DE CASTRO HORTA JÚNIOR 2 ; LUCIANE HELENA GARGAGLIONI BATALHAO 3 ; MIRELA BARROS DIAS 1 1.UNESP- UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE MESQUITA FILHO - DEPARTAMENTO DE FISIOLOGIA, BOTUCATU, SP, BRASIL; 2.UNESP - UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE MESQUITA FILHO, DEPARTAMENTO DE ANATOMIA, BOTUCATU, SP, BRASIL; 3.UNESP - FCAV - DEPARTAMENTO DE MORFOLOGIA E FISIOLOGIA ANIMAL, JABOTICABAL, SP, BRASIL. Introduction: Evidence suggests there is a diurnal variation of central chemoreflex, and it has been suggested that orexinergic neurons of lateral hypothalamus and area perifornical (LH/PFA) have a key role in the modulation of the central chemosensitivity in a vigilance-state manner, but the mechanisms involved have not been elucidated so far. Objective: To evaluate the importance of metabotropic glutamate receptors, located in the LH/PFA, in the hypercapnic ventilatory response in unanesthetized rats, during wakefulness and sleep. Methods: We measured pulmonary ventilation (VE), and body temperature (Tb) together with electroencephalogram (EEG) and electromyogram (EMG) before and after microinjecting alpha-methyl-4-carboxyphenylglycine (MCPG; 100mM) or vehicle (acsf) into the LH/PFA of male Wistar rats, followed by 40 min of hypercapnia exposure (7% CO₂). Nissl staining procedure was used to evaluate, based on the cytoarchitecture of the LH/PFA region, the location of injections. Results: MCPG injected into LH/PFA decreased the ventilatory response to hypercapnia due to a lower respiratory frequency in awake rats, compared with the control group. Conclusion: These data suggest that the glutamate acting on metabotropic receptors in the LH/PFA contributes to the hyperventilation induced by hypercapnia during wakefulness GAP JUNCTIONS IN THE ROSTRAL MEDULLARY RAPHE MODULATE THE HYPERCAPNIC VENTILATORY RESPONSE IN UNANESTHETIZED RATS LAISA TAIS CABRAL RODRIGUES 1 ; RAIMUNDO DA SILVA SOARES JÚNIOR 1 ; FELIPE FIORIN ZAMAI 1 ; JOSÉ DE ANCHIETA DE CASTRO HORTA JÚNIOR 2 ; LUCIANE HELENA GARGAGLIONI BATALHAO 3 ; MIRELA BARROS DIAS 1 1.UNESP - UNIVERSIDADE ESTADUAL PAULISTA JULIO DE MESQUITA FILHO, DEPARTAMENTO DE FISIOLOGIA, BOTUCATU, SP, BRASIL; 2.UNESP - UNIVERSIDADE ESTADUAL PAULISTA JULIO DE MESQUITA FILHO, DEPARTAMENTO DE ANATOMIA, BOTUCATU, SP, BRASIL; 3.UNESP - FCAV, DEPARTAMENTO DE MORFOLOGIA E FISIOLOGIA ANIMAL, JABOTICABAL, SP, BRASIL. Introduction: It has been demonstrated that Medullary Raphe (MR) plays an important role in central chemoreception. Gap junction proteins are expressed in neurons of putative CO2- chemosensitive brainstem regions, including the MR, but it is not known whether gap junctions, located specifically in the rostral MR, contributes to the ventilatory response to hypercapnia. Objective: The present study was designed to investigate the involvement of gap junctions in the CO2 ventilatory response in unanesthetized adult rats. Methods: Pulmonary ventilation (measured by body plethysmography) and body temperature (Tb) were determined before and after injection of carbenoxolone (CARB; 3mM/100nL) or vehicle (sterile 0,9% saline) into the rostral MR of Wistar rats. These measurements were made during 30 min in normocapnic conditions followed by 40 min of 7% CO2 exposure. Nissl staining procedure was used to evaluate, based on the cytoarchitecture of the MR region, the location of injections. Results: Gap junction blockade in MR neurons augmented the ventilatory response to hypercapnia ( ±285,57mL/kg/min for the control group and ±194.91mL/kg/min for 3mM CARB, P<0.01) due to an increased tidal volume. Conclusion: These results suggest that gap junctions in the rostral MR exert an inhibitory modulation of the hypercapnic ventilatory response of adult rats ACUTE HYPOXIA INCREASES PROINFLAMMATORY MARKERS IN CENTRAL AUTONOMIC NUCLEI TALITA DE MELO E SILVA; REINALDO CORREIA SILVA; LAIALI JURDI CHAAR; VAGNER ROBERTO ANTUNES; NIELS OLSEN CÂMARA; ANA CAROLINA T TAKAKURA; THIAGO S MOREIRA UNIVERSITY OF SÃO PAULO, SAO PAULO, SP, BRASIL; ; ; ; ; ;. Prolonged and continuous exposure of mammals to a low oxygen environment (chronic hypoxia) elicits remarkable physiological adjustments. Among them are altered gene expression, increased peripheral chemosensitivity, enhanced respiratory drive and sympathoexcitation. The current study examines the hypothesis that acute hypoxia initiates an immune response elicited by an increased expression of inflammatory markers. Male Wistar rats ( g; 6-8/group, CEUA: 163/2012) were subjected to acute hypoxia (AH: 8% O2) or normoxia (N: 21% O2) for 3 hours. AH increased the proinflammatory cytokine IL-6 in the heart (90.8 ± 20 vs. N: 1.8 ± 0.7), lung (15.9±3.1 vs. N: 3.3±1.4), kidney (12.1±3.2 vs. N: 1.2±0.2) and liver (2.9±0.5 vs. N: 1.0±0.06) as well as in brain areas such as the rostral ventrolateral medulla (RVLM) (2.43±0.29 vs. N: 1.0±0.12) and paraventricular nucleus of the hypothalamus (PVH) (1.8±0.2 vs. N: 0.8±0.1). AH also increased IL-1 β within the RVLM (3.4±0.3 vs. N: 0.8±0.06) and PVH (1.69±0.3 vs. N: 0.9±0.1), TNF within the RVLM (2.37±0.7 vs. N: 0.7 ± 0.04), CD86 within the PVH (1.43±0.1 vs. N: 0.9±0.06), LY6C within the RVLM (8.6±2.6 vs. N: 1.0±0.26) and MMP9 within the RVLM (2.3±0.5 vs. N: 0.82±0.09) and PVH (1.26±0.3 vs. N: 0.5±0.05). Taking together these results suggest that inflammatory mechanisms may be involved in the response to chemoreceptors activation during AH ACUTE HYPOXIA ACTIVATES HYPOTHALAMIC PARAVENTRICULAR NUCLEUS- PROJECTING CATECHOLAMINERGIC NEURONS IN THE C1 REGION TALITA DE MELO E SILVA; ANA CAROLINA T TAKAKURA; THIAGO S MOREIRA UNIVERSITY OF SAO PAULO, SAO PAULO, SP, BRASIL. C1 cells reside in the rostral and intermediate portions of the ventrolateral medulla (RVLM) and can be activated by hypoxia. These neurons regulate the hypothalamic pituitary axis via direct projections to the hypothalamic paraventricular nucleus (PVH) and regulate the autonomic nervous system via projections to sympathetic and parasympathetic preganglionic neurons. Based on the various effects attributed to the C1 cells and what is currently known of their synaptic inputs, our hypothesis is that acute hypoxia (AH) activates PVH projecting catecholaminergic neurons in the RVLM. Anterograde tracer, Phaseolus vulgaris leucoagglutinin (PHA-L) was unilaterally injected into the RVLM and a retrograde tracer Cholera toxin B (CTB) was unilaterally injected into the PVH region. After ten days, male Wistar rats that received CTB injection into the PVH were subjected to AH (8% O2, balanced with N2) or normoxia (21% O2) for 3 hours. Acute hypoxia significantly increased Fos immunoreactivity in the C1 region (68.5 ± 2.0), and half of the RVLM cells activated are catecholaminergic (35.5 ± 2.3). We observed that (30 ± 4%) of the PVH projecting RVLM cells that were activated by AH were also C1 cells. The presence of varicosities containing PHA-L in PVH region was also observed. The present results suggest that catecholaminergic C1-PVH projection is hypoxia sensitive and the pathway between these two important brain areas can certainly be one more piece in the complex puzzle of neural control of autonomic regulation during hypoxia HYPERCAPNIA-SENSITIVE CHEMORECEPTORS NEURONS OF RETROTRAPEZOID NUCLEUS INNERVATE THE KOLLIKER-FUSE REGION IN RATS JOSIANE DO NASCIMENTO SILVA; TALITA DE MELO E SILVA; ROSÉLIA DOS SANTOS DAMASCENO; ANA CAROLINA T TAKAKURA; THIAGO S MOREIRA USP, SÃO PAULO, SP, BRASIL. The Kölliker-Fuse (KF) region, located in the dorsolateral pons, receives several projections from brainstem areas involved in respiratory regulation, including the chemoreceptors neurons within the retrotrapezoid nucleus (RTN). We hypothesized that the KF region could contribute to central respiratory chemoreflex activation. To test this hypothesis, we used immunohistochemistry experiments in male Wistar rats ( g, CEUA: 008/2012). After iontophoretic injection of the anterograde tracer biotinylated dextran amine (BDA) in RTN, most (35 ± 6%) BDA-labeled terminals in the KF region were immunoreactive for vesicular glutamate transporter-2 (VGLUT2). Awake rats were exposed to hypercapnia one week after iontophoretic injection of the retrograde tracer FluoroGold (FG) into the KF. Hypercapnia-activated neurons were identified by the presence of Fos-immunoreactive (Fos-ir) nuclei. Fos-ir RTN neurons retrogradely labeled with FG were detected only in hypercapnia-treated rats. In RTN, 84 ± 6% of Fos-ir neurons with projections to RTN (FG-immunoreactive) were not tyrosine hydroxylase. In conclusion, the RTN-KF neuronal connection is probably one of several brainstem pathways through which the RTN neurons activate breathing THERAPEUTIC EFFECTS OF DENDRIMER-METHYPREDNISOLONE CONJUGATE IN AN EXPERIMENTAL MODEL OF ACUTE LUNG INJURY ROBERTA ESPIRITO SANTO CORREIA; JOHNATHAS DUTRA SILVA; SABRINA V MARTINI; PATRICIA RIEKEN MACEDO ROCCO; MARCELO MARCOS MORALES UFRJ, RIO DE JANEIRO, RJ, BRASIL. This study investigated the effects of dendrimer-methyprednisolone conjugated in an experimental models of acute respiratory distress syndrome (ARDS). In BALB/c mice, ARDS was induced by intratracheal (ARDSp) or intraperitoneal (ARDSexp) injection of E.coli LPS. In control groups, animals received saline (Cp, Cexp). 24 hours after ARDS induction, groups were divided receiving saline (SAL, iv); Methyprednisolone (MP, iv) or intratracheal instilations of dendrimermethyprednisolone conjugated (D-MP). 24 hours after the treatment, survival rate, pulmonary mechanics, lung histology and inflammatory mediators were analyzed. The ARDSexp-SAL group had a survival of 80%, MP or D-MP improved survival in ARDSexp-SAL animais (8%). Lung elastance was increased in ARDS animals, the treatment with MP or D-MP attenuated these functional changes. In ARDS animals, lung histology presented neutrophil infiltration, alveolar collapse, interstitial oedema, and increase in the amount of collagen and elastic fibers in the alveolar septa. Additionally, levels of IL (interleukin)1-b, KC (murine homolog of IL-8) and vascular endothelial growth factor (VEGF) were higher in ARDS groups. MP and D-MP treatment attenuated these changes, however, in ARDSexp-D-MP the treatment significantly reduced the collapsed areas, the neutrophil infiltration and attenuated the increase of cellularity and inflammatory mediators. The treatments improved survival, prevented pulmonary mechanics deterioration, minimized histological changes and inflammatory mediators in these experimental models of ARDS CHARACTERIZATION OF EMPHYSEMA MOUSE MODEL INDUCED BY MULTIPLE INSTILLATIONS OF ELASTASE MILENA VASCONCELLOS DE OLIVEIRA; NAZARETH DE NOVAES ROCHA; GISELE PADILHA; LIGIA DE ALBUQUERQUE MAIA; DEBORA GONÇALVES XISTO; SORAIA CARVALHO ABREU; PATRICIA RIEKEN MACEDO ROCCO IBCCF-UFRJ, RIO DE JANEIRO, RJ, BRASIL. Rationale: Although there are several therapeutic strategies for pulmonary emphysema, none is able to prevent disease progression. This study sought to characterize a model of emphysema induced by multiple instillations of elastase through periodic analyses of inflammation and cardiovascular function. Methods: 48 male C57BL/6 mice were randomly assigned into 2 groups. Emphysema (ELA) animals received 1, 2, 3 or 4 intratracheal instillations of elastase (PPE, 0.2 UI) with a 1-week interval between them. Controls (C) received saline using the same protocol. Results: After instillation 1, the percentage of mononuclear cells in the lung parenchyma increased. After instillation 2, the fraction of hyperinflated alveoli and mean linear intercept increased, and the elastic fiber content of the lung parenchyma was reduced. After instillation 3, percentage of neutrophils in the lung and collagen fiber content in the lung parenchyma and airways increased, and lung static elastance was reduced. After instillation 4, the percentage of macrophages with M1 phenotype in the lung, levels of cytokines and growth factors, and collagen fiber content in blood vessels were increased, and pulmonary arterial hypertension developed. Conclusion: These findings suggest that the initial phase of emphysema is characterized by an inflammatory process, whereas its late phase is marked by impairment of pulmonary and cardiovascular function THERAPEUTIC EFFECTS OF BOSUTINIB, A TYROSINE KINASE INHIBITOR, ON LUNG MORPHOFUNCTION AND INFLAMMATION IN EXPERIMENTAL SILICOSIS PRISCILA DE JESUS CARNEIRO; AMANDA DE LACERDA CLEVELARIO; GISELE PADILHA; FERNANDA FERREIRA CRUZ; NAZARETH DE NOVAES ROCHA; PATRICIA RIEKEN MACEDO ROCCO UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL. Rationale: Silicosis is an occupational lung disease for which no effective treatment exists. We hypothesized that bosutinib, a tyrosine kinase inhibitor, would attenuate pulmonary fibrosis, ameliorate inflammatory responses, and improve lung function in experimental silicosis. For this purpose, we investigated the potential efficacy of bosutinib in the treatment of experimental silica-induced lung fibrosis. Methods: Silicosis was induced in C57BL/6 mice (n=32) by intratracheal administration of silica particles (20 mg/50µl saline); a control group received saline alone. After 15 days, when the disease was established, animals were randomly assigned to receive DMSO or bosutinib (1 mg/kg dose in 0.1 ml of DMSO) by oral gavage, twice daily for 14 days. At day 30, lung mechanics (airway resistance, viscoelastic pressure and static elastance) and morphometry, pulmonary inflammation (total and differential cell count in alveolar septa), and amount of collagen fibers were measured. 12

13 Results: Bosutinib improved lung mechanics (p<0.05) and reduced fractional area of alveolar collapse, size and number of granulomas, amount of collagen fibers, and number of macrophages in alveolar septa and granuloma. Conclusion: In this model of silicosis, bosutinib effectively reduced lung inflammation and fibrosis, thus improving lung mechanics GLUCOCORTICOIDS INFLUENCE IN ACUTE LUNG INJURY IN SEPTIC RATS ERIKA KRISTINA INCERPI GARCIA; ROSELI SONCINI; SILVIA GRACIELA RUGINSK.,., MG, BRASIL. In Acute Respiratory Distress Syndrome (ARDS) and Acute Lung Injury (ALI), a down regulation of systemic inflammation mediated by glucocorticoid receptor (GR) is essential to improve survival. We investigate the dynamic interaction between agonist and inhibitor of glucocorticoids (GC) on corticosterone (CCT) plasma levels and immunoreactive area for GR in rat lung with LPA-induced CLP. We use male Wistar rats ( g) divided into groups Naïve, Sham and CLP (n = 8 / group). The Naïve group did not undergo the procedure or treatment. Sham group had the exposed cecum and was pretreated with saline. The CLP group had bonded and perforated cecum and divided into three groups according to the treatments one hour before surgery: CLP sal (NaCl 0.9% saline i.p.); CLP met (metyrapone 50 mg.kg-1 i.p.); CLP dex (dexamethasone 0.5 mg.kg-1 i.p.). After 24 hours, the CLP sal had increased plasma CCT and no change in immunoreactivity for GR. The CLP met showed reduced levels of CCT, decreased cytoplasmic and total immunoreactivity for GR and increased nuclear, and increased risk of death. The CLP dex showed reduced levels of CCT, increased nuclear immunoreactivity for GR and decreased risk of death. We concluded that there was GR resistance in animals pre-treated with sal; there was increased GR sensitivity in the pretreated with met and dex. However, met did not prevent mortality due to reduced supply of CCT to tissues GLIMEPIRIDE DOES NOT EXHIBIT CHANGES ON RESPIRATORY RESISTANCES IN NON- OBESE MICE: A PRELIMINARY STUDY MELISSA TEIXEIRA GOULART; CECÍLIA DA COSTA DALÉCIO; MAYCON DANIEL DIAS; MARINA DE FÁTIMA VENÂNCIO; ROSELI SONCINI UNIVERSIDADE FEDERAL DE ALFENAS, ALFENAS, MG, BRASIL. Glimepiride is considered a low-potency insulin secretagogue and is used on glycaemic control. Literature points an association between insulin and development/severity of asthma, meanly withlung resistance. We evaluated the glimepiride on airway and tissue resistances, and on collagen and elastic fibers in non-obese mice. Nine Swiss mice (w=45±0.7g) were divided in: control group, treated withsaline (n=4); or glim group,treated withsaline+glimiperide(0.1mg/kg; n=5). All animals received the solutions by gavage during 21 days. The glycaemia was performedand after, the animalswere tracheostomized and ventilated with a ventilator. Airway resistance and tissue damping were investigated after lung aerosolizationof saline and methacholine (100mg/kg). Lungs were collected and prepared for morphometric analysis to collagen and elastic fibers. Glycaemia did not changein any group (134±15mg/dL). Also, we didn t identify changes in the respiratory parameters. Only parenchyma collagen fibers percentage was reduced in the group glim in comparison to control (9.4±1% vs4.3±0.6%, P<0.005). Glimepiride does not show changes in the respiratory function in non-obese mice, however, the collagen fibers were reduced. It is known that there are interactionsof the smooth musclesof the airwaysand thetissuematrix,and we believe that new protocols will run in non-obese and obese mice in order to elucidate some questions of this preliminary study PRE-INSPIRATORY ACTIVITY EVOKED BY RETROTRAPEZOID NUCLEUS (RTN) STIMULATION IS DEPENDENT ON THE PONTINE KÖLLIKER-FUSE (KF) REGION INTEGRITY ELVIS VIANA DE LUCENA 1 ; ANA CAROLINA T TAKAKURA 2 ; THIAGO S MOREIRA 1 1.DEPARTMENT OF PHYSIOLOGY AND BIOPHYSICS, INSTITUTE OF BIOMEDICAL SCIENCE, UNIVERSITY OF SÃO PAULO, SÃO PAULO, SP, BRASIL; 2.DEPARTMENT OF PHARMACOLOGY, INSTITUTE OF BIOMEDICAL SCIENCE, UNIVERSITY OF SÃO PAULO, SÃO PAULO, SP, BRASIL. Pre-inspiratory activity underlies tongue muscle contraction and expansion of the upper airway, causing a decrease in airway resistance. The pontine Kölliker-Fuse nucleus (KF) has established functions in the regulation of upper airway patency. We hypothesized that the KF region could contribute to hypoglossal motor activity to central respiratory chemoreflex activation. To test this hypothesis, we performed electrophysiological experiments in adult urethane-anesthetized and vagotomized male Wistar rats ( g, CEUA: 008/2012). Hypercapnia (10% CO2) increased diaphragm (DiaEMG) and genioglossal (GGEMG) activity and elicited abdominal (AbdEMG) muscle activity. Bilateral injection of muscimol (GABA-A agonist; 2mM) into the KF region eliminated the rise in GGEMG (6 ± 4 vs. saline: 47 ± 9%) and reduced the increase in DiaEMG (25 ± 6 vs. saline: 43 ± 8%) and AbdEMG (213 ± 23 vs. saline: 335 ± 18%) produced by hypercapnia. The blockade of the KF region were able to eliminate the GGEMG (2 ± 2%), but only reduced the DiaEMG (12 ± 2%) and AbdEMG (111 ± 13%) induced by NMDA (1 mm) injection into the RTN. Our data suggest that the KF neurons could integrate and modulate several aspects of breathing during chemoreceptor activation EFFECTS OF DISINHIBITION OF RTN/PFRG NEURONS ON THE TACHYPNEIC RESPONSE INDUCED BY L-GLUTAMATE EDUARDO VIEIRA LEMES; DANIEL BRESEGHELLO ZOCCAL SÃO PAULO STATE UNIVERSITY, ARARAQUARA, SP, BRASIL. The retrotrapezoid nucleus/parafacial respiratory group (RTN/pFRG) is described as an important area for central chemoreception and for the generation of active expiration. Experimental evidence suggests that the RTN/pFRG neurons are under a tonic inhibitory control. In the present study, we tested the possibility that the inhibitory mechanisms of RTN/pFRG may modulate the amplitude of inspiratory response to microinjections of L-glutamate in this region. Using arteriallyperfused decerebrated in situ preparations of juvenile male Holtzman rats (60-70g; n=4), we recorded phrenic nerve (PN) activity and performed unilateral microinjections of L-glutamate (100mM, 50nL) in the RTN/pFRG before and after microinjections of bicuculline and strychnine (50μM, 50 nl). L-Glu microinjections in the RTN/pFRG increased PN frequency (ΔPN freq: 41 ± 9 %; P<0.05), but not amplitude (ΔPN amp: 6 ± 7 %). After RTN/pFRG disinhibition, the magnitude of changes in PN frequency (ΔPN freq: 39 ± 6 %) and amplitude (ΔPN amp: 7 ± 3 %) did not change. Our data indicate that the tachypneic response to L-glutamate microinjections is not influenced by the inhibitory mechanisms of the RTN/pFRG. THEME 09 ENDOCRINE & METABOLISM UROCORTIN 2 ENHANCES SKELETAL MUSCLE MASS AND STIMULATES AMPC/PKA/CREB AND AKT/FOXO1 SIGNALING PATHWAYS IN RODENTS NATALIA LAUTHERBACH ENNES DA SILVA 1 ; DAWIT ALBIEIRO PINHEIRO GONÇALVES 1 ; SILVIA DE PAULA GOMES 2 ; NEUZA MARIA ZANON 1 ; LUIZ CARLOS CARVALHO NAVEGANTES 1 ; ISIS DO CARMO KETTELHUT 3 1.RIBEIRÃO PRETO MEDICAL SCHOOL - UNIVERSITY OF SÃO PAULO/ DEPARTMENT OF PHYSIOLOGY, RIBEIRAO PRETO, SP, BRASIL; 2.RIBEIRÃO PRETO MEDICAL SCHOOL - UNIVERSITY OF SÃO PAULO/ DEPARTMENT OF BIOCHEMISTRY/IMMUNOLOGY, RIBEIRAO PRETO, SP, BRASIL; 3.RIBEIRÃO PRETO MEDICAL SCHOOL - UNIVERSITY OF SÃO PAULO/ DEPARTMENTS OF BIOCHEMISTRY AND PHYSIOLOGY, RIBEIRAO PRETO, SP, BRASIL. Introduction: Recent studies reported that Urocortin 2 (Ucn2) presents anti-atrophic and hypertrophic effects in skeletal muscle of rodents. However, the intracellular mechanisms responsible for these findings remain unclear. This work investigates the role of Ucn2 in the signaling pathways involved in the control of protein metabolism in skeletal muscle. Methods: EDL and soleus skeletal muscles were isolated from normal Wistar rats and C57Bl6 mice and incubated with Ucn2 (10-8 M, M, 10-7 M, M) for 2h to investigate the rates of protein degradation by measuring the tyrosine release. C2C12 myotubes were incubated with Ucn2 (10nM) for 5min, 30min and 3 hours. In order to superexpress this peptide, mouse Ucn2 plasmid was transfected in vivo in tibialis anterior muscle of normal C57Bl6 mice for 7 and 14 days. Then, tibialis anterior and C2C12 were processed to determine the phosphorylation levels of PKA substrates, CREB, Akt, Foxo1 and ERK1/2 by Western Blot. Results: Ucn2 decreased overall proteolysis in vitro in soleus and EDL muscles. The super expression of Ucn2 in vivo induced hypertrophy in tibialis anterior (14 days) and increased the phosphorylation levels of CREB, Akt, Foxo1 and ERK1/2 (7 days). Ucn2 in vitro enhanced phospho-pka substrates in myotubes. Conclusion: These results suggest that the anti-proteolytic and hypertrophic effects of Ucn2 are mediated by the activation of AMPc/PKA/CREB, Akt/Foxo1 and ERK1/2 signaling pathways STRUCTURAL ORGANIZATION OF THE PLACENTA IN LATE PREGNANCY OF RATS FED 50% CALORIC RESTRICTION ROSANA CATISTI; KATIA ESTEFANIA DE CARVALHO; LARISSA RUELLA DE ASSIS; HÉRCULES JONAS REBELATO; MARCELO AUGUSTO MARRETO ESQUISATTO CENTRO UNIVERSITÁRIO HERMÍNIO OMETTO, ARARAS, SP, BRASIL. Placental weight is highly correlated with birth weight and therefore, survival and growth rate of the newborn mammal. Placental growth is maximal in the first half of gestation suggesting that placental size and even function later in gestation may be substantially influenced by food restriction in this time. This study investigated whether gestational caloric restriction alters placenta morphology at end of gestation. Pregnant female Wistar rats were divided in control group (C, n = 6, basal diet) and restricted group fed 50% of the diet consumed by control (R, n = 6). At day 21 of gestation (21 dg), after anestesia, the placenta were collected and weighed. For histological analysis were processed fragments of right horn placenta located in the middle region. It was observed significant decrease in the R group in relation to placenta and fetuses weight. Morphometric analysis of the placenta regions, the junctional and the labyrinth zone did not detect size alterations. However, there was a decrease in the number of giant and glycogen cells. It was observed an increase in placenta glycogen content in R rats. The current data led us to hypothesize that maternal caloric restriction altered the placental size but its structural organization were preserved.study approved by CEUA/UNIARARAS (055/2014) STRUCTURAL ORGANIZATION OF THE LIVER IN LATE PREGNANCY OF RATS FED 50% CALORIC RESTRICTION JOYCE FERNANDA CAVENAGHI RADDI; LARISSA RUELLA DE ASSIS; MARCELO AUGUSTO MARRETO ESQUISATTO; HÉRCULES JONAS REBELATO; RENATA BARBIERI; ROSANA CATISTI CENTRO UNIVERSITÁRIO HERMÍNIO OMETTO, ARARAS, SP, BRASIL. Pregnancy is a state of dynamic changes in metabolism and nutrient utilization. The liver plays an essential role in maternal metabolic adaptation during late pregnancy. Glucose is metabolized to supply energy that is used to promote fetal growth and development and that is converted to fetal liver glycogen to ensure neonatal survival. Despite this well-described metabolic plasticity, the morphology changes underlying the hepatic adaptation to pregnancy remain unclear. The aim of this study was to investigate whether 50% caloric restriction diet intake during pregnancy alters structural organization in female rat liver at day 21 of gestation (21 dg). Pregnant female Wistar rats were divided in control group (C, n = 6, basal diet) and restricted group fed 50% of the diet consumed by control (R, n = 6). At 21 dg, after anestesia, the animals were perfused, the liver collected, weighed and processed by Hematoxylin-Eosin. The R dams tended to have a smaller liver mass in relation to body weight than C dams. It was observed a decrease in liver glycogen in R rats. Morphological analysis showed both the liver structure and the organization of hepatocytes similar between groups. The lobules did not present signals of inflammation, steatosis and fibrosis in both groups. Sinusoids vessels structure and distribution were similar. We hypothesize that maternal caloric restriction altered the liver size but not its structural organization. Study approved by CEUA/UNIARARAS (055/2014) CAMUNDONGOS C57BL6 TRANSGÊNICOS PARA A APOCIII HUMANA APRESENTAM REDUZIDA GLICOGENÓLISE E GLICÓLISE ADRENALINA-INDUZIDAS JAIRO AUGUSTO BERTI 1 ; MARIA M.D. PEDROSA 1 ; DIEGO G.L. REZENDE 1 ; MARCOS A. PRIMO 1 ; HELENA C.F. OLIVEIRA 2 ; VILMA A.F. GODOI 1 1.UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGA, PR, BRASIL; 2.UNIVERSIDADE ESTADUAL DE CAMPINAS, CAMPINAS, SP, BRASIL. Camundongos C57BL6 transgênico para apo-ciii humana apresentam alterações do metabolismo lipídico e glicêmico. Sendo assim, estudamos a glicogenólise e glicólise hepáticas através da técnica de perfusão de fígado in situ. Vinte camundongos machos (120d, 28g) alimentados foram utilizados. Os camundongos transgênicos (grupo CIII) apresentaram trigliceridemia>300mg/dl e os controles não transgênicos (grupo NTG) trigliceridemia<100mg/dl. Os fígados foram perfundidos com tampão por 30min (basal) e com tampão+adrenalina (1µM) por 30min (estimulado). Glicose, lactato e piruvato foram dosados (µmol/min.g fígado) e a área sob a curva (µmol/g fígado) calculada. CIB (3615/2013), CEUA (020/2013). Dados: média±dp, n=5/grupo; teste t, p<0,05. Na presença da adrenalina, o grupo CIII liberou mais piruvato (NTG=0,655±0,178, CIII=1,049±0,167; p<0,05), menos lactato (NTG=18,650±5,554, CIII=7,718±1,395; p<0,05) e glicose (NTG=68,235±7,432, CIII=25,955±3,959; p<0,05). Apresentou taxa reduzida de glicogenólise (NTG=42,778±2,231, CIII=16,251±1,275, p<0,05) e de glicólise (NTG=19,174±2,324, CIII=6,547±1,554, p<0,05), e reduzida razão NADH/NAD+citosólica (NTG=18,650/0,655=28,47, CIII=7,720/1,049=7,36). Camundongos C57BL6 apociii apresentaram: taxa glicolítica reduzida, efeito provável da inibição dasfosfofrutoquinase e piruvatodesidrogenase; glicogenólise e liberação hepática glicose reduzidas, indicando um possível efeito na atividade da glicogênio sintase pelos produtos da beta oxidação THE EXPRESSION OF CIRCADIAN CLOCK IN ANTERIOR PITUITARY GLAND IS ALTERED DURING THE ADULTHOOD HYPOTHYROIDISM PAULA BARGI-SOUZA; RODRIGO ANTONIO PELICIARI-GARCIA; RAFAEL MASO PRÉVIDE; MARIA TEREZA NUNES INSTITUTE OF BIOMEDICAL SCIENCES OF UNIVERSITY OF SÃO PAULO, SP, BRAZIL, SAO PAULO, SP, BRASIL. The anterior pituitary gland occupies a central position in the hypothalamus-pituitary-glands axes and the plasma concentrations of its hormones present fluctuations during the 24 h. The presence of an intra-pituitary circadian oscillator might be related to these oscillations; however, the consequences of the hypothyroidism are still unknown. The purpose of the present study was to investigate the expression of Bmal1, Per2, Dbp, Nr1d1, Rora and Dio2 during the adult hypothyroidism. For this, euthyroid (C) and thyroidectomized (Tx) male Wistar rats were euthanized during 24 h, every 6 h. The pituitaries were excised and the mrna expression was evaluated by RT-qPCR. One and Two-Way ANOVA, as well as, cosinor analysis were used to evaluate the time-of-day-dependent differential expression for each gene/group and their interactions. The expression of Bmal1, Per2, Dbp, Nr1d1, Rora and Dio2 presented a circadian pattern in anterior pituitary of C rats and the peak of Per2, Dpb, Rora and Dio2 expression occurred at ZT 12, while for Bmal1 was ZT 0/24. In the hypothyroid animals, the circadian pattern of Bmal1, Rora and Dio2 was lost and the acrophase of Per2, Dbp and Nr1d1 was advanced about 2.5 h, 3 h and 45 min, respectively. Tx also reduced Mesor values of Dbp and Nr1d1. Our studies reveal that the expression of core clock and clock-controlled genes in anterior pituitary gland are changed during the hypothyroidism and might contribute to the altered hormonal pattern of secretion observed in this pathological condition. Further studies are in progress to assess this issue GLICOGENÓLISE HEPÁTICA ESTIMULADA POR ADRENALINA PODE PARTICIPAR DO MELHOR PERFIL HIPOGLICÊMICO EM RATOS SOB RESTRIÇÃO ALIMENTAR DESDE O NASCIMENTO ANA MARIA PRAXEDES DE MORAES; CAMILA CRISTINA IANONE MATIUSSO; VILMA A.F. GODOI; MARIA M.D. PEDROSA UNIVERSIDADE ESTADUAL DE MARINGÁ, APUCARANA, PR, BRASIL. Uma sessão de exercício físico incremental antes de um episódio de hipoglicemia induzida por insulina (HII) eleva a glicemia basal, reduz a severidade do perfil hipoglicêmico e melhora a recuperação da glicemia em ratos sob restrição alimentar prolongada. Avaliou-se se a liberação hepática de glicose estimulada por adrenalina (gli-adr) poderia colaborar para esse quadro. Ratos Wistar machos do grupo controle (GC, ninhada de 6 filhotes, alimentação livre) e do grupo restrição (GR, ninhada de 12 filhotes, redução de 50% da alimentação), aos 60 dias de idade, após jejum noturno, foram submetidos à HII (1 U/kg) seguida de perfusão hepática in situ com ADR (1μM), 60 min e 300 min após a administração de insulina. Os dados são mostrados como média±dp, 13

14 n=5-8/grupo. Aos 60 min, a gli-adr foi maior no GR do que no GC (GC = 2,33±0,65, GR = 3,10±1,47 µmol.g-1, p<0,05, teste t). Aos 300 min, essa diferença foi ainda mais significativa (GC = 1,66±0,46, GR = 2,88±0,72 µmol.g-1, p<0,01, teste t). As taxas de glicogenólise também foram mais altas no GR do que no GC em ambos os tempos: GC = 2,66, GR = 3,80 µmol.g-1 aos 60 min; GC = 2,02, GR = 3,57 µmol.g-1 aos 300 min). Estes dados sustentam a hipótese de que a ADR liberada pelo exercício físico agudo, por meio da estimulação da glicogenólise hepática, pode ter contribuído para o perfil hipoglicêmico observado in vivo nos ratos sob restrição alimentar prolongada GLUTAMINA E GLUTAMINA DIPEPTÍDEO REDUZEM A GLICONEOGÊNESE EM RATOS COM DIABETES MELLITUS TIPO 1 DESCOMPENSADO SILVIA CARLA SANTANA AZEVEDO; MARIA M.D. PEDROSA; CARLOS VINICIUS DALTO DA ROSA; CAMILA BATAGLINI; DIEGO G.L. REZENDE; MARCOS A. PRIMO; ROBERTO BARBOSA BAZOTTE; VILMA A.F. GODOI UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGA, PR, BRASIL. A glutamina está reduzida no diabetes mellitus tipo 1 (DM1), mas os benefícios da sua suplementação não estão claros. Por isso, avaliou-se a gliconeogênese (GNG) em hepatócitos isolados de ratos Wistar machos DM1 (STZ, 60 mg/kg, iv) suplementados com glutamina (GLN) ou glutamina dipeptídeo (GDP). Os animais diabéticos formaram os grupos: D (salina oral); DGLN (248 mg/kg GLN oral); e DGDP (400 mg/kg GDP oral), tratados por 30 dias. Dados: média±dp, em μmol.106 cels.hr, n= 4/grupo, teste t, p<0,05. Em todos os grupos, a GNG na presença de precursores foi maior do que no estado basal, que foi: D=0,19±0,01; DGLN=0,14±0,03; DGDP=0,10±0,02. A GNG foi mais elevada a partir de lactato nos grupos D (0,89± 0,04) e DGDP (0,60± 0,02) e no DGLN a partir de glicerol (0,46±0,04). A GNG a partir de glicerol aumentou no grupo DGLN e diminuiu no DGDP em relação ao grupo D (D=0,36±0,03; DGLN=0,46±0,04; DGDP=0,26±0,02). A GNG a partir de lactato foi menor nos grupos DGLN (0,27±0,03) e DGDP (0,60±0,02) do que no grupo D (0,90±0,04), assim como a partir de glutamina (D=0,32±0,03; DGLN=0,22±0,04; DGDP=0,24±0,03). A GNG a partir de alanina não foi alterada (D=0,36±0,06; DGLN=0,36±0,07; DGDP=0,34±0,04). A gliconeogênese reduzida nos grupos DGLN e DGDP em relação ao grupo D poderia resultar em menor acúmulo de glicogênio pela via indireta e redução da glicogenose secundária, decorrentes do DM1 descompensado, o que aponta para um efeito benéfico da GLN e do GDP nesse quadro RESTRIÇÃO CALÓRICA MODERADA REDUZ A ADIPOSIDADE E A GLICEMIA DE JEJUM DE RATOS DE NINHADAS REDUZIDAS NAYRA DELATORRE BRANQUINHO; VILMA A.F. GODOI; MARIA M.D. PEDROSA; LETICIA DINIZ CREPALDI; GABRIEL HENRIQUE DE PAULA CRUZ; CAMILA BATAGLINI UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGA, PR, BRASIL; ; ;. A restrição calórica moderada é uma profilaxia contra os distúrbios metabólicos (Dmt) e cardiovasculares (Dcv) da obesidade. Foi registrado o padrão biométrico de ratos de ninhadas de 3 filhotes, um modelo de obesidade (NR), com alimento livre (NRL) ou sob restrição calórica de 30% (NRR), e de animais de ninhadas de 9 filhotes (controles, NC). Os ratos foram eutanasiados aos 21 e 90 dias de idade, após jejum noturno (14 hr). Dados mostrados como média±dp, n=4-11, analisados por teste t, p<0,05. Aos 21 dias, o NR teve valores maiores do que o NC quanto a peso corporal (47,1±2,3 vs. 39,5±3,1 g), gordura subcutânea (0,13±0,04 vs. 0,09±0,01 g/10g) e visceral (0,13±0,02 vs. 0,08±0,01 g/10g) e glicemia (123,4±22,6 vs. 102,3±13,5 mg/dl). Aos 90 dias, o peso corporal não diferiu entre o NRL e o NC, mas foi menor no NRR (NC=347,3±23,7; NRL=354,6±36,4; NRR=136,5±4,8 g). O mesmo foi observado para a gordura subcutânea (NC=1,22±0,22; NRL=1,32±0,28; NRR=0,90±0,20 g/100g) e a glicemia (NC=76,18±7,95; NRL=73,71±6,53; NRR=43,50±6,61 mg/dl). A gordura visceral foi maior no NRL do que no NC, e menor no NRR do que nos outros grupos (NC=2,78±0,45; NRL=3,58±0,97; NRR=1,19±0,16 g/100g.). A redução da ninhada (NR) e o alimento livre (NRL) aumentaram a gordura visceral, a mais prejudicial para Dmt e Dcv. A restrição calórica de 30% (NRR) reduziu acentuadamente o peso corporal e a adiposidade, mas a baixa glicemia de jejum é relevante pela importância desse parâmetro para a homeostase fisiológica ASSESSMENT OF THE AKT PHOSPHORYLATION STATUS AND THE GLUT4 EXPRESSION IN MUSCLE TISSUE OF RATS TREATED WITH NAF FERNANDO YAMAMOTO CHIBA; MARIANA LOPES BENITES; RENATO FELIPE PEREIRA; THAÍS SAORI TSOSURA; NATALIA FRANCISCO SCARAMELE; AMANDA GOMES PEREIRA; MURILO CARETA GUIMARÃES; RENAN AKIRA OLIVEIRA; DORIS HISSAKO SUMIDA UNIV ESTADUAL PAULISTA - UNESP, ARACATUBA, SP, BRASIL. Over the last years, there has been a significant reduction in the incidence of dental caries in several regions of the world. This has been attributed to the use of fluoridated products, such as toothpaste. However, excessive NaF intake causes glycolysis inhibition, decrease on insulin secretion and hyperglycemia, suggesting that NaF may cause insulin resistance. Therefore, became fundamental to examine the chronic effect of NaF on: 1) Akt phosphorylation status; 2) GLUT4 expression in skeletal muscle gastrocnemius (SMG). For this study, castrated Wistar male rats (1 month of age) were used. Thirty days after castration, the animals were be randomly divided in two groups: 1) control group (CN); 2) group NaF (FN) that was submitted to treatment with NaF administered in the drinking water and F contained in food pellets (F total inferred: 4.0 mg F / Kg bw / day in the form of NaF) during 42 days. After 6 weeks, the quantification of the Akt phosphorylation status, after insulin stimulation, in muscle, liver and white adipose tissue by western blotting method and the evaluation of GLUT4 expression in SMG by RT-PCR method were performed. Chronic treatment with NaF promoted: 1) a decrease in the Akt phosphorylation status in the muscle and in the white adipose tissue (p <0.05); 2) no change in the Akt phosphorylation staus in the liver; 3) reduction of GLUT4 expression in the SMG CALORIC RESTRICTION (CR) INCREASES INSULIN TOLERANCE BOTH IN HYSTERECTOMIZED OVARIAN SALPINGO RATS (OSH) AND IN SHAM RATS DANIELLA ROSALY LEITE; JÉSSICA TONETTI CUNHA; ÉRIKA CRISTINA VENTURA; THAÍS FURTADO CAMARGO; SABRINA MARTIN FELIPE; MATHEUS EDUARDO BORTOLANSA DA SILVA; MARIA ESMERIA COREZOLA AMARAL CENTRO UNIVERSITÁRIO HERMÍNIO OMETTO, ARARAS, SP, BRASIL. Absence of female hormones promotes metabolic changes as hyperphagia and the consequent weight gain associated to insulin resistance by peripheral tissues and diabetes type 2. The aim of this study was to investigate the effects of CR in such metabolic disorders. It was performed in female Wistar rats divided into groups: ShamC (diet ad libitum) ShamR (40% CR), OSHC (OSH + C) and OVHR (OSH + R). After eight weeks of surgery the rats were submitted to the CR for 28 days. It was analyzed glycemic and lipid profile, ip.gtt (the intraperitoneal glucose tolerance test), the ip.itt (intraperitoneal insulin tolerance test) liver and muscle glycogen. Blood glucose of ShamR and OSHR rats was less than ShamC and OSHC. There was a reduction in the perirenal adipose tissue of ShamR and OSHR rats compared to ShamC and OSHC. Data characterize the effects of CR. Cholesterol, triglyceride, ip.gtt and muscle glycogen were similar for all groups. OSHR had higher levels of liver glycogen compared to ShamC, ShamR and OSHC and suggests decreased mobilization of the substrate and consequent reduction of circulating glucose. The Kitt (constant for glucose decay calculated from ip.itt) was higher for ShamR and OSHR than to ShamC and OSHC and suggests suppression of gluconeogenesis and increased glucose uptake by insulinsensitive tissues. Data indicate that the rats submitted to RC, ShamR and OSHR, showed higher tolerance to insulin with changes of hepatic glycogen in OSHR group NEONATAL OVERFEEDING IMPAIRS HORMONAL CONTROL OF APPETITE AND BODY WEIGHT GAIN IN ADULTHOOD SILVIA ENES MARQUES; ALEXANDRE GIUSTI PAIVA FEDERAL UNIVERSITY OF ALFENAS, ALFENAS, MG, BRASIL. AIM:evaluate metabolic parameters, glucose-stimulated insulin secretion e anorexigenic effect of peripheral glucagon-like pepitide-1 (GLP-1) in neonatally overfed rats. METHODOLOGY:At postnatal day 1 (PND1), the litters (n=8) were adjusted to small litter (SL, n=3/litter) or normal litter (NL, n=12/litter). The body weight, plasma levels of glucose, triglycerides, cholesterol and its fractions, insulin and leptin, glucose (GTT) and insulin (ITT) tolerance tests and studies food intake were performed in males at PND 60. The procedures were approved by the CEUA UNIFAL (568/2014). RESULTS:The body weight was higher in the SL group since PND8 until adulthood compared to NL group (p<0.001). The males SL group presented increased plasma level of glucose (105±0.9 to 118±2.7mg/dL; p<0.001), triglycerides (135±11.7 to 169±10.6mg/dL, p<0.05), total cholesterol (70±3.7 to 85±4.1mg/dL, p<0.05), plasma leptin (0.68±0.1 to 1.92±0.2, p<0.01) compared with NL group. Males SL group exhibited decreased glucose tolerance (p<0.05) and reduced insulin sensitivity (p<0.001). GLP-1 (10 μg/kg) significantly reduced 30min (-57%, p>0.001), 60min (-66%, p>0.001), 90min (-60%, p>0.001), 120min (-45%, p>0.01) and 24h (-12%, p>0.05) food intake in males NL but did not alter the food intake SL rats. CONCLUSION: The neonatal overfeeding can disrupt the hormonal control of weight gain, of glucose homeostasis and ability of peripheral GLP-1 to inhibit food intake of adult rats ACTIVITIES OF NO- AND H2S-GENERATING HYPOTHALAMIC ENZYMES ARE BOTH INCREASED IN VITRO BY EXTRACELLULAR HYPEROSMOLALITY RICARDO COLETTI 1 ; FERNANDA MARIA VEANHOLI VECHIATO 1 ; JULIANA BEZERRA MEDEIROS DE LIMA 1 ; GISLAINE ALMEIDA-PEREIRA 1 ; FABIANA LUCIO-OLIVEIRA 2 ; LUCILA LEICO KAGOHARA ELIAS 1 ; JOSÉ ANTUNES-RODRIGUES 1 1.FMRP/USP, RIBEIRÃO PRETO, SP, BRASIL; 2.IFSULDEMINAS, MUZAMBINHO, MG, BRASIL. NO inhibits hypothalamic neurons depolarization which decreases vasopressin (AVP) and oxytocin (OT) release, while H2S induces depolarization that increases hormones release (HR); however, no experimental model of easy execution and that allows to assess NO and H2S generation and interaction in the medial basal hypothalamus (MBH) has been described. Thus we evaluated if the MBH incubation in vitro led to responses of HR and enzymatic activity (EA) according to the observed in vivo. To that, male Wistar rats ( g) MBH were collected and kept in isotonic (280 mosm/kg H2O) oxygenated Krebs-Ringer bicarbonate buffer (KRBG) for preincubation (37 C, 1L/min carbogenic mixture, 60 min). Then, medium was replaced by new hypertonic (340 mosm/kg H2O) or isotonic KRBG (incubation: 37 C, 1L/min carbogenic mixture, 30 min) from which samples were collected for AVP, OT and ANP radioimmunoassay. Afterwards, MBH were submitted to specific EA assays in which NO synthase (NOS) activity was measured by 14 C-citrulline generation and H2S-generating enzymes activity (CBS/3MST) was assessed by sulfides formation. MBH exposure to hypertonic KRBG increased AVP (t31 = 7.6, p <.0001), OT (t29 = 7.2, p <.0001) and ANP (t37 = 6.9, p <.0001) release, along with NOS (t12 = 2.2, p <.05) and CBS/3MST (t9 = 3.3, p <.01) activity, demonstrating that MBH incubation leads to response patterns congruent to that observed in vivo in relation to HR and NO and H2S formation in response to extracellular hypertonicity ANTI-DIABETIC EFFECTS OF ETHYL-ACETATE FRACTION FROM TRICHILIA CATIGUA IN STREPTOZOTOCIN-INDUCED TYPE 1 DIABETIC RATS RODRIGO MELLO GOMES; FLÁVIO ANDRADE FRANCISCO; CAMILA QUAGLIO NEVES; BRUNA COLOMBO CORDEIRO; VILMA A.F. GODOI; PAULO CESAR DE FREITAS MATHIAS; RENATA LONGHINI; JOÃO CARLOS PALAZZO DE MELLO; MARIA RAQUEL MARÇAL NATALI UEM, MARINGA, PR, BRASIL. Background: In Brazil Trichilia catigua A. Juss. is known as catuaba and have been popularly used as a tonic for the treatment of fatigue, stress, impotence and deficit memory. The present study evaluated the anti-diabetic activity of the ethyl-acetate fraction (EAF) of Trichilia catigua. Methods: Male Wistar rats were divided into four groups N: normal glycemic, D: diabetics, NC: normal glycemic + EAF (200 mg/kg) and DC: diabetic + EAF (200 mg/kg). Diabetes was induced by a single STZ (35 mg/kg) injection into the penile vein. Two days after STZ injection the EAF was orally administered 7 days per week, during 58 days, by gastric gavage. Results: Rats from D group showed reduced body weight, adipose tissue and muscular mass (p<0.05). This animals presented increased food and water intake and hyperglycemia with atrophy of pancreatic islets by β-cell death (p<0.05). However, EAF administration in DC group improves overall body mass loss and reduced food and water intake (p<0.05). In addition, EAF treatment in DC rats promoted an antidiabetic effect with 24% reduction in blood glucose (p<0.05). Furthermore, anti-insulin immunohistochemistry analysis shows significant increased β-cell number and islets size in DC group by β-cell proliferation labeled with PCNA immunohistochemistry. Conclusions: The present results suggest that EAF of T. catigua ameliorates glucose homeostasis and endocrine pancreas injury caused by STZ in diabetes type 1 rat model EFEITOS DE ESTEROIDES OVARIANOS, SORO FETAL BOVINO E TEMPO DE CULTURA SOBRE A VIABILIDADE DE OSTEOBLASTOS IN VITRO LUCAS FC BRANCAGLIÃO; VÍTOR M GONÇALVES; JOSÉ ES LEMOS; NÍCOLAS F ROCHA; RENATA AQUINO; ANDRÉ C AMARAL; LUIS HENRIQUE MONTREZOR CENTRO UNIVERSITÁRIO DE ARARAQUARA - UNIARA, ARARAQUARA, SP, BRASIL. O presente trabalho estudou os efeitos de diferentes concentrações (0, 10-8 M e 10-7 M) de progesterona (P4), testosterona (T) e estradiol (E2); do tempo de cultura (48 e 72 horas) e da ausência e presença de soro fetal bovino (SFB) sobre a viabilidade de osteoblastos in vitro. As células foram cultivadas em placas de 96 poços com meio DMEM suplementado com 100 U/mL de penicilina e 100 μg/ml de estreptomicina na densidade de 5 x 103 células/100 μl de meio, sem ou com 10% de SFB. As células foram mantidas em estufa a 37 ºC, 5% de CO2 e 95% de ar. A viabilidade celular foi analisada pelo ensaio colorimétrico do MTT. A viabilidade foi maior para as células cultivadas na presença de SFB em ambos os tempos e para todos os hormônios testados, comparado às culturas sem o SFB. E2, P4 e T estimularam a viabilidade das células cultivadas por 48 h na ausência de SFB; com 72 h de cultura, E2 e P4 estimularam a viabilidade celular, a T induziu efeito dual e todos os hormônios juntos estimularam a viabilidade. Na presença de 10% de SFB, com 48 h de cultura, a dose de 10-7 M de E2 estimulou a viabilidade celular e com todos os hormônios juntos, na dose de 10-7 M, houve redução da viabilidade. Com 72 h de cultura, a T e todos os hormônios juntos, na dose de 10-8 M, diminuíram a viabilidade das células. Os resultados sugerem que os esteroides ovarianos modulam a viabilidade dos osteoblastos, de maneira dosedependente, na ausência e na presença de SFB em 48 h e 72 h de cultura EVALUATION OF INSULIN SENSITIVITY AND AKT PHOSPHORYLATION STATUS IN MUSCLE TISSUE OF OVARIECTOMIZED RATS TREATED WITH SODIUM FLUORIDE AMANDA GOMES PEREIRA; RENATO FELIPE PEREIRA; FERNANDO YAMAMOTO CHIBA; THAÍS VERÔNICA SAORI TSOSURA; MARIA SARAA DE LIMA COUTINHO MATTER; DORIS HISSAKO SUMIDA UNIVERSIDADE ESTADUAL PAULISTA, ARAÇATUBA, SP, BRASIL. Sodium fluoride (NaF) has been studied as a possible therapeutic agent for osteoporosis in postmenopausal women. However, high doses of NaF can cause hyperglycemia, inhibition of glycolysis and decreased insulin secretion. The Akt protein participates actively in insulin signaling pathways by mediating the metabolic effects of this hormone in different tissues. The aim of this study was to evaluate the Akt serine phosphorylation status in the gastrocnemius muscle tissue (GM) and insulin resistance through the HOMA-IR index in ovariectomized rats chronically treated with NaF. Fourty Wistar rats were ovariectomized and randomly distributed in two groups: 1) control group (OVX-C); 2) group submitted to treatment with NaF (OVX-F) at a dose of 50 mg/l in drinking water for 42 days. Statistical analysis was performed by analysis of variance (ANOVA) followed by Tukey test. The significance level adopted was 5%. The treatment with NaF promoted: 1) decreased (p <0.05) Akt serine phosphorylation staus, after insulin stimulation, in GM; 2) increase (p <0.05) in the HOMA-IR index compared to the OVX-C group. It is concluded that chronic treatment with NaF (50 mg/l) induced insulin resistance and decreased insulin signaling in MG rats. Therefore, we suggest caution in the use of NaF for the treatment of osteoporosis in postmenopausal women PERIAPICAL LESION DECREASES GLUT4 TRANSLOCATION INDEX AND INCREASES IKK AND JNK PHOSPHORYLATION IN SKELETAL MUSCLE TISSUE IN RATS RENATO FELIPE PEREIRA; MARIANA LOPES BENITES; THAÍS SAORI TSOSURA; NATALIA FRANCISCO SCARAMELE; FERNANDO YAMAMOTO CHIBA; AMANDA GOMES PEREIRA; DORIS HISSAKO SUMIDA UNESP, ARAÇATUBA, SP, BRASIL. 14

15 Previous studies from our laboratory demonstrated that adult rats with periapical lesion (PL) showed changes insulin signaling and impaired insulin sensitivity. However, the mechanisms responsible for these changes are unclear. The kinases IKK (IkB kinase) and JNK-1 (c-jun N- terminal kinase 1) negatively regulate the insulin signaling. This study aimed to evaluate the GLUT4 translocation index to the plasma membrane, and the IKK and JNK phosphorylation in skeletal muscle of rats with PL. Wistar rats were divided into two groups: control (CN) and periapical lesion (PL). The PL was induced in the upper right molars employing carbon steel drill with sphere on the edge with 0.1 mm. The GLUT4 translocation index was calculated from the formula: GLUT4 translocation index (%) = plasma membrane GLUT4 x 100 / (plasma membrane GLUT4 + microsomal GLUT4). The IKK and JNK phosphorylation status were evaluated by Western blotting method. Statistical analysis was performed by Student's t test. The PL group showed a reduction of the GLUT4 translocation index to plasma membrane content and increased IKK and JNK phosphorylation in skeletal muscle. These results indicate that oral inflammation can cause inflammation in skeletal muscle which that impairs insulin signaling in this tissue SOLIDAGO CHILENSIS AND LASER ENHANCES THE REPAIR OF BURNS IN DIABETIC RATS VIVIANE THEODORO; JULIANA APARECIDA RAMIRO MOREIRA; ISRAEL COSTA VASCONCELOS; JOSÉ LUIS FACHI; FERNANDA OLIVEIRA GASPARI GASPI; RODRIGO AUGUSTO DALIA; MARCELO AUGUSTO MARRETO ESQUISATTO; FERNANDA APARECIDA SAMPAIO MENDONÇA; GLÁUCIA MARIA TECH SANTOS; MARIA ESMERIA COREZOLA AMARAL CENTRO UNIVERSITÁRIO HERMINIO OMETTO, ARARAS, SP, BRASIL; ; ; ; ; ; ; ;. Medicinal plants and low-intensity laser present a high potential in the treatment of wounds and burns. The objective of this study was to investigate the effect of the hydroalcoholic extract of the leaves of Solidago chilensis Meyen associated with the application of InGaP-670nm laser in repair of second-degree burns, were made on the back of diabetic Wistar rats. Thirty-two alloxan diabetic rats, characterized with glucose above 250 mg/dl, were anesthetized and the burn was performed with aluminum plate (2cm diameter/120 C/20s). The animals were divided into four groups: (C) no treatment; (S) treated with extract of S. chilensis; (G) treated with laser 670-nm InGaP; (LS) treated with laser associated with the S. chilensis extract. Samples were collected from lesions on the 7, 14 and 21 days for protein expression by Western blotting of TGF-β1, VEGF, bfgf, Collagen I and III. On the 14 day was observed a significant increase in VEGF expression in S and L. Both TGF-β1 expressions as bfgf were not significant during the experimental period in all groups. With respect to deposition of Collagen I was observed on the 14 day significant increase in L and LS groups and, on the 21 day, the S and L groups. The expression of Collagen III significantly decreased after 14 day in L and LS groups and, after 21 day, in the treated groups. S. chilensis and laser 670-nm InGaP promoted angiogenesis and collagen reorganization in repair burns in diabetic rats EFFECT OF CHRONIC STRESS ASSOCIATED TO HYPERCALORIC DIET CONSUMPTION ON THE ACTIVITY OF ANGIOTENSIN CONVERTING ENZYME AND RENIN, IN RATS FERNANDA KLEIN MARCONDES 1 ; RAFAELA COSTA 1 ; ANDREA SANCHES 1 ; FERNANDA RONCHI 2 ; ZAIRA PALOMINO JARA 2 ; DULCE ELENA CASARINI 2 1.FACULDADE DE ODONTOLOGIA DE PIRACICABA - UNIVERSIDADE ESTADUAL DE CAMPINAS, PIRACICABA, SP, BRASIL; 2.FEDERAL UNIVERSITY OF SAO PAULO, SAO PAULO, SP, BRASIL. Previously we ve observed that chronic mild and unpredictable stress (CMUS) induced dyslipidemia, endothelium dysfunction in the thoracic aorta, increased the activity of circulating and aortic renin angiotensin system in rats, and losartan cancelled vascular effect of CMUS. Stressed rats treated with hypercaloric diet (HD) also presented dyslipidemia and similar vascular effects. However non stressed rats treated with HD only presented dyslipidemia without vascular changes. In order to evaluate the relation among RAS activity, HD and CMUS effects, the aim of the present work was to study the effects of CMUS and HD on the activity of circulating and aortic angiotensin converting enzyme and plasma renin activity. Male rats were randomly assigned to four groups: Control, HD, CMUS, and HD-CMUS. CMS consisted of the application of different stressors for 3 weeks. The rats were killed 15 days after CMUS exposure. CMUS group presented higher activity of renin in plasma, and higher angiotensin converting enzyme activity in the thoracic aorta, in comparison with control, HD and HD-CMUS groups (p < 0.05), without difference among control, HD and HD-CMUS groups. CMUS, HD and HD-CMUS groups presented higher activity of seric angiotensin converting enzyme in comparison with control group. These results and the previous data indicate that vascular effects of CMUS observed in rats treated with HD, seem not to be related to changes in the activity of renin and angiotensin converting enzyme induced by CMUS EXERCÍCIO AGUDO INCREMENTAL MELHORA PERFIL HIPOGLICÊMICO EM RATOS SOB RESTRIÇÃO ALIMENTAR DESDE O NASCIMENTO POSSÍVEL EFEITO DA ADRENALINA SOBRE A GLICOGENÓLISE HEPÁTICA CAMILA IANONI MATIUSSO UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGA, PR, BRASIL. Uma sessão de exercício físico incremental melhora os episódios de hipoglicemia induzida por insulina (HII) em ratos sob restrição alimentar prolongada. Por isso, investigou-se a liberação hepática de glicose estimulada por adrenalina (gli-adr) em animais submetidos a exercício agudo. Ratos Wistar dos grupos controle (GC, ninhada de 6 filhotes, alimentação livre) e restrição (GR, ninhada de 12 filhotes, redução de 50% da alimentação), aos 60 dias de idade, após jejum noturno, foram submetidos a HII (1 U/kg) e perfusão hepática in situ com ADR (1μM), ambos com sessão prévia de exercício físico incremental em esteira. Os dados são mostrados como média±dp, n=5-8/grupo. A sessão de exercício antes da HII elevou a glicemia basal (GC=67,08±5,12, GR=92,21±8,09 mg.dl-1, p<0,01, teste t), reduziu a severidade do perfil hipoglicêmico e melhorou a recuperação da glicemia do GR (GC=39,12±9,6, GR=69,71±8,93 mg.dl-1 aos 300 min, p<0,01, teste t). A gli-adr foi maior no GR do que no GC após o exercício incremental (GC=1,46±0,21, GR=2,67 µmol.g-1, p<0,01, teste t). A gli-adr se mostrou preservada nos animais sob restrição alimentar desde o nascimento, mesmo após jejum noturno, o que justifica, ao menos parcialmente, a glicemia pós-exercício mais elevada, a hipoglicemia menos severa, e a melhor recuperação da glicemia durante o episódio de HII e após sessão aguda de exercício observada in vivo RESPOSTAS VENTILATÓRIAS E TÉRMICAS À HIPÓXIA E HIPERCAPNIA EM RATAS COM SÍNDROME DO OVÁRIO POLICÍSTICO LUIS HENRIQUE MONTREZOR 1 ; DÉBORA CARVALHO 2 ; JANETE APARECIDA ANSELMO-FRANCI 3 ; KÊNIA CARDOSO BÍCEGO 2 ; LUCIANE HELENA GARGAGLIONI BATALHAO 2 1.CENTRO UNIVERSITÁRIO DE ARARAQUARA - UNIARA, ARARAQUARA, SP, BRASIL; 2.FACULDADE DE CIÊNCIAS AGRÁRIAS E VETERINÁRIAS - UNESP, JABOTICABAL, SP, BRASIL; 3.FACULDADE DE ODONTOLOGIA DE RIBEIRÃO PRETO - USP, RIBEIRÃO PRETO, SP, BRASIL. Foram analisadas a ventilação pulmonar e a temperatura corporal durante hipercapnia (7% de CO2) e hipóxia (7% de O2) em ratas induzidas à síndrome do ovário policístico (SOP) com injeção de valerato de estradiol (VE) (2 mg/0,2 ml/rato) ou óleo (controle); 30, 45 ou 60 dias após a injeção de VE. Os ovários das fêmeas induzidas à SOP demonstraram alterações morfológicas típicas 30, 45 e 60 dias após a injeção de VE. As concentrações plasmáticas de estradiol (E2) e progesterona (P4) aumentaram nos grupos 30 (E2: 74,5 ± 23,5 pg/ml; P4: 18,3 ± 4,5 ng/ml), 45 (E2: 70,8 ± 17,5 pg/ml; P4: 21,3 ± 7,6 ng/ml) e 60 (E2: 71,4 ± 4,7 pg/ml; P4: 23,7 ± grupos 4,5 ng/ml) dias de VE enquanto que a testosterona (T) aumentou nos grupos 45 (58,4 ± 6,5 pg/ml) e 60 (79,5 ± grupos/ml) dias de VE em comparação com o grupo controle (E2: 46,9 ± 11,3 pg/ml; P4: 9,9 ± 3,4 ng/ml; T:. 22,6 ± 1,9 pg/ml). O tratamento com VE não afetou a ventilação em repouso nem a resposta ventilatória à hipóxia. No entanto, o aumento na ventilação induzida pela hipercapnia foi atenuada no grupo VE 30 dias (1978,9 ± 85,7 ml.kg -1.min -1 grupo controle; 1651,2 ± 369,3 grupo 45 dias e 1617,4 ± 131,4 grupo 60 dias vs 771,0 ± 172,4 grupo 30 dias). Houve redução da Tc induzida pela hipóxia em todos os grupos. Nossos resultados sugerem que as alterações iniciais promovidas pelo tratamento com VE atenua temporariamente o quimiorreflexo ao CO2, e estas respostas são compensadas em 45 e 60 dias após o tratamento. THEME 10 PHYSIOLOGY EDUCATION POPNEURO: POPULARIZING NEUROSCIENCE AND AWAKENING THE CURIOSITY OF SCHOLARS ABOUT THE NERVOUS SYSTEM GEORGIA ELISA FILIPIN; PAMELA BILLIG MELLO CARPES; BRUNA MEYER PERRONI UNIPAMPA, URUGUAIANA, RS, BRASIL. It is known that neuroscience has gained great prominence by his findings, especially when related to education. Considering this, our aim is to report a program of neuroscience popularization in public schools (POPNEURO). The activities were composed by weekly visits including a brief theoretical explanation and practical activity addressing neuroscience topics, held in four public schools of Uruguaiana/RS/Brazil. The proposed themes were: (i) What is science and neuroscience; (ii) The brain (neuroanatomy); (iii) How neurons talk to each other (synapse); and, (iv) Sensory perception. To evaluate the actions pre and post-intervention questionnaires were applied. 113 students from 9 to 13 years-old participated in the actions. At the beginning of the action (i) 63% of students said that they knew what a scientist makes; the percentage increased to 88% after the action. In action (ii) we find that only 4% of students knew what neuroanatomy is; at the end, 52% knew. In action (iii) the concept of synapse, were initially known by 4% of the students, but in the end by 84% of them. Prior to action (iv) only 10% of students know what is sensory perception, percentage reached 75% after the intervention. In general, the activities were well accepted, and students gave a grade of 9.6 (considering a scale of 0 to 10). Thus we see that the actions accomplished their goal of bringing neuroscience to the school, popularizing important concepts to the daily lives of students CAUSA E EFEITO NAS RESPOSTAS DE REGULAÇÃO DA PRESSÃO ARTERIAL: APLICAÇÃO DE UM JOGO DE PEÇAS JULIANA IRANI FRATUCCI DE GOBBI; SILVIA M NISHIDA DEPT PHYSIOLOGY, INSTITUTE OF BIOSCIENCE, UNESP, BOTUCATU, BOTUCATU, SP, BRASIL. A regulação da pressão arterial (PA) é um tema considerado complexo para os alunos de graduação. Geralmente, os alunos confundem a relação causa-e-efeito neste processo. Com a finalidade de incentivar a compreensão dos mecanismos de regulação da PA elaboramos um jogo onde os alunos escolhem, dentre várias possibilidades, o que representa a causa e a resposta dos mecanismos de controle da PA tendo como cenário da problematização, situações do cotidiano. A aplicação desta atividade ocorreu após aulas expositivas e estudos dirigidos sobre os conteúdos básicos. A turma foi dividida em 4 grupos, contando com 20 minutos para a resolução do problema. Cada grupo recebeu um caso e um conjunto de 20 figuras (extraídas do Google Image), palavraschave e setas indicando aumento e diminuição de uma determinada variável. As figuras foram escolhidas para preencherem um pôster onde deveria constar do lado esquerdo a causa e do lado direito a resposta para o caso em questão. Cada grupo compartilhou a resolução do seu caso para a turma. A atividade foi corrigida em conjunto para que toda a turma tivesse acesso a diferentes situações-problemas. Os alunos responderam um questionário para avaliação da atividade, onde 100% consideraram a dinâmica facilitadora para retenção do conhecimento. A experiência mostrou que as técnicas de ensino centradas nos alunos são mais efetivas para o processo de ensino-aprendizagem e motivadoras para ambos, professor e aluno STRUCTURE OF ASCENDING PATHWAYS OF THE NERVOUS SYSTEM (EVAS): TEACHING MODEL FOR LEARNING NEUROSCIENCES GIOVANA M CANCIAN 1 ; JOÃO LF BACCARELLI 1 ; JOSÉ DE ANCHIETA DE CASTRO HORTA JÚNIOR 2 ; JULIANA IRANI FRATUCCI DE GOBBI 3 ; MARCELO DM SASDELLI 4 ; PEDRO T HAMAMOTO FILHO 5 ; SILVIA M NISHIDA 3 ; TALITA HM SARTI 4 1.MARÍLIA MEDICAL SCHOOL, FAMEMA, MARÍLIA, SP, BRASIL; 2.DEPT ANATOMY, INSTITUTE OF BIOSCIENCE, UNESP, BOTUCATU, BOTUCATU, SP, BRASIL; 3.DEPT PHYSIOLOGY, INSTITUTE OF BIOSCIENCE, UNESP, BOTUCATU, BOTUCATU, SP, BRASIL; 4.MARÍLIA MEDICAL SCHOOL, FAMEMA, MARILIA, SP, BRASIL; 5.BOTUCATU MEDICAL SCHOOL, UNESP, BOTUCATU, SP, BRASIL. In the teaching and learning process, the teacher-student relationship must be focused on students as the main actors in the construction of knowledge. This paper is a case report in which a group of students from the Marilia Medical School planned and developed an educational model of the ascending pathways of the nervous system during the elective internship program at the Institute of Biociences UNESP. After the theoretical and practical study of neuroanatomy, neurophysiology and clinical cases of Neurology, aimed at a meaningful learning about structure and function of the nervous system and the deconstruction of a "neurophobic'' culture, it was proposed the production of teaching materials as an evaluation method. The group chose to produce material on the major ascending pathways. At the end of the internship program, they presented a didactic model of the neuroaxis, made of wooden vertical boards simulating cross-sections of the spinal cord, brainstem and forebrain.. Each plate was given stickers that represent internal structures of the central nervous system (nuclei and bundles/tracts), and was perforated for the passage of colored threads representing the ascending pathways, providing a three-dimensional visualization of the morphological and functional organization of the sensory system. The EVAS represented an innovative and facilitating experience of the teaching-learning process in neuroscience at the undergraduate level ACTIONS TO PROMOTE NEUROPHYSIOLOGY POPULARIZATION BRUNA MEYER PERRONI; GEORGIA ELISA FILIPIN; MAUREN ASSIS DE SOUZA; LIANE DA SILVA DE VARGAS; ALEXANDRE DOS SANTOS MARTINS; CAROLINE DALLA COLLETTA ALTERMANN; PAMELA BILLIG MELLO CARPES UNIVERSIDADE FEDERAL DO PAMPA, URUGUAIANA, RS, BRASIL. The importance of popularize and disseminate basic concepts of neurophysiology is known. The aim here is to report actions taken during the Brain Awareness Week (BAW) 2015 in Uruguaiana/RS/Brazil. The activities occurred between 18 and 21 of March in a public park and included four workshops: (i) Making art with the brain (drawing workshop); (ii) Neuroanatomy exibhition; (iii) Neuromyths; and, (iv) Optical illusions. To evaluate the activities a questionnaire was applied. 134 people participated in the activities, distributed in the workshops: (i) 38, (ii) 44, (iii) 25 and (iv) 27, being 65 children/adolescents, 26 young adults, 43 adults and 5 elderly. Half of the participants of the workshop (i) said that have already heard about neuroscience, however, 16 said to disregard the brain functions. Most participants of workshop (ii) (40) claimed to know the structure of the brain and consider important to know about it. During the workshop (iii) we realized that most participants (17) believed in neuromyths, however, after the discussions all participants were able to understand how the fact neurophysiological processes occur. Participants of workshop (iv) said that they have curiosity in understand optical illusions and almost all (26) affirm that understood the role of the brain in optical illusions. In the end, the participants attributed a final grade to each workshop: (i) 9,63; (ii) 9,79; (iii) 9,84; and, (iv) 9,81. The actions accomplished their goal and popularize concepts of neurophysiology in the community SESSÃO DE INTEGRAÇÃO COMO METODOLOGIA ATIVA E FERRAMENTA DE AVALIAÇÃO LUIS HENRIQUE MONTREZOR; KARIN FC PINOTTI; CYNTHIA M PIRATELLI CENTRO UNIVERSITÁRIO DE ARARAQUARA - UNIARA, ARARAQUARA, SP, BRASIL. A sessão de integração é realizada duas vezes por bimestre. É aplicada a todos os alunos do primeiro ao oitavo módulos. Os alunos são organizados em grupos (de 10 a 15 alunos) com um professor integrador para cada grupo. A atividade tem sessões de abertura e de encerramento. Na abertura os alunos recebem um caso clínico relacionado às áreas temáticas do módulo que cursam. Cada sessão tem um aluno coordenador e um relator. A sessão de abertura é composta pela leitura do caso, determinação dos termos desconhecidos, dos dados relevantes, da discussão do caso, das palavras-chave, da(s) hipótese(s) proposta(s) e da elaboração de objetivos para resolução dos objetivos propostos. A sessão de encerramento é iniciada com a leitura do caso, explicações dos termos desconhecidos, resolução dos objetivos propostos na sessão de abertura e discussão para adequações da(s) hipótese(s) proposta(s) na abertura. Os alunos são avaliados individualmente (em cinco ítens para cada sessão) pelo professor integrador em ambas as sessões. A duração de cada sessão é de 2 horas. A participação dos alunos é continuamente estimulada pelo aluno coordenador. Os resultados observados com tal metodologia são relevantes pois percebe-se que os estudos realizados para as sessões antecipam os temas que serão abordados 15

16 nas aulas teóricas e nas avaliações. Além disso, a integração entre os alunos durante as discussões dos casos prepara os mesmo para as discussões clínicas no hospital THE PERCEPTION OF STUDENTS ABOUT THE USE OF A PORTFOLIO TO LEARN THE PHYSIOLOGY OF THE ENDOCRINE SYSTEM ALINE SOARES MIRANDA; MICHELLE FRANZ-MONTAN; FERNANDA KLEIN MARCONDES FACULDADE DE ODONTOLOGIA DE PIRACICABA - UNIVERSIDADE ESTADUAL DE CAMPINAS, PIRACICABA, SP, BRASIL. The students of healthcare careers are expected to learn and to integrate the physiological concepts about endocrine system. The objective of this study was to evaluate the perception of student about the use of a portfolio to learn endocrine physiology. During physiology classes, in 2013 and 2014, 154 undergraduate students in Dentistry from Piracicaba Dental School have been requested to prepare in pairs a portfolio. This portfolio should have basic information about hormones action and secretion, relation with Dentistry, incorrect information found on the internet, and answers to specific questions presented by the professor. The portfolio was used as reference material to answer questions about clinical situations involving endocrine alterations in class, and during the assessment of learning. At the end of the semester, all the students answered the question: The use of the portfolio was helpful in learning endocrine physiology? Why?. For 142 students (92%), the portfolio was useful to enable them to understand the topics studied. These students evaluated that during the preparation of the portfolio they were encouraged to study alone and in groups, to know the importance of endocrine changes in dental practice. For 12 students (8 %) the portfolio was not useful because some students did not contribute in fact in the group. According to these data the use of the portfolio as an approach has been considered useful to learn endocrine physiology. In future studies, it will be evaluated if this practice in fact improve the learning about endocrine physiology PHYSIOLOGICAL UNDERSTANDING WEEK 2014: CONTRIBUTION OF COMMUNITY EXTENSION ACTIVITIES IN PHYSIOLOGY LEARNING CAROLINE DALLA COLLETTA ALTERMANN; BEN-HUR SOUTO DAS NEVES; RITHIELE GONÇALVES; PAMELA BILLIG MELLO CARPES UNIVERSIDADE FEDERAL DO PAMPA, URUGUAIANA, RS, BRASIL. The Physiological Understanding Week (PhUn Week) is an American outreach program that aims building connections between scientists and society through the discussion of physiology contents. So, we proposed to students enrolled in Human Physiology courses to organize community activities to promote the physiology dissemination during the PhUn Week 2014 in Uruguaiana/RS/Brazil. The activities were promoted between 03 and 07 of November. This work aimed to evaluate the contributions of this propose in the development of students physiology learning. 81 students divided into small groups carried out different activities with children, adults and seniors in schools and parks. After the activities, the students answered a questionnaire. 98% (n = 79) of the students considers that the proposal contributed to development of their learning, skills and competencies and 91% (n = 74) considers it important for their education and their understanding of physiology. In addition, the majority (86%; n = 70) said that it was possible to increase the understanding of people in the community about the physiology and its importance in their lives through the activities. Thus, the promoted outreach activities can be consider an important tool to help the build of knowledge, to increase the students interest and to disseminate important issues related to health and quality of life to community.

17 POSTER SESSIONS MONDAY AUGUST 3 17 Theme 01 Exercise Physiology Theme 02 Renal Physiology Theme 03 Neurophysiology Theme 04 Cardiovascular Physiology Theme 05 Gastrointestinal Physiology Theme 06 Celular Physiology Theme 07 Comparative Physiology Theme 08 Respiratory Physiology Theme 09 Endocrine & Metabolism Theme 10 Physiology Education THEME 01 - EXERCISE PHYSIOLOGY A PRIVAÇÃO DE SONO E O TREINAMENTO INTERVALADO DE ALTA INTENSIDADE AUMENTAM A CONCENTRAÇÃO DE GLUCAGON JORGE FERNANDO TAVARES DE SOUZA 1 ; MURILO DÁTTILO 1 ; MARCO TULIO DE MELLO 2 ; HANNA KAREN MOREIRA ANTUNES 1 1.UNIVERSIDADE FEDERAL DE SÃO PAULO, SANTOS, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL. Introdução: A privação do sono é uma condição deletéria para o metabolismo de carboidratos. Por outro lado, a prática do exercício físico é uma boa estratégia para a melhora do metabolismo em geral, e entre as diversas modalidades, o treinamento intervalado de alta intensidade (HIIT) destaca-se por proporcionar benefícios em um curto espaço de tempo. Objetivos: Analisar os efeitos da privação de sono e do HIIT sobre as concentrações de glucagon após 24 horas de privação de sono. Métodos: Onze voluntários jovens do sexo masculino foram submetidos a 4 condições: Sono Regular (SR), Privação de Sono (PS), HIIT + Sono Regular (HIIT + SR) e HIIT + Privação (HIIT + PS). HIIT consistiu de 6 sessões de treino (8-12 tiros, 60s a 100% da potência máxima com 75s de recuperação ativa) em bicicleta por 2 semanas. Após esse período, os voluntários dormiram ou foram privados de sono por 24 horas consecutivas. Pela manhã, amostras de sangue foram coletadas para avaliação da concentração de glucagon. Para a normalidade dos dados foi usado o teste de Shapiro-Wilk, e para comparação da concentração de glucagon foi usado ANOVA one-way com post hoc de Duncan e p 0,05 (NCT ). Resultados: A concentração de glucagon foi maior em HIIT + PS quando comparado às condições SR, PS e HIIT + SR, respectivamente. Conclusão: A privação de sono não foi capaz de alterar a concentração de glucagon. Contudo, a soma dos eventos estressores (HIIT e Privação de Sono) aumentou a concentração de glucagon AUMENTO DO TÔNUS VAGAL DESENCADEADO PELO TREINAMENTO RESISTIDO INFLUENCIA NA REDUÇÃO DO PERFIL INFLAMATÓRIO VENTRICULAR DE RATOS INFARTADOS DANIELE JARDIM FERIANI 1 ; MARCOS ELIAS ABSSAMRA 1 ; JANAINA DE OLIVEIRA BRITO 1 ; JULIANA CRISTINA OLIVEIRA 1 ; CATARINA DE ANDRADE BARBOZA 1 ; LUIZA MELANIE SILVA 1 ; LEANDRO YANASE ROCHA 1 ; NICOLLE MARTINS CARROZZI 1 ; CRISTIANO MOSTARDA 2 ; Mª CLAUDIA IRIGOYEN 3 ; ERICO CHAGAS CAPERUTO 1 ; BRUNO RODRIGUES 4 1.UNIVERSIDADE SÃO JUDAS TADEU, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DO MARANHÃO, SAO LUIS, MA, BRASIL; 3.UNIDADE DE HIPERTENSÃO, INCOR, FMUSP, SAO PAULO, SP, BRASIL; 4.FACULDADE DE EDUCAÇÃO FÍSICA, UNIVERSIDADE ESTADUAL DE CAMPINAS, CAMPINAS, SP, BRASIL. A atuação do treinamento resistido (TR) dinâmico no reflexo anti-inflamatório desencadeado pelo sistema nervoso parassimpático é pouco explorada. O objetivo deste estudo foi avaliar o TR na função cardiovascular, autonômica e perfil inflamatório de ratos Wistar machos submetidos ao IM. Os ratos foram divididos em grupos (n=10 cada): controle (C), controle + TR (CTR), IM sedentário (IS), IM + TR (ITR). Os grupos treinados foram submetidos a 3 meses de TR dinâmico (40-60% do teste de carga máxima (TCM) normalizado; 5 x semana). Fizemos avaliações ecocardiográficas, TCM (pré e pós intervenção), hemodinâmicas, de sensibilidade barorreflexa, função autonômica e concentração de citocinas inflamatórias no ventrículo esquerdo (pós). O TCM aumentou no grupo ITR (455±8 g) em relação ao IS (245±9 g). A área de IM e a fração de ejeção não alteraram, mas a espessura relativa de parede, a relação E/A e o índice de desempenho miocárdico melhoraram no ITR (0,48±0,04; 2,07±0,10; e 0,33±0,02) em relação ao IS (0,28±0,02; 2,69±0,05; e 0,54±0,04). Após o TR, o tônus simpático reduziu (50±4 bpm) e o tônus vagal aumentou (49±6 bpm) no ITR quando comparado ao IS (64±6 e 35±5 bpm). As concentrações de TNF-α, IL-10 e a razão IL-10/TNFα ventriculares sofreram ajustes positivos no ITR (20,5±3; 12,7±1 e 0,46±0,07 pg/ug prot) em relação ao IS (94,2±5; 38,5±5 e 0,38±0,02 pg/ug prot). Ocorreu correlação negativa entre o tônusvagal e a concentração de TNF-α nos grupos avaliados (r=0,78; p<0,0001). Sendo assim, sugere-se que o TR é benéfico no tratamento do processo inflamatório desencadeado pelo IM PHYSICAL EXERCISE OR TAURINE SUPPLEMENTATION REDUCES OXIDATIVE STRESS AND PREVENTS ENDOTHELIUM DYSFUNCTION IN HIGH-PALATABLE DIET-FED RATS CAMILA DE MORAES 1 ; LEANDRO KANSUKE OHAROMARI 2 ; NÁDIA FAGUNDES GARCIA 1 ; ALINE ROSA MAIA 3 ; ANA PAULA DAVEL 3 ; ELLEN CRISTINI DE FREITAS 1 1.SCHOOL OF PHYSICAL EDUCATION AND SPORT, UNIVERSITY OF SAO PAULO, RIBEIRÃO PRETO, SP, BRASIL; 2.DEPARTMENT OF INTERNAL MEDICINE, RIBEIRAO PRETO MEDICAL SCHOOL, UNIVERSITY OF SAO PAULO, RIBEIRAO PRETO, SP, BRASIL; 3.DEPARTMENT OF STRUCTURAL AND FUNCTIONAL BIOLOGY, UNIVERSITY OF CAMPINAS, CAMPINAS, SP, BRASIL. Excessive consumption of high-fat and refined sugar lead to metabolic disorders as obesity and its co morbidities. This study evaluated the effectiveness of physical exercise and Taurine supplementation to prevent vascular and metabolic disorders caused by high-palatable diet intake. Male Wistar rats ( g) were divided: sedentary; sedentary supplemented with 2% taurine solution; trained and trained supplemented with 2% taurine solution. Exercise training (treadmill at 60% maximal speed, 60min, 5 days/week) started after 4 weeks of high-palatable diet-fed and was carried out for further 7 weeks. Sedentary rats had increased levels of insulin, glucose and leptin. On the other hand, exercise effect only was seen to improve metabolic profile lowering levels of leptin, insulin and glucose. Impaired relaxation was seen in sedentary group, and it was improved not only by taurine supplementation but also by physical exercise. Increased expression of EC-SOD was seen in aortas from all treated groups. Physical exercise, in absence of taurine supplementation, was able to increase Cu-ZnSOD expression and reduce gp91phox. Superoxide formation in aorta was lower either in supplemented or in trained groups. In summary, not only physical exercise but also taurine supplementation were effective approaches to prevent endothelial dysfunction induced by high-palatable diet-fed GENE EXPRESSION PROFILE OF PROTEINS INVOLVED IN INFLAMMATION IN THE COMMISSURAL NUCLEUS OF THE SOLITARY TRACT (CNTS) AND ROSTRAL VENTROLATERAL MEDULLA (RVLM) IN SPONTANEOUSLY HYPERTENSIVE RATS (SHR) SUBMITTED TO SWIMMING EXERCISE (EX) ANDRÉA VANCETTO MAGLIONE 1 ; ANDRESSA VENDRAMINI 1 ; EDUARDO MAZUCO CAFARCHIO 1 ; BIANCA ALVES BIANCO 1 ; ITATIANA FERREIRA RODART 1 ; RUI MONTEIRO DE BARROS MACIEL 2 ; LEANDRO DE CARVALHO VALDO 1 ; GISELE GIANNOCCO 2 ; MONICA AKEMI SATO 1 1.FACULDADE DE MEDICINA DO ABC, SANTO ANDRE, SP, BRASIL; 2.FEDERAL UNIVERSITY OF SAO PAULO, SAO PAULO, SP, BRASIL. Aim: We investigated the gene expression profile of proteins involved in inflammation in the cnts and RVLM neurons in SHR submitted to Ex and sedentary (S) rats Methods: Adult male SHR (14-16 wks-old, N=6-7/group) were submitted to daily Ex bouts (1h/day, 5days/wk) with 2% b.w. load for 6 wks or were maintained S. One day after the last exercise bout, rats were anesthetized with 3% isoflurane in 100% O2, the brain stem was harvest and frozen in liquid nitrogen for RT-PCR procedures later on. The m-rna of cnts and RVLM neurons was extracted and the gene expression of cycloxygenase-2 (COX-2), interleukin 6 (IL-6), interleukin 10 (IL-10) and AT-1 receptor (AT-1r) was carried out by qpcr. Data was analyzed by Ct comparative method and are as mean±se, and submitted to unpaired Student t-test (p<0.05). Results: Gene expression (g.e.) of COX-2 increased in Ex-SHR in cnts (1.32±0.12 vs. 1.01±0.05S) and RVLM (1.27±0.08 vs. 1.01±0.06S). The IL-6 g.e. reduced in RVLM in Ex-SHR (0.14±0.1 vs. 1.04±0.28S), while IL-10 g.e. increased in Ex-SHR (1.24±0.10 vs.0.98±0.05s). The AT-1r g.e. decreased in Ex-SHR in cnts (0.69±0.05 vs. 1.03±0.08S) and RVLM (0.65±0.04 vs. 1.02±0.08S). Conclusion: The Ex induces adaptive responses in RVLM to reduce the oxidative stress dependent on AT-1r in order to reduce the inflammatory response that could be augmented in SHR LOW INTENSITY OF EXERCISE TRAINING IMPROVES CARDIOVASCULAR PARAMETERS IN PULMONARY HYPERTENSION RICARDO MEIRELLES BORBA 1 ; FERNANDA TIZZIANI SCHNEIDER 2 ; ANA AMÉLIA MACHADO DUARTE 2 ; JÉSSICA H.POLETTO BONETTO 3 ; RAFAEL DE OLIVEIRA FERNANDES 3 ; ALEXANDRE LUZ DE CASTRO 3 ; KATYA VIANNA RIGATTO 3 1.UNISC/UFCSPA, PORTO ALEGRE, RS, BRASIL; 2.UFCSPA, PORTO ALEGRE, RS, BRASIL; 3.UFRGS, PORTO ALEGRE, RS, BRASIL. Pulmonary hypertension (PH) is characterized by increase in pulmonary vascular resistance, leading to hypertrophy and failure of the right ventricle (RV), and autonomic nervous system (ANS) dysfunction. There is a reduction in heart rate variability (HRV) and an imbalance of the renin angiotensin system (RAS) increasing the vasoconstrictor axis. It remains unclear the ideal intensity of aerobic exercise training (ET) in PH. We evaluate the effect of low and moderate ET on the ANS and RAS enzyme expressions in the heart of PH-rats. PH was induced by a single injection of monocrotaline (MCT; 50mg/kg). Groups: control (CO), MCT-sedentary (MCT-S), MCT-low intensity (MCT-B), MCT-moderate intensity training (MCT-M). ET was performed on treadmill for 3 weeks. ANS was analyzed by HRV and enzyme expressions by western blot (WB) in the RV. Data were compared by two-way ANOVA follow by Tukey test (P<0.05). The RV weight and systolic pressure (RVSP) were higher (P<0.05) in MCT-S vs CO. HRV was higher (P<0.05) in the ET-groups than in sedentary groups (MCT-M and MCT-B vs MCT-S; MCT-B vs CO). Angiotensin converting enzyme (ACE) expression was decreased (P<0.05) in ET-groups, but ACE2 was increased (P<0.05) in all groups vs CO. Low intensity ET increases HRV, ACE2 expression, but attenuates RV hypertrophy, RVSP and ACE expression. Collectively, our results indicate that the low intensity ET is more effective than the moderated intensity to promote cardiovascular improvement in PH AEROBIC EXERCISE TRAINING PREVENTS FEMORAL ENDOTHELIAL DYSFUNCTION IN OBESE RATS AMANDA CHRISTINE SPONTON 1 ; HYGOR NUNES ARAUJO 2 ; CARMEM PERES VALGAS DA SILVA 2 ; CAMILA DE MORAES 3 ; EDSON ANTUNES 1 ; ANGELINA ZANESCO 2 ; MARIA ANDRÉIA DELBIN 1 1.UNIVERSIDADE ESTADUAL DE CAMPINAS, CAMPINAS, SP, BRASIL; 2.UNIVERSIDADE ESTADUAL PAULISTA JULIO DE MESQUITA FILHO, RIO CLARO, SP, BRASIL; 3.UNIVERSIDADE DE SÃO PAULO, RIBEIRÃO PRETO, SP, BRASIL. The aim of the study was to investigate the effects of aerobic exercise training (AET) on femoral artery responsiveness from rats fed with high-fat diet (HFD). Male Wistar rats were divided into: control sedentary (c-sd); control trained (c-tr); HFD sedentary(h-sd); HFD trained(h-tr). For 16 weeks, h-sd and h-tr groups were fed with HFD. After 8 weeks, c-tr and h-tr groups initiated the AET on treadmill (50-60% of maximum speed), 5 days/week, 60 min/session until the end of the protocol. Concentration-response curves to acetylcholine (ACh), sodium nitroprusside (SNP), phenylephrine (PHE) and the thromboxane A2 analogue (U46619) were obtained in femoral artery. Vascular protein expressions were determined. Biochemical parameters were analyzed systemically (CEUA:3203-1). The body weight, epididymal fat, triglyceride, insulin and leptin were increased in h-sd group whereas AET reduced these alterations in h-tr group. The maximal response to ACh was reduced in h-sd (62±4%) associated with decreased protein expressions of enos, Cu/Zn-SOD and leptin receptor. The AET normalized the relaxation response in h-tr group (73±1%) and also increased the expressions of enos, Cu/Zn-SOD and leptin receptor. No changes were observed to SNP, PHE and U46619, as well as, in adiponectin receptor, Mn-SOD and EC-SOD protein expressions. In conclusion AET for 8 weeks prevents femoral endothelial dysfunction in rats fed with HFD and that was possible associated with increased nitric oxide production and/or bioavailability THE INTAKE OF ICE DOES NOT IMPROVE PHYSICAL PERFORMANCE DURING A HALF- MARATHON IN THE HEAT MARCELO TEIXEIRA DE ANDRADE 1 ; WESLLEY MOURA SANTOS 1 ; MATHEUS MASCARENHAS SACCHETTO NUNES LEITE 1 ; ADRIANO ARAÚJO LOBO DO CARMO 1 ; DIOGO ANTONIO SOARES PACHECO 1 ; NEIMAR MARCOS DA SILVA 1 ; YGOR ANTONIO TINOCO MARTINS 1 ; NICOLAS HENRIQUE SANTOS BARBOSA 1 ; MOISÉS VIEIRA DE CARVALHO 2 ; SAMUEL PENNA WANNER 1 ; DANUSA DIAS SOARES 1 1.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSIDADE ESTADUAL DE MINAS GERAIS, IBIRITE, MG, BRASIL. Physical performance is reduced during prolonged exercise in the heat due to increased thermoregulatory, cardiovascular and metabolic strain. Many cooling strategies have been proposed to improve physical performance and reduce thermal strain, but the effects of ad libitum intake of ice are still unknown. Thus, this study analyzed the effects of the ingestion of different solutions (ice vs. water) in half-marathon performance. Ten men (27.8 ± 1.6 years; 60.2 ± 2.3 mlo2.kg-1.min-1) underwent four experimental trials, during which they should run 21.1 km as fast as possible in a hot environment (33 C, 60% RH): ad libitum ingestion of ice at -1 C (Ad-1); ad libitum ingestion of water at 37 C (Ad37); scheduled ingestion of ice at -1 C (Sch-1); scheduled ingestion of water at 37 C (Sch37). The two ad libitum trials were first conducted in a random order. During the Sch-1 and Sch37 trials, volunteers were induced to repeat the ad libitum intake pattern but ingested a different solution (e.g. in the Sch-1, the volunteers ingested ice at the same times and amounts that they ingested water in the Ad37). The 21.1 km running performance was not affected by the different solutions that were ingested (Ad-1: 107.0±4.2; Ad37: 111.3±5.2; Sch-1: 110.0±5.4; Sch37: 106.3±4.4 min). The ingestion of ice was not effective in changing the thermoregulatory, cardiovascular and metabolic strain during exercise. We conclude that ingestion of ice does not change performance in a half-marathon in the heat ACUTE EFFECT OF STATIC STRETCHING CLASS ON POST EXERCISE HYPOTENSION AND RATE PRESSURE PRODUCT KAROLYNE SILVA MAGALHÃES; RAONI DA CONCEIÇÃO DOS SANTOS; CESAR RAFAEL MARINS COSTA; WELINGTON VILLELA DE PAULA; WALLACE MARTINS VIANNA RIBEIRO; ANDERSON LUIZ BEZERRA DA SILVEIRA UFRRJ, RIO DE JANEIRO, RJ, BRASIL. The stretching it has been much sought, but their effects are not well described in the literature, so the purpose of this study was evaluate the acute effect of static stretching class on cardiovascular responses. The sample was composed by 15 untrained and healthy young men. After familiarization stretching class all subjects performed 45 minutes of 2 different protocols: STRETC (performing an active static stretching class) and CTRL (remaining at rest) with 48h of interval between them, in a cross-over design. The systolic (SBP), diastolic (DBP) and mean blood pressure (MBP), heart rate (HR) and rate-pressure product (RPP) were measured before and immediately after the protocols. The statistical procedure performed was two-way ANOVA and the significance adopted was p We observed that before stretching class there was no difference between protocols for all described variables (p>0,05). However, after stretching class was found significant increase to SBP, MBP, HR and RPP between protocols. On the other hand, there was not post exercise hypotension induced by STRETC during 25 min. at rest. These results showed that stretching class can increase acutely blood pressure and cardiovascular stress, but this exercise could not be able to induce post exercise hypotensive effect. Thus, stretching exercise should be given with caution.

18 THE EFFECTS OF ONE NIGHT OF PARADOXAL SLEEP DEPRIVATION (PSD) ON PHYSICAL PERFORMANCE AND THERMOREGULATORY RESPONSES OF RATS SUBJECTED TO AN INCREMENTAL EXERCISE UNTIL FATIGUE FELIPE LIOE TEH SHANG; SAMUEL PENNA WANNER; LUCIANO SALES PRADO UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL. This study evaluated the effect of one night of PSD on exercise time and thermoregulatory responses of rats subjected to incremental exercises until fatigue in hot and temperate environments. The study was approved by the local ethics committee (CEUA 273/2014). Nine male Wistar rats were subjected to two incremental-speed, fatiguing treadmill running in a temperate environment (24oC): normal sleep condition - control (C24) or after one night of PSD (PSD24); and two incremental exercises in a hot environment (31oC): C31 and PSD31. The order of the trials was randomized. The rats running time to fatigue were measured. Sleep-deprived rats presented higher Trec prior to exercise, but similar rate of heat storage and Trec at the end of exercise when compared to their respective control trials in both environments. At 24 C, sleep-deprived rats presented a lower exercise performance when compared to the control trial, but no significant differences in performance were observed at 31 C. PSD reduces exercise performance in the temperate environment, but heat may represent to the animals a higher physiological strain than sleep deprivation, so that no differences in performance were observed between the two exercise trials in the heat. Higher Trec prior to exercise seem not to influence final Trec, but may play a role in performance reduction in the temperate environment DRINK TEMPERATURE AFFECTS DRINKING BEHAVIOR OF ATHLETES DURING A SELF- PACED RUNNING EXERCISE IN THE HEAT MARCELO TEIXEIRA DE ANDRADE 1 ; WESLLEY MOURA SANTOS 1 ; MATHEUS MASCARENHAS SACCHETTO NUNES LEITE 1 ; ADRIANO ARAÚJO LOBO DO CARMO 1 ; DIOGO ANTONIO SOARES PACHECO 1 ; NEIMAR MARCOS DA SILVA 1 ; GUSTAVO GUIMARÃES AGUIAR DE OLIVEIRA 1 ; RÚBIO SABINO BRUZZI 1 ; THIAGO TEIXEIRA MENDES 2 ; SAMUEL PENNA WANNER 1 ; DANUSA DIAS SOARES 1 1.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSIDADE FEDERAL DO MARANHÃO, SÃO LUÍS, MA, BRASIL. Long distance races are popular and challenging sporting events, and many of them are performed in hot environments. The aim of this study was to evaluate the pattern of voluntary intake of drinks with different temperatures in runners during half-marathon in the heat. In a balanced and randomized order, ten male volunteers (27.8 ± 1.6 years; 73.5 ± 2.6 kg; 178 ± 2 cm; ± 2.3 mlo2.kg-1.min-1) were subjected to two experimental trials, during wich they ingested ice (-1ºC) or water (37ºC) ad libitum, while running 21.1 km as fast as possible in a hot environment (33 C, 60%RH): The drink temperature influenced the pattern of voluntary intake in runners, as evidenced by the difference in the total amount that was ingested (ice: ± 58,5g vs. water: ± 135.7g) and by the average intake per aliquot (ice: 66.5 ± 6.9 g vs. water: ± 10.3 g; p<0.05). However, the average number of aliquots that was ingested was not different between the two experimental conditions (ice: 5.3 ± 0,8 vs. water: 5.1 ± 0.8) (p>0.05). We conclude that, although the number of times that athletes seek for the ingestion of ice and water is similar, the ice has a lower palatability than water during a self-paced running in the heat THE EFFECTS OF FOUR NIGHTS OF PARADOXAL SLEEP DEPRIVATION (PSD) ON PHYSICAL PERFORMANCE AND THERMOREGULATORY RESPONSES OF RATS SUBJECTED TO AN INCREMENAL EXERCISE UNTIL FATIGUE FELIPE LIOE TEH SHANG; GABRIELA CANGUSSU CASTRO; YGOR ANTONIO TINOCO MARTINS; SAMUEL PENNA WANNER; LUCIANO SALES PRADO UFMG, BELO HORIZONTE, MG, BRASIL. This study evaluated the effect of four nights of PSD on physical performance and thermoregulatory responses of rats subjected to incremental-speed exercises until fatigue in hot and temperate environments. The study was approved by the local ethics committee (CEUA 273/2014). Twelve male Wistar rats were subdivided in two groups of 6 rats each. Both groups were subjected to an incrementalspeed, fatiguing exercise on a treadmill after 4 nights of PSD or under normal sleep conditions (control). One group of rats exercised in both conditions (control C24 and sleep deprived PSD24) in a temperate (24oC) environment while the other group exercised in both control (C31) and sleep-deprived conditions (PSD31) in a hot (31oC) environment. The rats rectal temperature (Trec) and running time to fatigue were measured. Sleep-deprived deprived rats presented higher Trec prior to exercise, similar rate of heat storage and lower performance when compared to their respective control trials in both environments. Trec at the end of exercise was different between control and sleep-deprived rats only in temperate environment. PSD reduces exercise performance and changes the thermoregulatory responses of running rats in both temperate and hot environments. The altered thermoregulatory response in sleep-deprived rats can explain their lower running time only in the heat THE EFFECTS OF ELECTRICAL STIMULATION ON THERMOREGULATORY RESPONSES AND PERFORMANCE OF RATS SUBJECTED TO A PHYSICAL EXERCISE WILLIAM COUTINHO DAMASCENO; FILIPE SALVADOR ZINATELLI COELHO; ANA CANCADO KUNSTETTER; SAMUEL PENNA WANNER UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL. Electrical stimulation is commonly used to encourage rats to run on a treadmill. This study evaluated the effects of electrical stimulation on thermoregulatory responses and performance of rats subjected to a physical exercise. Seven adult male Wistar rats ( g) were used in the experiments. Under anesthesia, a temperature sensor was implanted in the abdominal cavity of the rats. After four days of recovery, the rats were familiarized with the treadmill running (5-day protocol, electrical stimulation on) and then subjected to two experimental trials at 24 C: incremental-speed running (starting at 10 m/min, with increases of 1 m/min every 3 min) until volitional fatigue, with or without electrical stimulation. The abdominal temperature (Tabd) and tail skin temperature (Ttail) were measured during the exercise. In both experimental trials, the treadmill running increased Tabd and Ttail 8 and 16 min after the exercise had initiated, respectively. During the initial moments of exercise, there were no differences in Tabd and Ttail between the experimental trials. However, the animals subjected to electrical stimulation ran more and achieved higher Tabd and Ttail at volitional fatigue compared with animals non-subjected to electrical stimulation. The electrical stimulation does not affect thermoregulatory responses during the initial moments of exercise, but increases physical performance and consequently the Tabd and Ttail at volitional fatigue COMPOSIÇÃO CORPORAL E RENDIMENTO FÍSICO: ALTERAÇÔES PRODUZIDAS PELO EXERCÍCIO FÍSICO EM RATOS JOVENS TRATADOS COM A DIETA RICA EM CARBOIDRATO SIMPLES MARIANA ARAÚJO VIEIRA DO CARMO; ANGÉLICA BARBOSA GONÇALVES PINTO; ÉLIDA MERCÊS RAIMUNDO; LENICE KEPPES BECKER UNIVERSIDADE FEDERAL DE OURO PRETO, OURO PRETO, MG, BRASIL. Objetivo: Analisar o efeito do treinamento físico sobre as alterações produzidas pela dieta rica em carboidrato simples (DCS) em ratos jovens. Metodologia: Ratos Wistar com 21 dias foram submetidos a 4 (T4) e 8 semanas (T8) de treinamento em natação com sobrecarga, 1 hora/dia, 5 vezes/semana. Os ratos dos grupos sedentário não realizaram exercício (SE4 e SE8). Foi feito um teste de carga máxima (TCM) pré e pós-treinamento para avaliar o rendimento físico. O Índice de Lee (IL) e o Índice de adiposidade corporal (IAC) foram calculados no dia do sacrifício. Os dados foram expressos como média ± desvio padrão. Foram utilizados o teste ANOVA, seguido de pós teste de Bonferroni e teste Kruskal Wallis, seguido de pós teste de Dunns. Resultados: Não houve diferença no delta de tempo de exercício no TCM entre os grupos SE4 (-3,7 ± 2 minutos) e T4 (- 3,4 ± 2,3 minutos), no entanto, houve diferença significativa entre os grupos SE4 (-3,7 ± 2 minutos) e T8 (0,06 ± 4 minutos). O IL não indicou diferença (SE4 1,7 ± 0,05 vs T4 1,7 ± 0,1). Após 8 semanas, T8 (1,7 ± 0,04) mostrou menor IL em comparação com SE8 (1,8 ± 0,11). O IAC não diferiu entre os grupos (SE4 2,3 ± 0,26 % vs T4 2,6 ± 0,51 %; SE8 2,2 ± 0,44 % vs T8 2,1± 0,81%). Conclusão: Os resultados sugerem que a associação entre DCS e exercício físico, promove alterações no rendimento físico que são dependentes do tempo de treinamento CORRELATIONS AMONG THE CHANGES IN MUSCLE, CORE AND SKIN TEMPERATURES DURING EXPOSURE TO A COLD ENVIRONMENT GUSTAVO GUIMARÃES AGUIAR DE OLIVEIRA 1 ; CHRISTIAN EMMANUEL TORRES CABIDO 1 ; DIEGO CAMPOS DE MOURA 1 ; NATHÁLYA GARDÊNIA DE HOLANDA MARINHO NOGUEIRA 1 ; THIAGO FONSECA DOS SANTOS 1 ; MATHEUS MILANEZ DOS REIS 1 ; EMERSON SILAMI GARCIA 2 ; SAMUEL PENNA WANNER 1 ; MAURO HELENO CHAGAS 1 1.UFMG-MG, BELO HORIZONTE, MG, BRASIL; 2.UFMA, SÃO LUIZ, MA, BRASIL. The purpose of this study was to verify the association level among the muscle temperature (MT) measured in vastus lateralis (VL), rectal temperature (RT) and skin temperature (ST) during exposure to a cold environment (10 C;60 min). Six male volunteers (age=27.0±3.3 years; body mass=76.5±11.5 kg: average ± DP) were analyzed. An ultrasonography device was used to measure the thickness of the VL, which was divided by two and then summed to subcutaneous tissue; this calculation allowed us to determine the depth at which the sensor was inserted. Next, the VL area was sterilized and a cannula (18G) was inserted in the thigh of the subjects. The temperature sensor was inserted in the muscle through the guide cannula and, after 30 min of rest, the volunteers were directed to an environmental chamber, where they remained seated, wearing only shorts, socks and sneakers. The Pearson s correlation coefficient was applied to evaluate the association level among MT, RT and TS during the 60 min of exposure. The cold exposure decreased body core, muscle and skin temperatures by 37.02±0.25 to 36.76±0.40 C, 33.70±1.37 to 29.94±1.80 C and 28.02±0.93 to 22.95±1.39 C, respectively. The results also indicated significant, high and positive correlations between MT and RT (r=0.89;p<0.05) and MT and TS (r=0.91;p<0.05). In conclusion, although the magnitude of the temperature responses of these compartments is different, these responses to changing external temperatures are interconnected TRANSGENIC RATS WITH INCREASED CIRCULATING ANGIOTENSIN-(1-7) HAVE LESS MUSCLE DAMAGE AFTER EXERCISE TO EXHAUSTION NÁDIA LÚCIA TOTOU 1 ; MARIANA FLÁVIA OLIVEIRA 2 ; LENICE KEPPES BECKER 1 1.UNIVERSIDADE FEDERAL DE OURO PRETO, OURO PRETO, MG, BRASIL; 2.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL. Angiotensin-(1-7) [Ang-(1-7)] Ang-(1-7)/Mas axis plays a critical role in muscle damage in different experimental models. Purpose: The aim was investigate the influences of higher circulating levels of Ang-(1-7) on muscle damage markers after physical exercise. Methods: Sprague Dawley (SD) and in TGR(A1-7)3292 (TG) rats which present approximately two fold increase in plasma Ang-(1-7) levels compared to SD were submitted to exercise until exhaustion with a weight equivalent to 4% of body weight tied to the tail. Blood samples were taken to analyze the creatine kinase (CK), lactate desidrogenase (LDH), and α-actin levels on skeletal muscle. Student t test was used to compare possible differences between groups. Results: Total exercise time was not different between groups TG (5.1 ±0.3 hours) and SD (4.8 ± 0.3 hours). The results obtained to muscle damage markers show that LDH was lower in TG rats 158±19 U/L in comparison with SD rats 211±10 U/L. Equally, α-actin was lower to TG 224 ±29 U/L in comparison with SD rats 360 ±55 U/L, CK values was not different between groups 468 ± 16 U/L to TG vs 462 ±13 to SD group. Conclusion: This study shows that Ang-(1-7) can be effective in prevent the muscle damage induced by exercise until exhaustion SYMPATHO-VAGAL CHANGES INDUCED BY EXERCISE TRAINING IN AGEING SPONTANEOUSLY HYPERTENSIVE RATS LAÍS OLIVEIRA DELLACQUA 1 ; CHARLIE HINDMARCH 2 ; JULIAN FRANCIS RICHMOND PATON 2 ; DAVID MURPHY 2 ; LISETE COMPAGNO MICHELINI 1 ; VAGNER ROBERTO ANTUNES 1 1.USP, OSASCO, SP, BRASIL; 2.UNIVERSITY OF BRISTOL, BRISTOL, REINO UNIDO. The practice of physical exercise is related to an improvement of hypertensive damages, because it leads to an increase in parasympathetic tone as well as a decrease in sympathetic tone, leading to a reduction in blood pressure. The aims of this study were to evaluate the sympathetic-vagal balance and hemodynamic components in exercise-trained (T) ageing SHR. SHR and WKY rats, 12- months-old were trained 5 days/week for 8 weeks. SHR (T8) had a significant reduction in the SBP (155±11 mmhg, n=7) when compared to the sedentary (S8) group (198±10 mmhg; n=7, p<0.05). In addition, the spectral analysis of the heart rate variability have shown a higher HF index of SHR (T8) (22.4±3.4; n=5) compared to the S8 (6.3± 2.4; n=5). The LF/HF ratio was lower in SHR trained for 8 weeks (0.21 n=5) when compared with control animals sedentary (0.43 n=5), an indicative of higher parasympathetic tone on these animals. Collectively, our data show that physical exercise for 8 weeks in ageing SHR elicits benefits in the cardiovascular system by reducing systolic blood pressure and increasing in the parasympathetic tone to the heart TRANSGENIC RATS WITH INCREASED CIRCULATING ANGIOTENSIN-(1-7) HAVE BETTER METABOLIC PROFILE AFTER EXERCISE TO EXHAUSTION ANGÉLICA BARBOSA GONÇALVES PINTO 1 ; MARIANA FLÁVIA OLIVEIRA 2 ; LENICE KEPPES BECKER 1 1.UNIVERSIDADE FEDERAL DE OURO PRETO, OURO PRETO, MG, BRASIL; 2.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL. The Angiotensin-(1-7) [Ang-(1-7)] plays a critical role in metabolic pathways involved in glucose control. The aim of this study was investigates the influences of higher circulating levels of Ang- (1-7) on glucose responses and glycogen content after extraneous physical exercise. Methods: Sprague Dawley (SD) and in TGR(A1-7)3292 (TG) rats which present approximately two fold increase in plasma Ang-(1-7) levels compared to SD were submitted to exercise until exhaustion with a weight equivalent to 4% of body weight tied to the tail. Blood samples were taken to analyze the glycemic response to exercise; liver and muscle tissue glycogen content were evaluated. Student t test was used to compare possible differences between groups. Results: Despite the total exercise time was not different between groups TG (5.1 ±0.3 hours) and SD (4.8 ± 0.3 hours) the exercise induced a lower decrease in glucose serum levels in TG (-13±10 mg/dl) rats in comparison with SD rats (-41±7 mg/dl). TG rats were able to save glycogen content; the content of TG rats was higher after exercise in muscle (0.12 ±0.02 mg/g) and liver (2.24±0.8 mg/g) when compared with SD rats (0.06± mg/g to muscle, 0.65±0.13 to liver). Conclusion: This study shows that Ang-(1-7) has important role in metabolic control during exhaustive exercise O EXERCÍCIO FÍSICO EM MEIO AQUÁTICO SENSIBILIZA MAIS O BARORREFLEXO EM COMPARAÇÃO COM MEIO TERRESTRE EM RATOS ESPONTANEAMENTE HIPERTENSOS ÉLIDA MERCÊS RAIMUNDO; MARIANA ARAÚJO VIEIRA DO CARMO; LENICE KEPPES BECKER UFOP, OURO PRETO, MG, BRASIL. O treinamento físico em meio aquático produz respostas fisiológicas distintas das observadas em meio terrestre. Objetivo: comparar o efeito do exercício físico em meio aquático e terrestre sobre a sensibilidade barorreflexa em ratos espontaneamente hipertensos (SHR). Metodologia: Ratos SHR foram submetidos a 8 semanas de treinamento físico em piscina (PIS) e esteira (EST). Ao final das 8 semanas, os animais foram anestesiados por via intraperitoneal, para canulação da artéria femoral para registro da pressão arterial média (PAM) e veia femoral para administração de fenilefrina (FE, 8µg/ml). Os dados foram expressos em média ± erro padrão da média. Resultados: O teste de carga máxima mostrou que houve aumento do rendimento físico para os grupos PIS (6±3 minutos) e EST (8±2 minutos) em comparação com o grupo sedentário (-1±1 minuto). A resposta da PAM à fenilefrina não foi diferente entre os grupos EST (54 ± 6mmHg) e PIS (56±9mmHg), porém a resposta bradicardica a FE foi significativamente maior para o grupo PIS (- 109 ± 26 bpm) em comparação ao grupo EST (-54±14 bpm) Conclusão: Estes dados mostram que o meio onde o exercício físico é realizado pode modular as respostas do barorreflexo em ratos SHR INFLUENCE OF NEONATAL MATERNAL SEPARATION ON PHYSICAL PERFORMANCE OF RATS DURING PROGRESSIVE EXERCISE JULIANA BOHNEN GUIMARÃES 1 ; FREDERICO SANDER MANSUR MACHADO 2 ; DANIELA COSTA ROCHA FÓSCOLO 2 ; PAULO MARCELO A. LIMA 2 ; CÂNDIDO CELSO COIMBRA 2 1.UNIVERSIDADE DO ESTADO DE MINAS GERAIS, IBIRITÉ, MG, BRASIL; 2.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL. 18

19 In childhood, maternal cares are the main sources of stimuli for proper development, growth and adaptive responses to stressors. Deprivation of maternal presence can break this protection and promotes alterations in metabolism due to change in neuronal program. Thereafter, the main was to verify if maternal separation (MS) during the first days of life interferes with thermoregulation and consequently the physical performance in rats during exercise. On the 14th day of postnatal life, the litters were maternal separated (MS) which consisted in removing the mother from the cage for 180 min, while leaving the pups undisturbed. The control animals were left undisturbed throughout this period. After two months from weaning, it was implanted an intraperitoneal sensor in order to measure the core body temperature (Tc). After recovery, rats underwent a progressive exercise (PE) in a treadmill (the velocity was increased by 1 m/min in each 3 minutes - initial speed 10 m/min), until fatigue. MS reduced exercise time, maximal velocity and workload (P < 0.05). PE induced an increase in Tc in both groups. Tc at exercise onset and fatigue was not different between groups (fatigue C: 38.4 ± 0.4 C vs. MS: 38.5 ± 0.5 C; P = 0.5). From these data, it was calculated the heat storage rate which showed lower values in C compared to MS (P < 0.05). As conclusion, MS reduces performance during PE until fatigue. In addition, heat storage rate could have contributed to this result. These results point to the importance of the early maternal care for future development of individuals AEROBIC EXERCISE RESTORES CENTRAL ACTION OF INSULIN IN SPONTANEOUSLY HYPERTENSIVE RATS (SHR) IZABELA MARTINA RAMOS RIBEIRO; LAÍS OLIVEIRA DELLACQUA; ANA CAROLINA PANVELOSKI-COSTA; NEWTON SABINO CANTERAS; VAGNER ROBERTO ANTUNES USP, SÃO PAULO, SP, BRASIL. Insulin is an important hormone in the control of glucose production at the hepatic territory, but little is known about its action on modulation of autonomic nervous system (ANS) in the control of glicemia in hypertension, usually associated to insulin resistance and metabolic dysfunction. Previous studies from our laboratory demonstrate significant impairment in the central action of insulin in modulating hepatic glucose production in SHR (IMR Ribeiro et al, 2015). The present study shows that aerobic exercise training for 4 weeks (T4) promotes a significant fall in hepatic vein blood glucose levels at 5 minutes after insulin injection (100nU/1ul) in the lateral ventricle (LV) in 18 weeks old SHR animals (-18% at 5 min, -24% at 10 min, with a maximum decreasing at 30 min (23%, n=6) compared at the same time course to the sedentary (S) SHR group (0% at 5 min, 4% at 10 min, and 10% at 30 min after central injection of insulin; n=6). However, there was no significant difference in peripheral insulin sensitivity in the T4 (increases 13%) compared to the sedentary group, demonstrated by rate constant for insulin tolerance test (kitt). Our results suggest that physical exercise promotes an improvement in central action of insulin in SHR, which is suppose to be due to amelioration of central sensitivity of this hormone, since its peripheral action was not affected by aerobic exercise. THEME 02 - RENAL PHYSIOLOGY INSULIN, ALBUMIN AND GLUCOSE DOWNREGULATE GLUT2 PROTEIN EXPRESSION IN LLC-PK1 PORCINE PROXIMAL TUBULE CELL LINE JENIFFER PEREIRA DA CRUZ GONCALVES UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, MACAE, RJ, BRASIL. Until now the role of albumin, glucose and insulin in the regulation of GLUT2 protein expression in kidney proximal tubule is not well known. The aim of this work was evaluate cell viability and GLUT2 expression in LLC-PK1 under insulin, albumin and glucose treatments. The cells were treated 24h with starved medium (CTRL) or starved medium supplemented with insulin (ng/ml - 5, 50 and 500), albumin (mg/ml , 0.1, 1, 10 and 20) and glucose (mm - 5, 10, 20 and 40). Cell viability was determined using MTT assay and GLUT2 protein expression was determined using Western Blot. Statistical analysis were performed using One-Way ANOVA followed by Dunnet postest (mean ±SEM, p < 0.05). It was observed that relative GLUT2 expression (times of control) was lower in cells treated with insulin at 5, 50, 500 (0.84±0.037, 0.81±0.045 and 0.72±0.046), glucose at 10, 20 and 40 (0.46±0.040, 0.57±0.080 and 0.65±0.12) and albumin at 0.1, 1 and 10 (0.74±0.059, 0.74±0.052 and 0.71±0.078), compared to CTRL (1.00 ± 0, n = 4, p < 0.05). The cell viability was higher in cells treated with insulin at 50 and 500 (1.4±0.10 and 1.5±0.14) and albumin at 10 and 20 (1.45±0.058 and 1.88±0.089) compared to CTRL (1.0±0, n = 12, p < 0.05). No significant changes in cell viability was observed after high glucose concentration treatment. High concentrations of albumin, insulin and glucose are able to downregulate GLUT2 expression and only insulin and albumin are able to increase cell viability PAPEL DO RECEPTOR EXTRACELULAR PARA CÁLCIO (CASR) NA REGULAÇÃO DA H+- ATPASE PRESENTE EM CÉLULAS DO NÉFRON DISTAL ANNA GABRIELLE COUTINHO; RICARDO FERNANDEZ PEREZ UNIVERSIDADE FEDERAL DO PARANÁ (UFPR), CURITIBA, PR, BRASIL. Vários fatores tem sido identificados como reguladores diretos e indiretos da atividade da H + ATPase vacuolar presente nos segmentos distais do néfron, dentre eles: íons como Na +, K + e Ca ++ ; e hormônios como ADH e PTH, Angiotensina II e Aldosterona. Num estudo recente do nosso laboratório utilizando porções de membrana plasmática parcialmente purificadas da região cortical e medular externa do rim de camundongo foi observado que o estímulo do receptor sensível a cálcio, o CaSR, pelos agonistas Gd 3+ ou neomicina incrementou de forma significativa a atividade bioquímica da H + ATPase do tipo vacuolar e da H + /K + -ATPase do tipo gástrica. O objetivo do presente estudo é investigar a interação do CaSR com a atividade da H + ATPase num modelo de células renais do ducto coletor de mamíferos, as células MDCK. A atividade bioquímica da H + ATPase foi determinada em um homogenado de células MDCK por um método colorimétrico modificado do descrito por Fiske-Subarow, onde a diferença entre o Pi liberado na ausência e na presença de concanamicina 10-8 M corresponde à atividade da mesma. Os resultados parciais apontam que a estimulação do CaSR com neomicina 300μM ou Gd μM incrementa a atividade da H + ATPase vacuolar: 31,1±9,9nmol Pi.mg -1.min -1 (n= 9) atividade basal, 53±15,2nmol Pi.mg - 1.min -1 (n= 8) com neomicina e 44,7±9nmol Pi.mg -1.min -1 (n= 9) com Gd PRESSÃO ARTERIAL E PARÂMETROS DA FUNÇÃO RENAL DE RATOS WISTAR DE NINHADA REDUZIDA ORIUNDOS DE FÊMEAS EXERCITADAS DURANTE A GESTAÇÃO E LACTAÇÃO LETICIA DINIZ CREPALDI UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGA, PR, BRASIL. A alta prevalência da obesidade infantil e adulta envolvem fatores relacionados ao estilo de vida, tais como: grande consumo energético e baixos níveis de atividade física. Em ratos, a redução do tamanho da ninhada é um modelo de indução de sobrepeso/obesidade. Considerando esse contexto, o presente trabalho teve como objetivo analisar parâmetros da função renal e pressão arterial média (PA) da prole submetida à redução de ninhada, oriundas de ratas exercitadas em esteira (30% VO2max) durante a gestação e lactação. Os animais foram divididos em dois grupos: G-CON (controle mãe exercitada n=8) e G-RN (redução de ninhada mãe exercitada n= 9). Aos 90 dias de vida, foi realizado registro da pressão arterial média, coleta de sangue, medida da ingestão hídrica, coleta da urina, análise da proteinúria e da taxa de filtração glomerular (TFG). Não houve alteração significativa na PA (G-CON= 101,31 ± 12,53 e G-RN= 94,35 ± 7,76 mmhg), no fluxo urinário (G-CON =0,02 ±0,008 e G-RN = 0,033 ± 0,02mL/min), na proteinúria (G-CON = 3,93 ±2,7 e G-RN = 6,07 ±2,06mg/13h) e na TFG (G-CON = 0,9 ±0,4 e 0,7 ±0,2mL/min/100gpc) da prole submetida à redução de ninhada. Portanto, o exercício físico de baixa intensidade (30% VO2max), durante a gestação e lactação, não alterou pressão arterial, taxa de filtração glomerular e proteinúria da prole submetida à redução de ninhada AEROBIC EXERCISE PREVENTS ACUTE KIDNEY INJURY ESDRAS GUEDES FONSECA 1 ; MARKUS BERGER 2 ; LEDA MARIA DE CASTRO COIMBRA CAMPOS 1 ; ROBERTA DA SILVA FILHA 1 ; TATIANE ALMEIDA 1 ; MARIA APARECIDA RIBEIRO VIEIRA 1 1.UFMG, BELO HORIZONTE, MG, BRASIL; 2.UFRGS, PORTO ALEGRE, RS, BRASIL. There are few studies reporting the possible kidney adaptations induced by aerobic exercise (AE), especially in acute kidney injury (AKI). In this study, we investigated the effect of aerobic exercise of moderate intensity, on the prevention of AKI induced by gentamicin (GM). The whole procedure was approved by the Ethics Committee on Animal Experiments (CETEA No 182/08). Before the start of training, 28 Wistar rats ( g) underwent a progressive maximal exercise test (TEMP). Soon after, the animals were divided into two groups: not trained (NT) and trained (T). The training protocol consisted of group T treadmill running at moderate intensity at 65% of maximum speed achieved in TEMP (60 min / day, 5 days / week for 8 weeks). After the training period, the rats were placed individually in metabolic cages. The two groups rats were divided into subgroups and treated subcutaneously with saline(sal,0.9% NaCl); NT-SAL and T-SAL and gentamicin(gm,gentatec, 80 mg / kg / day, 0.1 ml/100g); NT-GM and T-GM for 5 days. Urine and blood samples were collected before and after the treatment, for analysis of renal function. The AE prevented the decrease in glomerular filtration rate (GFR) induced by GM. Separately, GM and AE produced increased kidney expression of RNA for ACE. The previous PT prevents the increase of the expression of ACE and reduced expression of AT1 receptors. These data indicate that AE prevents the kidney damage induced by GM, possibly because of a minor activation of the vasoconstrictor axis of angiotensin system CHRONIC FISH OIL SUPPLEMENTATION REVERTED PARTIALLY RENAL ALTERATIONS ON MICE FED WITH A HIGH-FAT DIET ISABELA COELHO DE CASTRO 1 ; DANIELLE CRISTINA TONELLO PEQUITO 2 ; GINA BORGHETTI 3 ; ADRIANA AYA YAMAGUCHI 4 ; GLEISSON A. P. DE BRITO 5 ; LUIZ CLAUDIO FERNANDES 6 ; TEREZILA MACHADO COIMBRA 7 ; RICARDO FERNANDEZ PEREZ 6 1.UFLA, LAVRAS, MG, BRASIL; 2.UFMS, TRÊS LAGOAS, MS, BRASIL; 3.UFRR, BOA VISTA, RR, BRASIL; 4.INSTITUTO DE PESQUISA PELÉ PEQUENO PRÍNCIPE, CURITIBA, PR, BRASIL; 5.UNIVERSIDADE FEDERAL DA INTEGRAÇÃO LATINO-AMERICANA (UNILA), FOZ DO IGUAÇU, PR, BRASIL; 6.UFPR, CURITIBA, PR, BRASIL; 7.FMUSP-RB, RIBEIRÃO PRETO, SP, BRASIL. The present work was evaluated the effect of chronic fish oil (FO) supplementation on renal morphology and function of mice fed with a high-fat diet (HFD). Six weeks male mice were divided in two groups and fed during eight weeks with regular chow (R), or HFD. After this, they were supplemented orally for 30 days with FO, 1g/kg body weight. Renal function parameters, urinary excretion of TXB2, PGE2 and albumin, immunolocalization of vimentin, desmin were measured. The weight gain of HFD group was higher, almost 2 times, than R group. HFD group presented glucose intolerance, an effect that was not modified by FO. Creatinine clearance was lower in HFD (0.81±0.09 ml/min/kg body wt) when compared to R (1.28±0.22) (p<0.05), independent of FO supplementation. HFD induced albuminuria: 35.65±7.32ng/mg in HFD vs 11.06±2.16 in R (p=0.01). FO supplementation reduced in 40% albumin excretion in HFD. Urinary TXB2 excretion was reduced in 58% by high-fat diet and FO was not able to reverse this modification. Urinary PGE2 excretion was similar among groups. The HFD was increased in almost 100% the concentration of TNF-α in renal tissue; an effect reverted by FO supplementation. The intrarenal expression of vimentin was significantly higher in tubulointerstitial cells of HFD group, an effect reverted by FO. Desmin expression in glomerulus edge increased in obese animals, but was not modified by FO supplementation. In conclusion, FO reverted partially renal alterations induced by a HFD SLEEP RESTRICTION DURING PREGNANCY ALTERS RENAL FUNCTION IN FEMALE RAT OFFSPRING ROGÉRIO ARGERI 1 ; GUIOMAR NASCIMENTO GOMES 2 ; SERGIO TUFIK 3 1.UNIFESP, SÃO PAULO, SP, BRASIL; 2.PROFESSORA DO DEPARTAMENTO DE FISIOLOGIA - UNIFESP/EPM, SÃO PAULO, SP, BRASIL; 3.PROFESSOR DO DEPARTAMENTO DE PSICOBIOLOGIA - - UNIFESP/EPM, SÃO PAULO, SP, BRASIL. The reduction of sleeping time leads to hormonal and metabolic alterations. Moreover, changes in maternal environment may modify the development of the offspring leading to development of diseases at adulthood. Objectives: Evaluate renal function of female offspring from rats sleep restricted during the last week of pregnancy. Methods: Pregnant Wistar rats were divided in 2 groups: control and sleep restricted. The sleep restriction was carried out between 14th and 20th day of pregnancy, 20 h/day. Female offspring: Control (C, N=17) and Sleep Restriction (SR, N=26) were studied at 8 months old. Parameters studied: indirect BP (BPi tail cuff), urea ([P]u) and creatinine ([P]cr) plasma concentration, creatinine clearance (CLcr), Na+ excretion (CENa+), urine flow (V), protein excretion (PT-ur), number of glomeruli per field (Nglo) and renal mass (Rmass). Data showed as mean±sem, P 0.05, t-student. Results: SR group presented increased values of BPi [C: 124.9±0.7; SR: 130.8±0.5 mmhg]; smaller CLcr [C: 4.6±0.3; SR: 3.4±0.2 ml/min/kg]; increased PT-ur [C: 5.0±0.4; SR: 6.5±0.5 mg/24h], and reduced Rmass [C: 0.7±0.02; SR: 0.6±0.01 g/100g bw]. There were no significant differences in the others parameters. Conclusion: Sleep restriction during pregnancy changed renal development leading to alterations in renal function and in blood pressure in offspring at later life EVALUATION OF VASOPRESSIN IN SITU ADMINISTRATION IN URINARY BLADDER AND GENE AND PROTEIN EXPRESSION PROFILE OF VASOPRESSIN RECEPTOR SUBTYPES IN FEMALE WISTAR RATS EDUARDO MAZUCO CAFARCHIO 1 ; ITATIANA FERREIRA RODART 1 ; BIANCA ALVES BIANCO 1 ; GISELE GIANNOCCO 2 ; RUI MONTEIRO DE BARROS MACIEL 2 ; DANIEL PAULINO VENÂNCIO 1 ; MONICA AKEMI SATO 1 1.FMABC, SANTO ANDRE, SP, BRASIL; 2.UNIFESP, SÃO PAULO, SP, BRASIL. Aim: This study investigated the effects of in situ vasopressin administration in different doses in urinary bladder and gene and protein expression of vasopressin receptors. Materials and Methods: Female Wistar rats (~250 g, N= 6/group) were anesthetized with 2% isoflurane in 100% O2, the urinary bladder was cannulated to measure the intravesical pressure (IP). After the baseline recording, it was administrated approximately 0,1 ml the vasopressin at different concentrations (1 ng/ml, 0,5 ng/ml and 0,25 ng/ml) or saline on urinary bladder. Data are as mean±se and were submitted to unpaired Student t-test (p<0.05). Separated groups of animals were used to qpcr and western-blot, respectively, in order to evaluate the vasopressin receptor subtypes (V1a, V1b and V2). Results: The vasopressin increased the IP (107±3, 59±4 e 56±4% in the doses of 1ng/mL, 0,5ng/mL e 0,25ng/mL, respectively) compared to saline (2±1%). We observed the gene and protein expression of all subtypes of vasopressin receptors in the urinary bladder Conclusion: In situ administration of vasopressin increased the IP, demonstrating the existence of vasopressin receptors in urinary bladder as either the gene and protein expression showed all subtypes of vasopressin receptors in this organ THE ROLE OF THE RHOA PATHWAY IN NHE3 ACTIVITY DURING PERFUSION OF RAT PROXIMAL TUBULES WITH SIMVASTATIN OR SILDENAFIL PRISCILLA MARYS COSTA DOS SANTOS; THAISSA DANTAS PESSOA; GERHARD MALNIC UNIVERSIDADE DE SÃO PAULO, SAO PAULO, SP, BRASIL. Sil is a drug that increases cgmp and inhibits RhoA, and this intracellular pathway has pronounced inhibitory effects on NHE3. Simva modulates RhoA, and could alter the rate of endocytosis and exocytosis of NHE3 increasing its expression in the apical membrane, and its activity. The aim of this work was to determine the effects of Simva or Sil on NHE3 and to study intracellular signaling pathways involved. By means of stationary microperfusion, PT of Wistar rats were perfused with a 24mM HCO3- solution (CTRL) with or without 100µM Simva or 10µM Sil to determine bicarbonate reabsorption (JHCO3-). Perfusion of Sil decreased JHCO3- by 20% and perfusion of Simva increased JHCO3- by 19,31% compared to CTRL. To test if these effects were NHE3-dependent, PTs were perfused with CTRL, Sil or Simva plus 2µM S3226, a specific NHE3 inhibitor. The reminiscent S3226- insensitive JHCO3- was not different among groups, showing that NHE3 was modulated by Sil or Simva. To determine if RhoA was involved in these effects, the PTs were perfused with the Rho GTPase inhibitor Y (1µM). Perfusion of Y alone decreased JHCO3- by 20% compared to CTRL. Moreover, the perfusion with Y and Sim reversed the stimulatory effect of Rho on JHCO3- and increased the inhibitory effect of Sil by 26.16%. These data show that the increase of JHCO3- by Simva was mediated by the RhoA pathway; and the decrease of JHCO3- by Sil was also RhoA dependent. THEME 03 - NEUROPHYSIOLOGY 19

20 P2X PURINERGIC RECEPTOR BLOCKADE REDUCES NACL INTAKE IN FLUID DEPLETED RATS TREATED WITH ALPHA2-ADRENERGIC AGONIST IN THE LATERAL PARABRACHIAL NUCLEUS CAMILLA DIAS GUILLEN; GLAUCIA M. F. DE ANDRADE-FRANZÉ; PATRICIA MARIA DE PAULA; LAURIVAL ANTONIO DE LUCA JR; JOSÉ VANDERLEI MENANI; CARINA AP. FABRÍCIO DE ANDRADE DEPT. PHYSIOLOGY AND PATHOLOGY, SCHOOL OF DENTISTRY, FOAR - UNESP, ARARAQUARA, SP, BRASIL. Alpha2-adrenergic and P2X purinergic receptor activation in the lateral parabrachial nucleus (LPBN) increases fluid depletion induced 0.3 M NaCl intake. In the present study, we investigated 0.3 M NaCl intake induced by fluid depletion in rats treated with bilateral injections of moxonidine (alpha2-adrenoceptor/imidazoline receptor agonist) combined with P2X purinergic receptor blockade in the LPBN. Male Holtzman rats (n = 5) with cannulas implanted bilaterally in the LPBN were submitted to subcutaneous treatment with furosemide (FURO, 10 mg/kg) and captopril (CAP, 5 mg/kg) to induce sodium appetite. Bilateral LPBN injections of moxonidine (0.5 nmol/0.2 μl) increased 0.3 M NaCl intake (27.9 ± 8.6, vs. vehicle: 2.7 ± 1.0 ml/120 min). The P2X purinergic antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS, 2 nmol/0.2 μl) alone into the LPBN did not change 0.3 M NaCl intake (2.9±1.1 ml/120 min). However, the pre-treatment with PPADS into the LPBN abolished the effects of moxonidine on 0.3 M NaCl intake (8.5 ± 4.1 ml/120 min). These results suggest a possible interaction between alpha2-adrenergic and P2X purinergic receptors in the LPBN to control sodium intake. Fnancial support: FAPESP, CNPq, PROPe-UNESP EFFECTS OF A GREEN TEA ON MEMORY AND OXIDATIVE BALANCE AFTER DIFFERENT STROKE MODELS IN RATS CAROLINE DALLA COLLETTA ALTERMANN; ALEXANDRE GARCIA DOS SANTOS; ALEXANDRE DOS SANTOS MARTINS; HELEN LIDIANE SCHIMIDT; MAUREN ASSIS DE SOUZA; PAMELA BILLIG MELLO CARPES UNIVERSIDADE FEDERAL DO PAMPA, URUGUAIANA, RS, BRASIL. Stroke could be ischemic or hemorrhagic; both cause functional alterations and cognitive impairment. Stroke also promotes reactive oxygen species (ROS) increase in brain. Here we investigate the effect of green tea (GT) on memory and oxidative damage induced by transient ischemia-reperfusion (IR) and intracerebral hemorrhage (IH). 80 Wistar male rats divided into 8 groups according the stroke type induced were used (n=10/group): Sham IR, Sham IR+GT, IR, IR+GT, Sham IH, Sham IH+GT, IH, IH+GT. Supplementation with GT was initiated 10 days before stroke surgery and continuous for 7 days after (GT dose 400 mg/kg). After all, the rats were subjected to object recognition task (OR) to assess short (STM) and long term memory (LTM) and hippocampus were used to measure parameters related to oxidative stress (ROS, lipid peroxidation and total antioxidant capacity). OR results were analyses using one-sample t-test (theoretical mean 50%) and biochemical parameters using one-way ANOVA and Dunn s post hoc. IR and IH rats showed STM and LTM deficits; GT intervention prevents STM and LTM deficits in both stroke models (P= STM IR; P= STM IH; P<0.05 LTM IR; P= LTM IH). IR and IH induces increase in ROS concentration (P = 0.04 IR, P =0.006 IH) and lipid peroxidation (P= IH) in the hippocampus; GT supplementation avoids it. The oxidative imbalance and damage observed in IR and IH rats could be related to the memory deficits. GT supplementation presented a neuroprotective role POSSIBLE INVOLVEMENT OF THE INHIBITORY MECHANISMS OF THE LATERAL PARABRACHIAL NUCLEUS IN THE CONTROL OF SODIUM INTAKE IN RENOVASCULAR HYPERTENSIVE RATS RICHARD BOARATO DAVID 1 ; CAMILA FERREIRA RONCARI 1 ; MARIANA RUIZ LAUAR 1 ; REGINA CELIA VENDRAMINI 2 ; DEBORA SIMÕES ALMEIDA COLOMBARI 1 ; CARINA A.F. ANDRADE 1 ; JOSÉ VANDERLEI MENANI 1 ; LAURIVAL ANTONIO DE LUCA JR 1 1.DEPTO. FISIOLOGIA E PATOLOGIA, PIPGCF UFSCAR-UNESP, FACULDADE DE ODONTOLOGIA, UNESP, ARARAQUARA, SP, BRASIL; 2.DEPTO. ANÁLISES CLÍNICAS, FACULDADE DE CIÊNCIAS FARMACÊUTICAS, UNESP, ARARAQUARA, SP, BRASIL. Rats with 2 kidney, 1 clip (2K1C) renal hypertension have sustained increase in plasma renin activity and arterial blood pressure from the 3rd up the 6th week after surgery. Daily 0.3 M NaCl intake increases in these rats in the 3rd and 4th week post-surgery (~ 30 ml/day) and returns to control levels in the 5th-6th week (~ 18 ml/day). Inhibitory mechanisms of angiotensin II-induced sodium intake are present in the lateral parabrachial nucleus (LPBN) and may act to reduce sodium intake chronically in 2K1C hypertensive rats. Thus, we tested 0.3 M NaCl intake in 2K1C hypertensive rats with blockade of LPBN mechanisms by local activation of α2-adrenoceptors. Male adult Holtzman rats (n = 4-7/group), with stenosis of the left renal artery (2K1C hypertensive) and others with sham surgery, had daily access to both water and 0.3 M NaCl. Moxonidine (0.5 nmol/0.2 μl), an α2-adrenoceptor agonist, injected into the LPBN on the 7th week produced an immediate and substantial 0.3 M NaCl intake in normohydrated 2K1C rats (20.5 ± 6.2, vs. vehicle: 0.6 ± 0.4 ml/2 h), but had no effect on sham rats (1.8 ± 1.1, vs. vehicle: 0.1 ± 0.1 ml/2 h). The results suggest recruitment of LPBN inhibitory mechanisms to limit daily sodium intake after the 4th week of stenosis in 2K1C hypertensive rats BEHAVIORAL AND HISTOCHEMICAL CHANGES INDUCED BY NA+/GLUCOSE COTRANSPORTER 1 (SGLT1) INHIBITION IN MODEL OF TEMPORAL LOBE EPILEPSY IGOR SANTANA DE MELO 1 ; YNGRID MICKAELLI OLIVEIRA DOS SANTOS 1 ; AMANDA LARISSA DIAS PACHECO 1 ; MAÍSA DE ARAÚJO COSTA 1 ; NÍVEA KARLA DE GUSMÃO TAVEIROS SILVA 1 ; MARCELA SAMPAIO DE ARAÚJO 1 ; ROBINSON SABINO-SILVA 2 ; OLAGIDE WAGNER DE CASTRO 1 1.UNIVERSIDADE FEDERAL DE ALAGOAS, MACEIO, AL, BRASIL; 2.UNIVERSIDADE FEDERAL DE UBERLÂNDIA, UBERLÂNDIA, AL, BRASIL. We evaluated the functional role of Na+/glucose cotransporter 1 (SGLT1) in the behavior of limbic seizures and neurodegeneration process. Experimental procedures were approved by the Ethical Committee for Animal Research of UFAL (10/2014). Male Wistar rats (n=39 [ g]) were submitted to stereotaxic surgery for implantation of a cannula in the hilus of dentate gyrus of hippocampus. The animals received microinjections of saline (SAL, 1μL) or phlorizin (PZN, 50μg/μL) in the hippocampus followed 30 minutes later by pilocarpine (1.2mg/μL). Behavioral analysis of seizures was performed for 90 minutes during of status epilepticus (SE), according to Racine scale (1972). The animals were perfused after 24 hours or 15 days of SE and their brains were collected and processed. Neurodegeneration was evaluated by Fluoro-Jade (FJ) and FJ positive neurons (FJ+) were counted (ImageJ NIH). Results were expressed as mean±sem, compared by unpaired t test. SGLT1 inhibition increased the severity of seizures. After 24 hours of SE, PZN animals had a higher (p<0.05) number of FJ+ in the dentate gyrus (DG) (1205.0±116.9), hilus (277.3±23.2), CA3 (338.0±36.8) and CA1 (400.2±60.5) of hippocampus when compared to the same region of SAL group: DG (693.3±109.0), hilus (141.5±45.1), CA3 (170.2±61.1), CA1, (221.0±52.7). However, 15 days after SE, PZN animals had a decreased number (p<0.05) of FJ+ in CA1 (245.3±45.9) compared to the same region of SAL group (425.8±47.5). These data suggest that possibly the SGLT1 participates in the modulation of epileptogenic processes INTRACEREBROVENTRICULAR STREPTOZOTOCIN IMPAIRS SOCIAL BEHAVIOR IN WISTAR RATS: IMPLICATIONS FOR ALZHEIMER'S DISEASE ANNA CAROLINA PARRACHO MOTZKO SOARES; ROBSON CRISTIANO LILLO VIZIN; DANIEL MOREIRA- SILVA; MARIA CAMILA ALMEIDA; DANIEL CARNEIRO CARRETTIERO UNIVERSIDADE FEDERAL DO ABC, SAO BERNARDO DO CAMPO, SP, BRASIL. Streptozotocin (STZ), a chemical compound that promotes central insuline resistance, is used as an animal model for sporadic Alzheimer s disease (AD). In this study, we examine its influence in social behavior in Wistar rats (20 weeks old) treated with intracerebroventricular (icv) STZ (4 mg/kg) or its vehicle. Memory impairment (using Y-maze test) and social behavior were analyzed 30 days later. In Y-maze test, exploration time in known arms [unblocked (UA); start arm (SA)] and novel arm [blocked (BA)] was evaluated. In social behavior test, unknown rats were put in pairs and time spent in social interaction was evaluated for 5 min. Values are expressed as mean ± SEM (n = 4). Interaction time is expressed in percentage. Y-maze test demonstrate STZ-treated rats have no difference in time spent among arms (UA: ± 17.5 s; BA: 69.3 ± 10.7 min; SA: 80.3 ± 23.1 min), while control group (CG) spent less time in the UA (45.7 ± 4.5 s) as compared to BA (87.3 ± 5.2 s) and to SA (95.3 ± 7.1 s). Furthermore, STZ-treated animals spent more time within UA as compared to CG. In social behavior test, STZ treated animals (53.7 ± 2) interact less than CG (65.7 ± 0.7) - p < 0.05 for all tests. The relationship between STZ animal model and social behavior test was not well established in the literature. Our data show, for the first time, icv STZ impairs social behavior in Wistar rats. The result is a new piece in the complex puzzle of sporadic animal model for AD ESTRADIOL ATTENUATES HYPERPHAGIA CAUSED BY THE ACTIVATION OF 5-HT1A RECEPTORS IN THE LATERAL HYPOTHALAMIC AREA IN SATIATED RATS ANA PAULA DAMBROS TASCHETTO; LARISSA KOCHENBORGER; BRUNNO ROCHA LEVONE; RAFAEL APPEL FLORES; MOACIR SERRALVO FARIA; MARTA APARECIDA PASCHOALINI UFSC, FLORIANOPOLIS, SC, BRASIL. The aim of the study was evaluate the ingestive and not ingestive effects caused by injection into the lateral hypothalamic area (LHA) of the 8-OH-DPAT (receptor agonist 5-HT1A e 5-HT7, 6nmol), WAY (selective receptor antagonist 5-HT1A, 0,37 nmol) and MM77 (receptor antagonista 5-HT1A, 0,37 nmol) in ovariectomized rats (OVX) and ovariectomized treated with estradiol (OVX + E). Injections of 8-OH-DPAT in OVX rats significantly increase food intake duration and the frequency of this behaviour. When animals are treated with estradiol (E2), we did not observe a significant difference in these behaviours. The latency to food intake did not change with the different treatments in OVX or in OVX + E. When animals were pre-treated with the antagonists WAY10063 and MM 77, the hyperphagic effects evoked by 8-OH-DPAT were attenuate, as well the increase in the duration and frequency of this behaviour in untreated OVX rats. The another non ingestive behaviours (locomotion, immobility, non locomotor exploration, rearing and grooming) did not change in OVX or OVX + E. Water intake also remains unchanged in OVX or OVX + E, excluding a possible involvement of 5-HT1A receptors, present in the AHL, in the control of water intake. These results confirm the involvement of 5-HT1A receptors present in the AHL in the control of food intake suggesting that ovarian steroids interact with the serotoninergic system present in the AHL, attenuating the ingestive response after 5-HT1A receptors activation AN OVERVIEW OF THE WISTAR AUDIOGENIC RAT (WAR) STRAIN CONTRIBUTIONS TO PHYSIOLOGY AND NEUROSCIENCE: CHALLENGES AND OPPORTUNITIES NORBERTO GARCIA-CAIRASCO; EDUARDO UMEOKA FMRP-USP, RIBEIRAO PRETO, SP, BRASIL;. Epilepsy comorbidities, bring a new potential to the Wistar Audiogenic Rat (WAR) a geneticallyselected strain of rats suitable for epileptology, to be studied in neuropsychiatry, aging, endocrinology and cardio-respiratory regulation. At the Physiology Department of the FMRP-USP in 25 years we have produced over 53 generations of inbreeding: WAR animals being in the last five years: 689, 673 (Total 1.362). WARs are endogenously anxious with reduced exploration in the elevated plus maze/open field and are smaller than Wistars, with hyperplasic adrenal gland/higher levels of corticosterone, after exogenous ACTH injection. Naïve WARs display higher grooming behavior when compared to Wistars microinjected with oxytocin into the amygdala, so they have potential for studies of compulsive behavior. These alterations are additional to higher blood pressure and altered autonomic regulation of the cardiovascular system and highly compromised brainstem 5-HT nuclei associated to respiratory control. The Morris Water Maze revealed impaired reference memory in young WARs and the Novel Object Recognition test indicated deficit in short-term memory in middle age WARs. The additional detection of altered β- amyloid protein and phosphorylated-tau in the brain of WARs encourage the use of WARs as a model of Epilepsy-Alzheimer disease comorbidities. WARs are then a suitable model to study the complexity of normal brain function, as well as neuropsychiatric diseases, then we are donating WAR embryos to be available through the Rat Research ad Resource Center (RRRC) in Missouri, USA EFFECT OF FLUOXETINE ON INHIBITORY AVOIDANCE MEMORY IN ASTYANAX SP ANA PAULA FERREIRA; ANA AMÉLIA MAIA SILVA; LILIAM MIDORI IDE UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI, SAO JOAO DEL REI, MG, BRASIL. INTRODUCTION. The inhibitory avoidance involves the formation of a declarative memory in which the animal learns to inhibit an innate response to avoid an aversive stimulus. We investigated the effect of fluoxetine, a selective 5-HT reuptake inhibitor (SSRI), in learning and memory processes, in an inhibitory avoidance test in Astyanax sp. METHODS. The experimental apparatus consisted of a rectangular aquarium divided into two compartments (black and white), with a central door. After the fish crossed the line between compartments a 45 g weight was dropped in the black side. During training, this procedure was done seven times in a row. The animals were not treated or submitted to a single i.p. injection of saline (0.01 ml/g b.w.) or fluoxetine hydrochloride (10 or 100 ug/g b.w), 30 min before training. After that, the retention tests were performed 3 and 24 h after training. Latency to enter the black compartment was assessed and higher latencies were indicative of increased avoidance learning. RESULTS. The control animals showed an increase in the average of six training latencies, indicating learning (Friedman, followed by Dunn post-hoc comparison, P 0.001). The animals treated with 100 ug/g b.w. fluoxetine showed a significant increase in the latencies in the average latencies of 6 tests, 3 and 24 h after training, indicating retention (Kruskal-Wallis, followed by Dunn's Test; P=0.022; P=0.05; P=0.005). CONCLUSIONS. These results show that fluoxetine acts influencing the retention of inhibitory avoidance memory and cause an anxiolytic-like effect in Astyanax sp INHIBITORY AVOIDANCE LEARNING AND ARGYROPHILIC NUCLEOLAR ORGANIZER REGION OF DORSOMEDIAL TELENCEPHALIC NEURONS IN LEPORINUS MACROCEPHALUS ANA AMÉLIA MAIA SILVA; ANA PAULA FERREIRA; LILIAM MIDORI IDE UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI, SAO JOAO DEL REI, MG, BRASIL. INTRODUCTION. The cellular activity related to transcriptional activity may be evaluated by measuring the argyrophilic nucleolar organizer region (AgNOR). In this study we used the AgNOR neurohistochemical stain technique to assess morphological changes in dorsomedial telencephalic neurons in Leporinus macrocephalus submitted to inhibitory avoidance training. METHODS. The inhibitory avoidance apparatus consisted of a rectangular aquarium divided into two compartments (black and white), with a central door. After the fish crossed the line between compartments a 45 g weight was dropped in the black side. During training, this procedure was done seven times in a row. The behavioral retention tests were performed 3 and 15 days after training (n=10), and trained animals (n=8) or submitted to a similar non-contingent behavioral procedure (control group, n=8) were morphologically evaluated. The brain sections were observed under an optical microscope at 1000x magnification and the images captured and analyzed using ImageJ software. RESULTS. The animals showed an increase in the average of six training latencies, indicating learning (Friedman, followed by Dunn post-hoc comparison, P 0.001). The number and the relative area of AgNOR in the dorsomedial telencephalic neurons analyzed in the five trained animals and three in the control group were statistically similar. CONCLUSIONS. These partial results allow to conclude that L. macrocephalus tested do not show specific neuronal changes after undergoing inhibitory avoidance task SEROTONERGIC ACTIVATION IN THE NTS INHIBITS WATER INTAKE INDUCED BY CENTRAL ANGIOTENSIN II RODRIGO ANDERSON TOMEO; JOSÉ VANDERLEI MENANI; PATRICIA MARIA DE PAULA UNIVERSIDADE ESTADUAL PAULISTA, ARARAQUARA, SP, BRASIL. The nucleus of the solitary tract (NTS), the primary site of peripheral osmoreceptor and cardiovascular afferences, is suggested to receive important inhibitory signals involved in the control of water and sodium intake, however, the neurotransmitters involved in this mechanism are not known. Central serotonergic mechanisms are involved in the control of sodium and water intake and immunohistochemical studies provided evidence for serotonergic receptors in the NTS. Therefore, in the present study, we investigated the effects of DOI (a serotonergic 5-HT2A/2C receptor agonist) injected into the NTS on water intake induced by intracerebroventricular (icv) angiotensin II (ANG II). Male Holtzman rats ( g, n=11) with bilateral stainless steel cannulas implanted into the NTS and into the lateral ventricle (LV) were used. Thirsty was induced by icv ANG II (50 ng/1 µl) administered 45 minutes before bilateral injections of DOI (2.5 µg/100 nl) or vehicle into the NTS (CEUA FOAR 14/2013). Bilateral injections of the serotonergic 5-HT2A/2C 20

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